Objective To observe the effect of Sitagliptin Phosphate Tablets combined with shenmai on type 2 diabetes.Methods Sixty diabetes patients were divided into two groups randomly:treatment group were received Sitagliptin Phosphate Tablets and shenmai;the control group were given acarbose.14 weeks later,the indications FBG,2HBG,HbA1 C,TC,TG,LDL-C,HDL-C,hypoglycemia happen rate,BMI,blood rheology,transcranial Doppler ultrasound(TCD)were tested to see if there is any change.Results After treatment,two groups of FBG,2hBG,HbA1 C,TC,TG,LDL-C,HDL-C levels compared with those before treatment were statistically significant(P＜0.05 or P＜0.01),but the treatment group on lipid control more than those in the control group;treatment group therapy in reducing the risk of hypoglycaemia and weight gain than the control group(P＜0.05);the whole blood viscosity,plasma viscosity,low viscosity and hematocrit of treatment group were significantly lower(P＜0.05),while the control group showed no significant change;the abnormal rate of treatment group was 52.6％,significantly better than the control group 86.5％(P＜0.05).Conclusion The effect of Sitagliptin Phosphate Tablets combined with shenmai on type 2 diabetes was significant,compared with the traditional antidiabetic drugs,the combination is effective hypoglycemic control fat and less adverse reactions.

Objective To explore the relationship between platelet activating factors and four platelet parameters in diabetic retinopathy (DR).Methods Eighty patients in our hospital were chosen from January 2015 to December 2015,regular ophthalmological examination and fundus fluorescence were performed on all cases.On the basis of international clinical diabetic retinopathy classification standard,the patients were divided into four groups:No DR group with 20 cases,non-proliferative diabetic retinopathy (NPDR) group with 20 cases,and proliferative diabetic retinopathy (PDR) group with 20 cases.20 healthy people who received physical examination during the same period were recruited as the control group.The flow cytometry instrument was used to determinate platelet activating factor CD62p and CD63,automatic blood analyzer was used to determinate platelet parameter which including blood platelet count (PLT),mean platelet volume (MPV),plateletcrit (PCT) and platelet distribution width (PDW),and the changes of platelet activation factor,parameters and its clinical significance in DR were discussed.Results With the development of DR,CD62p,CD63,MVP were numerical gradually increased,and PLT were numerical gradually reduced,the differences were statistically significant (all P ＜ 0.05);Compared with control group,CD62p,CD63,MVP were increased and PLT were reduced in other group,the differences were statistically significant (all P ＜ 0.05);Compared with pure diabetes group,CD62p,CD63,MVP were increased and PLT were reduced in NPDR group and PDR group,the differences were statistically significant (all P ＜ 0.05);Compared with NPDR group,CD62p,CD63,MVP were increased and PLT were reduced in PDR group,the differences were statistically significant(all P ＜ 0.05).PDW were numerical gradually increased in control group,pure diabetes group,NPDR group,PDR group,the differences were statistically significant(all P ＜ 0.05);Control group compared with pure diabetes group,NPDR group compared with PDR group,the differences in PDW was not statistical significant (all P ＞ 0.05);But compared with control group or pure diabetes group,PDW of NPDR group and PDR group increased,the differences were statistically significant(all P ＜ 0.05).The differences in PCT among groups was not statistically significant(all P ＞ 0.05).Conclusion The platelet activating factor CD62p,CD63 and parameters of platelet show differences in each diabetic retinopathy period,platelet activating factor in the occurrence and development of diabetic retinopathy have important significance.Monitor the platelet activating factor and parameters of platelet can help surveillance of the disease.

The nanoparticle-based electrochemical technology for early detection of cancer is an imjportant research topic in the area of biomedicine.This article introduces the concept of tumor marker and principle of electrochemical detection of the tumor marker.The applications of nanoparticles in electrochemical early detection of cancer are reviewed in detail.Finally,the prospected application of research is discussed.

