In recent years,the clinical utility of circulating tumor cells (CTCs)analysis from peripheral blood has undoubtedly become a popular topic in the field of precision medicine.CTCs analysis is a noninvasive,easily obtained and highly repeatable testing approach,and could be carried out in a real-time manner,which has never failed to surprise us with great progression in the detection of early disease,assessing prognosis,treatment response monitoring and individualized therapeutic direction in breast cancer.

Objective:To prepare DEET-ethylcellulose microsphere(DEET-EC) and observe its properties to retard volatilization of DEET. Methods: DEET-EC was prepared with solvent evaporation method. DEET was dissolved in CH 2Cl 2 with EC served as dispersed phase and 1% PVA water solution as continuous phase. The dispersed phase was added into the continuous phase with stirring rate at 1 000 r/min. The stirring rate was changed into 600 r/min after 30 min, and was kept until CH 2Cl 2 was entirely volatilized. After being watered, precipitated and lyophilized for 12 h, DEET-EC was derived, and the shape was observed with electron microscope. The particle size distribution was detected in 500 microspheres with optical microscope. HPLC method was established to determine the embedding ratio and loaded ratio of DEET-EC microsphere. Chromatograph conditions: Diamonsil ODS column (150 mm?4.6 mm), CH 3OH∶H 2O=65∶35 as mobile phase at 1 ml/min, the detected wavelength 210 nm. Results: The DEET-EC was egg-white and had spherical shape. Almost 90% of the MS distributed in 30-70 ?m, while ( ar ) and ( v ) were 49.6 ?m and 51.2 ?m, respectively. The loading ratio was 18.7% and the embedding ratio was 56.1%(n=6). Conclusion: The solvent evaporation method is convenient and simple to prepare DEET-EC microsphere.

Objective To investigate the value of MRI for the preoperative diagnosis of breast cancer.Method 45 patients with breast mass were evaluated by MRI preoperatively, 17 cases were further inspected by dynamic MRI, and the time intensity curves were analyzed.Results Postoperative pathology revealed carcinoma in 24 cases, and benign lesions in 21. The correct preoperative diagnosis rate of MRI for breast cancer was 96%(23/24), and its false-positive rate was 2%(1/45).Conclusions MR imaging of breast has practical value in preoperative diagnosis for breast cancer.

Objective To study the effects of nicotine on GDNF and dopamine content in striatum of Parkinson's disease (PD) rats.Methods 6-OHDA was stereoscopically injected to right side of the mesencephic ventral tegmental area (VTA) and substantia nigra pars compacta (SNpc). Nicotine of different dosages was intraperitoneally injected into PD rats.With biochemical,immunohistochemical techniques, we observed the changes of GDNF expression and dopamine content in striatum.Results Nicotine was intraperitoneally injected into PD rat models before or after the operation,striatum GDNF expression and DA content had significant improvement as compared with PD group (P

Asian Continental Ancestry Group ; China ; epidemiology ; Fatty Liver ; epidemiology ; Humans ; Hyperlipidemias ; epidemiology

Adult ; Female ; Humans ; Male ; Middle Aged ; Pharyngeal Neoplasms ; Young Adult

Objective To investigate the effect of tongue tie corrective surgery on the development of language in children with short lingual frenulum.Methods 94 children with short lingual frenulum were selected,and under the permission of the patients'family they were randomly divided into two groups.The treatment group received ankylotomy and language training.The control group was only given language training.The clinical efficacy,improvement time of pronunciation and adverse reactions were compared between the two groups.Results The total effective rate of the treatment group was 91.49％,which was significantly higher than 72.34％ of the control group (x2 =5.816,P ＜0.05).The improvement time of pronunciation in the treatment group was significantly shorter than the control group(t =21.250,P ＜ 0.01).There were no serious adverse reactions observed in two groups.Conclusion Tongue tie correction surgery can significantly affect the language development in children with short lingual frenulum,the improvement time of pronunciation is short,which is worthy of clinical application.

