Objective To explore the effect of seminar applied in instruction of Introduction of Nursing Science for advanced vocational nursing students.Methods A total of 246 advanced vocational nursing students admitted in 2013 from class 1 to class 5 were named as the control group,who received traditional teaching method.Meanwhile a total of 252 advanced vocational nursing students admitted in 2013 from class 6 to 10 were set as the experimental group,and were given seminar teaching.We adopted California Critical Thinking Dispositions Inventory Scale,Academic Self-efficacy Scale and an anonymous questionnaire for teaching satisfaction.Theoretical examination was used to be final evaluation.Results The scores of theoretical examination in the experimental group were higher than that of the control group.The scores of critical thinking ability and academic self-efficacy of the experimental group were higher than those of the control group,with a significant difference.The result of the anonymous questionnaire showed that the experimental group showed a higher satisfaction degree with seminar teaching method.Conclusions Seminar teaching method applied in Introduction of Nursing Science is feasible and effective,which is better than traditional method in improving critical thinking ability,academic self-efficacy and teaching satisfaction.

Objective:To establish a method for the uncertainty evaluation of the determination of benzoic acid. Methods: The content of benzoic acid was determined by acid-base titration. By constructed mathematics model, the source of the measurement uncer-tainty was analyzed, and the uncertainty components were quantized and combined. Results:The expanded uncertainty of benzoic acid was 0. 36% and the results were expressed as(99. 99 ± 0. 36%,k=2). Conclusion:The mathematics model is reasonable and relia-ble,and can be used in the uncertainty evaluation of content measurement of benzoic acid.

AIM:To analyze the clinical significance of axial length, diopter and retinal nerve fiber layer thickness in hyperopia children with anisometropia amblyopia. ·METHODS: From January 2015 to January 2017 in our hospital for treatment, 103 cases, all unilateral, were diagnosed as hyperopia anisometropia amblyopia. The eyes with amblyopia were as experimental group ( 103 eyes), another normal eye as control group (103 eyes). We took the detection with axial length, refraction, foveal thickness, corrected visual acuity, diopter and the average thickness of retinal nerve fiber layer. ·RESULTS: Differences in axial length and diopter and corrected visual acuity were statistically significant between the two groups (P<0. 05). The two groups had no significant difference in retinal nerve fiber layer thickness (P>0. 05). There was statistical significance difference on the foveal thickness (P<0. 05). There was a negative correction correlation between the visual acuity, axial length with foveal and peripapillary nerve fiber layer thickness, but no significant difference in correlation ( P>0. 05 ). The positive correlation between diopter with nerve fiber layer thickness of foveal and around the optic disc were no statistically significant difference (P>0. 05). ·CONCLUSION:Retinal thickness of the fovea in the eye with hyperopic anisometropia amblyopia were thicker than those in normal eyes;the nerve fiber layer of around the optic disc was not significantly different between the amblyopic eyes and contralateral eyes. The refraction and axial length had no significant correlation with optic nerve fiber layer and macular foveal thickness.

Objective To study the copulation of the abnormal level of maternal serum-free β-human chorionic gonadotropin ( β-hCG ) during second-trimester pregnancy and adverse pregnancy outcome.Methods Two hundred and nine pregnant women in second-trimester pregnancy were detected serum β-hCG and followed up ti11 delivery.The correlation of the abnormal level of serum β-hCG and adverse pregnancy outcome were observed.Results In 209 pregnant women,the level of serum β -hCG of 105 cases were over standard [(84.9 ± 11.6) kU/L](over standard group),while the level of the rest 104 cases were (18.6 ± 1.3) kU/L within normal range (normal group).The incidence rate of gestational hypertension,premature deliwery,premature rupture of membranes and suffocation of new born infants in over standard group were all higher than those in normal group [14.3％(15/105 ) vs.0,18.1％(19/105) vs.2.9％ (3/104),11.4％(12/105) vs.0,29.5％(31/105) vs.0,P＜0.05].Conclusions The abnormality of serum β -hCG of women in second-trimester pregnancy can reflect the placenta dysfunction and it is a signal of adverse pregnancy outcome.Therefore,detecting the level of serum β -hCG is useful for preventing adverse pregnancy outcome.

Establishing the assessment methods of childhood asthma is highlighted in asthmatic prevention and treatment.Due to the factors such as age,genotypic environment,pathogenesis and treatment,the assessment methods existed have some defects and cant objectively reflect the situation of asthma disease and airway inflammation.The assessment methods including symptom scores,pulmonary function test,airway responsiveness detection and fractional concentration of exhaled nitric oxide are described in this review.The principles,advantages,disadvantages,application ways and so on are discussed in detail,to improve the rationality of the application in these methods in order to make them play a greater role in asthmatic diagnosis,disease detection,medication guild and prognosis estimation,and explore a more effective and simple assessment way in children asthma.

