Objective To establish a cell model that is close to the HCV replication in vivo and can support long-term HCV replication in vitro.Methods A human liver cell line 7721 was inoculated with serum from a chronic hepatitis C patient for 8 hours.After incubation,the presence of HCV RNA,the expression of HCV antigen and the location of HCV RNA in cells and/or supernatant were detected by RTPCR,immunohistochemistry and in situ hybridization respectively.Results It was found that plus-and minus-strand of HCV RNA could be discontinuously detected in both cells and supernatant as late as 66 days after inoculation even if cells had been subcultured for 6 times.HCV NS3、NS5 antigens could be expressed in cells and HCV RNA was mainly located within cytoplasm.Conclusion The above results suggested that 7721 cell line was not only susceptible to HCV but also could support its long-term replication in vitro.This HCV infection model in vitro was proved to be a useful tool for studying the mechanism of HCV infection and replication,as well as evaluating the antiviral compounds and screening the protective antibodies/vaccines.

AIM: To study whether there was a correlation between central foveal thickness (CFT) assessed with optical coherence tomography (OCT) and visual acuity of patient with high myopia after phacoemulsification and intraocular lens implantation.METHODS: Totally 67 patients with high myopia underwent phacoemulsification and intraocular lens implantation were enrolled in the study.Best corrected visual acuity (BCVA) was recorded and CFT was measured using OCT at 1wk,1 and 3mo after oerations.BCVA and CFT were compared before and after the operation.All patents were divided into two groups by the BCVA at 3mo after operation,BCVA>0.5 in Group A and BCVA≤0.5 in Group B.ANOVA,Spearman correlation analysis and independent t test were used for statistical analysis.RESULTS: There was a statistically significant difference between preoperative BCVA and postoperative BCVA (F=115.04,P<0.01).Preoperative CFT was different compared with that 1wk and 1mo after operation (P=0.04,0.02) and was not different with that 3mo after operation(P=0.52).There was a statistically significant difference in CFT of postoperative 3-month compared with that of postoperative 1-week(P<0.01) or that of postoperative 1-month (P<0.01).BCVA showed significant positive correlation with CFT without foveal lesion on postoperative 3mo (r=0.28,P=0.03).CFT of Group A and Group B was significantly different at 3mo after the operation (t=-2.24,P=0.03).There was no significant difference in age and intraocular lens of two groups.CONCLUSION: Optical coherence tomography allow for objective assessment of retinal construction changes in eyes with high myopia are correlated to visual acuity.

There is remarkable heterogeneity in the history of the bladder cancer,which makes it essential to evaluate individualized prognosis risk of each patient.The Nomograms model is more convenient and accurate than other prognostic models,can provide significantly accurate individualized risk estimations that facilitate management decisions.The Nomograms model has been regarded as the most accurate and discriminating prognosis model to the patient of bladder cancer,and its development has a bright future.While the Nomograms Model has several weakness itself,which would be remedied through more researches and new predictors.Before Nomograms Model can be accepted in practice,we really need better evidence that they improve patient care and outcomes.

Objective To investigate the inhibition effects of different antihistamines on the expressions of chemokines and cytokines released by nasal polyps in vitro.Methods The fresh nasal polyps resected under endoscope from the patients admitted to our hospital were cultured with mizolastine,cetirizine,loratadine,fexofenadine,dexamethasone,or ciclosporin A respectively for 24 h,with the addition of histamine and arachidonic acid.The total RNA of polyps was obtained and purified with tripure isolation reagent.The mRNA expression levels of monocyte chemoattractant protein-1(MCP-1),monocyte chemoattractant protein-3(MCP-3),regulated on activation,normal T cell expressed and secreted(RANTES),eotaxin were analyzed by RT-PCR.The concentrations of interleukin(IL)-4,IL-5 and tumor necrosis factor-?(TNF-?)in cultured supernatant was detected by ELISA.Results The MCP-1 mRNA expression in nasal polyps treated by mizolastine was weaker than those by loratadine and fexofenadine;the MCP-3 mRNA expression by mizolastine was weaker than those by cetirizine and loratadine;the RANTES mRNA expression by mizolastine was weaker than those by loratadine and fexofenadine;the eotaxin mRNA expression by mizolastine was weaker than that by cetirizine(all P

