AIM: To study the pharmacodynamics of Guanhuang Ganmao Capsule. METHODS: Antiviral effect of Guanhuang Ganmao Capsule was investigated by infecting chicken embryo with N_1H_1; its antipyretic action was evaluated with rabbit pyrogentic test induced by bacterial endotoxin; anti-inflammation by rats swelling test with injection of 1% agar and mice abdominal capillary permeability test with dimethylbanzene; the antitussive activities were investigated through testing cough times induced by ammonium hydroxide and sulfur dioxide in mice; the expectorant activities of it were studied by testing the amount of phlegm secreted in mice; The antibacterial effects in vivo were evaluated in mice with induced systematic infection. RESULTS: Guanhuang Ganmao Capsules had obvious antiviral action, antipyretic action, anti-inflammation and antitussive action. It took expectorant effect. The capsules markedly reduced death rate of staphylococcus aureus infection in mice. CONCLUSION: Guanhuang Ganmao Capsules possess antiviral; antipyretic; antitussive and expectorant effects as well as anti-inflammation

OBJECTIVE:To study the heal-promoting action of liuwei aloe ointment on infectious burning and blanching wound.METHODS:The model rats with third-degree burn infected with staphylococcus aureus and the model mice with superficial second-degree burn infected with bacillus pyocyaneus were randomly divided into excipient group,Jingwanhong positive control group,and liuwei aloe low,medium,and high dose group,with10rats and mice in each group.The wound area and the wound healing time were recorded,and the wound healing rate was calculated.The healing wound was evaluated by histopathological scoring.RESULT:Liuwei aloe ointment at different doses could all significantly promote the heal ofburn wound(P

Objective To analyze the hepatitis B virus infected patients'B-ultrasound hepatobiliary imaging and provide a refer-ence for infection prevention and diagnosis of hepatobiliary disease in the region of Shijiazhuang.Methods In three hospitals of Shi-jiazhuang,500 cases of hepatitis B virus infected patients were enrolled in the study.All patients had intact liver function test data and hepatobiliary B-ultrasound information,and their liver function test results and hepatobiliary B-ultrasound imaging test results were analyzed.Results Among 500 cases of patients with hepatitis B virus infection,22.4% patients were with alanine aminotrans-ferase (ALT)increasing,diagnosed by using B-mold ultrasonography 5.4% patients with a liver cyst,2.2% with liver abscess,8. 4% with fatty liver,1.4% with primary liver cancer percent,1.2% with secondary liver cancer,1.0% with cirrhosis.Conclusion The liver function test of patients suffering from fatty liver,cirrhosis,liver abscess shows higher proportion of ALT increasing, which suggests that in patients with hepatitis B virus infection whose liver function tests display ALT elevations should underwent B ultrasonic examination and doctors should focuse on if the patient with cirrhosis,fatty liver or liver abscess.

Objective To investigate the expression of brain-type glycogen phosphorylase(GPBB)as a new biomarker in colorec-tal carcinomas,to understand its expression timing in colorectal neoplasia and its relation with the clinical pathological parameters, and to compare it with the P53 gene.Methods 40 specimens of colorectal carcinoma and 18 specimens of colorecpal neoplasia were selected.The expression of GPBB and P53 in colorectal tumor was investigated by self-made specific anti-human GPBB antibody and P53 antibody.Results The positive expression rate of GPBB in colorectal carcinoma was 80%,which was significantly higher than that in colorectal carcinoma,moreover its expression in moderate and severe atypical hyperplasia adenoma and papillary adenoma was earlier than P53 gene expression.Conclusion GPBB is a enzyme expressed in carcinoma and precancerous lesion,therefore it may be taken as early biomarker enzyme for predicting the occurrence of colorectal carcinoma.

Objective To detect the expression and activation level of nuclear factor ?B (NF-?B) in synovium of rheumatoid arthritis (RA).Method Forty-six specimens including 17 RA, 24 osteoarthritis (OA) and 5 normal synovial tissues were subjected to RT-PCR to determine the mRNA level of NF-?B p65,p50,inhibitor of NF-?B (I?B),interleukin-1? (IL-1?) and matrix metalloproteinase 9 (MMP-9).Thirty-nine sections including 14 RA,21 OA and 4 normal synovium were subjected to immunohistochemistry to determine the expression level of NF-?B p65.Synoviocytes from 14 synovial specimens including 8 RA and 6 OA were cultured and the nuclear protein was extracted and reserved for western blot.Results The mRNA level of p65,IL-1(,MMP-9 were higher in RA group than that of control (P

Objective To seek for the best clinical nursing pathway for early limbs activity of patients with high-risk hemiplegia in the department of cerebral surgery and to reduce limbs paralysis and improve their life quality.Methods Patients(112 cases)with high-risk bemiplegia in the department of cerebral surgery from January to December in 2007 were set as the experimental group.They took part in limbs early activity on the first day on admission with application of clinical nursing pathway.Patients(89 cases)of the same kind who took part in limbs activity from January to December in 2006 were set as the control group.The two groups were compared with Brunnstrom scale beth on admission and discharge.Results The Brunnstrom grade of upper limbs,hands and lower limbs of the experimental group was evidently higher than that of the control group(P＜0.05).Especially the isolated movement and coordination ability of the hands significantly increased(P＜0.01). Conclusions Early limbs activity with clinical nursing pathway for patients with high-risk hemiplegia in the department of cerebral surgery could reach the aim of recovery of limbs functional activity.

