BACKGROUND:Induced pluripotent stem cells research has become a hot topic nowadays, because it can avoid the immune rejection and ethical issues in stem cellresearch and have characteristics is similar to the embryonic stem cells.
OBJECTIVE:To review prospect and progress of induced pluripotent stem cells, and to find out some problems about the induced pluripotent stem cells in inducing method, efficiency, safety and application which need to be solved.
METHODS:Kjmed database was searched by the first author using key words of“induced pluripotent stem cells, efficiency, disease model, clinical application”in English to retrieve relevant articles published from January 2003 to June 2013. Literatures addressing induced pluripotent stem cells were included, and the repetitive researches were excluded.
RESULTS AND CONCLUSION:A total of 112 documents were retieved, and final y 32 articles were retained after deleting unrelated and repetitive ones. Induced pluripotent stem cells have very high application value and prospects in the somatic celltransplantation, tissue engineering, drug screening and establishment in vitro of disease models. But this technology is stil in the stage of research and exploration, and there are many technical problems that need to be solved.

Objective To observe the clinical efficacy of Tiao Shen Kai Yu (regulating mind and anti-depression) needling plus mental intervention in treating mild post-stroke depression. Method A total of 100 eligible subjects with mild post-stroke depression were randomized into a control group and an observation group, 50 cases in each group. The two groups were both intervened by basic treatment, while the control group was additionally given mental intervention and the observation group was given Tiao Shen Kai Yu needling plus mental intervention. The two groups were treated once a day, 5 times a week, for 30 d in total. The Self-rating Depression Scale (SDS) and Hamilton Depression Scale (HAMD) were observed before and after the treatment. Result The HAMD scores were both significantly improved in the two groups after the treatment (P<0.01), and the between-group difference was statistically significant (P<0.01). The SDS scores were significantly improved after the treatment in both groups (P<0.01), and the between-group difference was statistically significant (P<0.01). Conclusion Tiao Shen Kai Yu needling plus mental intervention can more effectively improve mild post-stroke depression than single mental intervention.

Objective To study the clinical effect of minocycline hydrochlorideointment on the treatment of patients with retrograde pulpitis.Methods From January 2015 to December 2016 in Huzhou central hospital, 90 cases with retrograde pulpitis as the research object were divided into two groups, the control group and the observation group, 45 cases in each group.The control group were received conventional treatment combined with iodine glycerin, and the observation group were given conventional treatment combined with minocycline hydrochloride ointment.The clinical data were compared in the two groups.Results After treatment, plaque index(0.91±0.30), gingival bleeding index(2.11±1.32), explore clinical depth(4.58±1.19)and attachment loss(3.06±1.85)in the observation group were better than the plaque index(1.38±0.49), gingival bleeding index(3.07±1.83),explore clinical depth(4.87±1.49)and attachment loss(5.18±1.39)in the control group, the difference between the two groups was statistically significant(P< 0.05);the clinical effect 95.6% in the observation group was better than 80.0% in the control group(P< 0.05).The adverse reaction rate was 6.7% in the observation group, which was lower than 17.8% in the control group(P< 0.05).Conclusion It that the conventional method combined with minocycline hydrochloride ointment on the treatment of retrograde pulpitis has significant clinical effect, can effectively improve the clinical symptoms of patients, is worthy of clinical application.

Unique totipotency of embryonic stem cell make it great value.Embryonic stem cells have received extensive attention from the discovery,research to application.This paper outlined the discovery of embryonic stem cell and the challenges faced in the progress of the study and research process,focusing on the ethical issues.Meanwhile,this paper analyzed the attitude of various governments,the status of the relevant laws and regulations in order to discuss the prospects of stem cell re