Four synthetic peptides,CP10,CP9,CP33c and CP100,with the sequences corresponding to the capsid protein and nonstructural protein of hepatitis C virus(HCV)respectively,were employed to establish ELISA to detect anti-HCV.Serum HCV RNA was identified with reverse transcriptive polymerase chain reaction(RT - PCR)for comparison.Out of 320 sera collected from patients with viral hepatitis,patients with other diseases and blood donors,49 were positive for antibodies against a single or several peptides.Among the positive rates of antibodies against a single peptide,anti-CP10 rate(9.69%)was the highest and anti-CP33c(3.44%)the lowest.The positive rate for anti-HCV antibodies was significantly higher when 4 peptides were combinedly used than when only one peptide(P

Humans ; Infant ; Male ; Mycoses ; pathology ; Penicillium ; isolation & purification

Objective To investigate the clinical value of snapshot assist (SSA) of the gemstone CT in prospective ECG-gated coronary artery angiography in reducing radiation dose.Methods A total of 59 patients with prospective ECG-gated coronary angiography were enrolled,who were divided into SSA group and routine group.The SSA group was scanned with SSA technique.The difference were compared between SSA group and conventional group in image quality,dose length product (DLP) and effective dose (ED).Results There was no significant difference of image quality scores between the two groups (x2 =2.362,P=0.307).There was also no significant difference in the mean CT value of the aorta root between the two groups (Z=-0.061,P 0.951).While the differences of noise,CNR and SNR were statistically significant (all P＜0.05).The DLP and ED of SSA group were (135.44±37.41)mGy · cm and (1.89±0.52)mSv.And the DLP and ED of routine group were (275.57 ±36.59)mGy · cm and (3.85 ±0.51)mSy.There were significant differences between the two groups (t=14.504,P＜0.01).The ED reduced about 50.85％ in SSA group compared with that in routine group.Conclusion The SSA technology can reduce the effective radiation dose of patients in prospective ECG gated coronary angiography,and has a high clinical value.

Objective To observe the histopathological features of early skin lesions and serum inflammatory mediators changes in the diabetes mellitus(DM) rats.Methods A total of 50 Wistar rats at 8 to 10 weeks of age were divided into normal control group(C group,n=25) and diabetic group(DC group,n=25).The rats of DC group were given a high-glucose and high-fat diet for 2 weeks to induce insulin resistance and then an intraperitoneal injection of 35 mg/kg streptozotocin.All 25 rats were identified to be diabetic models with their fasting blood glucose over ≥7.0 mmol/L.Four rats of C group or DC group were killed to take the skin tissues in the 4th,8th and 12th weekend.In the 16th week,the heart blood samples of the left 13 rats of C group and 10 rats of DC group were collected to measure TNF-?,IL-6 and C-RP levels,and the skin samples were also taken for pathological observation and full-thickness and dermal thickness measurement.Results In the 16th week,the TNF-?,IL-6 and C-RP levels of DC group were significantly higher than the normal group(P

Objective To investigate the effects of rosiglitazone on serum levels of tumor necrosis factor-? (TNF-?),interleukin-6 (IL-6),C-reactive protein (C-RP) and superoxide dismutase (SOD),and early skin changes in the diabetic rats.Methods The rats were divided into normal control group (C group,n=13),diabetic control group (DC group,n=13),diabetes and insulin treatment group (DI group,n=13),diabetes and rosiglitazone treatment group (DR group,n=13).The diabetic rat models were established with high-fat diet for 2 weeks followed by intraperitoneal injection of 35 mg/kg streptozotocin injection,the rats were idenfied as diabetic when their fasting blood glucose (FBG) over 7.0 mmol/L.The living rats were given corresponding treatment,subcutaneous injection of 1 to 2 U/d insulin,intragastric injection of 5 mg/(kg?d) rosiglitazone,or sterile water.Heart blood samples of rats from each group were collected to measure TNF-?,IL-6,C-RP and SOD levels in the 16th weekend.Also the skin was taken for pathological observation and full-thickness as well as dermal thickness measurements.Results The serum levels of IL-6,TNF-? and C-RP in diabetic group were significantly higher than those in normal group (P

Objective To establish a simple animal model and the cytotoxic T cell(CTL) detecting system for the studies of the effects of CTL on HCV infection. Methods The CTL activity in BLAB/c mice immunized by polypeptides in the core region of HCV was detected with lactic dehydrogenase (LDH) by using SP2/O cells as the target cells. Results CTL activity in BLAB/c mice immunized by polypeptides in the core region of HCV could be detected with LDH. The activity could be enhanced by CPA10(5～23 aa) but inhibited by CPA9(39～74 aa). Conclusion CTL activity in BLAB/c mice can be detected stably by LDH.