Objective To discuss the causes and the prevention measures of the complications occurred after interventional therapy for different type of Budd-Chiari syndrome (BCS). Methods Based on the type of BCS, the corresponding interventional management was adopted in 204 patients with BCS. The interventional procedures included PTA and stent placement of inferior vena cava (IVC), percutaneous transhepatic recanalization and dilation (PTRD) of hepatic vein, percutaneous transjugular or transinferior vena cava recanalization, dilation and stent placement of hepatic vein and transjugular intrahepatic portal-systemic stenting shunt (TIPSS). Results The successful rate of interventional therapy was 95.5% (21 / 22) for type Ia, 81.8% (9 / 11) for type Ib, 97.3% (109 / 112) for type IIa, 92.9% (13 / 14) for type IIb, 88.9% (8 / 9) for type Ⅲa, 100% (2 / 2) type Ⅲb, 92% (23 / 25) for type Ⅳa and 88.9% (8 / 9) for type Ⅳb BCS. The main complications occurred during or after the operation included acute cardiac insufficiency (n = 2), pulmonary arterial embolization (n = 4), disseminated intravascular coagulation (n = 1), extravasation of contrast medium (n = 3), arrhythmia (n = 2), and cardiac tamponade (n = 1). Conclusion Interventional therapy is simple, safe and effective for the treatment of BCS, but its indications should be strictly considered and all kinds of effective prevention measures should be taken to avoid or to reduce the possible complications.

BACKGROUND:Previous research has proved good therapeutic effect on the acute hepatic failure by the treatment of polylactic acid-O-carboxymethylated chitosan(PLA-O-CMC) nanoparticles-cultured porcine hepatocytes embedded in type I collagen.OBJECTIVE:To investigate the effect of PLA-O-CMC nanoparticles on immunological rejection in intraperitoneal porcine hepatocyte xenotransplantation for treatment of acute liver failure rats.DESIGN,TIME AND SETTING:Randomized control animal experiments were carried out at the Institute of Hepatobiliary Surgery and Laboratory of Nerve Regeneration in Nantong University from May 2005 to May 2006.MATERIALS:In situ recirculating collagenase perfusion method was used to isolate porcine hepatocytes.64 SD rats were intraperitoneally injected with D-galactosamine to induce acute hepatic failure models.METHODS:Totally 64 rats were divided into four groups:Model group received no intervention;Nano-collagen hepatocytes group was transplanted with PLA-O-CMC nanoparticles-cultured porcine hepatocytes embedded in collagen gel,which was then wrapped up using greater omentum;Porcine hepatocytes embedded in collagen gel(collagen hepatocytes group) and free porcine hepatocytes suspension(pure hepatocytes group) were transplanted into peritoneal cavity of SD rats,respectively.At 48 hours following the modeling,the porcine hepatocytes transplantations were performed and the number of transplanted cells was 5.0?107 hepatocytes in all groups.MAIN OUTCOME MEASURES:The serum interleukin-2,interferon-?,IgG and IgM levels of rats were determined at days 1,2,3,5 and 7.The pathological changes of transplants were observed under microscope at days 1,3 and 7.RESULTS:There were no significant differences in serum interleukin-2 and interferon-? levels of all groups during 7 days post-transplantation.Serum IgG levels increased with a peak at day 3 after hepatocytes transplantation,and then began to decline.Serum IgG levels in nano-collagen hepatocytes group were lower than those in collagen hepatocytes group and pure hepatocytes group at days 2 and 3(P

Objective To explore the application value of cellular automata(CA)in simulating the epidemic spread of out-break of influenza A(H1N1).Methods The publications regarding influenza A(H1N1)from January 2009 to March 2015 were collected from the China National Knowledge Infrastructure(CNKI),epidemiological data of H1N1 were retrieved ac-cording to inclusion criteria,the Matlab 7.0 software was adopted to construct CA model for simulating and analyzing the epidemic of H1N1 occurred in a university in Chongqing between October 12 and November 20,2009.Results There were a total of 17 820 students in this university,the epidemic of influenza lasted 40 days in 2009;When the parameter,the ef-fective infection rate was 0.04,the model of CA fit well,and gave estimate for basic reproduction number (R0 )1.202. Conclusion CA has certain reliability in simulating epidemics of airborne infectious diseases,it can provide reference for the prevention and control of disease.