Objective To determine the best time to operate on elderly patients with acute severe cholangitis (ACST).Method The time to operate was analysed in 36 elderly patients with ACST.Result With active preoperative anti-shock treatment,the mortality rates for emergency surgery within and after 8 hours were 8.3％ and 33.3％,respectively.Conclusion Early operation for elderly patients with ACST markedly reduced mortality.

ObjectiveTo observe the effect of toosendanin on proliferation and apoptosis of human hepatoma cell line HepG2 cells,and to study the mechanism.MethodsHuman hepatocellular carcinoma HepG2 cells were randomly divided into control group(group A) does not add any medication,the observation group(group B) joined TSN 80mmol/ml,cultured cells for 24,48,72 hours.HepG2 cells proliferation inhibition rate was measured by MTT; Hepatocellular carcinoma HepG2 cell cycle and apoptosis rate was deteded by flow cytometry.ResultsTECAN determination revealed two groups of hepatocellular carcinoma cell proliferation had statictical significance ( x2 =5.33,P ＜0.05 ) ;flow cytometry revealed two groups S,G0/G1,M/G2 phase of the cell rate ( t =6.31,6.26,6.56,all P ＜0.05 ),apoptosis rate had significant difference ( x2 =6.15,P ＜ 0.05 ).ConclusionToosendanin could obviously inhibit the proliferation of HepG2 cells,and induce apoptosis of tumor cells.

Objective Discusses TT3,FT3,TT4,FT4 and TSH the value which examines in the thyroid gland disease patient;analysis thyroid gland disease patient TT3,FT3,TT4,FT4 and TSH change situation.Methods analyzes my hospital 200 example thyroid gland disease patients,and selects the normal adult 50 examples to take the control group.analytic method to each group of examination thyroid function,and carries on the comparison to the group various targets'examination value.Results primary hyperthyroidism group T3,T4,FT3,FT4 were higher than normally,the hypothyroidism group T3,T4,Fr3,FT4 to be lower than normally.The TSH content primary hyperthyroidism group was lower than normally,the hypothyroidism group to be higher than normally obviously.Compares its difference with the control group to have statistics significance.hyperthyroidism group its diagnosis coincidence rate FT3 was 96%,TSH was 96%,T3 was 92%,FT4 was 90%,T4 was 88%.The hypothyroidism,the diagnosis coincidence rate respectively was:TSH was 100%,FT4 was 93%,T4 was 90%,FT3 was 77%,T3 was 70%.Conclusion FT3,T3,TSH the significance are big in the hyperthyroidism diagnosis;FT4,T4,TSH the significance are big in the hypothyroidism diagnosis.

Objective To investigate the efficacy and safety of uterine brace suture technique in the treatment of postpartum hemorrhage caused by uterine inertia.Methods 63 cases of postpartum hemorrhage of cesarean section were divided into two groups.33 patients treated with uterine brace suture technique severd as the research group.30 patients treated with traditional technique for postpartum hemorrhage of cesarean section were recruited as the control group.Operation time,hemorrhage volume,puerperal morbidity and therapeutic efficacy between the two groups were compared.Results Operation time was(53.31 4±10.61)min in the research group,and(76.16±13.73)rain in the control group,respectively.There were(601.00±162.36)ml of hemorrhage volume in the research group,and(1 063.33±320.10)ml in the control group,respectively.No case with hysterectomy in the research group,however 5 cases were treated with hysterectomy in the control group.There were 2 cases of puerperal morbidity in the research group,and 9 cases in the control group,respectively.The differences were statistically significant in operation time,hemorrhage volume,puerperal morbidity and therapeutic efficacy between the research group and the control group(t=7.428,7.121,χ2=4.698,all P＜0.05).Conclusion Uterine brace suture technique is one of effective treatment for postmnurn hemorrhage caused by uterine inertia,particularly useful because of its simplicity of application and relative safety.It Was worthy to be popularized in primary hospitals.

Caveolin-1 is a prominent member of the caveolin family.Caveolin-1 may have closely correlation with some carcinoma' s origination,development,infihration,transference and drug tolerance of tumor cell.But this function has its specificity of cell type and organ.Caveolin-1 can have different functions in formation and progress of tumor cell.Because different thyroid tumors have different structures and growth patterns ,as well as different biological behavior.Expressions of Caveolin-1 have significant differences in thyroid carcinoma with different histological type and differentiation grade.Research on expression of caveolin-1 in different types of thyroid carcinomas and its physiological characteristics play roles in evaluating prognosis of patient with thyroid carcinoma.