The aim of this study is to investigate the apoptotic inhibition and its molecular mechanism of dexamethasone (DEX) acting on cisplatin (CDDP)-induced apoptosis of human lung adenocarcinoma cell SPC-A1; SPC-A1 cells were pre-cultured in vitro for 24 hours with DEX in different concentrations and then CDDP was added in different concentrations for culturing for further 48 hours. The survival rates of the cells were determined by MTT. The expression of serum/glucocorticoid-induced kinase (SGK-1) and mitogen-activated protein kinase phosphatase-1 (MKP-1) in SPC-A1 cells after being cultured by 1 micromol/l DEX at different time was detected by semi-quantitative RT-PCR technology. The expression of glucocorticoid receptor (GR) in SPC-A1 cells was measured by immunohistochemistry (IHC) with biotin-labeled anti-GR. The results of MTT showed that SPC-A1 cells had resistance to CDDP-induced apoptosis with pre-cultured DEX and the resistance intensity presented DEX concentration-dependent. The expressing quantity of SGK-1 in SPC-A1 cells stimulated by DEX could be elevated and increased with intention of time, but the express of MKP-1 was not detected. Up-regulated expression of GR in SPC-A1 cells stimulated by DEX was detected by IHC. The number of cells expressing GR in SPC-A1 cells was significantly higher than that in the control group. The results showed that DEX inhibited apoptosis of SPC-A1 cells induced by CDDP. The possible molecular mechanism is that elevated expression of GR induced by DEX up-regulates the expression of SGK-1 which locates at the downstream of anti-apoptosis pathway. The apoptosis resistance of SPC-A1 cells may account for all above the factors.
Adenocarcinoma ; pathology ; Apoptosis ; drug effects ; Cell Line, Tumor ; Cisplatin ; pharmacology ; Dexamethasone ; pharmacology ; Dual Specificity Phosphatase 1 ; metabolism ; Humans ; Immediate-Early Proteins ; metabolism ; Lung Neoplasms ; pathology ; Protein-Serine-Threonine Kinases ; metabolism ; Receptors, Glucocorticoid ; metabolism ; Up-Regulation

Objective To investigate the effect of empowerment education on self-management in patients with diabetes mellitus. Methods A total of 128 patients with diabetes mellitus who met the criteria of WHO in 1999 were selected and divided into the control group(62 cases)and the observation group(66 cases). The control group accepted the traditional nursing methods, and the observation group accepted the empowerment education model. FBG, PBG, HbA1c and Diabetes Management Self-efficacy Scale (C-DMSES) were compared between two groups of patients after 3 months and 6 months. Results After the intervention with the empowerment education model, the patient′s FBG, PBG, HBA1c of the control group were (7.69±4.21) mmol/L, (11.78±3.21) mmol/L, (7.95±1.46)%three months after discharge, and (7.21 ± 3.19) mmol/L, (10.48 ± 4.65) mmol/L, (9.15 ± 2.36)% six months after discharge; while in the observation group, FBG, PBG, HBA1c were (6.23 ± 3.59) mmol/L,(9.32 ± 4.06) mmol/L, (6.69 ± 1.10)%three months after discharge, and (6.15 ± 1.89) mmol/L,(7.33 ± 2.10) mmol/L,(6.02 ± 2.17)%. The differences between two groups were significant (t=2.012-5.196, P<0.05). C-DMSES scores of the control group were 103.48 ± 18.41, 134.38 ± 13.01 on admission and six months after discharge, while in the observation they were 102.83 ± 10.91,157.64 ± 19.56. The differences between two groups were significant (t=3.151, P<0.05). Conclusions Empowerment education model can improve the ability of self-management of diabetes patients, improve self-efficacy to improve their own ability to control blood glucose.

Objective To compare the long-term outcome between breast-conserving therapy with mastectomy therapy for early stage invasive breast cancer through a Meta analysis of the randomized controlled trials published worldwide.Methods Cochrane systematic evaluation was used to search through Cochrane libraries of clinical comparative trials,PubMed,Embase,Cancer Lit,and so on.The quality of literatures was independently evaluated and cross-checked by two evaluators,indicators for assessment including death number at the end of follow-up,locoregional and total recurrence.The results were analyzed with RevMan 4.2.2 software.Results Six articles were involved in the Meta analysis with total 3 933 patients.No statistical difference was found in the death at the end of follow-up between breast-conserving therapy group and mastectomy therapy group(OR=1.05,95% CI=0.93-1.19,P=0.45).Locoregional and total recurrence rate of breast-conserving therapy group were statistical higher than those in mastectomy therapy group(OR=1.64,95% CI:1.10-2.44,P=0.01;OR=1.42,95% CI:1.22-1.64,P

Objective To evaluate risk factors for surgical site infection(SSI) in patients with thoracolumbar fracture,and provide basis for making prevention and control measures.Methods Patients with thoracolumbar fractures operation in the department of spinal surgery of a hospital between January 2010 and March 2015 were monitored through information monitoring system,on-site visit,and telephone follow-up,incidence of SSI was investigated,risk factors were performed univariate analysis.Results A total of 326 patients undergoing thoracolumbar fracture operation were monitored,15(4.60％) had SSI.SSI were high in patients≥60 years old,underwent operation at nonlaminar air flow operation room,didn't receive antimicrobial prophylaxis 30 minutes prior to operation,preoperative hospital stay ＞3 days,operation duration ＞3 hours,as well as with diabetes mellitus and chronic respiratory disease (all P＜ 0.01).Conclusion Risk factors for SSI in patients undergoing thoracolumbar fractures operation were patients with older age,receive operation at non-laminar air flow operation room,didn't receive antimicrobial prophylaxis,long length of preoperative hospital stay and operation duration,as well as combined with diabetes mellitus and chronic respiratory disease,preventive measures should be taken to reduce the incidence of infection.

