Objective? To investigate the current situation of lower limb varicose veins among clinical nurses at a ClassⅢ Grade A hospital and analyze its influencing factors, so as to provide a basis for further intervention and improving protective level. Methods? From July 2018 to August 2018, we selected 810 clinical nurses at a ClassⅢ Grade A hospital as subjects by convenience sampling. We collected patients' general information and carried out questionnaire survey with the self-designed lower limb varicose veins questionnaire for nurses. Receiver operating characteristic (ROC) curve was used to multivariate prediction of the optimal area under curve (AUC) and critical value. Single factor analysis adopted χ2 test, and the influencing factors analysis adopted the multiple Logistic regression. Results? In this study, a total of 827 questionnaire were sent out and 810 of them were valid with 97.94% for the valid recovery rate. There were 289 (35.68%) clinical nurses with lower limb varicose veins. ROC curve analysis showed that the high-risk cutoff values of ages, nursing years and walk time during work of lower limb varicose veins were 29 years old (AUC=0.611, P＜ 0.001, 95%CI:0.572-0.650), 8 years (AUC=0.623, P＜ 0.001, 95%CI:0.584-0.661) and four hours (AUC=0.604, P＜0.005, 95%CI:0.565-0.643) respectively in nurses. Single factor analysis showed that there were statistical differences in the sexes, ages, education levels, positional titles, nursing years, whether having the family history of varicose veins, whether having the history of deep vein thrombosis, night shift conditions, childbearing history, whether taking oral contraceptives, wearing elastic stockings and walk time during working (P＜0.05). Multiple Logistic regression analysis found that the main influencing factors of lower limb varicose veins of clinical nurses included ages, nursing years, having family history of varicose veins, wearing elastic stockings and walk time during working (P＜0.05). Conclusions? The incidence of lower limb varicose veins is high in nurses because of profession with the phenomenon of universally inadequate protective measures. Reasonable and comprehensive protection measures should be taken positively to improve the current situation. Hospital managers should attach importance to the situation, and develop related knowledge training or provide elastic stockings for nurses by taking a perspective of caring staff to reduce the occupational injuries of nurses and extend their careers.

Objective To investigate the effect of stromal cell-derived factor-1α( SDF-1α) and interleukin ( IL-1β) on inducing vascular endothelial cells to express lymphatic phenotype .Methods The CRL-1730 cell line was cultured and treated with SDF-1αor IL-1β.The expression of endothelial cell markers and lymphatic endothelial cell markers were investigated with Real-time PCR, Western blotting and immunocytochemistry .Results In CRL-1730 cell line, endothelial cell markers such as voln willebrand factor ( vWF ) , VE-cadherin , vascular endothelial growth factor receptor(VEGFR)2, were dose dependently down-regulated after SDF-1αstimulation, while lymphatic phenotypes such as Prox-1, podoplanin and lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1), were dose-dependently up-regulated after SDF-1αstimulation.The changes of vWF, VEGFR2 and podoplanin, Prox-1, LYVE-1 expression after IL-1βstimulation was similar to that after SDF-1αwhile expression of VE-cadherin changed slightly .Conclusion SDF-1αand IL-1βare able to induce vascular endothelial cell expressing lymphatic phenotype .

Objective To investigate the effects of Sinopodophyllum hexundrum on apoptosis in K562 cells.Methods K562 cells were treated with Sinopodophyllum hexundrum at different concentrations and for different lengths of time to determine the optimal conditions of SinoPodophyllum hexandrum treatment for K562 cells using CCK8 assay.The cell apoptotic rate was detected by flow cytometry,and the cell morphology and nuclear morphology of K562 cells were observed with Wright staining and DPAI staining,respectively.The protein expressions of BCR/ABL,p-BCR/ABL,STAT5,p-STAT5 and the apoptosis-related proteins PARP,caspase-3 and cleaved-caspase-3 were determined with Western blotting.Results The ceil proliferation was inhibited in a concentration-and time-dependent manner by 1,2,and 3 μg/mL Sinopodophyllum hexundrum.The treatment was optimal with a Sinopodophyllum hexundrum concentration of 2 μg/mL a treatment time of 48 h,and the cell apoptotic rate increased in a time-dependent manner and significantly increased at 48 h (P＜0.001).The expression of apoptosis-related proteins PARP,caspase-3 and cleaved-caspase-3 were also activated in a time-dependent manner.The cells showed typical apoptotic changes after treatment with 2 μg/mL Sinopodophyllum hexundrum for 48 h with significantly reduced expressions of BCR/ABL,p-BCR/ABL,STAT5,AND p-STATS.Conclusion Sinopodophyllum hexundrum promotes K562 cell apoptosis possibly by inhibiting BCR/ABL-STAT5 survival signal pathways and activating the mitochondrion-associated apoptotic pathways.