Objective To study the levels of interleukin-6(IL-6)and tumor necrosis factor-alfa(TNF- α)in serum and peritoneal fluid of women with endometriosis,to explore their roles in pathogenesis of endometriosis. Methods IL-6 and TNF-α levels in peritoneal fluid and serum of 60 patients with endometriosis(34 cases in Ⅰ ～ Ⅱ stage,26 cases in Ⅲ ～ Ⅳ stage)and 30 control cases(not EMS patients)were detected by enzyme linked immunosorbent assay(ELISA). Results Peritoneal fluid and serum levels of IL-6 and TNF-α in EMS group were significantly higher than that in control group(P＜0.01),and peritoneal fluid and serum levels of IL-6 and TNF-α in Ⅲ ～Ⅳ stage was siguificantly higher than that in Ⅰ ～ Ⅱ stage(P＜0.01). Conclusion IL-6 and TNF-α in peritoneal fluid and serum played an important role in pathogenesis of endometriosis.

Objective To study the laparoscopic uterine artery occlusion effect of assisted myomectomy for ovarian function.Methods 90 cases of existing operation indications ofuterine leiomyoma patients were selected as the research objects and divided into 45 cases of the control group and 45 cases of the study group.The control group received laparoscopic uterine myomectomy,the study group received laparoscopic uterine artery occlusion assisted laparoscopic myomectomy comparison between the two groups,the curative effects of the patients with operation and influence on ovarian function and sexual function.Results In the control group,the operation time (72.03 ± 13.53) min was longer than that of the study group(60.05 ± 10.32) min(t =4.722,P ＜0.05);control group,amount of bleeding during the operation was (125.43 ± 17.69) mL,more than that of the study group (82.46 ± 13.89) mL (t =12.816,P ＜ 0.05);the two group of exhaust time after operation (t =0.711,P ＞ 0.05) the time of hospitalization and postoperative (t =0.466,P ＞ 0.05) showed no significant differences.The control group after 3 months of follicle stimulating hormone (FSH),estradiol (E2) were (6.08 ± 1.35) U/L,(80.31 ± 5.36) pmol/L,the study group after 3 months of FSH,E2 were (6.85 ± 1.42) U/L,(72.39 ± 5.35) pmol/L,the difference between the two groups was statistically significant(t =2.636,t =7.015,P ＜0.05);sexual function in patients of the two groups after scores were significantly higher than those before operation,there were no statistically significant differences between the two groups (P ＞ 0.05).Conclusion Laparoscopic uterine artery blocking effects of laparoscopic assisted myomectomy in patients with ovarian function can recover quickly,safely and reliably.

Aim To study the influence of rosuvastatin on toll-like receptor 4 ( TLR4 ) , and its downstream nu-clear transcription factor NF-kappa B p65, IκBα, in-flammation factors TNF alpha in rats with myocardial hypertrophy induced by pressure overload .Methods Myocardial hypertrophy was induced by abdominal aor-tic constriction ( AAC ) .Male Wistar rats were divided into 3 groups(n=10):① sham-operated rats(S);②AAC rats(M);③AAC+rosuvastatin(10 mg· kg -1· d-1 ) rats.From 1 week pre-opertion to 4 weeks post-operation, three groups were performed by gavage ad-ministration with equal volume of rosuvastatin and vehi-cle.The rats underwent cardiac color Doppler exami-nation.The level of ANP,TLR4, NF-κB p65,IκBα, TNF-αmRNA and protein expression in myocardium were detected by real-time PCR, Western blot, immu-nohistochemical and ELISA respectively .Results The sizes of heart and cardiomyocytes and the expression of ANP mRNA and TLR4 signaling molecules were signif-icantly increased in group M , which could be blocked by rosuvastatin .TLR4 protein was positively related to ANP, NF-κB p65 and TNF-αrespectively .Conclusion Rosuvastatin prevents cardiac hypertrophy induced by pressure overload , which is associated with its inhi-bition of TLR4, NF-κB and TNF-αin myocardium ex-pression .

Gold nanoparticles ( AuNPs) have been widely used in biomedicine due to their unique physical and chemical properties as well as good biocompatibility. Current research in this field has been focused on AuNP radiosensitization in radiotherapy for cancer. Extensive studies in vivo and in vitro have showed the radiosensitization effect of AuNPs. However, the mechanism of radiosensitization by AuNPs still requires further studies. Right now, the radiation?insensitive phase ( G0+G1 phase) to radiation?sensitive phase ( G2+M phase ) transition of tumor cells by AuNPs is widely considered as the main cause of radiosensitization. There are many influencing factors for AuNP radiosensitization such as particle size, surface modification, microscopic distribution, radiation energy, radiation dose, and type of tumor cells. Moreover, safety should also be taken into account in AuNP radiosensitization. Clinical trials of AuNPs have been carried out right now. More studies on AuNP radiosensitization are needed to achieve real clinical transformation.

