Objective:To prepare lactosaminated matrine liposomes and to study its targetability to liver in mice and efficacy of anti-tumor activities on human hepatoma cell line HepG2 in vitro.Methods:The matrine liposomes were prepared using the reverse-phase evaporation-ultrasonic technique.Lactosylphosphatidy-lethanolamine(Lac-PE) was synthesized and used for modified matrine liposomes..the diameter and entrapment efficiency of it were determined.The RP-HPLC was used for the determination of matrine concentration in mice tissue.The cytotoxic effect of LML on human hepatoma cell line HepG2 in vitro was detected by thiazolyl blue(MTT)assay.Results:The LML were nice and uniform,the particle diameter was between 80 and 150 nm and envelopment rats was 48.1%.Compared with matrine solution,ML and LML exhibited long circulation time.Tissue distribution results proved that the area under curve of liver was significantly difference among modified matrine liposomes,regular matrine liposomes and matrine solutions(P

Objective:To evaluate the effectiveness of endoscopic minimally invasive treatment for submucosal tumors of the esophagus.Methods:Included in the study were 33 patients with esophageal submucosal tumors,male 23 and female 10 cases,aged 34-85 years(average 51.2).The lesions were removed by means of endoscopic mucosal resection(EMR),or stripping-resection method,i.e.,first stripped by a needle knife and followed by "injection and cut".Results:All the lesions were resected completely by endoscopic minimally invasive methods.Of 33 lesions,15 cases were undertook EMR,and 18 cases were treated by stripping-resection method.Except 5 cases of treatable bleeding,and 6 cases of post-operative substernal pain,no other complications were found.Pathological exam and immunohistochemical study showed that 25 cases were esophageal leiomyoma,3 cases of stromal tumor,2 cases of lipoma,2 cases of haemangioma and 1 case of submucoal cyst.No recurrence was found during the follow-up of 2-30 months. Conclusion:Endoscopic minimally invasive therapy is effective and safe for the treatment of esophageal submucosal tumors.

The diagnosis and monitoring of inflammatory bowel disease mainly depend on clinical presentation , endoscopical , histopathological and imaging findings .It has been demonstrated that fecal inflammatory biomarkers can be potential markers to replace endoscopy diagnosed disease activity .Calprotectin is a binding protein of calcium and zinc , which is mainly derived from neutrophils and macrophages .Calprotectin is an accurate noninvasive biomarker in diagnosing and monitoring inflammatory bowel disease in clin -ics.This paper reviews the progress of fecal calprotectin in the diagnosis, disease activity evaluation, clinical monitoring and prognosis of inflammatory bowel disease .

Mannan binding lectin (MBL) is an innate immune mediator belonging to the collectin family known to bind to the surface of many viruses, bacteria and fungi. Many researches show that MBL is associated with many autoimmune diseases. Inflammatory bowel diseases (IBD) including ulcerative colitis and Crohn's disease are related with innate individual immunity. This article reviews the recent researches on the association of MBL with IBD.

Objective To evaluate the efficacy and safety of Levofloxacin combined triple viable bacteria tablets of Lactobacillus in the treatment of acute diarrhea.Methods65 cases of acute diarrhea patients were randomly divided into control group with 32 cases and experiment group of 33 cases.Patients were given antiemetic, fever and nutritional supplements and other symptomatic treatment,the control group was treated with ofloxacin injection 10mL+5% glucose solution 250mL for an intravenous injection, 3 days as a course of treatment;The experiment group was treated on the basis of triple viable bacteria tablets of Lactobacillus 4 tablets, 3 times a day, 3 days for a course of treatment.Clinical curative effect, adverse reaction rate, serum TNF-α, IL-6, CD4+, CD8+ and CD8+ CD4+/ level were compared.ResultsThe total effective rate in the experimental group was significantly higher than that in control group(P<0.05), there was no significant difference in the incidence of adverse reactions between the two groups.The levels of IL-6 and TNF-α were decreased in 2 groups (P<0.05), the levels of CD4+ in the two groups were decreased (P<0.05), and the CD4+ level was lower in the experiment group (P<0.05);the CD4+/CD8+ level was increased (P<0.05), and the CD4+/CD8+ level was higher in the experiment group (P<0.05).ConclusionThe clinical efficacy of levofloxacin combined with triple viable bacteria tablets of Lactobacillus in the treatment of acute diarrhea is significant, and the safety is high.

