OBJECTIVE:To study the heal-promoting action of liuwei aloe ointment on infectious burning and blanching wound.METHODS:The model rats with third-degree burn infected with staphylococcus aureus and the model mice with superficial second-degree burn infected with bacillus pyocyaneus were randomly divided into excipient group,Jingwanhong positive control group,and liuwei aloe low,medium,and high dose group,with10rats and mice in each group.The wound area and the wound healing time were recorded,and the wound healing rate was calculated.The healing wound was evaluated by histopathological scoring.RESULT:Liuwei aloe ointment at different doses could all significantly promote the heal ofburn wound(P

AIM: To study the pharmacodynamics of Guanhuang Ganmao Capsule. METHODS: Antiviral effect of Guanhuang Ganmao Capsule was investigated by infecting chicken embryo with N_1H_1; its antipyretic action was evaluated with rabbit pyrogentic test induced by bacterial endotoxin; anti-inflammation by rats swelling test with injection of 1% agar and mice abdominal capillary permeability test with dimethylbanzene; the antitussive activities were investigated through testing cough times induced by ammonium hydroxide and sulfur dioxide in mice; the expectorant activities of it were studied by testing the amount of phlegm secreted in mice; The antibacterial effects in vivo were evaluated in mice with induced systematic infection. RESULTS: Guanhuang Ganmao Capsules had obvious antiviral action, antipyretic action, anti-inflammation and antitussive action. It took expectorant effect. The capsules markedly reduced death rate of staphylococcus aureus infection in mice. CONCLUSION: Guanhuang Ganmao Capsules possess antiviral; antipyretic; antitussive and expectorant effects as well as anti-inflammation

Objective To investigate the expression of brain-type glycogen phosphorylase(GPBB)as a new biomarker in colorec-tal carcinomas,to understand its expression timing in colorectal neoplasia and its relation with the clinical pathological parameters, and to compare it with the P53 gene.Methods 40 specimens of colorectal carcinoma and 18 specimens of colorecpal neoplasia were selected.The expression of GPBB and P53 in colorectal tumor was investigated by self-made specific anti-human GPBB antibody and P53 antibody.Results The positive expression rate of GPBB in colorectal carcinoma was 80%,which was significantly higher than that in colorectal carcinoma,moreover its expression in moderate and severe atypical hyperplasia adenoma and papillary adenoma was earlier than P53 gene expression.Conclusion GPBB is a enzyme expressed in carcinoma and precancerous lesion,therefore it may be taken as early biomarker enzyme for predicting the occurrence of colorectal carcinoma.

Objective To analyze the hepatitis B virus infected patients'B-ultrasound hepatobiliary imaging and provide a refer-ence for infection prevention and diagnosis of hepatobiliary disease in the region of Shijiazhuang.Methods In three hospitals of Shi-jiazhuang,500 cases of hepatitis B virus infected patients were enrolled in the study.All patients had intact liver function test data and hepatobiliary B-ultrasound information,and their liver function test results and hepatobiliary B-ultrasound imaging test results were analyzed.Results Among 500 cases of patients with hepatitis B virus infection,22.4% patients were with alanine aminotrans-ferase (ALT)increasing,diagnosed by using B-mold ultrasonography 5.4% patients with a liver cyst,2.2% with liver abscess,8. 4% with fatty liver,1.4% with primary liver cancer percent,1.2% with secondary liver cancer,1.0% with cirrhosis.Conclusion The liver function test of patients suffering from fatty liver,cirrhosis,liver abscess shows higher proportion of ALT increasing, which suggests that in patients with hepatitis B virus infection whose liver function tests display ALT elevations should underwent B ultrasonic examination and doctors should focuse on if the patient with cirrhosis,fatty liver or liver abscess.

Objective To investigate the water source pollution by parasitic protozoa in small towns and villages in Shenzhen,China,in order to present the data to establish the national hygienic standard for controlling parasitic protozoa pollution.Methods The samples of water source were collected from 8 village level water plants in Shenzhen.Giardia lamblia and Cryptosporidium were detected,according to the EPA methods,by filtering,rinsing,magnetic isolation and staining.Results Giardia lamblia was found in water sources of 6 water plants and Cryptosporidium was found in one water plant.Conclusion At present,some water sources of village level water plants where the reservoirs were used as the water source in Shenzhen have been polluted by Giardia lamblia and Cryptosporidium,that indicates the potentiality of the water-born-diseases in that areas.

