Objective To evaluate the effects of GLYX-13 on cognitive function after long-time isoflurane anesthesia in mice. Methods A total of 192 healthy male C57∕B6J mice, aged 8 weeks, weig-hing 22-25 g, were divided into 4 groups(n=48 each)using a random number table: control group (group C), isoflurane anesthesia group(group I), GLYX-13 group(group G), and isoflurane anesthesia plus GLYX-13 group(group IG). The animals were exposed to 15% isoflurane for 6 h in I and IG groups. GLYX-13 1 mg∕kg was injected via the caudal vein at 2 h before anesthesia in G and IG groups. Novel ob-ject recognition test and contextual fear conditioning test were performed on 1st, 3rd and 7th days after an-esthesia. The expression of 2B subunits-containing NMDA receptor(NR2B)and cyclic adenosine mono-phosphate response element-binding protein(CREB)mRNA in the hippocampus was detected by quantita-tive real-time polymerase chain reaction after the end of behavioral tests on 1st, 3rd and 7th days after anes-thesia. Results Compared with group C, the percentage of time spent in exploring a novel object, dis-crimination index and percentage of freezing time were significantly decreased, and the expression of NR2B and CREB mRNA in the hippocampus was down-regulated in group I(P <005). Compared with group I, the percentage of time spent in exploring a novel object, discrimination index and percentage of freezing time were significantly increased, and the expression of NR2B and CREB mRNA in the hippocampus was up-regulated in group IG(P <005). Conclusion GLYX-13 can significantly improve the cognitive func-tion after long-time isoflurane anesthesia in mice.

Objective:To explore the effect of diet intervention based on information-motivation-behavioral skill (IMB) model in patients with diabetes combined with gout.Methods:From January 2019 to September 2021, diabetes patients with gout admitted to the Hangzhou First People's Hospital were selected by convenience sampling. According to the random number table method, patients were divided into a control group (60 cases) and a study group (60 cases). Both groups received symptomatic treatment and routine nursing. On this basis, the control group received routine diet intervention, while the research group received diet intervention based on the IMB model. The Diet Compliance Questionnaire and the Chinese version of the World Health Organization Quality of Life Measurement Scale-Brief Version (WHOQOL-BREF) were used to evaluate the diet compliance and quality of life of two groups of patients. This study measured the blood uric acid, glycosylated hemoglobin (HbA1c), and fasting blood glucose (FPG) in two groups of patients to evaluate their health condition.Results:After intervention, the scores of knowledge, attitude and practice dimensions in the Diet Compliance Questionnaire of patients in the study group were higher than those in the control group, and the Chinese version of WHOQOL-BREF score was higher than that in the control group, with statistical differences ( P<0.05). After intervention, the blood uric acid, FPG, and HbA1c in the study group were lower than those in the control group, with statistically significant differences ( P<0.05) . Conclusions:The diet intervention based on IMB model can improve the diet compliance of diabetes patients with gout, reduce the blood glucose and uric acid of patients, and improve the quality of life of patients.