Objective To investigate the effects of Sinopodophyllum hexundrum on apoptosis in K562 cells.Methods K562 cells were treated with Sinopodophyllum hexundrum at different concentrations and for different lengths of time to determine the optimal conditions of SinoPodophyllum hexandrum treatment for K562 cells using CCK8 assay.The cell apoptotic rate was detected by flow cytometry,and the cell morphology and nuclear morphology of K562 cells were observed with Wright staining and DPAI staining,respectively.The protein expressions of BCR/ABL,p-BCR/ABL,STAT5,p-STAT5 and the apoptosis-related proteins PARP,caspase-3 and cleaved-caspase-3 were determined with Western blotting.Results The ceil proliferation was inhibited in a concentration-and time-dependent manner by 1,2,and 3 μg/mL Sinopodophyllum hexundrum.The treatment was optimal with a Sinopodophyllum hexundrum concentration of 2 μg/mL a treatment time of 48 h,and the cell apoptotic rate increased in a time-dependent manner and significantly increased at 48 h (P＜0.001).The expression of apoptosis-related proteins PARP,caspase-3 and cleaved-caspase-3 were also activated in a time-dependent manner.The cells showed typical apoptotic changes after treatment with 2 μg/mL Sinopodophyllum hexundrum for 48 h with significantly reduced expressions of BCR/ABL,p-BCR/ABL,STAT5,AND p-STATS.Conclusion Sinopodophyllum hexundrum promotes K562 cell apoptosis possibly by inhibiting BCR/ABL-STAT5 survival signal pathways and activating the mitochondrion-associated apoptotic pathways.

Objective:To standardize the management of auditory hallucination symptoms in inpatients with mental illness and develop an expert consensus on the management of auditory hallucinations in hospitalized psychiatric patients.Methods:From March 2023 to July 2023, the Mental Health Committee of the Chinese Nursing Association focused on the key issues in the management of auditory hallucinations symptoms in inpatients with mental illness, based on clinical practice, using literature analysis combined with the work experience of mental health experts, and formed the first draft of the expert consensus on the management of auditory hallucinations in inpatients with mental illness (hereinafter referred to as the consensus). Through 3 rounds of expert consultation and 3 rounds of expert demonstration meeting, the draft was adjusted, revised, and improved.Results:37 experts were included in the Delphi expert consultation, 1 male and 36 females with 39-67(51.48 ± 6.61) years old. The positive coefficients of experts in 3 rounds of Delphi expert consultations were all 100%, and the degrees of expert authority were 0.924, 0.938 and 0.949, respectively. The average importance value of each item was higher than 4.00, the variation coefficient of each item was less than 0.25. The Kendall harmony coefficient of the experts were 0.179, 0.195 and 0.198, respectively (all P<0.05). There were 15, 12, 12 experts in the first, seeond, third rounds of expert demonstration meeting. Finally, a consensus was reached on the recommendation of 4 parts, included auditory hallucination assessment, management format, symptom management implementation, and precautions. Conclusions:The consensus covers all parts of the management of auditory hallucination symptoms in hospitalized patients with mental disorders, which is practical and scientific. It is helpful to guide mental health professionals to standardize the management of auditory hallucination symptoms, improve the quality of nursing and ensure the safety of patients.