The article introduces a micro colorimetric method of iron nitrate-mercury Ⅱ thiocyanate to quanfitate chloric ion in serum.It is easy to operate, Its linear relationship, exactitude and developing colour stability are all very good. It is suitable for the application of the spectrometer and the auto analysis instrument. The experiment shows that the best wave length 535 nm can be used to dispel effectively the interfering of thc jaundice (hepatogenous) pigment of the hemocytolysis (hematogenous pigment This method makes use of less blood, and it is rapid, exact. So it is suitable for the batch examination examples in clinical biochemistry laboratory. To spread the method the efficacy can be raised effectively.

It is necessary that to investigate the relationship and interaction between medicine and philosophy,under which medical science can be comprehended in a deeper and wider degree by means of the value and inspiration provided of philosophy thinking.For this reson,the article analyzes several major historical stages of medical model of philosophical thinking by historical and logical approach in order to construct the medical philosophy.

Objective To investigate medical college students'attitude and cognition about innovativeness,and then to explore the possible factors that affect students'capability of innovation.Methods To design questionnaire to survey medical students in random sampling method.Results Medical students have more cognition towards education reform,and the method,structure and the cultural environment of talent training system play significant role in innovative talents training project.Conclusion It is needed to construct national medical model college according to the minority regions and the national characteristics for cultivating innovative talents correspondingly.

Objective To estimate the influences of N-acetyl cysteine (NAC) on a chemical hypoxiamimetic agent CoCl2 induced-injury to, and expressions of inflammatory factors by, an immortal human skin keratinocyte line HaCaT. Methods HaCaT cells were treated with CoCl2 of 2000 μmol/L for 4 hours to set up a chemical hypoxia-induced cell model of skin injury. NAC of various concentrations ( 1000, 2000, 3000 μmol/L)was used to pretreat HaCaT cells for 2 hours prior to the establishment of cell model. After these treatments,cell viability was detected by cell counting kit 8 (CCK-8), the levels of interleukin 6 and 8 (IL-6 and -8) and tumor necrosis factor α (TNF-α) in culture supernatant by ELISA kits, mitochondrial membrane potential (MMP) by rhodamine 123 (Rh123) staining and photofluorography, intracellular reduced glutathione (GSH)content by glutathione detection kit. Results An obvious decline was observed in HaCaT cell viability after pretreatment with various concentrations of NAC for 2 hours. The treatment with CoCl2 of 2000 μmol/L for 4 hours induced an elevation in the supernatant levels of IL-6, IL-8 and TNF-α and a decrease in GSH content and MMP, while the pretreatment with NAC for 2 hours retarded the CoCl2-induced increase in IL-6 and IL-8 levels as well as decrease in GSH content and MMP. Conclusion The reactive oxygen species (ROS) scavenger NAC can protect against CoCl2-induced injury to and inflammatory reaction in HaCaT cells, which may be associated with a decrement in oxidative stress.

Aim To explore the effect of chemical hypoxia-mimetic agent,cobalt chloride(CoCl2)on inflammatory reaction in human keratinocytes(HaCat cells).Methods After HaCat cells were treated with CoCl2 at different concentrations to set up a chemical hypoxia-induced cell model of skin injury,cell viability,intracellular reactive oxygen species(ROS),mitochondrial membrane potential(MMP),the levels of both interleukin 6(IL-6)and interleukin 8(IL-8)as well as the expression of heme oxygenase-1(HO-1)were detected.Results The viability of HaCat cells was reduced by CoCl2 at the concentrations from 500 to 3 000 ?mol?L-1,and the higher CoCl2 doses,the lower cell viability was.CoCl2 induced oxidative stress reaction(increasing ROS production and decreasing MMP).CoCl2 induced inflammatory reaction,enhancing the release of IL-6 and IL-8.CoCl2 at concentrations from 1 000 to 3 000 ?mol?L-1 upregulated HO-1 expression in HaCat cells.Conclusion CoCl2 induces not only oxidative stress,but also inflammatory reaction,increasing the release of both IL-6 and IL-8,as well as HO-1 expression.