Objective:To investigate the relationship between the level of plasma ammonia(NH3),and the changes of serum alpha-fetoprotein(AFP),cholinesterase(ChE),liver fibrosis index in liver cirrhosis patients with hepatocellular carcinoma(LC-HCC)in reference to Child-pugh grades.Methods: A total of 58 patients with LC and HCC(LC-HCC group),76 patients with LC(LC group)and 60 cases of healthy volunteers(HC group)were collected.Serum AFP were detected by electrochemiluminescence,serum Hyaluronic acid(HA),laminin(LN),type Ⅳ collagen(Ⅳ-C) were detected by chemilu minescence immunoassay,serum ChE were detected by S-Butyrylthiocholine iodide,NH3 was continuously monitored.The levels of AFP,ChE,NH3 and liver fibrosis indexes(HA,LN,Ⅳ-C) among the LC-HCC group,LC group and HC group,different Child-Pugh grades in LC-HCC group were compared.Results: The serum levels of NH3,AFP,HA,LN,Ⅳ-C in LC-HCC group were significantly higher than that in LC and HC group,and ChE levels were significantly reduced,differences between LC group and HC group were statistically significant(P<0.05).With the increase of Child-Pugh grades,the serum levels of NH3,AFP,HA,LN,Ⅳ-C in LC-HCC group were gradually increased but the ChE gradually decreased,the differences were statistically significant(P<0.05).Serum levels of ChE in patients with LC-HCC were significant negatively correlated with Child-Pugh grades,and AFP,NH3,HA,LN,Ⅳ-C were significant positive correlated with Child-Pugh grades(P<0.05).Serum levels of ChE in patients with LC-HCC were significant negatively correlated with LN,Ⅳ-C and HA,while the levels of AFP,NH3 were significant positive correlated with LN,Ⅳ-C and HA(P<0.05).Conclusion: The serum levels of AFP,ChE and NH3 in patients with LC and HCC are significant abnormalities;they are significant positive correlated with Child-Pugh grades;the detection of the these indicators is valuable to non-invasive diagnosis for patients with LC and HCC.

Objective:To investigate the relationship between serum complement C 3,C4 and hepatitis grade level and liver fi-brosis degree in patients with chronic hepatitis B ( CHB ) .Methods: 185 cases of patients with CHB admitted in our hospital from March 2014 to 2016 March were collected as CHB group ,60 cases who were considered healthy at the same period as the control group.The serum complement C3,C4 of the two groups were detected and compared ,and relationship between serum complement C 3, C4 and hepatitis grade level and liver fibrosis degree was analyzed .Results:The serum C3,C4 levels in CHB group were significantly lower than the control group (t=10.476,9.172,P<0.05).The differences of serum C3,C4 levels among different levels of hepatitis activity were significant ( F=4.321 ,3.495 ,P<0.05 ) ,and differences among different levels of hepatic fibrosis grading were significant (F=3.861,4.541,P<0.05).The serum C3,C4 levels were negatively associated with G rating of hepatitis activity (r=-0.575,-0.542,P<0.05),and were negatively associated with S rating (≥S1) of liver fibrosis (r=-0.512,-0.636,P<0.05).Conclusion:The serum complement C3,C4 levels is closely related with hepatitis grade level and liver fibrosis degree in patients with CHB ,it can be used as secondary predictors of hepatic pathological status in CHB patients .

Objective:To investigate the clinical value of the changes of serum complement,immunoglobulin and inflammatory cytokines in children with mycoplasma pneumonia (MP).Methods:60 cases of children with MP infection were collected as MPP group,60 cases of healthy children as control group.The serum levels of complement (C3,C4) and immunoglobulin (IgA,IgG,IgM) were detected by INA,the serum levels of inflammatory cytokines (IL-8,IL-10,IL-13,TNF-α) were detected by ELISA.Results:①The serum levels of IgM,C3,C4,IL-8,TNF-et and IL-13 in MPP group in acute stage were significantly higher than those in the recovery stage and control group (P＜0.05),the levels of IL-8,TNF-et,IL-13 in the recovery stage were significantly higher than those in control group (P＜0.05);the serum levels of IgA,IL-10 in MPP group in acute stage were significantly lower than those in the recovery stage and control group,and these were still significantly lower than the control group (P＜0.05);the IgG in MPP group in acute stage was not significant difference with control group (P＞0.05),but it in recovery stage was significantly higher than the acute stage and the control group (P＜0.05).②The serum levels of IgM,IgG,C3,C4,IL-8,TNF-et and IL-I 3 in MPP group in severe were significantly higher than those in the mild stage and control group (P＜0.05),and the levels of IgM,IL-8,TNF-αt,IL-13 in the severe stage were significantly higher than those in control group (P＜0.05);the serum levels of IgA,IL-10 in MPP group in severe stage were significantly lower than those in the mild stage and control group,and these in mild stage were still significantly lower than the control group (P＜ 0.05).Conclusion:Children with MP infection have a significant cellular and humoral immune dysfunction,the detection of the changes of serum complement,immunoglobulin and inflammatory cytokines is valuable to assessing the disease staging and degree.

