Animals ; Antioxidants ; pharmacology ; Cerebellum ; cytology ; Dietary Supplements ; Drugs, Chinese Herbal ; chemistry ; Fruit ; chemistry ; Male ; Muscle Fatigue ; drug effects ; Neurons ; drug effects ; pathology ; Oxidative Stress ; drug effects ; Physical Exertion ; Rats ; Rats, Wistar ; Vegetables ; chemistry

Specific activation of toll-Like Receptor 9 (TLR9) by ligand CpG DNA/ODN can initiate downstream signaling.The activation of TLR9 signaling is regulated by Granulin,high-mobility group box-1 (HMGB1) and soluble TLR9.Interestingly,TLR9 is no longer only presented in the immune cells,but also expressed in many kinds of tumor cells.TLR9 is involved inmaintaining and promoting the malignant biological behaviors of tumor cells.Elucidation of the function of TLR9 inherent signaling pathways in the tumor cells will provide a novel idea for oncotherapy.

Breast Neoplasms ; therapy ; China ; Female ; Humans

Antineoplastic Agents, Hormonal ; therapeutic use ; Breast Neoplasms ; chemistry ; therapy ; Carcinoma, Intraductal, Noninfiltrating ; chemistry ; therapy ; Female ; Humans ; Immunohistochemistry ; Medical Oncology ; standards ; Practice Guidelines as Topic ; Receptors, Estrogen ; analysis ; Receptors, Progesterone ; analysis ; Societies, Medical ; Tamoxifen ; therapeutic use ; United States

In eukaryotes,the cell(s) material exchange has long existed in its proliferation and apoptosis,while the exchange of nucleus and cytoplasm only through the shuttle movement of the nuclear pore complex (NPC). NPC in the nuclear membrane,achieves the protein,RNA and other substances's transport task through the shuttle movement between nucleus and cytoplasm of eukaryotes.Specific nuclear pore proteins regulate specific transport path,and because of this,the transport and regulation through the NPC is highly selective and coordination,so the NPC play an important role in gene expression,cell signaling networks and maintain the environment.Some of them will change the structure properties of membrane,affecting mitosis,and some can be combined with the kinase leading to abnormal biochemical reactions,or combined with other biological factors in angiogenesis,cell migration or apoptosis of cells leading to abnormal changes,and leading to tumorigenesis.

Objective To understand the current status of advanced schistosomiasis patients, so as to provide the reference for improving the medical care of them in Shanggao County. Methods The data of advanced patients in Shanggao County were collected from the Information Management System for the Advanced Schistosomiasis Chemotherapy and Assistance in Jiangxi Province and a field survey including the epidemiological investigation physical examinations and B ultrasound examinations was performed in 2014. All the data were analyzed statistically and compared with those in 2010. Results Among the 128 ad?vanced schistosomiasis patients the male female ratio was 2.46 1 the average age was 68.02±6.19 years and the patients old?er than 65 years contributed to 64.84%for the all. The proportions of clinically types of ascites splenomegaly multiple granulo?ma in the colon dwarf general and haemorrhage were 57.81% 12.50% 2.34% 0 26.57% and 0.78% respectively. The ultrasound examinations revealed the average diameter of the main trunk of portal vein was 12.70 ± 1.91mm in 2014 which was significantly broader than that of 12.05 ± 0.34mm in 2010 P < 0.01 . Conclusions The advanced schistosomiasis patents in Shanggao County decreases with years and shows an aging trend. Some advanced schistosomiasis patients are worse therefore the chemotherapy and salvation work still should be strengthened.