Objective To study curative efficacy of Pramipexole in treatment of Parkinson's Disease and its effects on Serum C-reactionprotein (CRP), cystatin C, brain derived neurotrophic factor(BDNF) and oxidative stress.Methods 100 patients of Parkinson's Disease who received therapy from February 2014 to April 2016 in our hospital were selected as research objects were randomLy divided into control group and observation group, 50 patients in each group.The control group was treated with Dobasidate tablets;The observation group was given pramipexole tablets on the basis of the control group.Then the clinical effects and adverse reactions were compared.CRP, Cystatin C, BDNF and oxidative stress were measured. Results After treatment, the total effective rate of the observation group [(90.00% (45/50)] was significantly higher than the control group[74.00%(37/50)] (P<0.05);The symptoms of palpitation, depression, nausea, anorexia, diarrhea and vomiting were lower in observation group than in observation group, and the differences of depression, anorexia and vomiting were statistically significant(P<0.05);The BDNF index of the observation group (13.38 ±1.93)ng/L was higher than that of the control group (12.03 ±1.83) ng/L(P<0.05);CRP (3.53 ±0.97) mg/L and Cystatin C index (0.98 ±0.12) mg/L were lower than the control group(4.62 ±1.15)、(1.06 ±0.14) mg/L (P<0.05).After treatment, the glutathione peroxidate (GPX),superroxide dismutase(SOD),glutathione(GSH)and catalase(CAT) of oxidative stress in observation group were higher than those in control group,the difference was statistically significant (P<0.05).Conclusion Pramipexole in the treatment of Parkinson's disease is obvious, can effectively improve the serum CRP, Cystatin C and BDNF index, high security, it is worth promoting the application.

The biological effects of Songzike acidic polysaccharide (ST90) were studied in vivo and vitro. It was found that ST90 had distinct antineoplastic, antibacterial and antiviral effects.I markedly inhibits proliferation of S180 solid tumor in mice and reduces the mortality of mice infected by Salmonella typhimurium, and shows a protective effect on FL cells infected with adva、adv7, vsv and CB4.

Objective To explore the effects of interactive health education on the knowledge and behaviors of medicine use and the biochemistry indicators in hemodialysis patients. Methods Forty-eight eligible hemodialysis patients were intervened with interactive health education once every month. After three times'intervention, phosphorus, hemoglobin and hematocrit level were compared before and after the intervention. Results After the intervention, phosphorus, levels of hemoglobin and hematocrit were all significantly improved ( P<0 . 001 ) compared with that before the intervention . Conclusion The interactive health education can improve the knowledge and compliance behaviors in medicine use, serum phosphate and symptoms of anemia in hemodialysis patients.

OBJECTIVE:To evaluate the clinical efficacy of cinepazide maleate in combination with magnesium sulfate for traumatic subarachnoid hemorrhage.METHODS:A total of 160 patients with traumatic subarachnoid hemorrhage were randomly assigned to 4 groups:Control group(n=40)received conventional therapy alone(hemostasis,dehydration,hormone and drugs promoting recovery of nervous function;Group Ⅰ(Control group,n=40)received cinepazide maleate plus conventional therapy;Group Ⅱ(n=40)received magnesium sulfate plus conventional therapy,and Group Ⅲ(n=40)received cinepazide maleate in combination with magnesium sulfate in additional to the conventional therapy.All the drugs were given in every 12 h for 14 consecutive days.Transcranial Doppler(TCD)examination was scheduled at 0,3,7,14 days after admission to observe the incidences of cerebral angiospasm(CVS)and patients' prognosis.RESULTS:There were significant differences between Group Ⅲ and the other 3 groups(Control group,Group Ⅰ and Group Ⅱ)in the incidence of CVS,the change of the blood flow rate of the middle cerebral artery and patients' prognosis at 3 and 6 months respectively(P

Objective To analyze the effect of one-part form mini-implant in treatment of immediately and delayed repair of edentulous space. Methods Retrospectively analyzed 80 cases of edentulous space patients in our hospital from Jan. 2009 to Jan. 2011, and divided them into the immediately repair group ( observation group) and the delayed repair group ( control group) according to time of edentulous space repair. Patients of the two groups were given permanent PFM 3 months later, and regular clinical and X-ray examinations were made to evaluated clinical efficacy and complications of the two groups. Results The score of ISQ of the observation group was higher than that in the control group with significant difference (P <0. 05); and the postoperative complications of the observation group was significantly decreased compared to the control group with a significant difference (P <0. 05). Conclusion Mini-implant used in immediately repair in edentulous space can remove pain in a short time with less postoperative complications, it has more advantages than delayed repair of edentulous space.