BACKGROUND:Induced pluripotent stem cells research has become a hot topic nowadays, because it can avoid the immune rejection and ethical issues in stem cellresearch and have characteristics is similar to the embryonic stem cells.
OBJECTIVE:To review prospect and progress of induced pluripotent stem cells, and to find out some problems about the induced pluripotent stem cells in inducing method, efficiency, safety and application which need to be solved.
METHODS:Kjmed database was searched by the first author using key words of“induced pluripotent stem cells, efficiency, disease model, clinical application”in English to retrieve relevant articles published from January 2003 to June 2013. Literatures addressing induced pluripotent stem cells were included, and the repetitive researches were excluded.
RESULTS AND CONCLUSION:A total of 112 documents were retieved, and final y 32 articles were retained after deleting unrelated and repetitive ones. Induced pluripotent stem cells have very high application value and prospects in the somatic celltransplantation, tissue engineering, drug screening and establishment in vitro of disease models. But this technology is stil in the stage of research and exploration, and there are many technical problems that need to be solved.

Unique totipotency of embryonic stem cell make it great value.Embryonic stem cells have received extensive attention from the discovery,research to application.This paper outlined the discovery of embryonic stem cell and the challenges faced in the progress of the study and research process,focusing on the ethical issues.Meanwhile,this paper analyzed the attitude of various governments,the status of the relevant laws and regulations in order to discuss the prospects of stem cell re

Objective To observe the clinical efficacy of Tiao Shen Kai Yu (regulating mind and anti-depression) needling plus mental intervention in treating mild post-stroke depression. Method A total of 100 eligible subjects with mild post-stroke depression were randomized into a control group and an observation group, 50 cases in each group. The two groups were both intervened by basic treatment, while the control group was additionally given mental intervention and the observation group was given Tiao Shen Kai Yu needling plus mental intervention. The two groups were treated once a day, 5 times a week, for 30 d in total. The Self-rating Depression Scale (SDS) and Hamilton Depression Scale (HAMD) were observed before and after the treatment. Result The HAMD scores were both significantly improved in the two groups after the treatment (P<0.01), and the between-group difference was statistically significant (P<0.01). The SDS scores were significantly improved after the treatment in both groups (P<0.01), and the between-group difference was statistically significant (P<0.01). Conclusion Tiao Shen Kai Yu needling plus mental intervention can more effectively improve mild post-stroke depression than single mental intervention.

Objective To study the clinical effect of minocycline hydrochlorideointment on the treatment of patients with retrograde pulpitis.Methods From January 2015 to December 2016 in Huzhou central hospital, 90 cases with retrograde pulpitis as the research object were divided into two groups, the control group and the observation group, 45 cases in each group.The control group were received conventional treatment combined with iodine glycerin, and the observation group were given conventional treatment combined with minocycline hydrochloride ointment.The clinical data were compared in the two groups.Results After treatment, plaque index(0.91±0.30), gingival bleeding index(2.11±1.32), explore clinical depth(4.58±1.19)and attachment loss(3.06±1.85)in the observation group were better than the plaque index(1.38±0.49), gingival bleeding index(3.07±1.83),explore clinical depth(4.87±1.49)and attachment loss(5.18±1.39)in the control group, the difference between the two groups was statistically significant(P< 0.05);the clinical effect 95.6% in the observation group was better than 80.0% in the control group(P< 0.05).The adverse reaction rate was 6.7% in the observation group, which was lower than 17.8% in the control group(P< 0.05).Conclusion It that the conventional method combined with minocycline hydrochloride ointment on the treatment of retrograde pulpitis has significant clinical effect, can effectively improve the clinical symptoms of patients, is worthy of clinical application.

