Objective Stromal and glandular cells of human endometrium were successfully isolated and cultured in this experiment. Methods These two types of cells were observed by light microscopy and verified by immunocytochemical staining. Results The result showed that some of stromal cells became adherent after.0 5h of culture.Two shapes of stromal cells were found.One was spindle and the another was polygonal.Immunocytochemical staining method showed that both shapes of stromal cells were positive for vimentin,with 95% of positive rate,and negative for cytokeratin 1,indicating that these cells were endometrial stromal cells.Most of glandular cells became adherent after 24h of culture and formed tightly packed whorls after 4d.Individual cells were polygonal and had a large and round nucleus.Immunocytochemical staining method showed that glandular epithelial cells were positive for cytokeratin 1,with 90% of positive rate,suggesting their nature of epithelial cells. Conclusion Stromal and glandular cells of human endometrium were successfully isolated by using two series of filter and cultured in this experiment. [

Capillary zone electrophoresis (CZE) was used to monitor the whole unfolding process of bovine insulin resulted from the dithiothreitol (DTT) reduction. An off-line matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was employed to determine the molecular weight of the reaction products at the same time to confirm the observation results obtained by CZE. The structural change during the process of bovine insulin unfolding could be observed directly from the electropherogram and information of protein unfolding could be obtained simultaneously. The results indicated that as an effective tool of monitoring the conformational change of protein,the method of CZE was simple,quick,sensitive and lower sample consumption.

Aim To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel. Methods Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9 984 genes and expressed sequence tags (ESTs).Results Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.

OBJECTIVETo assess the value of karyotype analysis and fluorescence in situ hybridization(FISH) assay for the diagnosis of myelodysplastic syndrome (MDS).

METHODSThe karyotypes of 122 initially treated MDS patients were analyzed with conventional R-banding and FISH using probes including GLP CSF1R/D5S23, D5S721, GLP EGR1/D5S23, D5S721, GLP D7S486/CSP7, GLP D7S522/CSP7, GLP D20S108, CSP8 and CSP X/Y.

RESULTSThe detection rate of chromosomal abnormalities was 54.9% for the 122 patients. Among these, those involving 3 or more chromosomes are most common (16.4%), followed by +8(14.8%), -7/7q-(7.4%), -5/5q-(5.7%), 20q-(2.5%), and -Y in male patients (5.0%). Two MDS-RAEB II patients detected with t(8;21) should be diagnosed with acute myelocytic leukemia. FISH analysis showed that 54 patients were positive (44.3%). Among these, 30.3% had CSP8 amplification, followed by GLP D7S486/CSP7 and GLP D7S522/CSP7 deletion (12.3%), GLP CSF1R/D5S23, D5S721 and GLP EGR1/D5S23, D5S721 deletion (9.8%), GLP D20S108 deletion (7.4%), and CSPX/Y deletion (5%).

CONCLUSIONWith a detection rate of 54.9%, R-banding still constitutes the basic examination for MDS. As detection of interstitial chromosomal abnormalities in MDS can be greatly enhanced by FISH, combined karyotype analysis and FISH can improve the diagnosis of MDS and facilitate assessment of its prognosis.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Chromosome Aberrations ; Chromosome Banding ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Karyotyping ; Male ; Middle Aged ; Myelodysplastic Syndromes ; genetics ; Sequence Deletion ; Young Adult

OBJECTIVETo investigate the development of xenografted primitive human germ cells by using fetal testicular tissues as donor tissues and an immunodeficient mouse as the recipient.

METHODSTesticular tissue fragments of a 26-week fetus were grafted under the back skin of a castrated immunodeficient mouse. Grafts were taken out after 135 days and processed for morphological and histological analyses.

RESULTSThe mass of grafts grew from about 1 mm in diameter and 5 mg in wet weight to about 3 mm and more than 20 mg 135 days after grafting. Histological observations showed a significant expansion of seminiferous tubules after grafting (80 +/- 25 microm in diameter) in comparison with seminiferous cords at the time of grafting (60 +/- 15 microm in diameter). The seminiferous cords developed into seminiferous tubules with the epithelial border and lumen. After 135 days of grafting, most of the dispersedly distributed primitive Sertoli cells and germ cells migrated to the basal part of seminiferous epithelium, located on the basement membrane and few of germ cells differentiated into spermatogonia.

