Objective In order to get genetic markers,an auxotrophic paclitaxel-producing fungus named Pestalotiopsis malicola N8 strain was isolated by genetic modification.Methods Based on the homologous recombination,URA-3 which is the key gene for uracil synthetic route of Pestalotiopsis malicola N8 strain was knocked out.The transformants were screened by minimal medium with the combination of 5-fluoroorotic acid (5-FOA) and uracil.Results The results showed that the uracil auxotrophic strain was able to grow in the minimal medium containing 5-FOA and uracil while the wild type strain was not.Conclusions The uracil auxotrophic strain can be used as a new selection marker for future gene function studies of N8 strain.

Objective To investigate the effect of hyperbaric oxygen on cerebral hemodynamics and intracranial pressure(ICP)in patients with severe traumatic brain injury (SBI).Methods A retrospective analysis was conduccted .82 cases of SBI patients treated in our hospital from January 2013 to January 2014 were randomly and evenly divided into two groups.Control group received conventional treatment while observation group was treated with continuous hyperbaric oxygen therapy.Intracranial hemodynamic parameters before and after treatment were determined and compared between the two groups.Results Peak systolic velocity ( Vs) and mean flow velocity ( Vm) of the middle cerebral artery ( MCA) were faster in hyperbaric oxygen group than in control group (P<0.05); the pulsatility index was lower than in control group (P<0.05);intracranial pressure (ICP) values (11.20 ±3.39)mmHg were significantly lower in observation group after hyperbaric oxygen therapy than in control group (15.24 ±3.13)mmHg.The difference was statistically significant (P<0.05), and the total effective rate was 85.37% vs 68.29% in control group(P<0.05).Conclusion Early hyperbaric oxygen therapy can significantly enhance SBI patients immune function and the ability to fight against infections in critically ill patients, therefore improving patients outcomes and overall quality of life .

Objective:To investigate the effects of the selective ?3-adrenoreceptor (AR) agonist,CL 316243,on the contractions and levels of cAMP and cGMP of myometrial strips isolated from term-pregnant rats.Methods:Salbutamol,an agonist of ?2-AR,was chosen as positive control.We evaluated the effect of increasing concentrations of CL 316243 and salbutamol on oxytocin-induced myometrialcontractions with or without metoprolol,ICI 118.551 and SR 59230A.Effects of CL 316243 and salbutamol on cAMP and cGMP levels in isolated myometrial strips were also evaluated by radioimmunoassay.Results:CL 316243 concentration-dependently decreased the amplitude of oxytocin-induced contractions with Emax value(48.13%?5.61%)ofCL316243,whichwas significantlylargerthanEmaxvalue(35.52%?5.15%)ofsalbutamol(P

With the rapid development of modem medical imaging technology,imaging examination especially CT and MRI plays an increasingly important role in the quantitative analysis of ophthalmic diseases,including the measurement of orbital volume with CT, the morphological changes of extraocular muscles in different eye positions,changes of oxygen pressure of the retina and the breakdown of blood-retinal barrier,the quantitative analysis of fiber bundles of visual pathway and so on.In this paper,a brief review was made about the application of imaging measurement with CT and MRI in ophthalmic diseases.

Although the efficacy of tyrosine kinase inhibitor (TKI) for the treatment of chronic myeloid leukemia (CML) is obvious, the drug resistance is still inevitable, therefore, TKI drug resistance has become one of the reasons for the failure treatment of CML. According to the literature, about 5 % patients have primary resistance to TKI, and 20 %-30 % patients have secondary resistance to TKI. Current TKI drug resistance molecular mechanisms include the over-expression of bcr-abl, gene mutation, defect of DNA repair mechanism, medicine excretion mediated by ATP-binding cassette translocator, abnormal signaling pathway and bone marrow microenvironment. Meanwhile, the occurrence of drugs, based on the drug resistance mechanism development in preclinical or clinic investigation stage, are likely to provide the possibility for the overcoming of TKI drug resistance. This paper will review the progress of molecular mechanism of TKI drug resistance and the therapy strategy after drug resistance.

