Objective To observe the effects of dexmedetomidine on serum TNF-αand IL-6 in neurosurgical patients during perioperative period. Methods 60 cases patients with intracranial aneurysm treated in our hospital in January 2014 to August 2014 were selected as the research objects, and they were randomly divided into two groups with 30 cases in each group.Before induction, the observation group adopted 1μg/kg dexmedetomidine with 10 min infusion, then adopted 0.4μg/( kg? h) infusion of dexmedetomidine, and control group received saline infusion.Serum TNF-αand IL-6 levels in two groups were compared at different time.Results After application of dexmedetomidine, the related indexes of hemodynamics in observation group, mean arterial pressure (MAP) and heart rate (HR) were more stable (P<0.05).Serum levels of TNF-αat the time of after induction, skin incision operation, 1h operation in observation group were (1.32 ±0.35), (1.42 ±0.54), (1.52 ±0.67), (1.57 ±0.63) μg/L, and were lower than those of control group, respectively (P<0.05).Serum levels of IL-6 at the time of after induction, skin incision operation, 1h operation in observation group were (69.64 ±13.54), (72.43 ±16.75), (76.32 ±16.93), (77.42 ±15.75) μg/L and were lower than those of control group, respectively (P<0.05).Conclusion The hemodynamics after application of dexmedetomidine in patients with neurosurgery operation is more stable, and the serum TNF-αand IL-6 are lower, so it contributes to the anti-inflammatory and has important clinical value.

OBJECTIVE:To observe the cerebral protective effects of dexmedetomidine in brain aneurysm surgery. METH-ODS:48 patients with intracranial aneurysm were randomly divided into observation group and control group,with 24 cases in each group. Observation group was given dexmedetomidine 1 μg/kg,pump time 10 min,at rate of 0.5 μg/kg;control group re-ceived constant volume of normal saline. Hemodynamic changes,the levels of S100β protein and neuron specific enolase (NSE) were compared between 2 groups. RESULTS:The heart rate and mean arterial blood pressure were more stable in the observation group after medication,with statistical significance(P<0.05). The serum level of S100β protein at the end of operation(T5),6 h after operation(T6),12 h after operation(T7),24 h after operation(T8)were(1.52±0.35)μg/L,(1.69±0.33)μg/L,(1.72±0.42)μg/L,(2.08±0.57)μg/L;the serum level of NSE were(10.9±1.2)ng/L,(13.5±1.5)ng/L,(16.8±2.8)ng/L and(19.7±4.3)ng/L in observation group;those were all lower than in control group,with statistical significance(P<0.05). CONCLUSIONS:Dexme-detomidine for cerebral aneurysm operation is helpful for hemodynamics stability,and result in lower S100β protein and NSE. So it is important for cerebral protection.

Objective To evaluate cortisol level in experimental frequent febrile seizures model rats.Methods Rats were divided into 3 groups: normal control group(n=10,NC group),hyperthermic control group(n=12,HC group) and febrile seizures group(n=21,FS group).Cortisol level in serum and hippocampus of rats were determined by ELISA.Ultrastructural changes of neurons in hippocampal region CA1 was observed by electron microscop.Results 1.No special ultrastructual findings were observed from neurons in hippocampal CA1 in NC and HC group.In FS group,the neuronal degeneration and edema,the mitochondrial degeneration and edema,the mitochondrion ridge obscured or disappeared.2.Cortisol levels in serum and hippocampus in FS group [(26.57 ? 15.94)?g/L and((4.11)?1.74)?g/L,respectively] were significantly lower than those in HC group [(40.04 ? 15.72)?g/L and(6.70?3.45)?g/L,respectively](P0.05).Positive correlations were demonstrated between serum cortisol level and hippocampus cortisol level(r=0.40 P

0.05).Conclusions Frequent FS might not change the expression levels of IL-6 and TNF-? in hippocampus.IL-6 and TNF-? might not play a role in development of FS induced brain damage.

The concentration of 1.8 ng/ml platelet activating factor (PAF) caused the elevation of pulmonary vascular permeability and a steady increase of lung weight gain (LWG) in isolated perfused rat lung. The LWG, fluid filtration coefficient and extravasation amount of T-1824 labelled albumin were lower of 89. 2%, 70. 9% and 75. 5%, respectively, in PAF perfusion without Ca2+ group than those in PAF perfusion with Ca2+ group, within 35 min. Mean pulmonary capillary pressure and pulmonary arterial pressure decreased from + 0.725 ? 0.389 kPa and +1.187 ? 0.320 kPa to -0.708 ? 0.315 kPa and - 0.025 ? 0.279 kPa, respectively. Calcium free perfusate without PAF slightly increased vascular permeability in isolated rat lungs. The results suggest that PAF-induced rat lung injury depends on the existence of extracellular calcium ion.

