Increased intracranial pressure is one of the most severe complications with significant mortality in children,so early diagnosis and treatment of this disorder is critical to save the patient's life.This article reviews etiologies,pathophysiology,and general principles of diagnosis and management of increased intracranial pressure.Based on primary diseases and clinical presentations,the goal of therapeutic strategy is to decrease intracranial pressure,avoid neurologic sequelae,and improve the outcome in patients.

Integrative medicine is the effective combination of dual diagnosis of Chinese medicine and western medicine.The combination can broaden the scope of clinical diagnosis and combine the local pathological changes with the overall response of the human disease.It can add a more comprehensive understanding of diseases for people.Clinically treated with combination of Chinese medicine and western medicine can achieve the complement of advantages.It is significant.Because the theory system of Chinese medicine compared with western medicine is different and the theory system of combination is not practical and standardized.The methods of clinical diagnosis and treatment are also different.So there are still some problems and deficiencies in the integrative medicine.In this study,through extensive literature research,as well as the clinical investigation in some integrative medicine hospitals and the collection of related clinical data,we compared and analyzed the academic ideas and different features of Chinese medicine and western medicine,analysed the status and the insufficience of current clinical integrative medicine.Finally,we put forward a series of effective strategies and methods for how to combine with integrative medicine in a specific,reasonable and effective way.

Influenza virus is a continuous and severe global threat to mankind.The continuously re-emerging disease gives rise to thousands of deaths and enormous economic losses each year,which emphasizes the urgency and necessity to develop high-quality influenza vaccines in a safer,more efficient and economic way.The influenza subunit and VLP vaccines,taking the advantage of recombinant DNA technologies and expression system platforms,can be produced in such an ideal way.This review summarized the recent advancements in the research and development of influenza subunit and VLP vaccines based on the recombinant expression of hemagglutinin antigen (HA),neuraminidase antigen (NA),Matrix 2 protein (M2) and nucleocapsid protein (NP).It would help to get insight into the current stage of influenza vaccines,and suggest the future design and development of novel influenza vaccines.

Dental Implantation ; methods ; Humans ; Time Factors

Objective:To investigate the distribution of TNF microsatellite polymorphisms in Chinese Han population of Hubei province Methods:DNA samples were extracted from 164 unrelated healthy individuals’EDTA blood TNF microsatellite alleles were typed using PCR technique,followed by High Voltage denaturing PAGE,with silver staining At the same time,the PCR products were cloned and sequenced Results:Detected seven alleles and eleven kinds of genotypes at the TNFb locus;four alleles and seven kinds of genotypes at the TNFe locus The polymorphism information contents (PIC) were 0 67 and 0 33 respectively No deviation from Hardy Weinberg equilibrium were observed Statistical analysis showed,the distribution of TNFb and TNFe alleles in Chinese Han population were significantly different from that in European or in American Caucasian(P

OBJECTIVE: To investigate the effects of puerarin on impaired endothelium-dependent relaxation induced by glycosylated bovine serum albumin (GBSA) in rabbit thoracic aorta and its mechanisms. METHODS: The rings were incubated with GBSA for 30 min to induce endothelial dysfunction, and with puerarin(0.25, 0.5 and 1 g · L-1), A-nitro-L-arginine methyl ester (L-NAME, 30 νmol · L-1), and indomethacin(10 μmol · L-1) to investigate the protective effect of puerarin on impaired vascular endothelial function elicited by GBSA, and the effect of L-NAME and indomethacin on the protective effect of puerarin. Moreover, the content of nitric oxide (NO) and malonaldehyde (MDA) and the activity of superoxide dismutase(SOD) in the rings were measured. RESULTS: Exposure of aortic rings to GBSA (200 mg · L-1) for 30 min resulted in a significant inhibition of endothelium-dependent relaxation, but had no affect on endothelium-independent relaxation. GBSA significantly decreased the level of NO and the activity of SOD but hugely increased the content of MDA in vascular tissues. Pre-incubation of aortic rings with puerarin markedly attenuated the inhibition of endothelium-dependent relaxation induced by GBSA. This protective effect of puerarin (1 g · L-1) was partially inhibited by L-NAME but not by indomethacin. Puerarin obviously increased NO level and SOD activity but evidently decreased MDA content in rings. CONCLUSION: Puerarin can protect against vascular endothelial dysfunction caused by GBSA and its mechanisms may be related to enhancing NO synthesis and its anti-oxidation.