CONCLUSIONHuman fetal testicular tissues could survive and continuously develop after being xenograft into castrated immunodeficient mice.

Animals ; Fetal Tissue Transplantation ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Spermatids ; growth & development ; Testis ; cytology ; transplantation ; Transplantation, Heterologous

Objective To investigate the impact of salpingectomy with preservation of fallopian tube mesentery on ovarian reserve function.Methods A total of 281 patients with tubal disease who were admitted from January 2020 to March 2021 were collected as research objects.According to the different treatment methods,they were divided into traditional bilateral resection group(n=53,con-ventional bilateral salpingectomy),traditional unilateral resection group(n=56,conventional unilat-eral salpingectomy),preservation of bilateral resection group(n=60,bilateral salpingectomy with preservation of fallopian tube mesentery),preservation of unilateral resection group(n=54,unilater-al salpingectomy with preservation of fallopian tube mesentery),and control group[n=58,patients with in vitro fertilization-embryo transfer(IVF-ET)but no history of salpingectomy].The operation time,intraoperative blood loss,and postoperative exhaust time were compared among the groups.Two months after the operation,IVF-ET was performed,and the number of follicles and oocytes,pregnan-cy rate,and fertilization rate were detected.The levels of anti-mullerian hormone(AMH),follicle-stimulating hormone(FSH),estradiol(E2),and luteinizing hormone(LH)in peripheral blood were detected after IVF-ET.Follow-up was conducted until March 2023,and pregnancy success rates were recorded for the five groups.Results There were no significant differences in perioperative opera-tion time,intraoperative blood loss,and postoperative exhaust time among traditional bilateral resec-tion group,traditional unilateral resection group,preservation of bilateral resection group,preserva-tion of unilateral resection group(P＞0.05).After IVF-ET,there were significant differences in AMH,FSH,E2,and LH levels among the five groups(P＜0.05).The levels of AMH and E2 in a rising sequence were traditional bilateral resection group,traditional unilateral resection group,pres-ervation of bilateral resection group,preservation of unilateral resection group and control group,while the levels of FSH and LH in descending sequencing were traditional bilateral resection group,traditional unilateral resection group,preservation of bilateral resection group,preservation of unilat-eral resection group and control group.Follow-up was conducted until March 2023,and the preg-nancy success rates after IVF-ET were 45.28％,50.00％,53.33％,59.26％,and 58.62％in the traditional bilateral resection group,traditional unilateral resection group,preservation of bilater-al resection group,preservation of unilateral resection group,and control group,respectively.There was no significant difference in pregnancy success rate after IVF-ET among the five groups(x2=3.044,P=0.551).Conclusion Compared with traditional salpingectomy,salpingectomy with preservation of fallopian tube mesentery has less impact on ovarian reserve function.

Objective To investigate the impact of salpingectomy with preservation of fallopian tube mesentery on ovarian reserve function.Methods A total of 281 patients with tubal disease who were admitted from January 2020 to March 2021 were collected as research objects.According to the different treatment methods,they were divided into traditional bilateral resection group(n=53,con-ventional bilateral salpingectomy),traditional unilateral resection group(n=56,conventional unilat-eral salpingectomy),preservation of bilateral resection group(n=60,bilateral salpingectomy with preservation of fallopian tube mesentery),preservation of unilateral resection group(n=54,unilater-al salpingectomy with preservation of fallopian tube mesentery),and control group[n=58,patients with in vitro fertilization-embryo transfer(IVF-ET)but no history of salpingectomy].The operation time,intraoperative blood loss,and postoperative exhaust time were compared among the groups.Two months after the operation,IVF-ET was performed,and the number of follicles and oocytes,pregnan-cy rate,and fertilization rate were detected.The levels of anti-mullerian hormone(AMH),follicle-stimulating hormone(FSH),estradiol(E2),and luteinizing hormone(LH)in peripheral blood were detected after IVF-ET.Follow-up was conducted until March 2023,and pregnancy success rates were recorded for the five groups.Results There were no significant differences in perioperative opera-tion time,intraoperative blood loss,and postoperative exhaust time among traditional bilateral resec-tion group,traditional unilateral resection group,preservation of bilateral resection group,preserva-tion of unilateral resection group(P＞0.05).After IVF-ET,there were significant differences in AMH,FSH,E2,and LH levels among the five groups(P＜0.05).The levels of AMH and E2 in a rising sequence were traditional bilateral resection group,traditional unilateral resection group,pres-ervation of bilateral resection group,preservation of unilateral resection group and control group,while the levels of FSH and LH in descending sequencing were traditional bilateral resection group,traditional unilateral resection group,preservation of bilateral resection group,preservation of unilat-eral resection group and control group.Follow-up was conducted until March 2023,and the preg-nancy success rates after IVF-ET were 45.28％,50.00％,53.33％,59.26％,and 58.62％in the traditional bilateral resection group,traditional unilateral resection group,preservation of bilater-al resection group,preservation of unilateral resection group,and control group,respectively.There was no significant difference in pregnancy success rate after IVF-ET among the five groups(x2=3.044,P=0.551).Conclusion Compared with traditional salpingectomy,salpingectomy with preservation of fallopian tube mesentery has less impact on ovarian reserve function.

