1.An exploration on new management mode for biomedicine key laboratory: to realize the sharing of space and equipment under network management mode with the simplified democratic centralism
Chinese Journal of Medical Science Research Management 2016;29(1):28-31
The current situation in basic biomedicine research laboratory is limited space,crowded instruments and equipment,and with heavy duty on lab management.Over the past decade we have applied the effective implementation of the laboratory equipment and space sharing,with a democratic centralism simplify lab management.The pattern of professors under the leadership of director (or principal investigator) collective leadership,and combined centralized management mode with the laboratory equipment and space sharing,and simplified mode that the professors or principal investigators are responsible,to make the use of lab space and equipment more rational,which also improve the effectiveness of lab management,more cohesive of lab staff,lab work running stable and smooth.
2.Pharmacodynamics of anti-diarrhea medicine of the compound catechu antidiarrheal ointment
Ting GONG ; Feifei DU ; Suxi LIAO ; Ming GAO
Chongqing Medicine 2013;(30):3615-3616
Objective To observe the gastrointestinal function influence in the compound catechu antidiarrheal ointment when acted on normal and diarrheic mice .Methods The antidiarrheal function of the compound catechu antidiarrheal ointment was ob-served through the mice small intestinal propulsion experiment ,gastric emptying experiment and mice diarrhea situation influence test of senna .Results This ointment could reduce the mice small intestine propulsion rate which significantly restrained the mice small intestine propulsion ;Also it could improve the residual rate of mice stomach contents ,significantly restrained their gastric emptying velocity .Conclusion The compound catechu antidiarrheal ointment has strong and lasting effect on stopping diarrhea .
3.Effect of Vagus Nerve Stimulation on Wake-promoting and Expression of γ-aminobutyric Acid b1 Receptor in Prefrontal Cortex of Coma Rats post Traumatic Brain Injury
Chengcheng LIAO ; Zhen FENG ; Feifei HUANG ; Qin CHEN
Chinese Journal of Rehabilitation Theory and Practice 2017;23(9):1037-1042
Objective To investigate the wake-promoting effect of vagus nerve stimulation (VNS) on coma rats after traumatic brain in-jury (TBI), and the related mechanism. Methods A total of 168 healthy Sprague-Dawley rats were randomly divided into blank group, TBI group, antagonist group and VNS group, 42 rats in each group. The latter three groups were established TBI model with impact, and the rats in coma at least 30 minutes were included. VNS group accepted VNS, the antagonist group were injected intralateroventricularly Orexin A receptor 1 (OXR1) antagonist SB334867, and TBI group accepted sham VNS. Their behaviors were observed to determine the level of con-sciousness six, twelve and 24 hours after intervention, while the expression ofγ-aminobutyric acid b1 receptor (GABAb1R) in prefrontal cortex was detected with immunohistochemistry and Western blotting. Results There were 42 rats in the blank group, 11 rats in TBI group, 13 rats in the antagonist group, and 28 rats in VNS group awakened finally. The expression of GABAb1R in prefrontal cortex ranged as TBI group, antagonist group, blank group and VNS group from more to less twelve and 24 hours after intervention under Western blotting (F>60.412, P<0.001), and it ranged as TBI group, antagonist group, VNS group and blank group under immunohistochemistry (H=15.121, P=0.002), with no significant difference among time points (H=3.028, P=0.220). Conclusion VNS can promote waking from coma in rats after TBI, which may relate with the decrease of GABAb1R in prefrontal cortex that induced by Orexin A.
4.Gene diagnosis of X-linked spondyloepiphyseal dysplasia tarda
Li WANG ; Feng YAO ; Shixiu LIAO ; Yingtai WANG ; Yanli YANG ; Feifei HUANG
Chinese Journal of Laboratory Medicine 2010;33(6):527-530
ObjectiveTo investigate the molecular pathogenesis of a pedigree of X-linked spondyloepiphyseal dysplasia atarda (SEDL) and to establish methods of gene diagnosis. Methods Clinical diagnosis was made based on height measurement, radiological examination and pedigree analysis. Peripheral blood samples of relevant family members were collected. After genomic DNA extraction, single strand conformation polymorphism (SSCP) followed with DNA sequencing was used to detect SEDL gene exons 36. Microsatellite marker DXS16 was selected for linkage analysis. Results The abnormal electrophoretic bands were detected in exon 4 of probands by PCR-SSCP. A c. 218C > T mutation in exon 4 of SEDL gene was found in three probands, which resulted in a change in amino acid sequence S37L. The heterozygous exon 4 mutation was identified in three carriers, but not in healthy individuals, and no mutations were detect in exon 3, 5 and 6 of probands. Three unmarried young females (Ⅲ10, Ⅳ6 and Ⅳ7) were found to harbor the mutation by DNA sequencing analysis. ConclusionsA c. 218C > T missense mutation in exon 4 of SEDL gene is the cause of molecular pathogenesis of the pedigree. SSCP and DNA sequencing can be used for prenatal gene diagnosis.
