Objective:To establish a method to detect food allergen based on Latex-enhanced immune turbidimetry ( PETIA) and apply to clinic.Methods: The PETIA method was used for evaluating the detection system including standard curve , detection-limit,stability, intra-assay and inter-assay precision and cross-reactivity,clinical normal serum specimens and clinical allergic diseases serum specimens were measured to evaluated the perspective of the clinical application .Results: The standard curve :Y=837.1 x2-125.2x+10.036, linear range: 0.0-400 U/ml, the correlation coefficient of the standard curve was 0.996, the intrabatch and interbatch precision was<10%.The normal reference range was≤33.5 U/ml,the AUC of ROC curve was 0.957,the sensitivity was 89.61%,The specificity was 65.22%, the accuracy was 84.00%, and the positive predicted value was 89.61%, the negative predicted value was 65.22%.the test results have strong correlation with ELISA ( r=0.890 2 ) .Conclusion: The PETIA method for detecting food allergen achieved corresponding clinical application standards and may be used for the diagnosis of food allergies .

Objective:to investigate clinical significance of serum and urine NGAL (Neutrophil gelatinase-associated lipocalin) in the early kidney damage with primary hypertension.Methods:According to UAER ( urinary microalbumin excreting rate ) ,we divided 90 patients with primary hypertension into three groups (<30 mg/24 h,30-300 mg/24 h and >300 mg/24h),and selected 30 healthy people as the control group.Serum and urine NGAL and cystatin C ,serum creatinine,urea nitrogen,high sensitive C reactive protein , transferrin,and basis of blood pressure were detected and followed up one year.Results:Compared with healthy group ,GFR( glomerular filtration rate ) and serum NGAL were decreased significantly in medium and severe proteinuria groups , while urine NGAL was increased.Conclusion:Serum and urine NGAL have been a clear trend changes in kidney damage ,which could be used as a reliable indicator in monitoring renal function of patients with primary hypertension.

Objective Using hybridoma technique and screened hybridoma cell strains stably, efficiently secreted anti glycosylated hemoglobin monoclonal antibody to provide specific material for the development of glycosylated hemoglobin ELISA kit. Methods The immune antigen was prepared by maleimide method, multi-level immune mice by BALB/c,through cell culture fusion, screening of hybridoma cell culture medium HAT, ammonium sulfate salting out method and G protein chromatography. Monoclonal antibody subclasses were identified by monoclonal antibody subtype identi-fication Kit operation. Results Through cell fusion, screening and cloning culture, etc., the final selection screened 1 strain stably secreting specific antibody hybridoma cell line, named N5B4; cell culture supernatant liquid was 1:5000, ascites titer was 1:100 million; a standard curve to calculate the concentration in the sample human glycat ed hemoglobin was 99.2%. Conclusion After KET monoclonal antibody cell line to obtain a high specificity and high sen-sitivity of screening.