Objective To evaluate the current sanitary situation of the rural household latrine and the effects of decontamination of the feces in some epidemic areas of schistosome in Hubei province.Methods Six villages(4 villages with the sanitary latrines,2 villages with the non-sanitary latrines) were chosen for the investigation and 30 latrines were selected randomly in each village in September of 2007.The structure of the latrine and the situation of the faecal treatment were investigated by questionnaires.At the same time,the feces samples were collected and detected according to the related sanitary standards.Results The rate of the sanitary latrines without maggots,pupas and flies was 95%,while the rate of the non-sanitary latrines with the same situation was 33.33%.After non-hazardous treatment in the three-case-cesspool and the firedamp latrines,the qualified rates of the roundworm eggs were 95.6% and 98.6% respectively;the qualified rates of fecal coliform were both 100%;no schistosome eggs,the alive schistosome eggs and the alive roundworm eggs were detected.The morbidity rate of schistosomiasis of the villages with the sanitary latrines was 43.7%,lower than those with the non-sanitary latrines.Conclusion The faecal treatment of the rural household latrine in epidemic areas of schistosome can improve the sanitation and kill the pathogens in faeces effectively,and also can decrease the morbidity rate of schistosomiasis obviously.

0.05). Conclusion CCM is a proper scaffold,on which KC and FB of tympanum maintain satisfactory proliferation and collagen secretion activity.

OBJECTIVETo compare the difference in the clinical efficacy on post-stroke insomnia between the low-frequency electric stimulation at the acupoints and the conventional western medication.

METHODSOne hundred and twenty patients of post-stroke insomnia were randomized into a low-frequency electric stimulation group, a medication group and a placebo group, 40 cases in each one. In the low-frequency electric stimulation group, the low-frequency electric-pulsing apparatus was used at Dazhui (GV 14) and Shenshu (BL 23), once a day; the treatment of 15 days made one session and 2 sessions were required. In the medication group, estazolam was taken orally, 1 mg each time. In the placebo group, starch capsules were taken orally, 1 capsule each time. All the drugs were taken before sleep every night, continuously for 15 days as one session, and 2 sessions were required. PSQI changes and clinical efficacy were observed before and after treatment in each group.

RESULTSPitlsburgh sleep quality index (PSQI) score was reduced in every group after treatment (all P < 0.01). In the low-frequency electric stimulation group and medication group, the score was reduced much more significantly as compared with the placebo group (both P < 0.01). In the placebo group, 1 case was rejected. The total effective rates were 95.0% (38/40), 92.5% (37/40) and 17.9% (7/39) in the low-frequency electric stimulation group, medication group and placebo group separately. The efficacy in the low-frequency electric stimulation group and medication group was better apparently than that in the placebo group (both P < 0.01).

CONCLUSIONThe low-frequency electric stimulation at the acupoints effectively and safely treats post-stroke insomnia and the efficacy of it is similar to that of estazolam.

Acupuncture Points ; Aged ; Aged, 80 and over ; Electric Stimulation ; Female ; Humans ; Male ; Middle Aged ; Sleep Initiation and Maintenance Disorders ; etiology ; therapy ; Stroke ; complications ; Treatment Outcome

Objective To detect the expression and activation level of nuclear factor ?B (NF-?B) in synovium of rheumatoid arthritis (RA).Method Forty-six specimens including 17 RA, 24 osteoarthritis (OA) and 5 normal synovial tissues were subjected to RT-PCR to determine the mRNA level of NF-?B p65,p50,inhibitor of NF-?B (I?B),interleukin-1? (IL-1?) and matrix metalloproteinase 9 (MMP-9).Thirty-nine sections including 14 RA,21 OA and 4 normal synovium were subjected to immunohistochemistry to determine the expression level of NF-?B p65.Synoviocytes from 14 synovial specimens including 8 RA and 6 OA were cultured and the nuclear protein was extracted and reserved for western blot.Results The mRNA level of p65,IL-1(,MMP-9 were higher in RA group than that of control (P

Objectives To construct two retroviral vectors, one containing human interleukin-1 recep-tor antagonist (hIL-1Ra) gene and the other containing human interleukin-10 (hIL-10) gene and to transfect rabbit synoviocytes in vitro and detect the expression level of target genes. Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR. The target genes were cloned into retroviral vector pLXSN, which was then transducted into GP2-293 cells to produce recombinant retrovirus. Rabbit synoviocytes were transfected and the expression of target genes was detected by RT-PCR, immunohistochemistry and western-blot. Results The retroviral vector containing hIL-1Ra gene or hIL-10 gene was constructed successfully. The hIL-1Ra gene and hIL-10 gene were transduced respectively into rabbit synoviocytes in vitro. The mRNA level of both genes reached peak in 5 days. In positive cell clones, the protein level of hIL-1Ra reached peak within 30 days and maintained at least 60 days; the protein level of hIL-10 maintained at least 40 days. Conclusion The hIL-1Ra gene and hIL-10 gene can be transduced successfully into rabbit synoviocytes by recombinant retrovirus.