OBJECTIVEThe plasmid construction and validation of RET point mutation in vitro.METHODS The RET gene exon in the mainland familial medullary thyroid carcinoma family was analized with muon capture two generation sequencing of. In vitro, the RET relative point mutationswere reconstructed in NIH3T3 cells and the expression levels were studied.RESULTSThe corresponding lentiviral plasmids of RET point mutations were successfully obtained after point mutating the wild RET plasmids, it was verified that the target genes were expressed in NIH3T3 cells stably by Western Blot. CONCLUSIONSStable cell lines carrying RET point mutations were reconstructedsuccessfully, which provide a good platform for studying various point mutations.

Objective To understand the incidence and mortality of gastric cancer in middle-aged and elderly population after Helicobacter pylori eradication , and provide theoretical guidance for the prevention of this disease in Luzhou area. Methods A total of 541 patients with Helicobacter pylori eradication from 2019 to 2021 were selected , they were divided into control group and observation group according to whether they developed gastric cancer in later stage, patients with gastric cancer due to Helicobacter pylori infection were selected as positive control group (N=205) . In addition, the expression of tumor markers such as CEA, CA199, CA125 and proliferation-related factor Ki67 were compared among the three groups, binary Logistic regression was used to analyze the risk factors of gastric cancer in the middle-aged and elderly population after Helicobacter pylori eradication. Results Among the 39(7.21%) of 541 patients developed gastric cancer after HP eradication , including 23males and 16females, with no gender difference (P>0.05) .The positive rate of moderate and severe atrophy of gastric mucosa and the ratio of PPI ≥1 year in the observation group were higher than those in the control group (P<0.05) . In addition , serum CEA , CA199 , CA125 levels were the highest in the positive control group , followed by the observation group , Ki67 expression was also the same. Logistic regression analysis showed that PPI ≥1 year (OR=1.642) , gastric mucosal atrophy (OR=1.459) or severe (OR=3.271) and presence of IM (OR= 3.669) were independent risk factors for gastric cancer after Hp eradication. Conclusion The risk of developing gastric cancer was higher in the middle-aged and elderly after Helicobacter pylori eradication , and the risk of developing gastric cancer was higher in patients with moderate or severe atrophy of gastric mucosa , PPI ≥1 year and having IM , gastroscopic screening should be strengthened to prevent gastric cancer..

Objective: To investigate individualized therapeutic strategy for bilateral carotid body tumors. Methods: Clinical data of 16 patients with bilateral carotid body tumor treated from January 2003 to August 2016 were retrospectively studied. Of the 16 patients, 9 were males and 7 were females; 5 were sporadic and 11 were familial; 8 cases were observed, 1 cases was malignant and treated with chemotherapy, and 7 cases were treated with surgery. The treatment course, perioperative complications and clinical efficacy were assessed. Comprehensive evaluation of bilateral carotid body tumors was performed based on the size of bilateral tumor, clinical manifestations, genetic tests and other indicators. Individual treatment strategies included observation, surgery and observation, bilateral surgery, radiotherapy or chemotherapy. Surgical resection of carotid body tumor was unilateral in 3 cases and bilateral in 3 cases; removal of bilateral carotid body tumors plus unilateral jugular bulb in 1 case; and the internal carotid artery was reconstructed with autologous greater saphenous vein in 1 case. Results: All patients were followed up for 3 months to 12 years. There was no patient death during perioperative period. Superior laryngeal nerve injury occurred in 2 case. Baroreceptor failure syndrome occurred in one patient, but it gradually recoverd with medical treatments. Conlusion It is important to identify whether bilateral carotid body tumors are hereditary and to make an individualized therapeutic strategy for each patient with bilateral carotid body tumors, focusing on the improvement in the quality of life of patient.