Antineoplastic Agents ; adverse effects ; Humans ; Integrative Medicine ; Organoplatinum Compounds ; adverse effects ; Peripheral Nervous System Diseases ; chemically induced

Objective To study the factors such as artificial abortion frequency, pregnancy time and so on on development of adeno-myosis (AM). Methods A retrospective analysis was carried in 462 patients who were from the department of gynaecology and obstetrics in Affiliated Hospital of Nantong University and Beijing Aviation Center Hospital from January 2004 to December 2008. The patients were divided into two groups: AM and no - AM. The two groups were compared for abortion frequency, pregnancy time, age, menarche age and so on. Results There were no significant differences between the two groups in age, menarche age, age of the first artificial abor-tion, bearing frequency, IUD with pregnancy and so on(P > 0.05). The proportion of patients with repeated artificial abortion or history of curettage of uterus in AM group was higher than that in the no - AM group. The patients with pregnaney time more than 8 weeks who re-ceived artificial abortion had higher rate of development of AM. Conclusion Repeated artificial abortion, pregnancy time and history of curettage of uterus were the risk factors of development of adenomyosis.

Objective To investigate the water source pollution by parasitic protozoa in small towns and villages in Shenzhen,China,in order to present the data to establish the national hygienic standard for controlling parasitic protozoa pollution.Methods The samples of water source were collected from 8 village level water plants in Shenzhen.Giardia lamblia and Cryptosporidium were detected,according to the EPA methods,by filtering,rinsing,magnetic isolation and staining.Results Giardia lamblia was found in water sources of 6 water plants and Cryptosporidium was found in one water plant.Conclusion At present,some water sources of village level water plants where the reservoirs were used as the water source in Shenzhen have been polluted by Giardia lamblia and Cryptosporidium,that indicates the potentiality of the water-born-diseases in that areas.

Objective To explore the value of dynamic contrast-enhanced CT for differentially diagnosing benign and malignant the solitary pulmonary nodules. Methods 117 solitary pulmonary nodules proved by histopathological examination were enrolled in this study. Thin-section CT scanning was performed before and 1,2,3,4 and 5 minutes after contrast enhancement. Results The enhanced value of malignant nodules (39.6?15.8)Hu was significantly higher than that of benign nodules (19.18?17.23)Hu (P

0.05).The proportion of patients with repeated artificial abortion or history of curettage of uterus in AM group was higher than that in the no-AM group.The patients with pregnancy time more than 8 weeks who received artificial abortion had higher rate of development of AM.Conclusion Repeated artificial abortion,pregnancy time and history of curettage of uterus were the risk factors of development of adenomyosis.

Objective To study and design a new algorithm of QT interval measurement based on multi-scale morphological derivative transform(MMDT) according to the demands of mobile monitoring platform.Methods After the MMDT of ECG signals by inducing a triangle and a short line as a pair of structure elements,they were classified into four types by their morphological characteristics and the optimal strategy for each type was described to detect the onset of Q-wave.By introducing the "wing" function,two referenced points of T peak were picked out,which was helpful to locate the peak and offset of T wave,and improve the precision and recognition rate of MMDT method when detecting the bifid T wave(90.9%)and biphasic T wave(86.7%).Results Eighty data records from CSE database were used to evaluate the availability.By contrast with the wavelet transform method,the statistical results showed that the proposed algorithm had generally less error and smaller standard deviation especially for abnormal-phase T wave.Conclusion Compared with those algorithms based on wavelet transform and self-adaptive threshold techniques,our algorithm needs less empirical parameters and calculation.It is also suitable for mobile monitoring and HOLTER system,and has a wide prospect of application.

Objective To estimate reliability of magnetic resonance elastography (MRE) in measuring liver stiffness of volunteers and patients with chronic liver disease and to assess inter-rater consistency.Methods MRE was performed on a 3.0 T scanner in all subjects,including 24 volunteers (control group) and 64 patients with liver disease (chronic liver disease group).Liver stiffness was measured blindly by two raters.The pathological fibrosis score was applied as a standard reference for liver fibrosis in 22 patients.The intraclass correlation coefficient (ICC) was used to evaluate inter-rater reliability.The differences of liver stiffness between two groups were evaluated using non-parametric MannWhitney U test.Spearman analysis was used to analyze the correlation between fibrosis stages and liver stiffness.Results The intraclass correlation coefficient of liver stiffness was perfect (ICC =0.99,P ＜ 0.01)between two raters.There was significant difference of mean stiffness between control group and patient group (U =90.5,P ＜0.01) with(2.35 ±0.34) kPa and(4.17 ± 0.47) kPa,respectively.The correlation between fibrosis stage (3,3,5,5 and 6 patients in fibrosis stage S0,S1,S2,S3 and S4) and stiffness (2.13,3.25,3.82,5.45 and 7.35 kPa) was very strong (r =0.96,P ＜0.01).Conclusion MRE is a reliable and promising tool to measure liver stiffness and to assess liver fibrosis.