5.Hemostatic effect and mechanism of a non-polysaccharide fraction of Bletilla striata
Feifei ZHAO ; Xin YANG ; Dan XU ; Li DONG ; Jing LI ; Yonglin WANG ; Shanggao LIAO
Chinese Pharmacological Bulletin 2016;32(8):1121-1126
Aim To understand the hemostatic effect of a non-polysaccharide fraction of Bletilla striata ( BS-80EE) and to clarify its mechanism of action .Methods The non-polysaccharide fraction ( BS-80 EE ) was prepared by passing the 95%ethanol extract of Bletilla striata through a D101 macroporous resin column elu-ted first with water and then with 80%ethanol.Bleed-ing time ( BT ) and clotting time ( CT ) of heparinized mice were employed as indicators for evaluating the he-mostatic effect of BS-80EE.The mechanism of action was investigated through observing the effect of BS-80 EE on platelet aggregation induced by adenosine diphosphate ( ADP) in rats with nephelometry and tes-ting the effect of BS-80EE on the thrombin time(TT), prothrombin time(PT), activated partial thromboplas-tin time(APTT), fibrinogen(FIB), P-selectin(P-S), thrombin-antithrombin complex ( TAT ) , D-dimer ( D-D) and plasminogen activator inhibitor-1 ( PAI-1 ) . Results BS-80 EE significantly shortened the CT and BT( P<0.01 or 0.05 ) of heparin mice in a dose-de-pendent manner; groups of all doses significantly re-duced the rat TT ( P <0.01 or 0.05 ) , and the high-dose group significantly increased the FIB content ( P<0.05); the mid-dose group and high-dose groups of BS-80EE significantly increased the contents of P-S, TAT and PAI-1 , while reduced the D-D production in rats ( P <0.01 ); although dose-dependent reductions of APTT and PT were observed for each treatment-group, no significance was observed .Conclusion BS-80EE possess pronounced hemostatic effect by promo-ting platelet aggregation and coagulation .
6.Effect of cardiopulmonary bypass for 30 min on structure and mechanical properties of erythrocyte membrane surface
Cai NIE ; Gaoming SHE ; Yalan LI ; Donghua HU ; Jieke TANG ; Jing LIAO ; Ting ZHOU ; He TIAN ; Feifei WANG
Chinese Journal of Pathophysiology 2015;(9):1611-1616
AIM:To observe and analyze the effect of cardiopulmonary bypass ( CPB) for 30 min on surface ultra-structure and mechanical properties of the erythrocyte membrane by atomic force microscopy (AFM).METHODS:Ten cases of elective patients in cardiac surgery were selected in the study and divided into control ( CON) group and CPB group.The central venous blood (2 mL) before surgery and 30 min after CPB was collected with heparin anticoagulation . The non-circular red blood cells were counted under a stand fluorescence microscope .AFM was used to examine the ultra-structure of the membrane surface and measure the force curve of the erythrocytes .RESULTS:The percentage of non-cir-cular red blood cells in CPB group showed no statistically significant differences as compared with CON group .AFM images showed that the significant differences of membrane surface concave and convex , evenness , particle distribution , the sur-face average roughness (Ra), the surface root mean square roughness (Rq) and cell membrane adhesion between CPB group and CON group were observed .However, the membrane deformation resilience and curve slope had no significant difference between the 2 groups.CONCLUSION:Cardiopulmonary bypass for 30 min changes the morphology and ultra-structure of the erythrocyte membrane surface , and increases the adhesion between cells .
