Background and purpose:Kiss-1 and nm23 have been identifi ed as tumor metastasis suppressor genes,and they have been associated with the metastatic potential of breast cancer.The purpose of this study was to evaluate the relationship of Kiss-1 and nm23 expression with lymph node metastasis in breast cancer.Methods:The expression of Kiss-1 and nm23 protein was detected by immunohistochemistry in 70 patients with breast cancer.Results:The positive rate of Kiss-1 and nm23 were 62.86% and 68.57%,38.46% and 50.00% in breast cancer patients with lymph node metastasis,markedly lower than the 77.27%,and 79.55% in patients without lymph node metastasis(P

Objective To investigate the epiderniology of fungemia and provide evidence for clinical therapy.Methods A retro- spective survey was done with the 31 cases of fungemia in our hospital from August 2004 to November 2005.Results More than 80% of the patients suffered from two and more underlying diseases.Over a half of infections developed following placement of catheters.And 83.9% of the patients had a history of antimicrobial agents use before blood culture.The pathogens of 24 (77.4%) cases were associated with Candida spp.Only 3 strains were C.albicans.The mortality rate of candidemia was 45.8%.Different Candida species had different resistance rates to antifungal agents.Conclusions Fungemia patients often have serious underlying diseases.Most fungemia cases were candidemia caused by non-C.albicans.Some fungal pathogens are re- sistant to fluconazole and itraconazole.

A hydroponic experiment carried out to study the effect of five Cd levels on growth and photosynthesis of two tomato cultivars showed that the addition of 0.1 micromol/L Cd induced a slight increase in plant height of Hezuo 903 and the SPAD (the Soil-Plant Analyses Development) value of the 2 cultivars. However, at higher Cd levels, i.e., 1 and 10 micromol/L, root length and volume, plant height, and SPAD value were all significantly reduced. On an average of the 2 cultivars, exposure to 1 and 10 micromol/L Cd for 33 d reduced plant height by 18.9% and 46.4% and SPAD value by 11.2% and 31.6%, compared with control, respectively. Similarly, root length was reduced by 41.1% and 25.8% and root volume by 45.2% and 63.7%, respectively. The addition of Cd in the growth medium also had significant deleterious effect on net photosynthetic rate (Pn) and intracellular CO(2) concentration (Ci), with Pn being reduced by 27.2% and 62.1% at 1 micromol/L and 10 micromol/L Cd treatments compared to the control, respectively, while Ci increased correspondingly by 28.4% and 39.3%.
Cadmium ; toxicity ; Lycopersicon esculentum ; drug effects ; growth & development ; metabolism ; Photosynthesis ; drug effects ; Plant Leaves ; drug effects ; growth & development ; metabolism ; Plant Roots ; drug effects ; growth & development ; Seedlings ; drug effects ; growth & development ; metabolism

Objective Maxillary canine palatal impaction is a common deformity in stomatology and its etiology remains dis-putable.The aim of this study is to investigate the crown-root morphology of the adjacent lateral incisors in patients with palatally impacted maxillary canines using cone-beam computed tomography ( CBCT) in order to explore its etiology. Methods Using CBCT scanning, we examined 94 maxillary canines in 84 patients, including 27 palatally impacted maxillary canines in 24 patients ( the experi- mental group) , 37 buccally impacted maxillary canines in 30 age-and sex-matched subjects ( control group A) , and 30 normal maxil-lary canines in 30 age-and sex-matched subjects ( control group B) .We selected some necessary reference planes, measured the ori-entation and position of the chosen canines, and studied the morphology of the adjacent lateral incisors by CS 3D imaging, followed by statistical analysis of 17 of the linear variables obtained. Results Compared with control group A, the experimental group showed significantly shorter tooth length ([22.930 ±1.849]mm vs [21.240 ±1.651]mm, P<0.05), crown length ([9.270 ±0.559]mm vs [8.150 ±0.889] mm, P<0.05), and mesiodistal width at 4 mm to the cementoenamel junction (CEJ) ([7.260 ±0.579]mm vs [5.900 ±0.581] mm, P<0.05) as well as shorter buccolingual width at the CEJ level, mesiodistal width at 4 mm to the CEJ, buc-colingual width at 4 mm apical to the CEJ, distance from the canine cusp to the coronal and occlusal planes, and coronal angulation of the canine (all P<0.05).There was a negative correlation between maxillary canine palatal impaction and age (P<0.05). Conclusion One of the main causes of maxillary canine palatal impaction is that the adjacent lateral incisor is too small to provide suf-ficient guidance to the canine.The severity of maxillary canine palatal impaction increases with the age of the patient.

Most chemical medicines have polymorphs. The difference of medicine polymorphs in physicochemical properties directly affects the stability, efficacy, and safety of solid medicine products. Polymorphs is incomparably important to pharmaceutical chemistry, manufacturing, and control. Meantime polymorphs is a key factor for the quality of high-end drug and formulations. Polymorph prediction technology can effectively guide screening of trial experiments, and reduce the risk of missing stable crystal form in the traditional experiment. Polymorph prediction technology was firstly based on theoretical calculations such as quantum mechanics and computational chemistry, and then was developed by the key technology of machine learning using the artificial intelligence. Nowadays, the popular trend is to combine the advantages of theoretical calculation and machine learning to jointly predict crystal structure. Recently, predicting medicine polymorphs has still been a challenging problem. It is expected to learn from and integrate existing technologies to predict medicine polymorphs more accurately and efficiently.

