1.Identification of α-glucosidase inhibitors from Clinacanthus nutans leaf extract using liquid chromatography-mass spectrometry-based metabolomics and protein-ligand interaction with molecular docking
Murugesu SUGANYA ; Ibrahim ZALIKHA ; Ahmed Uddin QAMAR ; Uzir Fathamah BISHA ; Nik Idris Nik Yusoff ; Perumal VIKNESWARI ; Abas FARIDAH ; Shaari KHOZIRAH ; Khatib ALFI
Journal of Pharmaceutical Analysis 2019;9(2):91-99
The present study used in vitro and in silico techniques, as well as the metabolomics approach to char-acterise α-glucosidase inhibitors from different fractions of Clinacanthus nutans. C. nutans is a medicinal plant belonging to the Acanthaceae family, and is traditionally used to treat diabetes in Malaysia. n-Hexane, n-hexane: ethyl acetate (1:1, v/v), ethyl acetate, ethyl acetate: methanol (1:1, v/v), and methanol fractions were obtained via partitioning of the 80% methanolic crude extract. The in vitro α-glucosidase inhibitory activity was analyzed using all the fractions collected, followed by profiling of the metabolites using liquid chromatography combined with mass spectrometry. The partial least square (PLS) statistical model was developed using the SIMCA P +14.0 software and the following four inhibitors were obtained:(1) 4,6,8-Megastigmatrien-3-one; (2) N-Isobutyl-2-nonen-6,8-diynamide; (3) 1′,2′-bis(acetyloxy)-3′,4′-didehydro-2′-hydro-β, ψ-carotene; and (4) 22-acetate-3-hydroxy-21-(6-methyl-2,4-octadienoate)-olean-12-en-28-oic acid. The in silico study performed via molecular docking with the crystal structure of yeast isomaltase (PDB code: 3A4A) involved a hydrogen bond and some hydrophobic interactions be-tween the inhibitors and protein. The residues that interacted include ASN259, HID295, LYS156, ARG335, and GLY209 with a hydrogen bond, while TRP15, TYR158, VAL232, HIE280, ALA292, PRO312, LEU313, VAL313, PHE314, ARG315, TYR316, VAL319, and TRP343 with other forms of bonding.
2. Identification of commonly regulated genes in HPV18- and HPV16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one
Felicia PAULRAJ ; Iekhsan OTHMAN ; Sharifah HASSAN ; Rakesh NAIDU ; Faridah ABAS ; Nordin LAJIS ; Faridah ABAS
Asian Pacific Journal of Tropical Biomedicine 2018;7(1):44-51
Objective: To identify commonly regulated genes in HPV-infected HeLa and CaSki cervical cancer cells treated with curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one (MS17) and to explore potential mechanisms that underlie its cytotoxic, anti-proliferative and apoptotic activity. Methods: HeLa and CaSki cells were treated with 2 × EC
3. Identification of commonly regulated protein targets and molecular pathways in PC-3 and DU145 androgen-independent human prostate cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one
Kamini CITALINGAM ; Iekhsan OTHMAN ; Rakesh NAIDU ; Faridah ABAS ; Nordin H. LAJIS ; Faridah ABAS
Asian Pacific Journal of Tropical Biomedicine 2018;8(9):436-445
Objective: To identify mutually regulated proteins in PC-3 and DU145 androgen-independent prostate cancer cell lines treated with 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one (MS17), and to study the molecular pathways that contributed to the anticancer activity of MS17. Methods: PC-3 and DU145 cells were treated with 3 × EC
4.Dietary patterns associated with the risk of type 2 diabetes in women with and without a history of gestational diabetes mellitus: A pilot study
Farah Yasmin Hasbullah ; Barakatun Nisak Mohd Yusof ; Rohana Abdul Ghani ; Geeta Appannah ; Zulfitri &rsquo ; Azuan Mat Daud ; Faridah Abas
Malaysian Journal of Nutrition 2023;29(No.1):89-102
Introduction: There is limited evidence on dietary patterns and the risk of type
2 diabetes (T2D) in women with a history of gestational diabetes mellitus (GDM)
compared to their non-GDM counterparts, especially in the Asian population. The
pilot study investigated dietary patterns in women with a history of GDM (HGDM)
and without a history of GDM (non-HGDM), and the association with T2D risk.
Methods: This comparative cross-sectional study involved 64 women (32 HGDM,
32 non-HGDM). Food intake was assessed using a validated food frequency
questionnaire. Principal component analysis derived the dietary patterns. T2D
risk score was determined using the Finnish Diabetes Risk Score tool. Results:
HGDM group had significantly higher proportion of first-degree family history of
diabetes; higher risk of T2D and better diabetes knowledge; lower gestational weight
gain and postpartum weight retention; and consumed more fast food than nonHGDM. ‘Rice-noodle-pasta-meat’ dietary pattern was significantly associated with
increased T2D risk after adjusting for age (β=0.272, p=0.032). ‘Bread-cereals-fast
food-meat’ dietary pattern was positively and significantly associated with T2D risk
after adjusting for confounders, including age, education level, family history of
diabetes, diabetes knowledge score, gestational weight gain, and postpartum weight
retention (β=0.251, p=0.012). Conclusion: Dietary patterns high in bread, cereals
and cereal products, fast food and meat, as well as rice, noodle, pasta and meat
were associated with an elevated T2D risk. A more extensive study is warranted
to establish the association between dietary patterns and risk of T2D, focusing on
women with a history of GDM.