Objective To investigate the effects of PTD4-GFP-Apoptin protein on proliferation inhibition and apoptosis-inducing of different types of leukemia cells. Methods Genetic engineering was used to restructure a carrier containing PTD4-GFP-Apoptin gene, and MTT was applied to detect the expressed PTD4-GFP-Apoptin fusion protein and its effect on the leukemia cell proliferation. Flow cytometry (FCM) was used to detect the effects on cell apoptosis. Results MTT cell proliferation inhibitory experiment showed that PTD4-GFP-Apoptin had different degree of proliferation inhibition on different types of leukemia cells;furthermore, the inhibitory effect presented positive correlation with time and concentration. FCM showed that PTD4-GFP-Apoptin had apoptosis-inducing effect on HL-60 cells, and the apoptotic rate had significant difference compared with the control group (P <0.05). Conclusions PTD4 can carry large proteins to penetrate the cell membrane, and PTD4-GFP-Apoptin may produce the inhibiting proliferation in vitro for a variety of leukemia cells. Apoptin can induce tumor cell apoptosis without affecting normal cells, which might become a new agent for the clinical treatment of leukemia.

Objective To investigate the effect of fatty liver graft on early poor prognosis in postoperative liver transplant adult patients.Methods The clinical data of 125 adult patients undergoing liver transplantation (LT) from fatty liver graft from January 2008 to October 2012 were retrospectively analyzed.Patients were divided into poor-prognosis group and non-poor-prognosis group.18 clinical factors were compared between these two groups by single factor and multiple factor Logistic regression analysis.Results Degree of steatosis (x2 =10.088,P =0.004),recipients' age (t =-3.917,P =0.002),pre-LTserum creatinine values (Z =-2.623,P =0.009),liver warm ischemia time (Z =-2.305,P =0.021),cold ischemia time (Z =-3.394,P =0.001) were identified to be statistically significant by the single factor analysis (P ＜ 0.05);By multivariate stepwise Logistic regression analysis on the above parameters,cold ischemia time (x2 =10.141,P =0.001,OR =1.003) and degree of steatosis (x2 =8.360,P =0.004,OR =4.619) were found as the independent risk factors,into the regression equation:Y =0.003 × cold ischemia time + 1.530 × degree of steatosis-4.243.Conclusions Donor liver cold ischemia time,degree of steatosis are independent risk factors for poor prognosis in early stage after liver transplantation.

ObjectiveTo investigate the effects of different laboratory test reagents for hepatitis C virus (HCV) antibody through a comparative analysis. MethodsA total of 207 samples which tested positive by four anti-HCV screening reagents commonly used in the laboratories in China (Kehua, Xinchuang, Wantai, and Abbott) were included. HCV RNA nucleic acid amplification (NAT) was performed, and if NAT results were negative, recombinant immunoblot assay (RIBA) was performed for further confirmation. The test results of these four screening reagents were compared, and their S/CO values and true positive rates were analyzed. ResultsOf all the 205 samples testing positive by any one reagent, 191 (93.2%) tested positive by the four reagents, and 14 (6.8%) were tested inconsistently by the four reagents. The positive predictive values of Xinchuang, Kehua, Wantai, and Abbott reagents were 88.2% (180/204), 93.8% (180/192), 91.4% (180/197), and 90.0% (180/200), respectively. The S/CO thresholds with a positive predictive value of ≥95% for Xinchuang, Kehua, Wantai, and Abbott reagents were 9.0, 4.0, 5.0, and 7.0, respectively. ConclusionXinchuang, Kehua, Wantai, and Abbott reagents have significantly different S/CO thresholds with a positive predictive value of ≥95%, which are significantly different from those in other domestic laboratories. Each laboratory should establish an applicable S/CO threshold with a positive predictive value of ≥95%, in order to reduce the sample size for confirmatory test.