The international Non-governmental Organizations ( NGOs) have been playing an important role in the field of global health, which also represents a channel for countries' international experience. This research fo-cuses on the role of NGOs in global health, their governance tools, global health program management experience, and current status of development for Chinese NGOs. In light of international experiences, we suggest the Chinese government to support a few top NGOs to engage intensely in global health, build management and cooperation mechanism with NGO which will be considered as important complemertary role. The Chinese government shonld implement “major support and comprehensive improvement” strategy.

OBJECTIVE:To study the effect of five penetration enhancers on the transdermal permeability of sodium phenytoin in vitro.METHODS:Experiments were carried on penetration experimental facility using modified Franz-type horizontal diffusion cell with 0.9% sodium chloride as receptor solution.The content of sodium phenytoin in the receptor solution was determined by HPLC,and its accumulative permeation amounts and steady permeation rate were computed as well.The permeability of sodium phenytoin across the excised rat abdomen skin which has been pretreated with different penetration enhancers-ocenol(3%,5%,and 10%),menthol(3%,5%,and 8%),eucalyptus oil(0.5%,1%,and 3%),azone(1%,3%,and 5%) and N-methyl pyrolidone(3%,5%,and 8%) was observed.RESULTS:Five enhancers did improve the transdermal delivery of sodium phenytoin in a concentration dependent manner.At a respective optimum concentration,the permeation enhancing effects of enhancers in ascending order were 5% N-methyl pyrolidone

OBJECTIVE:To discuss the adjustment of patients'medication psychology in clinical pharmaceutical care.METHODS:Various factors that influence patients'medication psychology and the effects resulted from patients'medication psychology were analyzed.RESULTS&CONCLUSIONS:Patients'psychological effects influence the physiological effects of medicine,thus greatly influencing drug action,therefore,pharmacists'knowledge on pharmacopsychology should be strength?ened and their communication ability with patients should be improved.Furthermore,pharmacists should adjust patients'medication psychology timely to satisfy their basic psychological needs for medication.

Objective:To summarize the participation of clinical pharmacists in the treatment of patients after craniocerebral opera-tion with pulmonary infection. Methods: Clinical pharmacists assisted physicians in identifying infectious bacteria and colonization bacteria of one patient with pulmonary infection after craniocerebral operation and establishing the anti-infection treatment regimen. Re-sults:Clinical pharmacists analyzed various factors such as infection risk factors, clinical and laboratory manifestation and medication history, and effectively selected antibacterial drugs covering the infectious bacteria to timely control the infection. Conclusion: With pharmaceutical knowledge, clinical pharmacists can assist physicians in the interpretation of bacterial culture and drug susceptibility re-ports to promote safer, more timely and effective medication for patients.

Objective There are two main functions of gastrointestinal tract,digestion and absorption,and barrier function.The latter has an important defensive effect,which keeps the body away from the invading and damaging of bacteria and endotoxin.It maintains the systemic homeostasis.Intestinal dysfunction would happen when body suffers from diseases or harmful stimulations.The more serious intestinal disorders would harm the intestinal protective mechanism,or intestinal barrier function,and bacterial/endotoxin translocation,of intestinal failure(IF) would ensue.This article provides a critical review of the evidence indicating that an increase in bacterial translocation is associated with sepsis,and even the multiple organ failure syndrome in critically ill patients.The in-transit microorganisms play an essential role in the homeostasis of local and systemic immunity.Methods All studies published from 2000 to June 2005 about intestinal permeability,bacterial translocation,and systemic inflammatory response syndrome were located by search of PubMed.Results Clinical and experimental studies investigating the correlation between bacterial translocation and systemic inflammatory response syndrome,associated with the damage of the gut barrier function.To keep the mucosal barrier function intact is one of the main issues in the prevention of bacterial translocation.This could be achieved by the adequate delivery of oxygen and nutrient supplementation to the gut.Enteral nutrition,probiotic can be a good choice.Conclusion With a better understanding of the bacteria-host interactions in health and the alterations induced by critical illness,new therapies that improve the environment of both may lead to better recovery rates in intensive care unit patients.