Objective To evaluate the correlation between Narcotrend index (NI) and the depth of anesthesia during inhalation anesthesia with sevoflurane and compare NI with BIS values in patients.Methods Sixtytwo ASA physical status Ⅰ or Ⅱ patients,aged 25-60 yr,weighing 44-100kg,scheduled for elective surgeries under general anesthesia,were enrolled in the study.Sevoflurane inhalation was started at 5 min after endotracheal intubation.When the end-tidal concentration of sevoflurane (ETsevo) reached 0.6％,0.9％,1.3％,1.6％,2.1％,2.4％,2.9％,3.2％,3.8％ and 4.2％,NI and BIS values were recorded simultaneously.Results The correlation coefficients between ETsevo and NI and BIS values were-0.627 (r1) and-0.669 (r2) respectively when ETsevo ＜ 2.9％,and-0.608 (r3) and-0.230 (r4) respectively when 2.9％ ≤ ETsevo ≤4.2％ (P ＜ 0.01).Comparison of the absolute value of correlation coefficients:no significant difference was found between r1,r2 and r3 (P ＞ 0.05) ; r1,r2 and r3 were significantly greater than r4 (P ＜ 0.01).Conclusion When ETsevo 4.2％,NI values can accurately reflect the depth of anesthesia; when 2.9％ ≤ ETsevo ≤4.2％,the accuracy of NI values in monitoring the depth of anesthesia is superior to that of BIS values.

Objective To investigate the effect of life style combined with dietary intervention on non-alcoholic fatty liver disease(NAFLD).Method One hundred and twenty five subjects of NAFLD with overweight and obesity,aged 30-60 years,were enrolled in the study from the group for physical examination in Health Administration Center of our hospital during 2006-2007.They were diagnosed to be 48 mild,70 moderate and 7 severe cases by ultrasonic tomography.A regime of dietary intervention combined with exercise was planned according to 3 d dietary survey before intervention.They were asked to follow the therapeutic menus and exercise instruction during 12 mon of the study.We followed up the subjects and gave them instructions regularly in the whole period of study.Results After 12 mon interven-tion,the body weight(BW),body mass index(BMI),waist-hip ratio(WHR),serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C) and alanine glutamate aminotransferase(ALT) of the subjects were decreased significantly.High density lipoprotein cholesterol(HDL-C) was not changed.By ultrasonic tomography,16% of the subjects recovered to normal,and 77 moderate to severe cases deceased to 31,with reduction rate 59.7%.Conclusion Good therapeutic effects were observed after 12 mon of dietary intervention combined with rational exercise.

Objective To investigate the effect of lliac fascia compartment block on early analgesia of patients during performed the whole knee replacement.Methods Forty patients undergoing single whole knee replacement were randomly divided into group A and B,20 cases in each group.Before anesthesia,patients in group A underwentthree in one femoral nerve block guided by nerve stimulator with 20 ml of 0.5％ ropivacaine.Patients in group B underwent lliac fascia compartment block with 25 ml of 0.5％ ropivacaine.Visual analogue score (VAS) during rest and movement were recorded at 0 (T0),0.5 (T1),1 (T2),2 (T3),4 (T4),6 (T5),and 8 h (T6) after a patient fully awoke from surgery.Results In group B,Resting VAS scoresofpatientswere(4.5±0.7),(4.5 ±0.6),(4.2 ±0.8),(4.4 ±0.5),(4.3 ±0.7),(4.4 ±0.6),(5.2 ±0.6) at T0-T6 time point,and moving VAS were(4.7 ±0.6),(4.4 ±0.7),(4.4 ±0.5),(4.3 ±0.6),(4.4±0.6),(5.0 ±0.7),(5.1 ±0.6),all higher than that of group A((1.5 ±0.6),(1.7 ±0.6),(1.7 ± 0.6),(1.7 ±0.6),(1.9 ±0.6),(2.0 ±0.6),(2.2 ± 0.5) and(1.6 ±0.6),(2.0 ±0.6),(1.8 ±0.6),(1.7 ± 0.6),(2.0 ± 0.6),(2.3 ± 0.6),(2.5 ± 0.7),and there was significant difference (resting VAS∶ F (between group) =17.672,P ＜ 0.05,F(within group) =5.062,P ＜ 0.001,F(interaction) =1.443,P =0.143) ; Moving VAS:F (between group) =8.183,P ＜ 0.001,F (within group) =7.742,P ＜ 0.001,F (interaction) =0.85,P =0.424).Conclusion Iliac fascia compartment block had proved with early analgesic effect in patients with total knee replacement.Iliac fascia block is simple,safe,and can be performed without nerve stimulator.Therefore iliac fascia block has a certain clinical application.