Objective To assess the cognitive function of drug intractable frontal lobe or temporal lobe epilepsy patients,and summarize the characteristics and influencing factors of cognitive dysfunction in these patients.Methods Forty-two patients with drug intractable frontal lobe or temporal lobe epilepsy,admitted to our hospital from April 2014 to December 2014,were chosen in our study;in them,17 were frontal lobe epilepsy and 25 were temporal lobe epilepsy.All patients underwent cognitive function assessments,including Wechsler intelligence scales (WAIS-RC) and Wechsler memory scale (WMS),one week before surgery.Results (1) As compared with the norm mean (100),the scores of full scale intelligence quotient (FIQ,87.23 ±10.12) and verbal intelligence quotient (VIQ,86.26±8.79) in patients with epilepsy were decreased,with statistically significant difference (P＜0.05);in the subtests of VIQ,the scores of arithmetic and vocabulary in the frontal lobe epilepsy group were significantly lower than those in the temporal lobe epilepsy group (P＜0.05);multiple factors analysis found the FIQ scores of the group of younger than 14 years old in age of epilepsy onset were significantly lower than those in the group of older than 14 years old (P＜0.05);the FIQ scores ofmultidrug therapy group were significantly lower than those of mono therapy group (P＜0.05).(2) As compared with the norm (100 and 60),the scores of memory quotient (MQ,82.42±8.31) and short-term memory (50.05± 2.69) of epilepsy group were statistically lower (P＜0.05);the scores of MQ and short-term memory of temporal epilepsy group were significantly lower than those of frontal epilepsy group (P＜0.05).Conclusion There are many influence factors for cognitive function of epilepsy patients and the damages of cognitive function in frontal lobe or temporal lobe epilepsy patients are different,which might be related to the distributions of brain functional regions and damage of neural network connection.

The effect of water temperature, stocking density and feeding cycle on the growth of Poecilobdella manillensis juvenile was conducted P. manillensis was conducted respectively under different conditions: water temperatures(18, 22, 26, 30,34, 38 degrees C and CT), stocking density (75, 125, 200, 275, 350 individual/L) and feeding cycle(2, 4, 6, 8, 12, 16 d). After 30 days, survival rate, weight gain rate, specific growth rate were measured. There was a significant correlation between water temperature and specific growth rate (γ = -0.066x2 + 3.543 1x -38.09, R2 = 0.837 9). Based on the regression equation, the specific growth rate of P. manillensis achieved the maximum (9.461 4) at 26.84 degrees C. And the most optimal water temperature was 26-30 degrees C. Meanwhile, the survival rates of P. manillensis was 0 at 38 degrees C in 3 d. There was significant negative correlation between density and specific growth rate (γ = -0.005 7x + 9.197 3, R2 = 0.998 3) and between feeding cycle and specific growth rate (γ = -0.468 2x + 10.574, R2 = 0.998 8).
Animals ; Annelida ; growth & development ; physiology ; Body Size ; Feeding Behavior ; Temperature ; Water ; chemistry

The biological activity of ADCC by anti-CD20 monoclonal antibody was determined by BioGlo™ Luciferase Assay System using Jurkat/NFAT-luc+FcγRIIIa cell line as effector cell and WIL2-S cell line as target cell. The developed method was verified for specificity, precision and accuracy. Anti-CD20 monoclonal antibody showed a dose-response mode by the developed method, and the determination result complied with the following four-parameter equation: y = (A-D)/[1 + (X/C)(B)] + D. The optimized parameters of the method were determined including the antibodies diluted concentration (18,000 ng·mL(-1)), dilution rate (1:5), the ratio of effector cell and target cell (6:1), and induction time (6 h). The values of eight independent tests have passed a statistical test for curve regression analysis, linear or parallelism, which showed the method possessed good specificity. Four different dilute groups of recovery rates sample were determined for 3 times, and the result showed mean relative potencies of (44.39±3.93)%, (72.74±2.78)%, (128.28±7.01)% and (168.19±2.70)% respectively, with a variation coefficient of less than 10%, and the recoveries of (88.78±7.85)%, (96.99±3.70)%, (102.63±5.61)% and (112.12±1.80)% respectively. A novel reporter gene method for determination of biological activity of ADCC by anti-CD20 monoclonal antibody was successfully developed, which showed strong specificity, good reproducibility and high accuracy, and might be used routinely.
Antibodies, Monoclonal, Murine-Derived ; pharmacology ; Antibody-Dependent Cell Cytotoxicity ; Antigens, CD20 ; immunology ; Genes, Reporter ; Humans ; Reproducibility of Results ; Rituximab

0.05).Conclusions The higher level of SEB-specific IgM and IgE in AD and eczema indi cates the colonization of Staphylococcus aureus,which participates in the exace rbation of allergic inflammation,is involved in the pathogenesis of AD and ecz ema.

Adult ; Cardiovascular Diseases ; physiopathology ; Cardiovascular Physiological Phenomena ; Case-Control Studies ; Female ; Heart ; physiopathology ; Humans ; Pregnancy ; Sports ; Stress, Psychological ; physiopathology