AIM: To study the pharmacodynamics of Guanhuang Ganmao Capsule. METHODS: Antiviral effect of Guanhuang Ganmao Capsule was investigated by infecting chicken embryo with N_1H_1; its antipyretic action was evaluated with rabbit pyrogentic test induced by bacterial endotoxin; anti-inflammation by rats swelling test with injection of 1% agar and mice abdominal capillary permeability test with dimethylbanzene; the antitussive activities were investigated through testing cough times induced by ammonium hydroxide and sulfur dioxide in mice; the expectorant activities of it were studied by testing the amount of phlegm secreted in mice; The antibacterial effects in vivo were evaluated in mice with induced systematic infection. RESULTS: Guanhuang Ganmao Capsules had obvious antiviral action, antipyretic action, anti-inflammation and antitussive action. It took expectorant effect. The capsules markedly reduced death rate of staphylococcus aureus infection in mice. CONCLUSION: Guanhuang Ganmao Capsules possess antiviral; antipyretic; antitussive and expectorant effects as well as anti-inflammation

OBJECTIVE:To study the heal-promoting action of liuwei aloe ointment on infectious burning and blanching wound.METHODS:The model rats with third-degree burn infected with staphylococcus aureus and the model mice with superficial second-degree burn infected with bacillus pyocyaneus were randomly divided into excipient group,Jingwanhong positive control group,and liuwei aloe low,medium,and high dose group,with10rats and mice in each group.The wound area and the wound healing time were recorded,and the wound healing rate was calculated.The healing wound was evaluated by histopathological scoring.RESULT:Liuwei aloe ointment at different doses could all significantly promote the heal ofburn wound(P

Objective To investigate the expression of brain-type glycogen phosphorylase(GPBB)as a new biomarker in colorec-tal carcinomas,to understand its expression timing in colorectal neoplasia and its relation with the clinical pathological parameters, and to compare it with the P53 gene.Methods 40 specimens of colorectal carcinoma and 18 specimens of colorecpal neoplasia were selected.The expression of GPBB and P53 in colorectal tumor was investigated by self-made specific anti-human GPBB antibody and P53 antibody.Results The positive expression rate of GPBB in colorectal carcinoma was 80%,which was significantly higher than that in colorectal carcinoma,moreover its expression in moderate and severe atypical hyperplasia adenoma and papillary adenoma was earlier than P53 gene expression.Conclusion GPBB is a enzyme expressed in carcinoma and precancerous lesion,therefore it may be taken as early biomarker enzyme for predicting the occurrence of colorectal carcinoma.

Objective To analyze the hepatitis B virus infected patients'B-ultrasound hepatobiliary imaging and provide a refer-ence for infection prevention and diagnosis of hepatobiliary disease in the region of Shijiazhuang.Methods In three hospitals of Shi-jiazhuang,500 cases of hepatitis B virus infected patients were enrolled in the study.All patients had intact liver function test data and hepatobiliary B-ultrasound information,and their liver function test results and hepatobiliary B-ultrasound imaging test results were analyzed.Results Among 500 cases of patients with hepatitis B virus infection,22.4% patients were with alanine aminotrans-ferase (ALT)increasing,diagnosed by using B-mold ultrasonography 5.4% patients with a liver cyst,2.2% with liver abscess,8. 4% with fatty liver,1.4% with primary liver cancer percent,1.2% with secondary liver cancer,1.0% with cirrhosis.Conclusion The liver function test of patients suffering from fatty liver,cirrhosis,liver abscess shows higher proportion of ALT increasing, which suggests that in patients with hepatitis B virus infection whose liver function tests display ALT elevations should underwent B ultrasonic examination and doctors should focuse on if the patient with cirrhosis,fatty liver or liver abscess.

