1.Evaluation of prooxidant-antioxidant balance in in vitro fertilization-conceived mice.
Hamid AHMADI ; Fardin FATHI ; Ashraf MOEINI ; Fardin AMIDI ; Aligholi SOBHANI
Clinical and Experimental Reproductive Medicine 2018;45(2):82-87
OBJECTIVE: Concerns about the safety of assisted reproductive technology (ART) have been raised, as some studies have shown elevated incidence rates of childhood cancer, asthma, allergies, and other diseases in ART-conceived babies. Findings regarding the health of ART-conceived babies are controversial. The present study was conducted to evaluate the prooxidant-antioxidant balance (PAB) in in vitro fertilization (IVF)-conceived mice in comparison to naturally conceived offspring. METHODS: Mice (6–8 weeks) were divided into two groups (IVF-conceived and naturally conceived) matched by sex, age, weight, and litter size. A 1-mL blood sample was taken and the sera were separated. The oxidant-antioxidant balance was evaluated using a fast and reliable PAB assay. The results were expressed as mean±standard deviation. RESULTS: The mean PAB values (HK units) in the IVF-conceived and naturally conceived groups were 59.70±22.30 and 54.70±18.22, respectively (p=0.82). CONCLUSION: Since free radicals contribute to several pathological conditions and antioxidants play an important protective role against oxidative stress, evaluating the oxidant-antioxidant balance is very important. Although the results of this study showed that the quality of the defense mechanism against free radicals was not significantly different between the IVF-conceived and naturally conceived mice, other parameters of metabolic dysfunction need to be measured.
Animals
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Antioxidants
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Asthma
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Fertilization in Vitro
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Free Radicals
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Hypersensitivity
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In Vitro Techniques*
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Incidence
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Litter Size
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Mice*
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Oxidative Stress
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Reproductive Techniques, Assisted
2.The expressions of stem cell markers: Oct4, Nanog, Sox2, nucleostemin, Bmi, Zfx, Tcl1, Tbx3, Dppa4, and Esrrb in bladder, colon, and prostate cancer, and certain cancer cell lines.
Sabrieh AMINI ; Fardin FATHI ; Jafar MOBALEGI ; Heshmatollah SOFIMAJIDPOUR ; Tayyeb GHADIMI
Anatomy & Cell Biology 2014;47(1):1-11
Uncontrolled self-renewal plays a direct function in the progression of different types of carcinomas. The same molecular pathway that manages self-renewal in normal stem cells also seems to manage cancer stem cells. Here, we examine the expressions of self-renewal regulatory factors Oct4, Nanog, Sox2, nucleostemin, Zfx, Esrrb, Tcl1, Tbx3, and Dppa4 in tissue samples of colon, prostate, and bladder carcinomas as well as cancer cell lines HT-29, Caco-2, HT-1376, LNCaP, and HepG2. We used reverse transcriptase polymerase chain reaction to examine expressions of the above mentioned regulatory factors in cancer cell lines HT-29, Caco-2, HT-1376, LNCaP, and HepG2 and in 20 tumor tissue samples. Total RNA was isolated by the ISOGEN method. RNA integrity was checked by agarose gel electrophoresis and spectrophotometry. Expressions of Oct4 and nucleostemin at the protein level were determined by immunocytochemistry. A significant relationship was found between tumor grade and self-renewal gene expression. Expressions of stem cell specific marker genes were detected in all examined cancer cell lines, in 40% to 100% of bladder cancer samples, and in 60% to 100% of colon and prostate cancer samples. Oct4 expressed in 100% of tumor tissue samples. Our data show that stem cell markers Oct4, Nanog, Sox2, nucleostemin, Bmi, Zfx, Esrrb, Tcl1, Tbx3, and Dppa4 significantly express in cancer cell lines and cancer tissues. Hence, these markers might be useful as potential tumor markers in the diagnosis and/or prognosis of tumors.
Cell Line*
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Colon*
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Colonic Neoplasms
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Diagnosis
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Electrophoresis, Agar Gel
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Gene Expression
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Immunohistochemistry
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Neoplastic Stem Cells
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Prognosis
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Prostate*
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Prostatic Neoplasms*
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Reverse Transcriptase Polymerase Chain Reaction
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RNA
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Spectrophotometry
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Stem Cells*
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Biomarkers, Tumor
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Urinary Bladder Neoplasms
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Urinary Bladder*