Histone deacetylase 6 (HDAC6) is an unique subtype of histone deacetylases with two tandem deacetylase domains and substrate specificity for non-histone proteins. It is involved in many important physiological and pathological processes and has become a promising therapeutic target in recent decades. Different kinds of potent HDAC6-selective inhibitors have been reported around the world. This paper reviews the progress in the study of structure and functions of HDAC6 as well as the development of HDAC6-selective inhibitors.

AIM: To separate and determine berberine type alkaloids in Huanglian Shangqing Pills(tablets)(Rhizoma Coptidis,Fructus Gardeniae,Fructus Forsythiae,Cortex Phellodendri Chinensis,etc.). METHODS: RP-HPLC was used,and the mobile phase consisted of acetonitrile-solution of potassium phosphate(3.4 g?L~(-1),adjust pH to 3.0 with H_3PO_4)(40∶60),with 1.7 g?L~(-1) SDS.The detecting wavelength was at 345 nm. RESULTS: The contents of five kinds of berberine type alkaloids,berberine,palmatine,coptisine,epiberberine and jatrorrhizine in Huanglian Shangqing Pills(tablets) produced by 10 pharmaceutical factories were determined. CONCLUSION: The method is simple,accurate with a good recovery and repeatability.The method can be used to control the quality of Huanglian Shangqing Pills(Tablets).

How to acquire new tumor antigens is a hotspot in cancer research currently.According to the nature of antigen epitope binding its ligand,we can identify and get unknown antigen epitope even its amino acid sequence through some analytical techniques and experiments,from T lymphocytes and monoclonal antibody levels.So the molecular mechanisms of tumor immunity will be more distinct.

Pleomorphic adenoma (PA) is the most common benign tumor of the salivary glands. Originating from the nasal cavity is very rare. This paper reports one case of pleomorphic adenoma of the inferior nasal turbinate to analyze the clinic characteristic of this disease. Although these tumors are rarely seen in everyday practice, one should consider this possibility as an uncommon aetiology when confronted with an intranasal mass.
Adenoma, Pleomorphic ; pathology ; Humans ; Nasal Cavity ; Nose Neoplasms ; pathology ; Paranasal Sinuses ; Turbinates ; pathology

Histone deacetylase 6 (HDAC6) is an unique subtype of histone deacetylases with two tandem deacetylase domains and substrate specificity for non-histone proteins. It is involved in many important physiological and pathological processes and has become a promising therapeutic target in recent decades. Different kinds of potent HDAC6-selective inhibitors have been reported around the world. This paper reviews the progress in the study of structure and functions of HDAC6 as well as the development of HDAC6-selective inhibitors.
Histone Deacetylase Inhibitors ; pharmacology ; Histone Deacetylases ; chemistry ; Humans ; Substrate Specificity

The paper aims to explore the studying method for the pharmacokinetics of drugs in target organs, the pharmacokinetic process of tramadol hydrochloride in the extracellular fluid of frontal cortex (FrCx) of mice was investigated. Six male mice (Kunming strain) were anaesthetized (urethane, 1.8 g x kg(-1), ip) and secured on a stereotaxic frame. A microdialysis probe was implanted into the FrCx and perfused with artificial cerebrospinal fluid at a flow rate of 2 microL x min(-1). One hour later, mice were administrated (ip) with tramadol hydrochloride (50 mg x kg(-1)) and dialysates were collected continuously at 12-min intervals (24 microL each) for 6 h. The tramadol concentration in dialysates was determined by HPLC-Ultraviolet detection method, and the concentration-time curve and pharmacokinetic parameters of tramadol were calculated with DAS software. The results showed that the pharmacokinetic process of tramadol in the FrCx extracellular fluid of mice was fitted to a two-compartment open model, and the main pharmacokinetic parameters t1/2alpha, t1/2beta, t(max), C(max) and AUC(0-infinity) were (0.27 +/- 0.05) h, (2.72 +/- 0.24) h, (0.50 +/- 0.10) h, (2 110.37 +/- 291.22) microg x L(-1) and (4 474.51 +/- 441.79) microg x L(-1) x h, respectively. In conclusion, a studying method for pharmacokinetics of drugs in the target organ is established, which is simple and feasible. Tramadol hydrochloride shows a two-compartment model in the extracellular fluid of the mouse FrCx, and the distribution- and elimination half-life are 0.5 h and 2.7 h, respectively.

