Integron was a novel bacterial resistance gene horizontal transmission element.In recent years, many researchers made a lot of research on the resistance mechanism of pathogen.In this paper, the detection and the novel discovery of the integron gene cassette were summarized , including Enterobacteria, Acinetobacter baumanii, Pseudomonas aeruginosa and other bacteria , on the basis of the multidrug resistance mechanism mediated by integron.The prospect the research was descixbed and more attention should be paid to the prevention and control of multidrug resistant bacteria.

Animals ; Astrocytes ; metabolism ; Brain Ischemia ; metabolism ; pathology ; Glial Fibrillary Acidic Protein ; metabolism ; Neuroprotective Agents ; pharmacology ; Propofol ; pharmacology ; Random Allocation ; Rats ; Rats, Wistar ; Reperfusion Injury ; metabolism ; prevention & control

Objective To investigate the value of selective water excitation technique for the assessment of articular cartilage.Methods MR sagittal scanning of knee joints was performed in the fifteen healthy volunteers.MR scan sequences were 3D-FFE-SPIR and 3D-FFE-WATS.The signal noise ratio (SNR)of the cartilage,the contrast noise ratio(CNR)between cartilage and adjacent tissue and their efficiency were calculated and analyzed statistically.Tweenty-nine patients who were suspected having cartilage injury were performed MR examination anti the image characteristics and the detecting ability of each sequence on cartilage lesions were analyzed.Results In the healthy volunteers,the cartilage SNR was 3D-FFE-SPIR:197.93?18.58,3D-FFE-WATS:187.32?21.50(P=0.159).CNR(cartilage/bone)was 3D-FFE-SPIR:185.50?18.34,3D-FFE-WATS:169.55?24.57(P=0.054).CNR(cartilage/muscle)was 3D-FFE-SPIR:61.40?19.04,3D-FFE-WATS:47.27?21.05(P=0.064).The cartilage SNR and CNR between cartilage and bone,muscle of 3D-FFE-SPIR weren't significantly higher than that of 3D-FFE- WATS.CNR(cartilage/liquid)was 3D-FFE-SPIR:91.53?14.46,3D-FFE-WATS:149.28?32.30(P= 0.000).CNR(cartilage/marrow)was 3D-FFE-SPIR:159.26?18.83,3D-FFE-WATS:176.87? 22.50(P=0.028).CNR(cartilage/fat)was 3 D-FFE-SPIR:134.56?15.80,3D-FFE-WATS:154.01? 22.42(P =0.010).The CNR between cartilage and liquid,marrow,fat were higher in 3D-FFE-WATS and significantly different than that of 3D-FFE-SPIR.Thirty detected cartilage injuries of patients were 3D-FFE- WATS:39,3D-FFE-SPIR:45 and there was no statistical difference between them(P=0.37). Conclusion 3D-FFE-WATS can show the articular cartilage clearly.It has high scan speed and suppress the fat signal evenly.Its ability for finding cartilage damage is equal to that of 3D-FFE-SPIR.So WATS can be used in the routine clinical cartilage examination.

Objective To discuss the effect of interferon ?-2a on the radiosensitivity and cell cycle for nasopharyngeal carcinoma cell line CNE-I. Methods Interferon ?-2a was given by different concentration. Then the cells were radiated with X-ray (6?MV) and the cell survival rate was calculated. The change of cell cycle dynamics was measured with flow cytometry. Results The cell survival fraction was 0.62, 0.43 and 0.20 respectively after the interferon ?-2a was given by different concentration(0.5?106, 1.0?106 and 1.5?106?IU/L). The radiosensitization ratio was 1.16, 1.57 and 1.93 respectively. Compared with the control group, increasing cell percentage in G_1 and G_2+M phage and decreasing cell percentage in S phage were observed on 24 h after the interferon ?-2a(1?106?IU/L) was given(P

AIM　To Detect the varying of gene expression in cultured dorsal root ganglion cells treated by Nerve Regeneration Factor and explore its molecular mechanism promoting to nerve growth. METHODS　By RT-PCR, the change of gene expression in GAP-43 and NF-L on the cultured DRG cells with Nerve Regeneration Factor were studied during 4 h, 12 h, 24 h. RESULTS　The expression of GAP-43 and NF-L were increased by Nerve Regeneration Factor on DRG′s culture. CONCLUSION　This study indicated Nerve Regeneration Factor may up-regulate the gene expression associated nerve growth on cultured nervous cells.