OBJECTIVETo compare the difference in the clinical efficacy on post-stroke insomnia between the low-frequency electric stimulation at the acupoints and the conventional western medication.

METHODSOne hundred and twenty patients of post-stroke insomnia were randomized into a low-frequency electric stimulation group, a medication group and a placebo group, 40 cases in each one. In the low-frequency electric stimulation group, the low-frequency electric-pulsing apparatus was used at Dazhui (GV 14) and Shenshu (BL 23), once a day; the treatment of 15 days made one session and 2 sessions were required. In the medication group, estazolam was taken orally, 1 mg each time. In the placebo group, starch capsules were taken orally, 1 capsule each time. All the drugs were taken before sleep every night, continuously for 15 days as one session, and 2 sessions were required. PSQI changes and clinical efficacy were observed before and after treatment in each group.

RESULTSPitlsburgh sleep quality index (PSQI) score was reduced in every group after treatment (all P < 0.01). In the low-frequency electric stimulation group and medication group, the score was reduced much more significantly as compared with the placebo group (both P < 0.01). In the placebo group, 1 case was rejected. The total effective rates were 95.0% (38/40), 92.5% (37/40) and 17.9% (7/39) in the low-frequency electric stimulation group, medication group and placebo group separately. The efficacy in the low-frequency electric stimulation group and medication group was better apparently than that in the placebo group (both P < 0.01).

CONCLUSIONThe low-frequency electric stimulation at the acupoints effectively and safely treats post-stroke insomnia and the efficacy of it is similar to that of estazolam.

Acupuncture Points ; Aged ; Aged, 80 and over ; Electric Stimulation ; Female ; Humans ; Male ; Middle Aged ; Sleep Initiation and Maintenance Disorders ; etiology ; therapy ; Stroke ; complications ; Treatment Outcome

Objective To study efficacy and safety of the levofloxacin in treatment of refractory pulmonary tuberculosis hemoptysis by meta analysis.Methods Pubmed (2000/2014-07),EMbase (2000/2014-07),the Cochrane library (2014,7),the Chinese biomedical literature database (2000/2014-07)were retrieved about the randomized controlled trials of levofloxacin in treatment of refractory pulmonary tuberculosis hemoptysis,and the time was limited from January 2000 to July 2014.After objective evaluation of the retrieved paper quality,Cochrane collaboration with Rev Man 5. 3 software system was applicated to Meta analysis.Results In six reports of the sputum negative rate,levofloxacin group was significantly higher,and the difference was statistically significant (OR=4.21,95%CI:3.08 ~5.75,P<0.0001);adverse reactions in three articles,the difference of adverse reactions incidence had no statistical significance (OR =0.82,95%CI:0.56 ~1.18,P =0.28)between experimental group and control group, respectively.Conclusion The clinical effect of levofloxacin in treatment of refractory pulmonary tuberculosis hemoptysis is significant with a high security, which gives a guidance for clinical treatment and is worth clinical promotion.

Objective To explore causes and solutions of false estimates of elevated serum creatinine in patients with Walden‐strom′s macroglobulinemia throug wet chemical enzymatic method .Methods 5 cases of patients hospitalized in the Bethune Inter‐national Peace Hospital were enrolled as subjects from 2010 to 2012 .The large molecular proteins were removal from serum sam‐ples collected from patients with Waldenstrom′s macroglobulinemia by using centrifugal ultrafiltration tube .The serum creatinine levels were detected through using the wet chemical enzymatic method ,wet chemical picric acid method and dry chemical enzymatic method before and after ultrafiltration ,and data were compared .Results Before ultrafiltration ,the levels of serum creatinine of 2 cases of patients with Waldenstrom′s macroglobulinemia detected by using wet chemical enzymatic method differed with those de‐tected by using wet chemical picric acid method and dry chemical enzymatic method .While there were no obvious differences be‐tween levels of serum creatinine detected by wet chemical picric acid method and dry chemical enzymatic method .While ,after ultra‐filtration ,no obvious differences were founded in levels of serum creatinine detected by the thress methods .Conclusion The large molecular proteins should be eliminated when using the wet chemical enzymatic method in the detection of serum creatinine levels , in order to avoid abnormal increase .And the wet chemical picric acid method and dry chemical enzymatic method could also be uti‐lized to determine the accuracy ,and provide reliable determination results .

Brain metastasis is among the most common complications of non-small celllung cancer (NSCLC) and may obviously influence the survival and quality of life in the NSCLC patients. Recently, the development in traditional treatments, the widely-used micro-molecular targeted drugs, and the combined treatment have improved the overall survival and the quality of life of these patients. In addition, the exploration of new therapeutic targets and cognitive function protective methods for current treatments also deserves at-tention. This review emphasizes the relevant clinical and basic problems and the therapeutic strategies in NSCLC with brain metastases.