Objective To investigate the effect of vitamin D combined with iron succinate on children with premature anemia .Methods 66 patients with premature anemia from November 2013 to June 2015 in our hospital were selected and randomly divided into the control group and the experiment group, with 33 cases in each group.The patients in the both groups were give regular treatment, the experimental group was given the protein succinate oral solution (phenipril) 1.5 mL/kg on the basis of conventional treatment, Vitamin D drops ,one times a day.Two groups were treated continuously for 60 days.The hemoglobin, serum iron, ferritin, liver and kidney function and adverse reactions were compared before and after treatment.Results The hemoglobin, serum iron and ferritin levels in two groups were higher after treatment(P<0.05),compared with the control group,the hemoglobin, serum iron and ferritin levels in the experimental group were higher(P<0.05).Compared with before treatment ,the levels of serum ALT, AST, Cr and BUN in the two groups were not statistically significant.There was no significant difference in adverse reactions between two groups.Conclusion Vitamin D and iron proteinsuccinylate combined treatment of anemia in premature infants clinical curative effect, improve the body hemoglobin, serum iron and ferritin levels, without causing damage to liver and kidney function, drug safety.

Objective To establish a basic information database of hospital medical records management and improve the management process.Methods The system was developed with C# programming language,basic information from Oracle 10g databases of HIS and electronic medical record system (EMRS),which stored corresponding information during the process into SQL 2005 database and generated early warning information based on statistical analysis on medical records.Results The system realized standardized filing of medical records,synchronized filing of electronic medical record and the first page of medical record,normalized lending of medical record,auto generation of statistical reports,auto alarming in case of standard-exceeded information and etc,which could be used as a platform for medical record management.Conclusion The system enhances the efficiency and management of medical record section,and thus is worthy promoting practically.

Objective To investigate the current working status and explore the relationship among job stress, job burnout and organizational commitment in ICU nurses. Methods By using cluster random sampling method, 233 nurses from 5 general hospitals were selected to accept investigation by Commitment Questionnaire and Nurses' general situation questionnaire. Results The job stress and job burnout were both significant negatively correlated with organizational commitment (r=-0.33, P<0.01;r=-0.22, P < 0.01).The management issues and interpersonal relationships and decreased personal accomplishment both had significant impact on affective commitment and continuance commitment of ICU nurses (r=0.539, P < 0.01; r=0.387, P < 0.01). The management issues and interpersonal relationships, professional and career issues and emotional exhaustion had significant predictive power on normative commitment(r=0.458,P < 0.01). Conclusion It generally was in a higher level of job stress in ICU nurses, which indicates that hospital mangers should try to eliminate the causes ,assist them to reduce job stress and relieve the job burnout.

Objective To evaluate the correlation between Narcotrend index (NI) and the depth of anesthesia during inhalation anesthesia with sevoflurane and compare NI with BIS values in patients.Methods Sixtytwo ASA physical status Ⅰ or Ⅱ patients,aged 25-60 yr,weighing 44-100kg,scheduled for elective surgeries under general anesthesia,were enrolled in the study.Sevoflurane inhalation was started at 5 min after endotracheal intubation.When the end-tidal concentration of sevoflurane (ETsevo) reached 0.6％,0.9％,1.3％,1.6％,2.1％,2.4％,2.9％,3.2％,3.8％ and 4.2％,NI and BIS values were recorded simultaneously.Results The correlation coefficients between ETsevo and NI and BIS values were-0.627 (r1) and-0.669 (r2) respectively when ETsevo ＜ 2.9％,and-0.608 (r3) and-0.230 (r4) respectively when 2.9％ ≤ ETsevo ≤4.2％ (P ＜ 0.01).Comparison of the absolute value of correlation coefficients:no significant difference was found between r1,r2 and r3 (P ＞ 0.05) ; r1,r2 and r3 were significantly greater than r4 (P ＜ 0.01).Conclusion When ETsevo 4.2％,NI values can accurately reflect the depth of anesthesia; when 2.9％ ≤ ETsevo ≤4.2％,the accuracy of NI values in monitoring the depth of anesthesia is superior to that of BIS values.