7.Application of chromosome microarray analysis for fetuses with multicystic dysplastic kidney.
Feifei CHEN ; Tingying LEI ; Fang FU ; Ru LI ; Yongling ZHANG ; Xiangyi JING ; Xin YANG ; Jin HAN ; Li ZHEN ; Min PAN ; Can LIAO
Chinese Journal of Medical Genetics 2016;33(6):752-757
OBJECTIVETo explore the genetic etiology of fetuses with multicystic dysplastic kidney (MCDK) by chromosome microarray analysis (CMA).
METHODSSeventy-two fetuses with MCDK were analyzed with conventional cytogenetic technique, among which 30 fetuses with a normal karyotype were subjected to CMA analysis with Affymetrix CytoScan HD arrays by following the manufacturer's protocol. The data was analyzed with ChAS software.
RESULTSConventional cytogenetic technique has revealed three fetuses (4.2%) with identifiable chromosomal aberrations. CMA analysis has detected pathogenic CNVs in 5 fetuses (16.7%), which included two well-known microdeletion or microduplication syndromes, i.e., 17q12 microdeletion syndrome and Williams-Beuren syndrome (WBS) and three submicroscopic imbalances at 4q35.2, 22q13.33, and 1p33. PEX26, FKBP6, TUBGCP6, ALG12, and CYP4A11 are likely the causative genes.
CONCLUSIONCMA can identify the submicroscopic imbalances unidentifiable by conventional cytogenetic technique, and therefore has a significant role in prenatal diagnosis and genetic counseling. The detection rate of pathogenic CNVs in fetuses with MCDK was 16.7% by CMA. 17q12 microdeletion syndrome and WBS are associated with MCDK. Mutations of PEX26, FKBP6, TUBGCP6, ALG12, and CYP4A11 genes may be the causes for MCDK.
Adult ; Chromosomes ; genetics ; Female ; Fetus ; Humans ; Male ; Microarray Analysis ; methods ; Multicystic Dysplastic Kidney ; genetics ; Pregnancy ; Prenatal Diagnosis ; methods ; Young Adult
8.Antimicrobial resistance surveillance of gram-positive cocci isolated from 14 hospitals in China in 2011
Yu GUO ; Hui WANG ; Chunjiang ZHAO ; Zhanwei WANG ; Feifei ZHANG ; Bin CAO ; Bijie HU ; Kang LIAO ; Yaning MEI ; Qing YANG ; Yingchun XU ; Minjun CHEN ; Zhidong HU ; Ziyong SUN ; Liyan ZHANG ; Yunsong YU ; Yunzhuo CHU ; Xiuli XU ; Yuxing NI
Chinese Journal of Laboratory Medicine 2012;(11):1021-1028
Objective To investigate antimicrobial resistance among gram-positive cocci in 14 teaching hospitals in China in 2011.Methods From June 2011 to December 2011,1498 consecutive and non-repetitive gram-positive cocci were collected from 14 teaching hospitals.The minimal inhibitory concentration (MIC) of antibacterial agents was determined by agar dilution method.A retrospective study was conducted on rates of resistance to antimicrobial agents.Data was compared using chi-square test.Results The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) and methicillinresistant coagulase-negative Staphylococci (MRCoNS) was 43.7% (222/508),and 85.6% (214/250),respectively.The MRSA prevalence ranged from 20.0% to 63.5% in different regions.About 58.2% (82/141) of Staphylococcus aureus from respiratory tract specimens,44.8% (48/107) of Staphylococcus aureus from blood samples,and 23.8% (31/130) of Staphylococcus aureus from pus and wound were resistant to methicillin.The susceptible rates of MRSA to chloramphenicol and sulfamethoxazole-trimethoprim SXT were 94.1% (209/222) and 83.3% (185/222),respectively.Susceptibility to gentamycin,erythromycin,clindamycin,tetracyclines,rifampicin and quinolones were from 11.3% to 52.3%.All Staphylococci isolates were susceptible to vancomycin,teicoplanin,linezolid and daptomycin.Five vancomycin-resistant enterococcus (VRE) strains were found in this study.All enterococcus isolates were susceptible to daptomycin(268/268),and 98.3% (118/120) of E.faecalis and 99.3% (147/148) of E.faecium were susceptible to linezoild.About 45.9% (68/148) of E.faecalis and 67.5% (81/120) of E.faecium were resistant to high concentration gentamycin.The susceptibility of E.faecalis to all the antibiotics except for chloramphenicol and tetracycline was higher than that of E.faecium.The prevalence of penicillinnonsusceptible Streptococcus pneumoniae (PNSSP) was 15.5% (37/239).The prevalence of PNSSP in children below 3 years-old was 25% (13/52),and the prevalence of PNSSP from other patients was 13%(24/187).About 91.6% (219/239),88.7% (212/239) and 88.3% (211/239) of S.pneumonia was resistant to erythromycin,clindamycin and tetracyclines.All S.pneumoniae strains were susceptible to teicoplanin,vancomycin,linezolid,tigecycline and daptomycin.Penicillin still showed high activity against Streptococcus spp.β-hemolytic group.More than 60% of Streptococcus.spp.β-hemolytic group are resistant to erythromycin,clindamycin and tetracyclines.Conclusions Based on regions,the resistance rates of Gram-positive cocci are different,of which,the increasing tendency should be taken seriously.Teicoplanin,vancomycin,linezolid,tigecycline and daptomycin show very high activity against Gram-positive cocci.