Animals ; Heart ; growth & development ; Myocardium ; metabolism ; Nerve Growth Factors ; genetics ; metabolism ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley

Objective To investigate the correlation between nocturnal penile tumescence (NPT) test results and metabolic syndrome (MS).Methods In this prospective study,75 cases were enrolled,including 25 healthy volunteers (group A),25 erectile dysfunction (ED) patients with normal NPT results (group B),and 25 ED patients with abnormal NPT results (group C).At the time of recruitment,all the participants had serum total testosterone higher than 9.9 nmol/L,and were free of MS.At baseline,there was no significant differences in waistline,body mass index,fasting plasma glucose (FPG),mean arterial pressure (MAP),triglyceride,high density lipoprotein (HDL),cholesterol,and serum total testosterone among the three groups (P＞0.05).And there was no significant difference in international index of erectile function 5 score between group B and C.A follow-up survey was conducted three years after baseline to measure the above metabolic indicators and to compare the incidence of MS among the three groups.Results In the three-year follow-up survey,2 participants in group A were lost to follow-up,compared to 3 participants in group B and 5 participants in group C.In the three-year follow-up survey,there was no significant difference in all the indicators between group A and B; between group A and C,significant differences were observed in MAP,FPG,HDL,and cholesterol (P＜0.05); between group B and C,significant differences were observed in MAP and HDL (P＜0.05).In the three-year follow-up survey,2 participants in group A developed MS,compared to 3 MS patients in group B and 7 MS patients in group C.There was a significant difference in the incidence of MS among the three groups (P＜0.05).Conclusions To some extent,NPT reflects and predicts the development and progress of MS.Therefore,NPT can be used as an early indicator of MS.

ObjectiveTo study the occurrence and influence factors of drug-induced injuries of vital organs caused by traditional Chinese medicine injection.MethodsChina Hospital Knowledge Database (CHKD) from 1993-2013 and case reports concerning adverse effects and drug-induced diseases caused by traditional Chinese medicine injection from Wanfang Data Knowledge Service Platformwere retrieved. Indicator systemwasestablished and relevant contentsweresummarized and analyzed.Results34 types of traditional Chinese medicine injectionswereincluded and 699 drug-induced diseasesweresummarized, taking up 53.98% of the total adverse effects. Among them, the top three included in the drug-induced diseaseswere acanthopanax injection, safflower injection and Mailuoning injection. The non-conformance between the traditional Chinese medicine injections in the literature and instructions mainly reflects unreasonable solvent selection, large compatibility concentration and usage and dosage beyond those specified in the instructions. ConclusionThe occurrence of drug-induced injuries of vital organs caused by traditional Chinese medicine injectionis relatively high, drug instructions shall be strictly followed in accordance with requirements during clinical application.

Purpose To assess the effect of pelvicfloor electrical stimulation treatment by transperineal sonography, so as to provide guidance for postpartum pelvic floor rehabilitation.Materials and Methods Thirty-one delivery women who received postpartum pelvicfloor rehabilitation were selected as research group and 30 non-pregnant young women as control group in the study. Pelvicfloor electromyography treatment was taken in research group, and transperineal sonography was undergone in quiet state and pressure state (Valsalva operation) before treatment, after the fifth treatment and after the tenth treatment respectively to measure the bladder neck to the public symphysis edge distance (Dr, Ds), bladder neck angle (θr,θs) and bladder rotation angle (θ). A comparison was made between the research group and control group.Results①Dr and Ds in research group were both lower than those in control group (P<0.05), butθr,θs andθ were all higher than those in control group (P<0.05).②Dr and Ds showed a gradual increase (P<0.05) whilstθr presented a gradual decrease before the treatment, after the fifth treatment and after the tenth treatment (P<0.05).Conclusion Natural childbirth may damage the function of pelvicfloor; electrical stimulation of pelvic therapy is effective on postpartum pelvicfloor rehabilitation; and transperineal sonography can objectively assess the effect of this therapy.

Differentiation strategy includes self examination on ability,location and intention,strengthening self rarity according to its own characteristics,and improving core competitive power.This article reviewed several related issues in R&D fields,and suggested to set up new evaluation systems which focus on discipline construction,scientific research output,classified evaluation and representative work.