Objective To investigate the effect of lliac fascia compartment block on early analgesia of patients during performed the whole knee replacement.Methods Forty patients undergoing single whole knee replacement were randomly divided into group A and B,20 cases in each group.Before anesthesia,patients in group A underwentthree in one femoral nerve block guided by nerve stimulator with 20 ml of 0.5％ ropivacaine.Patients in group B underwent lliac fascia compartment block with 25 ml of 0.5％ ropivacaine.Visual analogue score (VAS) during rest and movement were recorded at 0 (T0),0.5 (T1),1 (T2),2 (T3),4 (T4),6 (T5),and 8 h (T6) after a patient fully awoke from surgery.Results In group B,Resting VAS scoresofpatientswere(4.5±0.7),(4.5 ±0.6),(4.2 ±0.8),(4.4 ±0.5),(4.3 ±0.7),(4.4 ±0.6),(5.2 ±0.6) at T0-T6 time point,and moving VAS were(4.7 ±0.6),(4.4 ±0.7),(4.4 ±0.5),(4.3 ±0.6),(4.4±0.6),(5.0 ±0.7),(5.1 ±0.6),all higher than that of group A((1.5 ±0.6),(1.7 ±0.6),(1.7 ± 0.6),(1.7 ±0.6),(1.9 ±0.6),(2.0 ±0.6),(2.2 ± 0.5) and(1.6 ±0.6),(2.0 ±0.6),(1.8 ±0.6),(1.7 ± 0.6),(2.0 ± 0.6),(2.3 ± 0.6),(2.5 ± 0.7),and there was significant difference (resting VAS∶ F (between group) =17.672,P ＜ 0.05,F(within group) =5.062,P ＜ 0.001,F(interaction) =1.443,P =0.143) ; Moving VAS:F (between group) =8.183,P ＜ 0.001,F (within group) =7.742,P ＜ 0.001,F (interaction) =0.85,P =0.424).Conclusion Iliac fascia compartment block had proved with early analgesic effect in patients with total knee replacement.Iliac fascia block is simple,safe,and can be performed without nerve stimulator.Therefore iliac fascia block has a certain clinical application.

To explore the significance of the application of standardized special staining technique in pathologic diagnosis of pulmonary fungal infectious diseases.Methods Final pathologic diagnosis of 104 cases pulmonary fungus infection disease in Beijing Chaoyang hospital from September 2011 to March 2016 were selected;HE staining only,HE staining combined with the traditional manual special staining method PAS and hexamine silver,and HE staining combined with automatic special staining PAS and hexamine-silver were used and compared.The two kinds of special staining technology were compared;the microscopic observation,analysis results (all the first staining results,not including the results of complex staining),the results on the basis of final pathologic diagnosis were also compared with the clinical preliminary diagnosis.Results Lung fungal infectious disease diagnosis rate and fungal classification rate,from low to high order consistence,showed that for the primary clinical diagnosis (29.8％ and 19.2％),HE staining (32.7％ and 32.7％),HE staining combined with traditional manual special staining method PAS and hexamine silver (90.4％ and 87.5％),and HE staining combined with automatic special staining PAS and hexamine-silver (98.1％ and 94.2％).The four methods were statistically significant on two aspects (P ＜ 0.01,P ＜ 0.01);the fourth method was significantly different from the first two (P ＜ 0.01,P ＜ 0.01).The fourth method was significantly different from the third kind of diagnosis rate (P ＜ 0.05),typing rate was no significant difference (P ＞ 0.05).But automatic special dyeing method of PAS and hexamine silver steps were more simple,with standardized chemical reagents,no artificial and environmental factors,short time-consuming,and less number of dropping-off and restaining of the section.Conclusion HE staining and its combination with automatic special staining of PAS and hexamine silver are much more standardized,and help to improve the diagnosis of pulmonary fungal infectious diseases and fungal classification rate.