AIM: To evaluate the protective effect of luteolin on endothelial dysfunction induced by tert-butyl hydro-peroxide (t-BOOH). METHODS: We observed the effect of luteolin on t-BOOH-induced contractions in the aorta rings with or without endot helium, which were incubated with luteolin (10-6 to 10-4 mol/L) for 30 min before determining the concentration-response to t- BOOH. Cultured endothelial cell line (ECV304) was pretreated with different concentrations of luteolin (10-6 to 10-4 mol/L) for 30 min and then exposed to 10 -5 mol/L t-BOOH for 24 hours. Cell morphology was observed , and cell viability was determined by MTT assay. Meanwhile, RT-PCR was used to measure the expression of eNOS and COX-1. RESULTS: Increasing concentrations of t-BOOH produced concentration-dependent cont ractions in aorta rings isolated from rats, luteolin effectively attenuated the contraction in a concentration-dependent manner, and the relaxation response was greater in intact endothelium segments. In MTT and RT-PCR assays, luteolin effectively reduced the cytotoxicity of t-BOOH to endothelium cell and increased the expression of nitric oxide synthase (eNOS) mRNA, which was greatly down-regulated by t-BOOH. CONCLUSION: Luteolin effectively protects the endothelium from the impairment of oxidative stress, and the protection could be related to its negative modulation towards t-BOOH-induced contractions in the aorta.

Objective To explore the ability of ABCD2 score + Low density lipoprotein (LDL)(ABCD2L) in predicting early stroke risk after transient ischemic attack (TIA) . Method A total of 165TIA patients were evaluated and classified according to ABCD2 score and ABCD2L score. The occurrences of cerebral infarction were observed at 2th day or 7th day. ROC curve was used to compare the predictive vaule of two scores. Furthermore, according to these two scores, these patients were classified into three risk groups (low, moderate and high ), observed their stroke rate , and compared the differences of three groups with x2 test. Results The two-day risk of stroke was 13.33% and the seven-day risk of stroke was 20. 0% in 165 patients. When the occurrences of cerebral infarction were observed in two days, the area under the curves (AUC) of ABCD2 and ABCD2L was 0. 76 and 0. 80; observed in seven days, the AUC of two scores was 0. 73 and 0. 79. According to the risk stratification of ABCD2 score, in three risk groups,the two-day risk of stroke was 1.9%, 14. 9% and 30. 8%; the seven-day risk of stroke was 3. 8%, 21.8%and 46. 2% ( P ＜ 0. 05 ) . According to the risk stratification of ABCD2 L score, the two-day risk of stroke was 0%, 7. 8% and 31.1% ; the seven-day risk of stroke was 0%, 12. 6% and 44. 4% ( P ＜ 0. 05 ).Conclusions The predictive accuracy of the ABCD2L score is significantly higher than that of ABCD2 score. Furthermore, individuals at high early risk of stroke after TIA can be identified according to the risk stratification of ABCD2L score.

The Internet -based multiple physiological parameters tele -monitoring system is composed of an information collector for multiple physiological parameters, a personal computer and a central server. A P2P structure mixed with a C/S structure is adopted in the software design. To make tele-monitoring and tele-diagnosis available, the system is applied to real-time measuring, analyzing, monitoring and tele-transmitting of multiple physiological parameters. The system is suitable to be used in community hospital and family.

Objective To investigate and evaluate the diagnostic value of transcranial doppler ultrasound (TCD) on brain death in patients with severe craniocerebral injury.Methods Forty-two cases of severe craniocerebral injury from Affiliated Hospital of Jining Medical University were divided into brain death group and survival group,according to the clinical prognosis.TCD was conducted to examine brain blood flow numerical and changed characteristics of the spectrum.Average blood flow velocity (Vm),pulse index (PI),diastolic blood flow in reverse (reverberating flow) and small systolic peaks in early systole (wave of spikes)were documented.TCD parameters were compared between the two groups with t test.Results In the brain death group,frequency of reverberating flow was 86.67％ and frequency of wave of spikes was 13.33％.In survival group,frequency of reverberating flow was 14.81％ and no wave of spikes was recorded.The difference in frequencies of reverberating flow and wave of spikes between the two groups was significant(x2 =28.25,P ＜0.01).The average speed of bilateral middle cerebral artery blood flow in brain death group((20.02±13.96) cm/s) was significantly reduced compared with survival group((56.81± 16.84) cm/s,t=2.30,P＜0.05).Pulse index (PI) values in brain death group (4.02±3.49)were significantly increased compared with survival group (1.24±1.03) (t=2.10,P＜0.05).Conclusion Reverberating flow or wave of spikes is an important marker for brain death in patients with severe craniocerebral injury.TCD may be applied to clinically confirm the diagnosis of brain death.

