1.Difference in lymphocyte heat shock protein 71 (HSP71) expression in soldiers of armed police forces from different climatic zones.
Huasong ZENG ; Chengfeng XIAO ; Xiujing JING ; Miao YANG ; Lei KE ; Qian XU ; Hao TAN ; Fangze LI ; Tangchun WU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(4):282-284
OBJECTIVETo analyze the difference between basal and heat-inducible levels of lymphocyte heat shock protein 71 (HSP71) expression in soldiers from Beijing, Zhengzhou and Guangzhou.
METHODSFlow cytometry and Comet assay were used to detect the level of HSP71 and DNA damage respectively.
RESULTSComet assay showed that there was no significant DNA damage before and after heat stress at 41 degrees C for 1 h, and also no difference found among the 3 climatic zones(P > 0.05). HSP71 of all soldiers in the 3 zones elevated after stress (P < 0.05). The basal and heat-inducible levels of HSP71 in Beijing soldiers(845.87 +/- 135.60 and 1254. 47 +/- 239.05 mean fluorescence intensity respectively) were higher than those in Guangzhou soldiers(702.73 +/- 184.70 and 861.72 +/- 225.12 mean fluorescence intensity respectively) (P < 0.05).
CONCLUSIONThe differences of lymphocyte HSP71 expression before and after heat stress among the soldiers from Beijing, Zhengzhou and Guangzhou suggest that basal and heat-inducible levels of lymphocyte HSP71 expression may be considered as a valuable index to evaluate heat tolerance of soldiers in different climatic zones.
Climate ; Comet Assay ; DNA Damage ; Flow Cytometry ; Heat Stress Disorders ; metabolism ; Heat-Shock Proteins ; biosynthesis ; Humans ; Lymphocytes ; metabolism ; Military Personnel ; Police
2.Promotion effect of FOXCUT as a microRNA sponge for miR-24-3p on progression in triple-negative breast cancer through the p38 MAPK signaling pathway.
Xiafei YU ; Fangze QIAN ; Xiaoqiang ZHANG ; Yanhui ZHU ; Gao HE ; Junzhe YANG ; Xian WU ; Yi ZHOU ; Li SHEN ; Xiaoyue SHI ; Hongfei ZHANG ; Xiao'an LIU
Chinese Medical Journal 2024;137(1):105-114
BACKGROUND:
Triple-negative breast cancer (TNBC) is a type of highly invasive breast cancer with a poor prognosis. According to new research, long noncoding RNAs (lncRNAs) play a significant role in the progression of cancer. Although the role of lncRNAs in breast cancer has been well reported, few studies have focused on TNBC. This study aimed to explore the biological function and clinical significance of forkhead box C1 promoter upstream transcript (FOXCUT) in triple-negative breast cancer.
METHODS:
Based on a bioinformatic analysis of the cancer genome atlas (TCGA) database, we detected that the lncRNA FOXCUT was overexpressed in TNBC tissues, which was further validated in an external cohort of tissues from the General Surgery Department of the First Affiliated Hospital of Nanjing Medical University. The functions of FOXCUT in proliferation, migration, and invasion were detected in vitro or in vivo. Luciferase assays and RNA immunoprecipitation (RIP) were performed to reveal that FOXCUT acted as a competitive endogenous RNA (ceRNA) for the microRNA miR-24-3p and consequently inhibited the degradation of p38.
RESULTS:
lncRNA FOXCUT was markedly highly expressed in breast cancer, which was associated with poor prognosis in some cases. Knockdown of FOXCUT significantly inhibited cancer growth and metastasis in vitro or in vivo. Mechanistically, FOXCUT competitively bounded to miR-24-3p to prevent the degradation of p38, which might act as an oncogene in breast cancer.
CONCLUSION
Collectively, this research revealed a novel FOXCUT/miR-24-3p/p38 axis that affected breast cancer progression and suggested that the lncRNA FOXCUT could be a diagnostic marker and therapeutic target for breast cancer.
Humans
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Cell Line, Tumor
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Cell Movement/genetics*
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Cell Proliferation/genetics*
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Gene Expression Regulation, Neoplastic/genetics*
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MAP Kinase Signaling System
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MicroRNAs/metabolism*
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p38 Mitogen-Activated Protein Kinases/metabolism*
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RNA, Long Noncoding/metabolism*
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Triple Negative Breast Neoplasms/pathology*