Objective To study the effect of juvenile non-incarcerated oblique inguinal hernia on the development of testis and spermatic cord.Methods The volume of the testis was measured with B-ultrasound in normal and patient children.All patients were surgically treated and the spermatic veins were examined during operation.The patients were followed up for measuring the testicle volume at 3 months,6 months and 6 ～ 12 months after operation.Results Testicle volume on healthy side was larger than the involved side in patient group (P ＜ 0.05).There were still obvious differences between the two sides after 6 months(P ＜ 0.05).No significant difference was found after 6 ～12 months (P ＞ 0.05).Conclusion Non-incarcerated oblique inguinal hernia has effected on the spermatic cord and testicles,which need timely treatment.

OBJECTIVE To investigate the nosocomial infection circumstance in the acute cerebral vascular accident(CVA) in recently two years,and explore the risk factors of nosocomial infection,so that can obtain the methods of controlling the infection.METHODS An restrospective analysis of nosocomial infection circumstance had been accomplished among 656 acute CVA cases from our department ward from Jul 2004 to Jun 2006.RESULTS It was showed cases with nosocomial infection were found among 656 cases.The nosocomial infection rate was 32.67% among inpatients of acute CVA.Nosocomial infection mostly took place within the first 2 weeks after patients hospitalized.Among inpatients with nosocomial infection,the lower respiratory tract infection was the highest(59.15%),the second one was the urinary tract(16.90%).Pathogenic bacteria were mostly the conditional ones.Nosocomial infection was closely related with such factors as the invasive operation,disorder of consciousness,diabetes,age,chronic bronchitis,antibiotic prophylaxis,hospitalization days,and plasma total protein.CONCLUSIONS Nosocomial infection is still a high frequent complication in acute CVA and those related factors should be paid attenion.It is the most important step for the effective control of nosocomial infection and rehabilitation of acute CVA.

Objective To investigate the inhibition effects of different antihistamines on the expressions of chemokines and cytokines released by nasal polyps in vitro.Methods The fresh nasal polyps resected under endoscope from the patients admitted to our hospital were cultured with mizolastine,cetirizine,loratadine,fexofenadine,dexamethasone,or ciclosporin A respectively for 24 h,with the addition of histamine and arachidonic acid.The total RNA of polyps was obtained and purified with tripure isolation reagent.The mRNA expression levels of monocyte chemoattractant protein-1(MCP-1),monocyte chemoattractant protein-3(MCP-3),regulated on activation,normal T cell expressed and secreted(RANTES),eotaxin were analyzed by RT-PCR.The concentrations of interleukin(IL)-4,IL-5 and tumor necrosis factor-?(TNF-?)in cultured supernatant was detected by ELISA.Results The MCP-1 mRNA expression in nasal polyps treated by mizolastine was weaker than those by loratadine and fexofenadine;the MCP-3 mRNA expression by mizolastine was weaker than those by cetirizine and loratadine;the RANTES mRNA expression by mizolastine was weaker than those by loratadine and fexofenadine;the eotaxin mRNA expression by mizolastine was weaker than that by cetirizine(all P

BACKGROUND:Local administration of anti-tuberculosis drugs is a commonly used therapy. Due to the rapid absorption, the drugs cannot have the durable therapy effect; therefore, it is necessary to seek an optimal carrier material for the agents. OBJECTIVE:To review the new development for the carrier materials of anti-tuberculosis drugs. METHODS:A computer-based search of PubMed and VIP databases was performed by the first author to search articles related to sustained-released anti-tuberculosis drugs published from January 1990 to December 2014. The key words were osteoarticular tuberculosis; anti-tuberculosis; sustained-released drugs in English and Chinese, respectively. RESULTS AND CONCLUSION:Inorganic materials (calcium phosphate, calcium sulfate), polymer materials (polylactic acid, polyglycolic acid, polylactic-co-glycolic acid) and biomaterials (protein, glutin, alginates, chitin, demineralized bone matrix) are the main three kinds of carrier materials for anti-tuberculosis drugs. These carrier materials have their own advantages and disadvantages, which cannot be the optimal carrier materials. However, the complex of these materials is a promising technology for the optimal carrier materials in the future.

