Objective:To establish an HPLC method for the determination of clindamycin phosphate in compound phenytoin sodi-um ge1s. Methods:The HPLC analysis was carried out on a ZORBAX SB-C18 column(250 × 46 mm,5 μm)with 0. 1 mol·L-1 KH2PO4 solution(adjusting pH to 2. 5 with H3PO4 solution)-acetonitrile(75:25)as the mobile phase at the flow rate of 0. 8 ml· min-1 . The detection wavelength was 210 nm,the column temperature was 25℃ and the injection volume was 10μl. Results:The lin-ear range of clindamycin phosphate was 3. 00-18. 00 μg(r=0. 999 5). The average recovery was 101. 11%(RSD=0. 34%,n=6). Conclusion:The method is simple,sensitive and reproducible,and can be used in the determination of clindamycin phosphate in com-pound phenytoin sodium gels.

Mechanisms of perineural invasion may include the effect of microenvironment, the interaction of cancer cell and nerve, the action of stromal cell and cancer cell, signal transduction between the cancer cell and nerve by the involvement of the neurotrophin and its receptor, chemokines and its receptor.Its impacts on early cervical cancer include surgery, adjuvant therapy, prognosis and so on.

The bilingual teaching must face some innovations and reformations in the contents and methods to acquire some satisfactory outcome.The article discusses some questions of bilingual teaching in paediatrics including the shortage of teachers,the interest deficiency of students,the lack of suitable teaching materials and inferior learning environment,and then provides some useful advice for the innovations of bilingual teaching.

Objective To investigate the effect of Danzhijiangtang capsule on serum,fat in type 2 diabetic rats.Methods The model of type 2 diabetes mellitus rats were established by injecting small does streptozotocin(STZ)into abdomen and feeding high sucrose-fat diet.All the rats were divided randomly into five groups.The level of blood glucose,serum insulin,insulin sensitivity index,blood fat(triglyceride, cholesterol,high-density lipoprotein,low-density lipoprotein),free fatty acid after treated were examined. Results Danzhijiangtang capsule could decrease the level of blood fat.Conclusion Danzhijiangtang capsule could improve the blood fat in type 2 diabetic rats.

Objective To compare the accuracy of quantitative CT(QCT), perfusion scintigraphy and anatomical segmentation in predicting postoperative lung function in lung cancer patients. Methods Pulmonary functional tests, quantitative CT scan and perfusion seintigraphy in 12 cases before operation were performed. Forced vital capacity (FVC), the first second forced expiratory volume (FEV1.0) and diffusing capacity of carbon monoxide (DLco) were obtained from preoperative pulmonary functional tests. According to the corresponding formula for QCT, perfusion sintigraphy and anatomical segmentation method, the values of FVC, FEV1.0 and DLco were predicted. The correlation between the predicted values and postoperative values of FVC, FEV1.0 and DLco were assessed. The paired-t test,Pearson correlation test and Bland-Altman analysis were used for the statistics. Results The predicted values of QCT, perfusion sintigraphy and anatomical segmentation method were: FVC [(3.05±0.82), (2.98±0.75) and (2.98±0.86) L,respectively] , FEV1.0[(2.20±0.81), (2.17±0.78) and (2.16±0.84) L, respectively], DLco (FVC: r=0.87, 0.80 and 0.86; FEV1.0:r =0.93, 0.91 and 0.93; DLco:r =0.93, 0.95 and 0.93,respectively,P < 0.01). Conclusion QCT, perfusion sintigraphy and anatomical segmentation method can be used in predicting postoperative lung function. The predicted values are in concordance with the postoperative ones.

Acupuncture Therapy ; Child, Preschool ; Female ; Hand, Foot and Mouth Disease ; complications ; Humans ; Infant ; Male ; Paraplegia ; etiology ; therapy

OBJECTIVE To establish the quality control system of aseptic materials in sterilization and supply department. METHODS According to Antisepsis Technic Criterion,Criterion of Check and Accept of Sterilization and Supply Department in Hospital,and Criterion of Hospital Infection newly issued by National Ministry of Health,to provide quality control of aseptic meterials in hospital. RESULTS The aseptic materials of quality control were improved,and come to being systematic standardized. CONCLUSIONS The improved quality control is an important measure for the guarantee of quality control of aseptic materials.