Breast Neoplasms ; diagnosis ; genetics ; metabolism ; Carcinoma, Intraductal, Noninfiltrating ; genetics ; metabolism ; Chromosomes, Human, Pair 17 ; genetics ; Female ; Genetic Heterogeneity ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Neoplasm Metastasis ; Neoplasm Recurrence, Local ; Polyploidy ; Receptor, ErbB-2 ; genetics ; metabolism

Objective To investigate the characteristics of voice changes by multi-dimensional acoustic analysis in patients who underwent thyroid gland lobectomy without laryngeal nerve injury.Methods To observe vocal cord movement and evaluate voice quality,32 patients with unilateral thyroid adenoma received the examinations of fiberlaryngscopy and Multi-Dimensional Voice Program before and after operation,respectively.Forty subjects with normal voice were used as cantrol group.Results Fiberlaryngscopy examinations were normal in all patients before and after surgery.In thyroid group,the preoperative acoustic parameters in male patients showed no significant difference(P＞0.05) compared with the control group.There was no significant difference between preoperative and postoperative acoustic parameters(P＞0.05).While the postoperative acoustic parameters of female patients was significantly lower than those of preoperative and in control group(P＜0.05).There was significant improvement 3 months after the operation compared with that of preoperative group(P＜0.05).Fhi of 3 months after operation was still lower than those of preoperation and in control group in male and female patients that continues to be significant after 3 months.Conclusion Voice changes may occur after unilateral thyroid gland lobectomy without any laryngeal nerve injury,and there is little voice change in male patients,while in female patients,voice improvement is observed after operation.Fhi still remaines lower level for a certain of time.

BACKGROUND: Insulin-like growth factor-1 (IGF-1) is a peptide hormone, it has been proved a promotion role on the proliferation of precursor cells. OBJECTIVE: To explore the intravenous injection of IGF-1 on the proliferation, migration and differentiation of neural stem cells in rats after cerebral ischemia. METHODS: Eight adult male SD rats were randomly divided into control group and experimental group, with 40 rats in each group. The rats in two groups were used to prepare models of focal cerebral ischemia using modified suture method, the rats in the experimental group were treated with tail vein injection of IGF-1, according to 100 μg/kg computation, the injection was given for 6 continuous days; in the control group, rats were given equal volume of saline. The rats were decapitated at 7, 14, 21, 28 days following intervention, respectively, and rats in each group were given intraperitoneal injection of the BrdU at 1 day before death. Immunohistochemistry and double staining were applied to detect the expressions of BrdU-positive cells, PSA-NCAM-positive cells, BrdU + PSA-NCAM double-positive cells, and BrdU + MAP2 double-positive cells. RESULTS AND CONCLUSION: The number of BrdU-positive cells and PSA-NCAM positive cells reached the peak at 7 days after ischemia; BrdU + PSA-NCAM double-labeled-positive cells could be detected in ischemic bilateral subependymal zone and dentate gyrus, the number was the most at 7 days, then followed by a gradual decrease; the BrdU + MAP2 double-positive cells began to increase from 14 days, and then gradually increased along with the decrease of BrdU + PSA-NCAM double-positive expression, showing a reverse trend. Intravenous injection of IGF-1 can induce the proliferation, differentiation and migration of neural stem cells in rats following ischemic brain injury.

Objective There have been few research on the relationship between expression of HDAC4 and chemotherapy resistance in human lung adenocarcinoma.The present study aims to investigate the expression and clinical significance of HDAC4 in human lung adenocarcinoma tissues.Methods We selected 72 tissues in lung adenocarcinoma patients with docetaxel-resistant from January 2006 to December 2007 in Department of Oncology and Thoracic Surgery, Nanjing General Hospital of Nanjing Military Region, then evaluated the recent efficacy according to the RECIST criteria and divided the tissues into sensitive(n=32, included complete remission and partial remission) and insensitive(n=40, included stability and progress) groups.The expression of HDAC4 in tissues≥the HDAC4 optimal relative expression cut-off value(78.7) was high level HDAC4 group(n=35), otherwise it was low level HDAC4 group(n=37).QRT-PCR analysis was performed to detect the HDAC4 expression levels in sensitive group and insensitive group.Analyzed the progression free survival in high level HDAC4 group and low level HDAC4 group.Results The expression of HDAC4 was significantly higher in the insensitive group compared with the sensitive group [(1.42±0.30) vs (0.60±0.15), P<0.01].The median progression free survival was significantly shortened in the high level HDAC4 group compared with the low level HDAC4 group (10.2 months vs 5.8 months, P<0.05).ConclusionThe expression of HDAC4 increased in docetaxel-resistant lung adenocarcinoma patients, and it is expected to be a predictive indicator of the resistance of docetaxel.