Objective To detect the expression and activation level of nuclear factor ?B (NF-?B) in synovium of rheumatoid arthritis (RA).Method Forty-six specimens including 17 RA, 24 osteoarthritis (OA) and 5 normal synovial tissues were subjected to RT-PCR to determine the mRNA level of NF-?B p65,p50,inhibitor of NF-?B (I?B),interleukin-1? (IL-1?) and matrix metalloproteinase 9 (MMP-9).Thirty-nine sections including 14 RA,21 OA and 4 normal synovium were subjected to immunohistochemistry to determine the expression level of NF-?B p65.Synoviocytes from 14 synovial specimens including 8 RA and 6 OA were cultured and the nuclear protein was extracted and reserved for western blot.Results The mRNA level of p65,IL-1(,MMP-9 were higher in RA group than that of control (P

Objectives To construct two retroviral vectors, one containing human interleukin-1 recep-tor antagonist (hIL-1Ra) gene and the other containing human interleukin-10 (hIL-10) gene and to transfect rabbit synoviocytes in vitro and detect the expression level of target genes. Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR. The target genes were cloned into retroviral vector pLXSN, which was then transducted into GP2-293 cells to produce recombinant retrovirus. Rabbit synoviocytes were transfected and the expression of target genes was detected by RT-PCR, immunohistochemistry and western-blot. Results The retroviral vector containing hIL-1Ra gene or hIL-10 gene was constructed successfully. The hIL-1Ra gene and hIL-10 gene were transduced respectively into rabbit synoviocytes in vitro. The mRNA level of both genes reached peak in 5 days. In positive cell clones, the protein level of hIL-1Ra reached peak within 30 days and maintained at least 60 days; the protein level of hIL-10 maintained at least 40 days. Conclusion The hIL-1Ra gene and hIL-10 gene can be transduced successfully into rabbit synoviocytes by recombinant retrovirus.

Objective To study the effects of Nerve Regeneration Factor on the neurite growth and Neurofilament-H expression of rat dorsal root ganglions in vitro. Methods DRG was cultured and treated with NRF,observation made by fluorescent immunocytochemistry and the mRNA and protein level of NF-H of DRG neurons was detected by real-time fluorescence quantitative RT-PCR and Western blot. Results NRF can promote the neurite growth of DRGs in vitro.The mRNA and protein levels of NF-H of DRG neurons can be up-regulated by NRF in a dose-dependent manner,with the best concentration of 2.0?(mg/L.) Conclusion\ NRF can promote the neurite growth of DRGs in vitro.NRF exerts a neurotrophic action on developmental sensory neurons.

Objective To seek for the best clinical nursing pathway for early limbs activity of patients with high-risk hemiplegia in the department of cerebral surgery and to reduce limbs paralysis and improve their life quality.Methods Patients(112 cases)with high-risk bemiplegia in the department of cerebral surgery from January to December in 2007 were set as the experimental group.They took part in limbs early activity on the first day on admission with application of clinical nursing pathway.Patients(89 cases)of the same kind who took part in limbs activity from January to December in 2006 were set as the control group.The two groups were compared with Brunnstrom scale beth on admission and discharge.Results The Brunnstrom grade of upper limbs,hands and lower limbs of the experimental group was evidently higher than that of the control group(P＜0.05).Especially the isolated movement and coordination ability of the hands significantly increased(P＜0.01). Conclusions Early limbs activity with clinical nursing pathway for patients with high-risk hemiplegia in the department of cerebral surgery could reach the aim of recovery of limbs functional activity.

0.05). Conclusion CCM is a proper scaffold,on which KC and FB of tympanum maintain satisfactory proliferation and collagen secretion activity.

Objective To explore causes and solutions of false estimates of elevated serum creatinine in patients with Walden‐strom′s macroglobulinemia throug wet chemical enzymatic method .Methods 5 cases of patients hospitalized in the Bethune Inter‐national Peace Hospital were enrolled as subjects from 2010 to 2012 .The large molecular proteins were removal from serum sam‐ples collected from patients with Waldenstrom′s macroglobulinemia by using centrifugal ultrafiltration tube .The serum creatinine levels were detected through using the wet chemical enzymatic method ,wet chemical picric acid method and dry chemical enzymatic method before and after ultrafiltration ,and data were compared .Results Before ultrafiltration ,the levels of serum creatinine of 2 cases of patients with Waldenstrom′s macroglobulinemia detected by using wet chemical enzymatic method differed with those de‐tected by using wet chemical picric acid method and dry chemical enzymatic method .While there were no obvious differences be‐tween levels of serum creatinine detected by wet chemical picric acid method and dry chemical enzymatic method .While ,after ultra‐filtration ,no obvious differences were founded in levels of serum creatinine detected by the thress methods .Conclusion The large molecular proteins should be eliminated when using the wet chemical enzymatic method in the detection of serum creatinine levels , in order to avoid abnormal increase .And the wet chemical picric acid method and dry chemical enzymatic method could also be uti‐lized to determine the accuracy ,and provide reliable determination results .