9.Report from Chinese Meropenem Susceptibility Surveillance in 2010 : antimicrobial resistance among nosocomial gram-negative bacilli
Hui WANG ; Chunjiang ZHAO ; Zhanwei WANG ; Yuxing NI ; Minjun CHEN ; Yingchun XU ; Yunsong YU ; Liyan ZHANG ; Yaning MEI ; Yunzhuo CHU ; Zhidong HU ; Kang LIAO ; Xiaobing ZHANG ; Ziyong SUN ; Xiuli XU ; Xinhong HUANG ; Feifei ZHANG ; Jixia ZHANG ; Qi WANG
Chinese Journal of Laboratory Medicine 2011;34(10):897-904
Objective To investigate antimicrobial resistance among gram-negative bacilli in China in 2010.Methods A total of 1 259 consecutive and non-repetitive gram-negative bacilli were isolated from 13 teaching hospitals from September to December in 2010 in China.All of these isolates were sent to the central laboratory for re-identification and susceptibility testing.The MIC of meropenem and other antibacterial agents were determined by agar dilution method.Interpretive results was determined by CLSI M100-S21.Results The activity of 14 antibacterial agents against 845 Enterobacteriaceae isolates was as follows in order:meropenem ( 98.1%,829 ),amikacin ( 94.0%,794 ),imipeuem ( 90.0%,761 ),piperacillin/tazobactam ( 87.5%,739 ),cefepime ( 83.0%,701 ),ertapenem ( 82.4%,696 ),cefoperazone/sulbactam ( 80.3%,678 ),colistin (75.4%,637),ceftazidime (70.0%,591 ),ciprofloxacin (59.1%,499 ),cefoxitin ( 54.8%,463 ),ceftriaxone ( 53.5%,452 ),cefotaxime ( 52.3%,442 ) and minocycline(51.5%,435).The prevalence of ESBL was 61.3% (106/173) in Escherichia coli,which was much higher than 41.2% (70/170) in Klebsiella pneumoniae.The susceptibility rate of Klebsiella pneumoniae against meropenem,imipenem,amikacin and colistin were more than 90%,but were highly resistant to ceftriaxone and cefotaxime.Over 80% of Enterobacter cloacae,Enterobacter aerogenes,Citrobacter freundii,were susceptible to meropenem,amikacin,cefepine,cefoperazone/sulbactam,imipenem,piperacillin/ tazobactam,and ertapenem.The most active antibiotics against Pseudomonas aeruginosa were Colistin (98.4%,182),Amikacin ( 85.9%,159 ),Piperacillin/Tazobactam ( 80%,148 ),Ceftazidime ( 79.5%,147),Meropenem (74.1%,137),Ciprofloxacin (74.1%,137),Cefepime (73.5%,136),Imipenem (71.9%,132) and Cefoperazone/Sulbactam (70.8%,131 ).Less than 37% of Acinetobacter baumannii isolates were resistant to carbapenems.The susceptible rate to Minocycline was 47.8%.Colistin kept good activity against Acinetobacter baumannii (susceptible rate,97.8%,n =176),The prevalence of Pan-drug resistant P.aeruginosa and A.baumannii was 18.9% (n =35),and 61.8% (n =108),respectively.Conclusions Carbapenems remained very high activity against Enterobacteriaceae.Increasing resistance to the antimicrobials agents test among A.baumanni and P.aeruginosa,especially carbapenems among A.baumanni brought great concern.
