Objective To investigate the effects of systematically nursing intervention on performing upper gastrointestinal longitudinal endoscopic ultrasonography. Methods From July 2006 to August 2008, 80 patients with upper gastrointestinal disease were divided into the experimental group and the control group, with 40 patients in each group. The experimental group received mental nursing before endoscopy examination, to comfort and encourage them to eliminate the psychological tension, instructing patients to relax, turning off lights to reduce the horror resulted from seeing insertion of large visual endoscopic ultrasonography into their mouths. While the control group received standard care of gastrointestinal endoscopic examination. The horror, nausea, abrasion of laryngopharynx and repeated operation were compared in the two groups using x2 test. Results The incidence of abnormal conditions in the experimental group was lower than those of the control group. Conclusions Nursing intervention can elevate the success rate of exami-nation, reduce the suffering of patients and ensure the smooth conduct of examination.

Tumor is a reciprocity result of various genes and factors,and its incidence and mortality remain high.Such traditional strategies as surgery,radiotherapy and chemotherapy cannot completely restrain and reverse the growth of tumor cells.So,new and more effective therapeutic methods are being expected.This article updates the development of RNA interfering(RANi) technique in the targeting therapy of the tumor gene.

Hemorrhagic transformation is a common complication after ischemic stroke, especially after thrombolytic therapy. Its disability and mortality are very high. Therefore, it limits the clinical application of thrombolytic therapy. This article reviews the types of hemorrhagic transformation, possible mechanisms and predictors after thrombolysis for ischemic stroke in order to provide reference for clinical treatment and research of ischemic stroke.

Guinea pig as a commonly used laboratory animal is widely used in various fields of biomedical research.The stability of genetic quality directly affects its development and application .Genetic testing is designed to confirm the genetic characteristics of each strain , to verify whether there are genetic mutations and other genetic contamination, to ensure that the test object meets the requirements of this strain .Along with the emerge of biochemical and molecular marker technology , a more convenient and reliable means is provided for research of genetic homozygosity , genetic type detection and genetic quality monitoring of guinea pigs .In this paper, the application and research progress of biochemical, cytological and molecular markers in studies of guinea pig diversity will be summarized , and provide some help for genetic testing guinea pig.

Abstract: Malaria remains a significant public health issue on a global scale. The disease exhibits pronounced sexual dimorphism, with male-infected individuals often presenting more severe symptoms and higher mortality rates compared to females. However, gender factors are frequently overlooked in many experimental studies related to Plasmodium infection. As a primary female sex hormone, estrogen plays a crucial role in regulating immune responses and inflammatory signals, contributing significantly to the gender differences observed in malaria infections. Here, starting from the basic physiological functions and immune regulatory roles of estrogen, review and summarize the epidemiological evidence of gender differences in malaria infection reported in the past, and delve into how estrogen affects malaria infection and its potential roles within the context of the disease. Given the current absence of a comprehensive review on sex differences in malaria in China, we hope that this article will draw researchers' attention to the sexual dimorphism in malaria. Understanding and emphasizing the sexual dimorphism in malaria, as well as the underlying causes, can help us gain a more profound comprehension of the pathology of malaria and the individual immune responses it elicits. This knowledge may provide new insights for related basic research and potentially aid in future malaria prevention and control efforts.

Biomedical Research ; trends ; Breast Neoplasms ; metabolism ; Female ; Humans ; Mucins ; physiology ; Neoplasm Proteins ; physiology

After the development of domestic embedded subject service and its domestic and foreign main models were described, following suggestions were put forward: thinking approach teaching contents and multiple elementary assessment system should be designed based on OBE theory with medical postgraduate general practical medical literature novelty assessment course as its starting point, excellent students should be trained as scientific information detectors by improving their information literacy and practical ability through small scale blended teaching, the professional bottleneck of librarians and talent team construction should be solved by cultivating subject service team for different subjects and strengthening the contacts between different subjects.

Objective To investigate the expression of BRCA1 in chemosensitive and chemoresistant ovarian cancer specimens,so as to provide a novel insight into the epigenetic mechanism involved in BRCA1 transcription. Methods Serous ovarian cancer patients(10 chemosensitive and 10 chemoresistant cancer)were enrolled for the study. BRCA1 levels was analyzed by real?time quantitative PCR. The methylation levels of BRCA1 core promoter(sites 1?4)was determined by pyrosequencing. Regression analysis was used to examine the possible relationship between BRCA1 levels and the methylation levels of sites 1?4 in ovarian cancer specimens. Results Compared to chemosensitive ovarian cancer tissues,BRCA1 levels were increased,but the methylation levels of BRCA1 core promoter(sites 1?4)were decreased in chemoresistant ovarian cancer tissues. How?ever,it is interesting to note that only a significant inverse correlation was observed between BRCA1 levels and the methylated levels of site 4 (r=-0.612,P<0.05). Conclusion Our findings imply that the methylation levels of site 4 in the core promoter of BRCA1 may be widely involved in the regulation of BRCA1 expression and chemosensitivity in ovarian cancer.

Diabetic peripheral neuropathy is a common complication of diabetes mellitus,can occur in some patients with painful diabetic peripheral neuropathy.The occurrence of PDPN is related to many factors,such as long term severe hyperglycemia and metabolic disorders,deficiency of neurotrophic factors, disorder of microcirculation,increase of oxygen free radicals in oxidative stress and disorder of immune func-tion.Mitogen activated protein kinase (MAPK)is a kind of serine /tyrosine protein kinase ,which can widely expressed in cell,and can be involved in the pathophysiological process of stress,inflammation,cell cycle and apoptosis through increased nuclear transcription factors.P38MAPK signaling pathway is an im-portant branch of the MAPK,this overview focuses on the research progress of p38MAPK signaling pathway in the pathogenesis of PDPN.

Dendritic cells are now given more attention th ese years in tumor immuno-therapy. In this review, we summarized the advances o f dendritic cells vaccines and their applications in malignant tumors such as me lanoma and renal carcinoma.