Objective To evaluate the effect of small-dose Lorazepam on residual dizziness in elderly patients with benign paroxysmal positional vertigo (BPPV) after successful particle repositioning maneuver (PRM).Methods A total of 268 patients aged 60 years and over, who were diagnosed as BPPV and underwent successful treatment of PRM, were randomly assigned to medication group and control group.The patients in the medication group were prescribed low-dose lorazepam for 1 week (0.25 mg/time, 3 times/d), whereas the patients in the control group were not prescribed any medication.Hamilton Anxiety Scale (HAMA) was employed to evaluate the anxiety status of patients before and after PRM, and the effect of small-dose lorazepam on residual dizziness was accessed by using the Dizziness Handicap Inventory (DHI) scale and the Activities-specific Balance Confidence (ABC) scale in elderly BPPV patients after PRM.Results No difference in HAMA scores was found between the two groups (t=-0.316, P=0.753) before PRM.The medication group (t=19.931, P=0.000) and the control group (t=26.930, P=0.000) showed a significant improvement in HAMA scores after PRM versus before PRM.However, HAMA scores after PRM was lower in the medication group than in the control group (t=14.967, P=0.000).The medication group had significant improvements after PRM versus before PRM in the following: DHI scores (t=43.661, P=0.000), functional (t=32.326, P=0.000981), emotional (t=31.981, P=0.000), physical (t=14.330, P=0.000) subscale scores, as well as in the ABC scores (t=-23.248, P=0.000).The improvements were also found in the control group in DHI scores (t=46.282, P=0.000), functional (t=32.117, P=0.000), emotional (t=34.563, P=0.000),physical (t=13.182, P=0.000) subscale scores, as well as in the ABC scores(t=-24.536, P=0.000)after PRM versus before PRM.However, after PRM the total DHI score, functional,emotional and physical subscale scores were lower in medication group than in control group (t=5.994, 3.206, 4.757 and 2.851, respectively, P=0.009, 0.002, 0.000 and 0.005).The ABC scores were higher in medication group than in control group (t=2.678, P=0.008) after PRM.Conclusions The elderly patients with BPPV are often accompanied by symptoms of anxiety.The small-dose Lorazepam can alleviate residual dizziness in elderly BPPV patients after successful PRM.

Objective:To compare quantitatively the quality of the canal isthmus retropreparation among three kinds of ultrasonic ret-rotips.Methods:1 20 maxillary bicuspid teeth with two canals and an isthmus were randomly assigned to 3 groups(n=40).The canal isthmus retropreparation were made respectively using ultrasonic niti tip RE2,diamond tip Berutti and #25K file.Image processing and analysis system were used to measure the preoperative and postoperative parameters for further evaluation.Results:The cutting volume of ultrasonic niti tip RE2 group was significantly less than those of other two groups(P<0.05).Conclusion:Ultrasonic niti tip RE2 is feasible in the canal isthmus retropreparation.

Our department treated one case of neonatal congenital cyst of tongue in March 23, 2015. The clinical manifestation of the case is mainly laryngeal stridor after birth 10d, and when infants were associated with progressive dysphagia. Electrolaryngoscope examination showed the goitre look like the cyst of tongue. Laryngeal CT scanning showed tongue lesions, consider the possibility of large cyst. Bilateral thyroid gland showed good, and no obvious abnormality was found in the cervical ultrasonography. The children was transferred to the ear-nose-throat department for excision of cyst of tongue by low-temperature plasma knife, and postoperative pathology confirmed the diagnosis of cyst of tongue.
Cold Temperature ; Cryosurgery ; Cysts ; congenital ; surgery ; Humans ; Infant, Newborn ; Plasma Gases ; Tomography, X-Ray Computed ; Tongue ; pathology ; surgery