Evidence-based medicine is advocated by WHO and adopted by developed countries for many years. In China, however, the selection of essential medicine and various medical insurance reimbursement schemes medicine is usually based on experts' experience of prescription practice which is under heavy critics resulting from the lack of related comparative efficacy and evidence-based research. The efficacy of Jian'ganle in prevention of drug-induced liver injury (DILI) caused by antituberculotics was evaluated in this study by comparison with Hugan Pian, glucuronolactone and reduced glutathione. Evidence was provided for relevant sectors such as Ministry for Human Resources and Social Security of the People's Republic of China and National Health and Family Planning Commission of the People's Republic of China to select and renew the Essential Medicine List (EML), the new rural cooperative medical scheme in China (NRCMS) list or the reimbursement list of industrial injury insurance. A total of 189 patients with initial pulmonary tuberculosis were divided into four groups who took antituberculotics combined with Jian'ganle, Hugan Pian, glucuronolactone and reduced glutathione respectively. Their liver function profile including alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), direct bilirubin (DBIL), total protein (TP), albumin (A) and globulin (G) were detected at admission as baseline and after treatment. The Jian'ganle group was compared with the three others by chi-square tests. In an aspect of maintaining bilirubin indexes normal, Jian'ganle was more efficacious than glucuronolactone. And Jian'ganle had a little more efficacy than reduced glutathione to maintain protein indexes normal as well. And the therapeutic regimen of antituberculotics combined with Jian'ganle was the best in treating tuberculosis and preventing DILI at the same time. The study showed that among the four hepatinicas which demonstrated similar prevention of DILI caused by antituberculotics, Jian'ganle has more advantages over the three others to some extent, which provides a reliable basis for health sectors to select and renew the EML, NRCMS List or the reimbursement list of industrial injury insurance.

Objective To investigate the effect of intermittent hypoxia on neuronal apoptosis and inducible nitric oxide synthase (iNOS) expression in rat hippocampus,in order to explore the potential mechanism of hippocampal neuronal apoptosis induced by intermittent hypoxia.Methods Twenty-four Wistar male rats (280-350 g) were randomly divided into three groups:the normal control,intermittent normoxia and intermittent hypoxia group (n=8 each).The animal model of intermittent hypoxia was established by an automated nitrogen/oxygen profile system.The three groups were respectively exposed to continuous normoxia (21 ％O2),cyclical normoxia (21 ％O2),and cyclical hypoxia [alternating between normoxia (21 ％ O2) and hypoxia (5 ％ O2),every 120 seconds] throughout the eight hours of light time(8:00-16:00).The rats were dissected and the hippocampus was removed at the end of the designated duration of exposures for six weeks.TdT-mediated dUTP nick end labeling (TUNEL) was used to detect the apoptotic rate of the hippocampus region in each group.Reverse transcription-PCR,immunohistochemistry (IHC) and Western blotting were used to examine mRNA and protein expressions of iNOS in the hippocampus tissue.Results The apoptotic rate of hippocampal neurons was higher in intermittent hypoxia group than in intermittent normoxia group [(28.236±0.081) ％ vs.(9.341±0.026)％,P＜0.05].The mRNA and protein expressions of iNOS were higher in intermittent hypoxia group than in intermittent normoxia group (3.394± 1.344 vs.0.125±0.040,7.793±0.052 vs.1.356±0.039,both P＜0.05].Conclusions Intermittent hypoxia can induce hippocampal neuronal apoptosis in rats,accompanied by the upregulation of iNOS gene transcription and protein expression,which indicates that iNOS may involve in the process of hippocampal neuronal apoptosis induced by intermittent hypoxia.

The article analyzes patients' needs from humanistic psychology to find out shortages existing in current medical service.The values and importance of medical humanistic care are also discussed from the perspective of medical humanism.