Animals ; Antioxidants ; pharmacology ; Cerebellum ; cytology ; Dietary Supplements ; Drugs, Chinese Herbal ; chemistry ; Fruit ; chemistry ; Male ; Muscle Fatigue ; drug effects ; Neurons ; drug effects ; pathology ; Oxidative Stress ; drug effects ; Physical Exertion ; Rats ; Rats, Wistar ; Vegetables ; chemistry

Specific activation of toll-Like Receptor 9 (TLR9) by ligand CpG DNA/ODN can initiate downstream signaling.The activation of TLR9 signaling is regulated by Granulin,high-mobility group box-1 (HMGB1) and soluble TLR9.Interestingly,TLR9 is no longer only presented in the immune cells,but also expressed in many kinds of tumor cells.TLR9 is involved inmaintaining and promoting the malignant biological behaviors of tumor cells.Elucidation of the function of TLR9 inherent signaling pathways in the tumor cells will provide a novel idea for oncotherapy.

Breast Neoplasms ; therapy ; China ; Female ; Humans

Antineoplastic Agents, Hormonal ; therapeutic use ; Breast Neoplasms ; chemistry ; therapy ; Carcinoma, Intraductal, Noninfiltrating ; chemistry ; therapy ; Female ; Humans ; Immunohistochemistry ; Medical Oncology ; standards ; Practice Guidelines as Topic ; Receptors, Estrogen ; analysis ; Receptors, Progesterone ; analysis ; Societies, Medical ; Tamoxifen ; therapeutic use ; United States

Breast Neoplasms ; diagnosis ; genetics ; metabolism ; Carcinoma, Intraductal, Noninfiltrating ; genetics ; metabolism ; Chromosomes, Human, Pair 17 ; genetics ; Female ; Genetic Heterogeneity ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Neoplasm Metastasis ; Neoplasm Recurrence, Local ; Polyploidy ; Receptor, ErbB-2 ; genetics ; metabolism

In eukaryotes,the cell(s) material exchange has long existed in its proliferation and apoptosis,while the exchange of nucleus and cytoplasm only through the shuttle movement of the nuclear pore complex (NPC). NPC in the nuclear membrane,achieves the protein,RNA and other substances's transport task through the shuttle movement between nucleus and cytoplasm of eukaryotes.Specific nuclear pore proteins regulate specific transport path,and because of this,the transport and regulation through the NPC is highly selective and coordination,so the NPC play an important role in gene expression,cell signaling networks and maintain the environment.Some of them will change the structure properties of membrane,affecting mitosis,and some can be combined with the kinase leading to abnormal biochemical reactions,or combined with other biological factors in angiogenesis,cell migration or apoptosis of cells leading to abnormal changes,and leading to tumorigenesis.

Objective To understand the current status of advanced schistosomiasis patients, so as to provide the reference for improving the medical care of them in Shanggao County. Methods The data of advanced patients in Shanggao County were collected from the Information Management System for the Advanced Schistosomiasis Chemotherapy and Assistance in Jiangxi Province and a field survey including the epidemiological investigation physical examinations and B ultrasound examinations was performed in 2014. All the data were analyzed statistically and compared with those in 2010. Results Among the 128 ad?vanced schistosomiasis patients the male female ratio was 2.46 1 the average age was 68.02±6.19 years and the patients old?er than 65 years contributed to 64.84%for the all. The proportions of clinically types of ascites splenomegaly multiple granulo?ma in the colon dwarf general and haemorrhage were 57.81% 12.50% 2.34% 0 26.57% and 0.78% respectively. The ultrasound examinations revealed the average diameter of the main trunk of portal vein was 12.70 ± 1.91mm in 2014 which was significantly broader than that of 12.05 ± 0.34mm in 2010 P < 0.01 . Conclusions The advanced schistosomiasis patents in Shanggao County decreases with years and shows an aging trend. Some advanced schistosomiasis patients are worse therefore the chemotherapy and salvation work still should be strengthened.

Objective To explore the surgical method and effect of arthroscopy assisted minimally invasive therapy in the treatment of tibial plateau fractures .Methods Under arthroscopy surveillance ,32 patients with tibial plateau fracture ( Schatzker Ⅰ-Ⅳ) were taken reduction and fixation .Results All cases were followed up for 4-39 months .All fractures were unitied 3-6 months after operation ,and no joint infection compartment syndrome or ankylo-sis occurred.The results were excellent in 25 patients,good in 7 cases according to the HSS evaluating system 9 months after operation .Conclusion For tibial plateau fractures under arthroscopy assisted reduction and minimally invasive plate osteosynthesis has little injury ,exact reset fixation ,early recovery of the knee joint function ,the curative effect is satisfied .

Objective To recognize the possibility of Y fragment deletion of Amelogenin gene intuitively and simply according to the genotyping graphs.Methods By calculating the ratio of total peak height of genotyping graphs, the statistics of equilibrium distribution between Amelogenin and D3S1358loci,Amelo-genin X-gene and Amelogenin Y-gene, and different alleles of D3S1358loci from 1968 individuals was analyzed after amplified by PowerPlex? 21 detection kit.Results Sum of peak height of Amelogenin X allele was not less than 60% that of D3S1358loci alleles in 90.8% female samples, and sum of peak height of Amelogenin X allele was not higher than 70% that of D3S1358loci alleles in 94.9% male samples.Conclusion The result of genotyping after amplified by PowerPlex? 21 detection kit shows that the possibility of Y fragment deletion should be considered when only Amelogenin X-gene of Amelo-geninis detected and the peak height of AmelogeninX-gene is not higher than 70% of the total peak height of D3S1358loci.

Objectiye To study the mechanism by which transcranial magnetic stimulation (rTMS) affects cognitive dysfunction in vascular dementia (VD). Methods Thirty-six male Wistar rats were randomly divided into a control group, a VD group, a low frequency rTMS group and a high frequency rTMS group. Two-vessel occlusion was employed to induce VD models. Low frequency rTMS group rats were given 0.5 Hz rTMS for six weeks. High frequency rTMS group rats were given 5 Hz rTMS for six weeks. Morris' water maze test was used to measure their spatial learning ability and memory. The ultrastructures of the synapses in the four groups were detected with transmission electron microscopy. The expression of synaptophysin (SYN), brain derived neurotrophic factor (BDNF) and Nmethyl-D-aspartate receptor 1 ( NMDAR1 ) mRNA and protein in the hippocampus were determined by real-time PCR and Western blotting. Results The behavior and morphology of the rats treated with rTMS improved. The average expression of SYN, BDNF and NMDAR1 mRNA and protein in the low frequency rTMS group and the high frequency rTMS group were significantly higher than in the VD group. Conclusion rTMS can provide a rehabilitative effect for VD. The mechanism might be associated with enhancing the expression of SYN, BDNF and NMDAR1 in the hippocampus.