10.Antimicrobial resistance surveillance of gram-positive cocci isolated from 15 teaching hospitals in China in 2013
Yu GUO ; Hui WANG ; Chunjiang ZHAO ; Feifei ZHANG ; Zhanwei WANG ; Bin CAO ; Yingchun XU ; Minjun CHEN ; Bijie HU ; Yuxing NI ; Liyan ZHANG ; Kang LIAO ; Qing YANG ; Yunsong YU ; Xiuli XU ; Yunzhuo CHU ; Zhidong HU ; Ziyong SUN ; Yaning MEI ; Zhiyong LIU
Chinese Journal of Laboratory Medicine 2015;(6):373-381
Objective Toinvestigateantimicrobialresistanceamonggram-positivecocciinChinain 2013.Methods Retrospectivestudy.FromJune2013toDecember2013,1663consecutiveandnon-repetitive gram-positive cocci were collected from 15 teaching hospitals. The minimal inhibitory concentration ( MIC) of antibacterial agents was determined by agar dilution method. A retrospective study was conducted on rates of resistance to antimicrobial agents. The prevalence of penicillin-resistant Streptococcus pneumoniae ( PRSP) between children and adult patients and the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) between elder group and younger adult patients were compared using chi-square test. Results The prevalence of PRSP in children below 3 years old ( 72. 9%, 51/70 ) was higher than adult patients (55. 2%, 106/192) (χ2 =6. 653,P<0. 05). About 94. 9%(261/275) and (92. 7%,255/275) of S. pneumonia were resistant to erythromycin and clindamycin. All S. pneumoniae strains were susceptible to teicoplanin, vancomycin, linezolid, tigecycline and daptomycin. Penicillin still showed very high activity against Streptococcus spp. β-Hemolytic group. More than 60% of Streptococcus spp.β-Hemolytic group were resistant to erythromycin, clindamycin and tetracyclines. The prevalence of MRSA and methicillin-resistant coagulase-negative Staphylococci(MRCoNS) was 39. 7%(229/576) and 80. 6%(224/278), respectively. The MRSA prevalence ranged from 24. 2% to 70. 0% in different regions. About 52. 6%( 100/190 ) of Staphylococcus aureus from respiratory tract specimens, 38. 5%(40/104)of Staphylococcus aureus from blood samples, and 29. 7%(58/195) of Staphylococcus aureus from wound and pus were resistant to methicillin. The prevalence of MRSA in elder group ( 48. 6%, 84/173 ) was higher than that in younger adult patients (35. 7%, 144/403)(χ2 =8. 322,P <0. 05). The susceptibility rates of MRSA to chloramphenicol and trimethoprim/sulfamethoxazole were 86. 4% ( 244/228 ) and 94. 7% ( 237/228 ) , respectively. Susceptibility rates to gentamycin, erythromycin, clindamycin, tetracyclines, rifampicin and quinolones were ranged from 15. 8% to 59. 6%. All Staphylococci isolates were susceptible to teicoplanin, vancomycin, linezolid, daptomycin and tigecycline. All Enterococcus isolates were susceptible to daptomycin and tigecycline. All E. faecalis ( 158/158 ) and 96. 4% ( 133/138 ) of E. faecium were susceptible to teicoplanin. About 98. 0% ( 150/153 ) of E. faecalis and 97. 1% ( 145/138 ) of E. faecium were susceptible to linezoild. About 45. 8% (70/153) of E. faecalis and 60. 9% (84/138) of E. faecium were resistant to gentamycin with a high concentration. The susceptibility of E. faecalis to all the antibiotics tested exceptchloramphenicolandtetracyclinewashigherthanthatofE.faecium.Conclusions Basedon different age groups and regions, the resistance rates of Gram-positive cocci are different. Teicoplanin, vancomycin, tigecycline, daptomycin, linezolid and tedizolid showed very high activity against Gram-positive cocci. (Chin J Lab Med,2015,38:373-381)