1.Investigation of the role of HIF-1α and VEGF in women with missed abortion
Yanli LI ; Lili YANG ; Han GAO ; Yichong YUAN ; Faxia ZHU ; Bin YAN ; Xufeng WU
China Modern Doctor 2014;(35):127-129,132
Objective To investigate the association of HIF-1αand VEGF signaling in missed abortion. Methods Fifty patients with missed abortion were selected as study group, and fifty early pregnant women who underwent artificial abortion surgery were selected as control group. The expressions of HIF-1α and VEGF were measured by immunohis-tochemistry. Results The expressive rate of VEGF was 100%in the control group and 66% in the study group. The ex-pressive rate of HIF-1α was 42% in the control group and 76% in the study group. Compared with the control group, the expression of VEGF was statistically reduced(P<0.05) while the level of HIF-1α was significantly up-regulated(P<0.05)in the study group. In the control group, HIF-1αwas positively correlated with VEGF(r=0.387,P<0.05), while in the study group HIF-1α was close negatively correlated with VEGF(r=-0.298,P>0.05). Conclusion The decrease of VEGF and increase of HIF-1αin villous trophoblastic cells may be signals of occurrence of missed abortion.
2.Value of tumor-associated CD66b-positive neutrophils for the prediction of the recur-rence of stage Ib and IIa cervical cancer
YAN BIN ; CHEN HUIJUN ; GAO HAN ; TAN WENFU ; CAI HONGNING ; ZHOU ZHIGANG ; DAI XUAN ; ZHU FAXIA ; XIONG YUJING
Chinese Journal of Clinical Oncology 2017;44(21):1071-1075
Objective:To investigate the prognostic effect of polymorphnuclear neutrophil (PMN) in cervical cancer. Methods:Patients (n=92) who underwent curative surgery for the treatment of stage Ib and IIa cervical cancer according to the International Federation of Gynecology and Obstetrics (FIGO) were assessed to determine their tumor-infiltrating CD66b-positive neutrophils through immuno-histochemistry. Assessment results were then analyzed to identify their correlation with recurrence-free survival (RFS) as an end point. Kaplan-Meier method was used for survival curve analysis, and a Cox proportional hazard model was utilized for univariate and multi-variate analyses. Results:The RFS of the group with a density of CD66b-positive neutrophils above the median in cervical cancer tis-sues was significantly shorter than that of the group with a density of CD66b-positive neutrophils below the median (P=0.001). Univari-ate and multivariate analyses revealed adenocarcinoma (HR=3.020;95%CI=1.340-6.805;P=0.008), lymph node metastasis (HR=2.450;95%CI=1.065-5.637;P=0.035), and high neutrophil density (HR=2.866;95%CI=1.274-46.447;P=0.011) as independent prognostic fac-tors of short RFS. Conclusion:The increasing number of tumor-infiltrating neutrophils in cervical cancer tissues was correlated with short RFS of patients with cervical cancer.