Objective To investigate ventricular remodeling and glucose transporter 4(GLUT4) expression in rat with type 2 diabetes mellitus(DM) complicated with myocardial infarction (MI), as well as the effects of ramipril on ventricular remodeling and CLUT4 expression. Method Diabetic animal model (DM) was produced by cholesterol-diet and intraperitoneal injedtion of streptozotocin (STZ,30 mg/kg). The successful model was identified based on the blood gulcose value > 6.7 mmol/L. Left anterior descending coronary artery was ligated to produce the model of MI. 46 wistar rats were randomly(random number) divided into 5 groups: DM + MI + placebo group(placebo group, n = 9), DM + MI + ramipril group(ramipril group, n = 10), DM + sham-operation group (DM group, n = 10),non-DM group + MI (MI group, n = 9), and non-DM + sham-operation group (n = 8). After six weeks, echocardiographic studies was performed ; Left ventricular mass index (LVMI), myocardial infarct size and myocardial collagen volume fraction(CVF) of non-infarction area were also detected. GLUT4 mRNA in the myocardium away from infarction region was measured with real-time fluorescent quantitative RT-PCR and the expression of GLUT4 protein was measured with western blot. Analysis of variance was used to compare the groups. P -values< 0.05 were considered to indicate statistically significant differences. Results Compared with Ml group and DM group,EF[(32 ±3)% vs. (65 ±4)%, P <0.01 and (32 ±3)% vs. (38 ±4)%, P <0.05 respectively] significantly decreased,while E/A ratio (P < 0.01), LVMI (P < 0.05) and CVF (P < 0.05) obviously increased in placebo group. The expression of GLUT4 mRNA and protein were similar between placebo group and DM group, however, much lower than those in MI group(P<0.01).In rampiril group, E/A ratio (P < 0.01), LVMI(3.1±0.2 vs.4.2±0.4, P <0.01) and CVF(P < 0.05) significantly decreased compared with placebo group, while EF[(45±3)% vs.(32±3)%, P <0.01] and the expression of GLUT4 mRNA and protein (P < 0.05) increased. Conclusions Rampiril could improve left ventricular hypertrophy and systolic/di-astolic dysfunction, increase the expression of GLUT4 mRNA and protein in diabetic rats complicated with MI.

Objective To evaluate arthroscopic PCL (posterior cruciate ligament) reconstruction with an implant fixaliun of hamstring tendon knot and bottle-neck technique.Methods Thirteen cases with PCL injury were treated with arthroscopic PCL reconstruction with an implant fixation of hamstring tendon knot and bottle-neck technique between April,2002 to June,2005.The patients were followed up fur at least seven months to evaluate the clinical effects by means of ROM of the joint,post drawer test,Lachman test,Lysholm and IKDC (International Knee Documents Committee) scores.Results The patients were followed up for an average of 13.4 months.No limitation of knee extension was found six months after operation.Eleven patients had normal knee flexion(＞140?), one had knee flexion of 120?and one 100?.In posterior drawer test,11 patients were gradeⅠand two gradeⅡ.In Lachman test,11 patients were rated as within gradeⅠand two gradeⅡ.By Lysholm knee function scale,the average knee scores were 90.47?4.13 and 78.82?2.46 respectively before and after operation,and the dif- ference was of statistical significance (t=2.416,P=0.029).By IKDC scale,six were grade A,five grade B and one grade C.Conclusion Arthroseopic PCL reconstruction with an implant fixation of hamstring tendon knot and bottle-neck technique is a reliable method to restore knee function and will have a prospect of wide application be- cause of its good tissue biocompatibility,less invasion,and less operative expenditure.

Objective To study the expression of urokinase-type plasminogen activator (uPA) and its relation with expression of receptor (urokinase-type plasminogen activator receptor uPAR) in epithelial ovari- an cancer (OEC) and with the clinic prognosis.Methods Expression of uPA and uPAR protein was detected by Streptavidin-biotin-HRP in 68 cases of epithelial ovarian cancer and compared with that in 10 cases of borderline tumor,10 cases of benign tumor and 10 cases of normal tissue,and correlation between them was analyzed.The different expression groups of uPA was correlated with the prognosis of ovarian epithelial can- cer.The expression of uPA showed a correlation with short survival time (P