Objective To study the status of knowledge-attitude-practice (KAP) of environmental health among urban and rural residents and the influencing factors. Methods Totally,4 607 urban and rural residents from Lanzhou,Taiyuan,Shanghai and Qingdao were recruited and investigated by the self-designed questionnaire from July 3rd to August 10th 2007. In the questionnaire,a certain number of related questions were designed mainly on environmental health knowledge,attitude and practice. The datas were analyzed with SPSS13.0 software. Results The average scores of environmental health knowledge,attitude and practice of urban and rural residents were 4.14?1.30,4.88?0.85 and 4.05?1.11 respectively. The main influencing factors of environmental health knowledge were education background (?=0.220,P

Objective To observe the effect of diet control combined with exercise on hyperlipi-demia,hyperglycemia and high blood pressure in overweight subjects. Method One hundred and four subjects (75 men and 29 women )were chosen from 6789 persons for medical examinations at health administration centre of our hospital from August to December in 2005. All of them met the following requirements: age 35-60,BMI value 24—28,high blood lipids ,high blood glucose or high blood pressure ,never using any drugs and excluding diseases with defects of heart ,liver or renal function and osteoarthritis diseases affecting physical activities. They were required to follow the plan of diet control and exercise for 24 w. The changes of their body weights (BW) ,body mass index (BMI),waist-hip ratio (WHR),total cholesterol (TC),total triglyceride (TG),high-density lipoprotein (HDL-C),low-density lipoprotein (LDL-C),fasting plasma glucose(FPG),2 hours plasma glucose (2 hPG),glycosylated haemoglobin (HbA1c),systolic blood pressure (SBP) and diastolic blood pressure (DBP) were observed . Results After 24 w intervention by diet control and exercise ,the BW ,BMI,FPG,2hPG ,HbAlc ,TC ,TG,LDL-C and SBP of the subjects studied were decreased drastically. HDL-C and DBP were not changed. Conclusion Scientific and reasonable diet pattern and rational exercise can lower the hyperlipidemia ,hyperglycemia and hypertension in overweight subjects.

To explore the significance of the application of standardized special staining technique in pathologic diagnosis of pulmonary fungal infectious diseases.Methods Final pathologic diagnosis of 104 cases pulmonary fungus infection disease in Beijing Chaoyang hospital from September 2011 to March 2016 were selected;HE staining only,HE staining combined with the traditional manual special staining method PAS and hexamine silver,and HE staining combined with automatic special staining PAS and hexamine-silver were used and compared.The two kinds of special staining technology were compared;the microscopic observation,analysis results (all the first staining results,not including the results of complex staining),the results on the basis of final pathologic diagnosis were also compared with the clinical preliminary diagnosis.Results Lung fungal infectious disease diagnosis rate and fungal classification rate,from low to high order consistence,showed that for the primary clinical diagnosis (29.8％ and 19.2％),HE staining (32.7％ and 32.7％),HE staining combined with traditional manual special staining method PAS and hexamine silver (90.4％ and 87.5％),and HE staining combined with automatic special staining PAS and hexamine-silver (98.1％ and 94.2％).The four methods were statistically significant on two aspects (P ＜ 0.01,P ＜ 0.01);the fourth method was significantly different from the first two (P ＜ 0.01,P ＜ 0.01).The fourth method was significantly different from the third kind of diagnosis rate (P ＜ 0.05),typing rate was no significant difference (P ＞ 0.05).But automatic special dyeing method of PAS and hexamine silver steps were more simple,with standardized chemical reagents,no artificial and environmental factors,short time-consuming,and less number of dropping-off and restaining of the section.Conclusion HE staining and its combination with automatic special staining of PAS and hexamine silver are much more standardized,and help to improve the diagnosis of pulmonary fungal infectious diseases and fungal classification rate.