Objective To investigate the abnormality of CD4 and CD8 subsets of T lymphocytes in the peripheral blood of systemic lupus erythematosus patients. Methods The CD4 + and CD8 + T lymphocytes from 20 SLE patients and 10 healthy controls were separated using magnetic activated cell sorting (MACS). Results The numbers of CD4 + T cells in the peripheral blood and CD4 +/CD8 + ratio of SLE patients were significantly lower than those in healthy volunteers(CD4,P=0.001;CD4/CD8 ratio,P=0.046). Conclusion There exist abnormalities in the CD4 and CD8 subsets of T lymphocytes in the peripheral blood of SLE,especially the decreased numbers of CD4 + T cells.

Objective:To study the effect of retinoic acid (RA) and basic fibroblast growth factor (bFGF) on neural stem cells’(NSCs) proliferation and differentiation from new born rats’ pallium. Method: NSCs were isolated from the brains of new born Sprague-Dawley rats’ pallium, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on survival and proliferation of cells were determined by MTT assay. The effects of bFGF and RA on differentiation of NSCs were observed. Results: MTT assay indicated the proliferation of cells in bFGF group, bFGF+RA group and RA group was continually increased, highest in bFGF group. The percentage of neurons differentiated from NSCs in RA group was 2 or 3 times that in bFGF group and control group . Conclusion: bFGF is important to the proliferation and long-term living of NSCs. It can prohibit the differentiation of NSCs. RA can counteract the effects of bFGF and also promote NSCs to differentiate into neurons in vitro.

Objective To investigate the effect of photodynamic therapy with chlorin e6 (Ce6) and 5-aminolevulinic acid (5-ALA) on mouse model of malignant melanoma. Methods The mouse model of malignant melanoma was established by injecting 0.1 ml A-375 cells (about 2?10 6 cells) under the right hind leg of BALB/c-6 mice,and that the yellowish white node appears at injection site proves the successful model. Twenty-four of 27 successful mouse models were irradiated at the tumor site with semiconductor laser (wavelength 652 nm) with a total dose of 100 J/cm 2 . Before laser exposure,the mice were treated with 10% 5-ALA by topical compress for 2 h or 7.5 mg/kg Ce6 by intraperitoneal injection for 1 hour or 5-ALA topical application combined with intraperitoneal injection of Ce6 (n=6 in each group). Another six mice as control only underwent PDT. One week after PDT,the mice were killed,the tumor mass was peeled off and weighed,whether the metastasis occurred or not was detected,and the tumor,liver,spleen,lung,kidney were sent to histopathological examination. Results The tumor weight in 5-ALA group,Ce6 group,and the combined group had significant difference as compared with control group (P0.05). The dehydration and scab formation and necrosis could be seen in tumor sites at 1 week after PDT. The cell collapse and necrosis,subdermal thrombosis and cell outline clouding could be observed by histopathological examination. Metastasis of melanoma were found in 5-ALA group,Ce6 group,and the combined group. Conclusion PDT with Ce6 and 5-ALA could kill the malignant melanoma effectively in animal experiment but could not affect the metastasis of melanoma.

Objective To construct a library of long serial analysis of gene expression (LongSAGE) and investigate the characteristics of gene expression in the yeast phase of C. albicans. Methods A LongSAGE library was constructed with long serial analysis of gene expression (LongSAGE). Results A total of 17 773 tags, representing specific 7 333 transcripts, were extracted from 1 000 sequence files in the yeast phase of C. albicans. Tags matching single gene accounted for 16.65%, tags matching multiple genes accounted for 6.59%, tags for hypothetical protein accounted for 16.27%, tags for genome and cosmid accounted for 1.64%, and novel tags accounted for 58.86%. Eleven tags in 33 tags whose expression frequencies exceeded 35 may be new expression sequences. Conclusion LongSAGE is not only a powerful method in investigating the gene expression profiling, allowing the analysis of the expression of thousands of transcripts in a quantitative manner without prior sequence information, but also in finding new genes.