A bolus injection of endotoxin and platelet activating factor ( PAF ) via external jugular vein in rats resulted in severe pulmonary edema, accompanied by significant increase in extravascular lung water volume, bronchoalveolar lavage fluid ( BALF ) protein concentration, BALF cell counts and polymorphonuclearcyte percentage. PAF receptor antagonist SRI63-441 showed partial protective effects on endotoxin and PAF induced lung edema and BALF cytological changes.

The lecture, role play, combination of team learning and self-directed learning, group presentation,these multiple pedagogies were adopted in the teaching of selective medical English course.The questionnaire result showed this multiple teaching strategy can foster the students' interest in medical English, enhance their awareness of active learning and increase the ir learning efficacy, which is an effective teaching model, and can be applied and promoted in the medical English teaching.

OBJECTIVETo explore the effect of Danlou Tablet (DT) on arrhythmia model rats induced by transient myocardial ischemia/reperfusion (I/R).

METHODSTotally 45 healthy Wistar rats were randomly divided into 3 groups, the sham-operation group, the model group, and the DT group, 15 in each group. Rats in the sham-operation group and the model group were administered with distilled water by gastrogavage at the daily dose of 0.1 mL/kg. Rats in the DT group was administered with 0.53 g/mL DT suspension by gastrogavage at the daily dose of 0.1 mL/kg. All medication was lasted for 10 successive days. The myocardial I/R experiment was performed at 1 h after the last gastrogavage. ECG was performed before ligation and at I/R. The jugular arterial blood pressure of all rats was measured during the whole course. ST segment changes were observed at each time point of I/R. The ventricular fibrillation, the premature ventricular, the number and the duration of ventricular tachycardia within 30 min reperfusion were also observed. Activities of Na(+)-K+ ATPase and Ca2+ ATPase in the myocardium homogenate were detected as well.

RESULTSThe jugular arterial blood pressure and the heart rate were slightly lower in the DT group than in the model group, but with no statistical difference (P > 0.05). Compared with the sham-operation group, the degree of ST segment was obviously elevated in the model group at 0, 5, and 7 min (P < 0.05). It was significantly lower in the DT group than in the model group (P < 0.01). ST seg ment was more elevated at 5 min than at 0 min in the model group, but the degree of ST segment elevation was still obviously lower in the DT group than in the model group (P < 0.05). There was no statistical difference in the degree of ST segment elevation at 7 min between the two groups (P > 0.05). At 0 min when the decrement of ST segment exceeded one half the ischemia, there was no statistical difference in the degree of myocardial ischemia between the model group and the DT group (P > 0.05). Compared with the model group, the incidence of fatal and nonfatal ventricular fibrillation, the frequency and duration of ventricular tachycardia and premature ventricular beats were obviously lessened, and activities of Na(+)-K+ ATPase and Ca(2+)-ATPase increased (all P < 0.05).

CONCLUSIONSDT could significantly protect arrhythmias induced by transient I/R. Its effect might be related to lowering the degree of myocardial ischemia, and increasing ion transport channel related enzyme activities.

Animals ; Arrhythmias, Cardiac ; drug therapy ; etiology ; Disease Models, Animal ; Drugs, Chinese Herbal ; therapeutic use ; Male ; Myocardial Reperfusion Injury ; complications ; Rats ; Rats, Wistar

Humans ; Pediatrics ; manpower ; Personnel Administration, Hospital ; Respiratory Therapy Department, Hospital ; manpower

Objective To explore the expression and significance of hedgehog signal molecules (Ptch, Smo and Gli1 ) in pancreatic cancer. Methods Two hundred SD rats were randomly divided into DMBA group ( group A, n = 90), cyclopamine intervening group ( group B, n = 90) and control group ( group C, n = 20).For group A and B, DMBA was directly implanted into the parenchyma of the pancreas to establish the model of pancreatic cancer. The rats in group B were treated with 6.25 ml/kg cyclopamine and DMSO solution intraperitoneally daily. All rats were sacrificed four months later to observe the pancreatic tissue pathologic changes, and immunohistochemistry SP was used to detect the expression of Ptch, Smo, Gli1 protein in pancreatic cancer and normal pancreatic tissue. Results The prevalence rate of pancreatic cancer in group A was 57.5％ (46/80), the maximum size of the tumor was 0.5 ～ ＞2 cm; the prevalence rate of pancreatic cancer in group B was 17.1％ ( 14/82), the maximum size of the tumor was 0.5 ～ 2.0 cm, and the difference between the two group was statistically significant (P ＜0.05). The positive expression rate of Ptch, Smo and Gli1 protein was 82.6％, 73.9％ and 65.2％ in DMBA group, and was 50.0％, 42.9％ and 28.6％ in cyclopamine group, and the difference between the two group was statistically significant ( P ＜ 0.05 ). Ptch,Smo and Gli1 protein was expressed in normal pancreatic tissue. Conclusions Direct implantation of DMBA in the parenchyma of rat pancreas can induce pancreatic cancer with a high incidence in a short time.Hedgehog signal protein expression is significantly increased, cyclopamine can inhibit the occurrence and progression of pancreatic cancer by inhibiting Hedgehog messenger expression.