AIM: To establish quality standard for prepared pieces of Rhizoma Panacis Majoris. METHODS: Traditional identification and TLC were used for qualitative analysis of prepared pieces. The contents of ginsenoside Ro and chikusetsusaponin IVa were determined by HPLC. Water,ash and acid-insoluble ash were also detected. RESULTS: The average content of ginsenoside Ro was 7. 19% ,chikusetsusaponin IVa was 3. 16% from different habitants. CONCLUSION: The established method has been proved to be simple,stable,accurate,and can be applied to the determination of ginsenoside Ro and chikusetsusaponin IVa in Rhizoma Panacis Majoris. The quality control standards for Rhizoma Panacis Majoris is normative and the result is accurate.

Objective To construct the pcDNA3. 1-Brugia malayi (Bm) glyceraldehyde-3-phosphate dehydrogenase (GAPDH) eukaryotic recombinant plasmid and to study its effect on mouse cellular immunity response. Methods Total RNA was prepared from periodic Bm. The target gene fragments were amplified by reverse transcription-polymerase chain reaction (RT-PCR) technique and then were inserted into the cloning vector. pGEM-T Easy, and sub-cloned into pcDNA3. 1. Purified pcDNA 3. 1-BmGAPDH recombinant plasmid and CpG were injected into the anterior tibial muscle of BALB/c mice in order to induce host immunity response. Mice injected with PBS and mice injected with blank plasmid were prepared as controls. The mouse models were immunized for 3 times with an interval of 2 weeks. RT-PCR was utilized to detect target gene expression in the muscle tissue. MTT method was used to measure the immunized mice T lymphocytes stimulation index, while enzyme-linked immunoassay (ELISA) was used to determine the serum interleukin (IL)-4 and interferon (IFN)-γ level. Means were compared using t test with SPSS software. Results The recombinant plasmid pcDNA3. 1-BmGAPDH was constructed sucessfully. The target gene was 1020 bp long and its homology with known gene sequence in database was 99%. BmGAPDH gene in the injected muscle of the immunized mice was detected by PCR. The proliferation of spleen T lymphocytes was higher in pcDNA3-BmGAPDH group than in the 2 control groups which were 1. 398, 1. 006 and 1. 017,respectively (P< 0. 05). The levels of IFN-γ and IL-4 in serums from the immunized mice were significantly higher than those of the PBS control group and blank plasmid control group which were 163.905, 58.589, 51. 317 and 107. 906, 27.111, 34.627, respectively (P<0. 05). Immune adjuvant CpG could accelerate and boost antigen-specific immune responses induced by vaccine, which presented as significant increase of IFN-γ and lymphocyte proliferation at 4 weeks after immunization.Conclusion The recombinant eukaryotic plasmid pcDNA3. 1-BmGAPDH is constructed and could elicit cellular immune responses in immunized mice.

Objective To investigate the effect of Nifekalant on action potential of atrium and pulmonary vein and the possible clinical efficacy of Nifekalant against atrial tachyarrhythmias.Methods left and right atrium and pulmonary vein removed from 15 white rabbits were made into tissue preparations(0.5 cm?1.5 cm).Action potential was induced by electric stimulation with twofold capture threshold and recorded by standard glass microelectrode technique.Effective refractory periods of left and right atrial and pulmonary vein tissues were measured with burst and programmed stimulation.The Action potentials and effective refractory periods of various tissues were compared between before and after irrigation of Tyrodes fluid containing Nifekalant of 2.13 mg/L.Results After irrigation of Tyrodes fluid containing Nifekalant,APD90 was prolonged[(51?16)ms vs(78?33)ms,P

Objective To investigate the lipidemia of patients with coronary artery disease(CAD)in China.Methods The survey engaged 52 centers in 7 cities in China recruiting patients with coronary artery disease in a hospital-based setting.Each center was asked to recruit all hospitalized patients with CHD from 1-Jun-2005 to 31-Sep-2005.The study population consisted of 3513 patients with CAD.Results Of the 3513 patients,the prevalence of the lipidemia was 79.7%.And the prevalence of the lipidemia in