[Objective]To summarize the academic experience of Professor ZENG Qingqi in the use of classical prescription in the diagnosis and treatment of insomnia combined with reproductive system diseases.[Methods]Through clinical follow-up studying,reading ancient books,Professor ZENG's clinical experience in treating insomnia and reproductive system diseases was analyzed from the aspects of the treatment of insomnia with classical prescription,the treatment ideas of reproductive system diseases with classical prescription,and the concept of homotherapy for heteropathy in Chinese medicine for insomnia combined with reproductive system diseases,which was supported by medical cases.[Results]Professor ZENG's treatment of insomnia combined with reproductive system diseases follows the concept of homotherapy for heteropathy in Chinese medicine,advocating the use of the"five differentiation"diagnostic and treatment mode based on Chinese medicine viewpoint,such as body differentiation,disease differentiation,syndrome differentiation,local differentiation and micro differentiation,and paying attention to promoting medication according to the situation,combining warming and clearing,dredging and tonifying.It is believed that the key to the effectiveness of classical prescription in treating insomnia combined with reproductive system diseases lies in dealing with the problems of"suppressing hyperactivity"and"releasing",emphasizing the reconciliation between the relationship of heart(brain),heart and kidney,and not only dredging to treat the symptoms,but also cultivating and replenishing to treat the root cause.The patient in case one was of insomnia combined with impotence,identified as liver depression and Qi stagnation,spleen and kidney deficiency syndrome,modified Sini Powder and Shenqi Pills was used in treatment.Case two was of insomnia combined with menstruation,identified as disconnection of the heart and kidney,deficiency cold of Chong Ren and blood stasis blocking the uterus,treated with Huanglian Ejiao Decoction and Zhizishi Decoction combined with Wenjing Decoction.Both used classical prescriptions and achieved good results.[Conclusion]Professor ZENG specializes in the treatment of insomnia combined with reproductive system diseases with the use of meridian prescription,flexible identification,and appropriate prescription,with precise efficacy,its rich clinical experience is worth clinical learning and further promotion.

Objective:To explore the long-term preservation value and repair effect of normothermic machine perfusion (NMP) on clinically discarded kidneys.Methods:A case of clinical discarded donor kidney was collected, and NMP was carried out in vitro for 9 hours with recovered blood. The dynamic changes of renal appearance, blood gas and biochemistry analysis of perfusate and renal pathology were recorded. Results:In the second to fifth hour of NMP, the appearance of renal was pink and ex vivo normothermic perfusion assessment score (EVNP) was grade Ⅰ. While, the sixth hour and beyond of NMP, the appearance of kidney turned to dark red and EVNP was grade Ⅲ. The renal perfusion blood flow maintained above 150 ml/min in the first 6 hours and decreased significantly after that, and at the end, was only 50 ml/min. During the whole process of perfusion, urine output was maintained at about 100 ml/h. PO 2 remained above 100 mmHg in the first 5 hours of perfusion and from the 6th hour, was lower than 80 mmHg and continued to decline, and was close to 0 at the end of perfusion. The results showed that although the K + concentration changes in blood and urine in the first 5 hours of NMP had a good consistency, the lactic acid level had been rising. In addition, there was no significant change in the histopathology at the fourth hour of perfusion compared with that before zero-point puncture, and the fibrinous thrombus in glomeruli was improved compared with that before perfusion. However, at the sixth hour after perfusion and before the end of perfusion, the pathological changes of renal tissue were significantly worse. There were a large of thrombosis in glomerular blood vessels, renal tubular atrophy and acute tubular necrosis. Conclusions:NMP can realize the evaluation of extended criteria donors before transplantation, and it proves the feasibility and repair potential of NMP in kidney to a certain extent. At the same time, NMP also provides a new way to expand the source of donor kidney and to pre-treat organ in vitro.