Objective To compare the postoperative analgesic effect of the single dose of oxycodone and dezocine in patients who underwent gynecological laparoscopic operation. Methods Sixty patients who underwent elective gynecological laparoscopic operation were randomly divided into two groups (n=30): oxycodone group (group O) and dezocine group (group D). Fifteen minutes before the end of surgery, oxycodone 0.1 mg/kg was given in O group, and dezocine 5 mg was given to D group. Twenty minutes before the end of surgery, tropisetron 5 mg was given to both groups. Analgesia was maintained by propofol-remifentanil with TCI. The mean arterial pressure (MAP) and heart rate (HR) of T1, T2, T3 and T4 were recorded respectively in both two groups. After the operation, pain of visual analogue scale (VAS) was assessed in 2 h ,4 h , 6 h and 24 h, respectively. Results There were no significant differences in MAP and HR between two groups at T1, T2, T3 and T4 (P＞0.05). The VAS score was significantly lower in group O than that of group D (P＜0.05). There was significant difference in the incidence of nausea between the two groups (P＜0.05). Conclusion Single dose of oxycodone 0.1 mg/kg can be used for postoperative analgesia after gynecological laparoscopic operation, and which has better analgesia than that of dezocine, except for the adverse reaction of nausea.

To study xanthones from the barks of Garcinia xanthochymus, the constituents were isolated by normal-phase and reverse-phase silica gel column chromatography from the EtOAc extract. Their structures were elucidated by spectral analysis. Three new xanthones were purified and identified as 1,2,5-trihydroxy-6-methoxyxanthone (1), 1,4,6-trihydroxy-5-methoxyxanthone (2), 1,2,7-trihydroxy-4-( 1,1-dimethylallyl) xanthone (3).

Objective To compare the short term prognostic values between the four scores including ABCD,ABCD2,ABCD+ low density lipoprotein (ABCD+ LDL),ABCD2 + LDL after transient ischemic attack (TIA).Methods 235 TIA patients were evaluated according to ABCD score,ABCD2 score,ABCD + LDL score and ABCD2 + LDL score.The occurrence of cerebral infarction was observed at day 2 and 7.The predictive value was compared between the four scores by using receiver operating characteristic (ROC) curve.Patients were classified into 3 risk groups:lowrisk group,moderate-risk group and high-risk group according to ABCD2 L score,and the stroke incidence was compared between the 3 groups by using x2 test.Results The area under ROC curves (AUCs) of ABCD、ABCD2、ABCDL and ABCD2L was 0.68 (95％CI:0.59-0.76),0.71 (95％CI:0.62-0.80),0.73 (95％CI:0.65-0.82) and 0.77 (95％CI:0.69-0.84) in predicting the risk of cerebral infarction at day 2,and were 0.73 (95％CI:0.66-0.81),0.77 (95％CI:0.70 0.84),0.80(95％CI:0.74-0.87) and 0.83 (95％CI:0.76-0.89) in predicting the risk of cerebral infarction at day 7 respectively.Patients were divided into 3 groups:low-risk group (score 0-2) (n =28),moderate-risk group (score 3-5) (n=143) and high-risk group (score 6-8) (n=45) according to ABCD2L score.There were significant differences in stroke incidence at day 2 and 7 between the 3groups (0％,9.1％ vs.32.8％,0％,11.9％ vs.56.3％,x2=26.15,58.87,both P＜0.05).Conclusions ABCD2 score is better than ABCD score in predicting the short-term prognosis of transient ischemic attack.Combining ABCD2 score with LDL can significantly increase the predictive value of ABCD2 score.

Objective To assess left atrial reservoir function based on left atrial filling volume,the left atrial expansion index and left atrial ejection fraction (LAEF) were measured in patients with left ventricular non-compaction cardiomyopathy (LVNC),using real-time three-dimensional echocardiography (RT3DE),to determine the value for LVNC diagnosis.Methods The study included 20 patients diagnosed with LVNC,including 3 patients,aged 4-16 years;8 patients,aged 17-35 years;9 patients,aged 36-55 years;and 20 healthy age-matched control subjects.All patients underwent two-dimensional echocardiography and RT3DE.Results Two-dimensional echocardiography showed no differences between groups (P ＞ 0.05).RT3DE analysis showed that the average left atrial filling volume/BSA,left atrial expansion index,and LAEF were significantly higher in the LVNC group.Conclusion LVNC is associated with increased left atrial reservoir function.RT3DE shows promise for the diagnosis of LVNC.

Silver nanoparticles ( AgNP) , the metallic silver par-ticles with the diameter of 1 ~100 nm are now widely used in many fields. Many researches show that the smaller size of Ag-NP, the stronger toxicity it shows. Generally speaking, AgNP with 20 nm shows strongest toxicity. After entering the body, they are distributed in different organs in the body, and the dis-tribution in the kidney shows a certain gender difference. They also produce some toxic effects after entering body organs. AgNP often exhibit dose effect on the toxicity in vitro cells,while in vivo experiments, their toxic effects change with the different objects and ways of acting. In addition, AgNP can produce toxic effects on reproduction, and may cause parental reproductive activity to deteriorate, and pass the toxic effects to offspring through the placenta to exert a negative influence on the growth and develop-ment of the offspring. The toxicity mechanisms of AgNP are oxi-dative stress injury caused by producing free radicals;metabolic disorders caused by reducing of drug metabolic enzyme activity;and also related gene expression defects and certain molecules, such as transcription factor NF-E2-related factor 2(Nrf2) prote-ase caused by abnormal expression. In short, AgNP can be toxic to organisms, and we must evaluate their biological safety when we use it, to minimize or even avoid the danger it brings about.