Objective To investigate the effect and mechanism of probiotic products on alpha-naphthylisothiocyanate(ANIT) induced intrahepatic cholestasis of rats.Methods Seventy-two SD rats were randomly divided into 3 groups: control group(n=8),intoxication group(n=32) and intervention group(n=32).After 3 days adapted feeding,all rats received a single intragastric administration of ANIT(200 mg/kg) to induce acute intrahepatic cholestasis.Probiotics[4.2?108/(kg?d)] was given to intervention group 2 days before ANIT was administrated.After ANIT was given,biliary flow,alanine aminotransferase transaminase(ALT),total bilirubin(TB) and gastrointestinal hormone were recorded in every 48 h till 192 h.SPSS 12.0 software was used to analyze the data.Results 1.Forty-eight hours after ANIT was gavaged,the biliary flow of intoxication and intervention groups were lowered while ALT and TB were increased.But the above observation items of intoxication group were more significantly altered than those of intervention group.Then,they all recovered gradually.Those of intervention group returned to nearly normal levels 144 h after ANIT was gavaged while those of the intoxication group recover at 192 h.2.After ANIT was gavaged,MTL of intoxication and intervention groups was lowered while vasoactive intestinal peptide(VIP) increased.But the above items of intoxication group were more significantly altered than those of intervention group.Then,they all recovered gradually and the intervention group was much more early to returned to normal levels than the intoxication group at 192 h.Conclusions Probiotics can promote the bowel movement through increasing the secretion of gastrointestinal hormone,then relieve the cholestasis.

Adult ; Anti-Bacterial Agents ; adverse effects ; Female ; Humans ; Laryngeal Diseases ; etiology ; microbiology ; Mycosis Fungoides ; etiology ; Prescription Drug Misuse ; Substance-Related Disorders ; complications ; Young Adult

Humans ; MicroRNAs ; Occupational Medicine

Tumors are believed to consist of a heterogeneous population of tumor cells originating from rare cancer stem cells (CSCs). However, emerging evidence suggests that tumor may also originate from non-CSCs. To support this viewpoint, we are here to present definitive evidence indicating that the number of tumorigenic tumor cells is greater than that of CSCs in tumor, and tumor can also derive from non-CSCs. To achieve this, an idealized mathematical model was employed in the present study and theoretical calculation revealed that non-CSCs could initiate the occurrence of tumor if their proliferation potential was adequate. Further, experimental studies demonstrated that 17.7%, 38.6% and 5.2% of tumor cells in murine B16 solid melanoma, H22 hepatoma and Lewis lung carcinoma, respectively, were potentially tumorigenic. Thus, based on the aforementioned findings, we propose that the scarce CSCs, if exist, are not the sole source of a tumor.

AIM:To observe autophagic apoptosis induced by docetaxel in cervical cancer HeLa cells in vitro,and explore its potential molecular mechanism. METHODS:Cervical cancer HeLa cells in vitro were treated with docetaxel of different concentrations (1,5,10,20,40 ?g/mL) and different time (6,12,24,48,72 h). The growth inhibiting of HeLa cells was observed by methyl thiazolyl tetrazolium(MTT) assay. Inverted microscope and electron microscopy were used to observe cell morphological changes. The apoptosis ratio and cell cycle were determined by flow cytometry(FCM). The expression of autophagy gene Beclin 1 was examined by reverse transcriptase polymerase chain reaction(RT-PCR) technique. RESULTS:Docetaxel inhibited the proliferation of cervical cancer HeLa cells in a dose-dependent and time-dependent manner(P

Objective To evaluate the efficacy and safety of percutaneous transluminal angioplasty and stenting of intravascular stenting (PTAS) and internal medicine in the treatment of secondary severe stroke in young patients with severe symptomatic middle cerebral artery stenosis.Methods The clinical data of 77 cases with severe symptomatic middle cerebral artery stenosis(ste nosis rate≥70％)confirmed by digital subtraction angiography(DSA) were collected retrospectively in our hospital from January 2011 to June 2015.The patients were divided into PTAS group and medical treatment group,and the data were collected including the modified Rankin Score (mRS score) at admission,the US National Institutes of Health Stroke volume neurological impairments score (NIHSS score)at admission,as well as mRS score,the recurrence of ischemic stroke,death and intracerebral hemorrhage within 1 year.Results The primary end-point rates within 30 days after enrollment in PTAS group and medical treatment group were 0％ and 5.77％ respectively,and the stroke recurrence rates within 1 year were 4.35％ and 13.46％ respectively,The differ ence was not statistically significant(P＞0.05).Meanwhile,there was no death and intracerebral hemorrhage in both two groups.The rates of mRS≤1 were 91.30 ％ and 69.23 ％ respectively in PTAS group and medical treatment group,and the difference was statistically significant (P＜0.05).The rates of mRS≤2 were 95.65 ％ and 84.62 ％ respectively in PTAS group and medical treat ment group,and the difference was not statistically significant(P＞0.05).Conclusion PTAS is safe for the severe symptomatic middle cerebral artery stenosis,and is more efficient in dectasing the risk of recurrent stroke in young population compared with medical treatment.