Coronary Vessels ; Humans ; Pulmonary Artery

Traditional Chinese medicine (TCM) is a great knowledge base that has been developed through thousands of years and has been accumulated a wealth of experience in diagnosis and treatment.TCM has advanced concepts,but lag behind the use of modern technology and means.With the progress of modern science and technology,the combination of TCM theory and moderm science and technology promotes the development of TCM engineering.TCM has been a breakthrough in diagnostic techniques and clinical treatment,especially modern TCM instruments.At present,diagnostic instruments of TCM mainly depend on pulse and tongue device,and gradually develop to four diagnostic comprehensive analysis platform.Therapeutic instruments of TCM include electroacupuncture device,meridian instrument,laser treatment device and magnetic therapy instrument.This article focuses on the current status,problems and trend on TCM diagnostic and therapeutic instruments,which mainly include diagnostic instruments,clinical treatment instruments,Chinese medical instruments.

OBJECTIVE: To detect the levels of aquaglyceroporin 9 (AQP9) mRNA expression, AQP9 and p38 proteins and their phosphorylation in HepG2 and L-02 cells treated with NaAsO2, and to investigate the association of these expression levels with arsenic intake and the AQP9 phosphorylation mechanism. METHODS: The intracellular arsenic content was determined by inductively coupled plasma mass spectrometry (ICP-MS). Real-time quantitative PCR, Western blotting and immunoprecipitation techniques were used to detect AQP9 mRNA, AQP9 and p38 protein levels and their phosphorylation levels in HepG2 and L-02 cells. SPSS statistical software was used to analyze experimental data. RESULTS: Intracellular arsenic content and intake rate in HepG2 cells were faster than those in L-02 cells. AQP9 mRNA levels in L-02 cells was increased with time within 6 h after NaAsO2 treatment (P<0.05), while no significant change was observed in L-02 cells. Two hours after treatment, AQP9 gene levels in HepG2 cells were all significantly increased at different concentrations of NaAs02. The phosphorylation levels of AQP9 in HepG2 cells were increased with treating time and concentration of NaAsO2. While the phosphorylation levels of AQP9 in L-02 cells significantly increased compared to control at each time point and concentration, but no significant difference was shown between the treatments. p38 phosphorylation levels in both cells were increased with time. Inhibition of p38 activity by SB203580 completely abolished AQP9 protein phosphorylation in L-02 cells, while it had no significant effect on HepG2 cells. CONCLUSION: AQP9 expression and phosphorylation levels may play an important role in regulating arsenic influx; regulation mechanism of AQP9 phosphorylation may be different in different cells. Copyright 2012 by the Chinese Pharmaceutical Association.

Orthopedics virtual surgery system is a rapidly developing domain in recent years,and has exhibited a promising application.Currently,softwares about orthopedics virtual surgery system have already been applied in medical clinic and education.This paper reviewed present situation of this system,introduced some key technology such as medical image segmentation,registration and fusion,3D visualization and 3D interactive,and discussed main application such as orthopedics virtual surgery,surgical protocol,assisting clinical diagnosis,real-time chiri-cal consmlfation,assisting medical education,training and examination,rehabilitation care,prosthetic device,or guidance in surgery.In addition,the development trend of this system is predicted.With development of orthopedics virtual surgery system,the time of surgery will be consumedly economized and the risk of surgery will be reduced,greatly benefiting orthopedics.

Transpedicular screw fixation for cervical spine is the strongest spinal posterior fixation that could provide tri-column biomechanical stability,and it has been gradually identified as a quite high value in clinical application.The pedicle of vertebral arch is adjacent to vital neurovascular structures;thereby pedicle screw fixation in the cervical spine has been considered as a potential risk of injury and been limited in clinics.This study aims to review the studies on the current situation and progress of pedicle screw fixation techniques in lower cervical disease by investigating cervical pedicle morphology,biomechanical property,transpedicular screw insertion techniques and internal fixation system,computer assisted image-guided navigation,the indication,contraindication and complications of pedicle screw fixation.The article shows that preoperative careful evaluation for the morphology of the lower cervical pedicles,using kinds of new image guided techniques and meticulous techniques of screw placement in operation are essential in reducing operational risk and offering widely application.

AIM: To study the effects of L-arginine on sebaceous gland hyperplasia in rats. METHODS: 10 mg?kg -1?d -11 of L-arginine was poured into the stomach in Sprague-Dawley rats for 7-14 d. The pathology phenomenon was observed in the rat skin. RESULTS: The backside skin of the male SD rats showed dewiness, greasiness and engrained yellow. The incidence was 100% for 7 d in the male SD rats, 80% in the female SD rats for 14 d, but 100% in the immature female SD rats for 14 d. CONCLUSION: L-arg nine can facilitate the hyperplasia of sebaceous gland in SD rats.