Fatal Outcome ; Humans ; Infant ; Intracranial Hemorrhages ; etiology ; Male ; Siblings ; Vitamin K Deficiency ; complications

Targeted therapy is the most prospective part of the research related to the treatment of non-small cell lung cancer(NSCLC). After epidermal growth factor receptor(EGFR),anaplastic lymphoma kinase (ALK)gene is expected to become a new target in the treatment of NSCLC. Moreover,several related resear-ches suggest that ALK inhibitor(crizotinib)is effective for treatment of ALK-rearranged NSCLC. Therefore,it is important to review the detection method of ALK gene,clinicopathologic features and the research progress of ALK inhibitor for the individual treatment of NSCLC.

Adult ; Automobile Driving ; Female ; Health Status ; Humans ; Male ; Middle Aged ; Occupational Health ; Young Adult

Objective Toevaluatetheeffectsofcombinedintracoronaryandintravenous administrationofnicorandil onmyocardialmicrocirculationandshort-termprognosisinpatientswithacuteST-segmentelevationmyocardialinfarction (STEMI)treatedwithprimarypercutaneouscoronaryintervention(PPCI). Methods Atotalof100patientswithacute STEMIunderwentPPCIwererandomlydividedintothenicorandilgroup(patientsreceivedintracoronarybolusinjectionof nicorandilwhenthetargetvesselopenedandthencontinuousintravenousinfusionwithin24hours, n=50)andthecontrol group(patientsreceivednormalsalineascontrol, n=50).Themainoutcomemeasureswereimmediatecoronaryflowand myocardialperfusionafterPPCI,includingthrombo-Lysisinmyocardialinfarction(TIMI)flowgrade,correctedTIMIframe count(CTFC),reperfusionarrhythmia,ST-segmentresolution,plasmacreatinekinaseisoenzyme(CK-MB)peakvalueand time. The secondary indicators were major adverse cardiovascular events (MACE) and left ventricular ejection fraction (LVEF)duringhospitalization.Results Therewerenosignificantdifferencesinhepatorenalfunction,heartrateandblood pressurebeforeandafteroperationineachgroup(P＞0.05).Theincidenceofreperfusionarrhythmia,thelevelofMACE, CTFC,andpeakvalueofCK-MBwereallsignificantlylowerinthenicorandilgroupcomparedwiththoseofcontrolgroup (P＜0.05).TheproportionsofpatientswithTIMI3flow,CK-MBpeaktimein14hours,andtheproportionofST-segment resolutionweresignificantlyhigherinthenicorandilgroupthanthoseofthecontrolgroup(all P＜0.05).Therewasno significant difference in LVEF during hospitalization between two groups (P＞0.05). Conclusion Intracoronary and intravenousadministrationofnicorandilcansignificantlyimproverevascularizationeffects,reducetheoccurrenceofslow flow/noreflow,limitmyocardialinfarctionsize,increasemyocardialperfusionandimprovemyocardialmicrocirculationand theshort-termprognosisofacuteSTEMIpatients.

Enterovirus A, Human ; pathogenicity ; Enterovirus Infections ; complications ; Humans ; Pulmonary Edema ; etiology ; virology

Angelicae Sinensis Radix, dried roots of Angelicae sinensis, is a well-known traditional Chinese medicine. Six compounds were isolated and purified by silica gel and preparative HPLC. According to physicochemical properties and spectral data, the compounds were identified as senkyunolide H-7-acetate (1), o-phthalic acid (2), diisobutyl phthalate (3), p-hydroxyphenylethanol ferulate (4), ferulic acid (5) and coniferylferulate (6). Compound 1 was a new one.
Angelica sinensis ; chemistry ; Benzofurans ; chemistry ; isolation & purification ; Coumaric Acids ; chemistry ; isolation & purification ; Dibutyl Phthalate ; analogs & derivatives ; chemistry ; isolation & purification ; Drugs, Chinese Herbal ; chemistry ; Medicine, Chinese Traditional ; Plant Roots ; chemistry

Objective To establish a method for determining cinnamaldehyde in Zegui Longshuang Capsules. Methods A C18 column was used with Acetonitrile-Water (30∶70) as the mobile phase,and the detection wavelength was at 290 nm. Results The calibration curve of cinnamaldehyde was linear from 0.010 ?g to 0.507 ?g,and r=0.999 9. The average recovery was 98.55 %with RSD being 2.06 %. Conclusion This method is proved to be accurate and reliable,and can be used to control the quality of Zegui Longshuang Capsules.

Chromatography ; methods ; DNA Fingerprinting ; Drugs, Chinese Herbal ; analysis ; Magnetic Resonance Spectroscopy ; Mass Spectrometry

Objective Poly (ADP-ribose) polymerase (PARP) have been demonstrated to play an important role in systemic lupus erythematosus (SLE). In this study we assessed the expression of PARP on peripheral blood mononuclear cells with active or inacte SLE and tried to investigate the relationship between PARP and cell apoptosis on SLE. Methods Thirty definitive SLE patients and 10 healthy controls were enrolled. PBMC were separated from the peripheral blood samples. Western blot technique was applied to analyze the expression of PARP. Flow cytometry were applied to analyze the cell apoptosis. T test were used. Results The cell apoptosis in active patients with SLE was significantly higher than that of inactive patients with SLE and normal controls (the t values were 4.83 and 5.05 respectively, P<0.05). The level of PARP expression was significantly decreased in active patients with SLE as compared with controls and inactive patients with SLE (the t values were 7.66 and 7.07 respectively, P<0.05). At the same time, the cleavage fragment of PARP increased in active patients. Conclusion The results suggest that PARP may be involved in the pathogenesis of SLE and the expression level may be a good indicator for disease activity of SLE . Decrease of PARP may promote the occurrence and development of SLE. In addition, PARP may play a certain role in adjusting cell apoptosis in patients with SLE.

Objective To establish a mathematical model to describe the skeletal class Ⅲ malocclusion of patient dental and basal bone arch form, for providing a data reference and basis for further study. Methods Thirty-five patients with skeletal classⅢmalocclusion were selected in this study for computed tomography CBCT. The data of 3-D image were analyzed, and dental arch marker (Fa) and base bone arch marker (Ba) were determined. The reference plane was determined by least square method. Software Matlab 7.0 was used to calculate two-dimensional coordinate system. Based on this, a mathematical model was established to describe the dental and basal bone arch form and then to validate the mathematical model. Results (1) The mathematical model can be used to describe the dental arch form of skeletal classⅢmalocclusion, maxillary:Y=46.12 [1-(2X/70.99)2]1.052;mandibular:Y=39.16 [1-(2X/64.51)2]1.038. (2) The mathematical model can be used to describe the basal bone arch form of skeletal classⅢmalocclusion, maxillary:Y=43.14 [1-(2X/75.09)2]1.061;mandibular:Y=39.03 [1-(2X/60.63)2]1.021. (3) Fa was located at Ba labial side in the maxilla, the distance was positive. Fa was located at Ba lingual side in the mandibular, and the distance was negative. (4) The fitting correlation coefficient of beta-function curve and each tooth on the dental and basal bone arch of skeletal class Ⅲ malocclusion were greater than 0.7 (P<0.05). Conclusion In this study, the mathematical model can be used to describe the dental and basal bone arch form of the skeletal classⅢmalocclusion, which can guide further research.