BACKGROUND:In recent years,the development of liver organoids has made it a hot spot in the field of international liver disease research,but there is still no article on the bibliometric analysis of liver organoids. OBJECTIVE:To explore the hot trends in liver organoids in the last 20 years based on bibliometrics and visualization analysis. METHODS:We searched the articles about liver organoids in the Web of Science Core Collection from January 1,2002 to November 12,2022.Origin,Office,and CiteSpace software were used for bibliometrics and visualization analysis.We statistically analyzed the number of annually published articles,countries,institutions,authors,journals,and keywords of the articles by generating charts. RESULTS AND CONCLUSION:The number of articles,citation frequency,institutions and personnel involved in the research about liver organoids showed an overall upward trend in the last 20 years,indicating that the field was growing rapidly and attention was increasing.The USA had published the most papers and had the strongest influence in this field.Although it had invested a lot of time and energy,the number of papers published by a single research institution in the USA was not the highest among many research institutions.China was second only to the USA in the number of publications,with the Chinese Academy of Sciences and Fudan University leading the list.Utrecht University in the Netherlands was the institution with the most publications.Clevers H was the author with the highest number of articles.The article with the highest co-citation frequency was"Long-term culture of genome-stable bipotent stem cells from adult human liver".The main fields of study for liver organoids were Molecular Science,Biology,and Immunology.The most frequently occurring keywords were stem cell,in vitro,and culture.The research hotspots in the liver organoids field were mainly focused on in vitro stem cell three-dimensional culture,differentiation and gene expression.

OBJECTIVE To investigate the effects of Forsythia suspensa ethanol extract on the proliferation， migration and invasion of lung cancer cells NCI-H226. METHODS As research objects， lung cancer cells NCI-H226 were divided into control group， F. suspensa ethanol extract low-， medium- and high-concentration groups （5， 10， 20 mg/mL）， activator group [10 mg/mL F. suspensa ethanol extract+0.5 μmol/L nuclear factor kappa B （NF-κB） signaling pathway activator PMA]， inhibitor group （10 mg/mL F. suspensa ethanol extract+10 μmol/L NF-κB signaling pathway inhibitor BAY 11-7082） and positive control group （20 μg/mL cisplatin）. Except for the control group of cells without intervention， all other groups of cells were cultured with corresponding drugs for 24 hours； the proliferation， migration and invasion of cells were all detected， and the proliferation rate， migration rate， and the number of invading cells were also calculated； protein expressions of NF-κB p65， NF-κB inhibitory protein α （IκBα）， phosphorylated NF-κB p65 （p-NF-κB p65） and phosphorylated IκBα （p-IκBα） were determined. RESULTS Compared with control group， the proliferation rate， migration rate， and the number of invading cells as well as the protein expressions of p- IκBα and p-NF-κB p65 were decreased significantly in F. suspensa ethanol extract groups and positive control group （P＜0.05）. Compared with F. suspensa ethanol extract medium-concentration group， the proliferation rate， migration rate， and the number of invading cells as well as above protein expressions were all decreased significantly in inhibitor group （P＜0.05）， while those of activator group were increased significantly （P＜0.05）. CONCLUSIONS F. suspensa ethanol extract can inhibit the proliferation， migration and invasion of lung cancer cells NCI-H226， and the mechanism of which may be related to the inhibition of NF-κB signaling pathway.