Objective To study the effects of aerobic exercises on platelet function in patients with phase Ⅰ essential hypertension (EH). Methods Low-intensity aerobic exercises therapy was given to 36 patients with phase Ⅰ EH for three months. Changes of their blood pressure, heart rate, platelet aggregation rate (PAGTmax), alpha-granule membrane protein (GMP-140) and mean platelet volume (MPV) werer ecorded and compared to those in 36 normal healthy controls. Results As compared to those in controls,PAGTmax, level of GMP-140 and MPV were all increased in patients with phase Ⅰ EH before exercises therapy(t=15.221, 10. 916 and 16. 401, respectively, all P < 0. 01 ). After exercises therapy, 24-hour mean blood pressure decreased in association with reduction in PAGTmax to (0. 80±0. 05)% from (0. 88±0. 08) % (t=4. 323, P < 0. 05), level of GMP-140 decreased to ( 28±20) μg/L from (57±23 ) μg/L ( t=15.049, P<0.01) and MPV decreased to (9.0±0.9)fl from (9.1±0.9)fl (t=5.518, P <0.05) before it. But, there was no significant difference in these in dicators of platelet function in controls before and after exercise therapy (P>0.05). Conclusions Aerobic exercises therapy could be effective in lowering blood pressure in patients of mild EH with increased platelet activation, as well as inhibition ontheir platelet activation.

Objective To evaluate the effect of community health education on pre-hospital delay and clinical outcomes of hospitalization of the patients with acute myocardial infarction.Methods Community health education for the adults living near the Linyi People's Hospital was conducted from July 1st to October 30th,2012.ST-elevation acute myocardial infarction identified 1 year prior to or during the health education program was used as control (n=81) or study group (n=89),respectively.Delay from the onset of ST-elevation acute myocardial infarction symptoms to hospital admission,the rate of reperfusion therapy,the in-hospital malignant complications and the death rate were compared between the two groups.Student's t test,Chi-square test,Fisher's exact probability test and the Wilcoxon rank sum test were used for data analysis.Results Median pre-hospital delay was 80 (47-150) min in the study group,less than 90 (60-255) min of the control group (Wilcoxon W=6 912.00,Z=-2.182,P=0.029).The rate of reperfusion therapy was 54％ in the study group,higher than 38％ of the control group (x2 =4.181,P=0.041).The overall incidence of severe complications during hospitalization was 24％ in the study group,less than 42％ in the control group (x2=6.732,P=0.009).Conclusion Community health education could decrease pre-hospital delay for acute myocardial infarction,improve the rate of reperfusion therapy and decrease the overall incidence of severe complications during hospitalization.

[Objective]To introduce the effect of treatment of recurrent dislocation of the patella with reconstruction of MPFL in children.[Method]Forteen knees of 11 cases of children recurrent patellar dislocation were treated by reconstruction of MPFL,the patients were followed for 3 years at least.The rate of recurrence,the subjective symptoms and the function of the injured leg were evaluated.[Result]There was no recurrence at the last follow up.The IKOC subjective knee scores were(39.4?4.7)and(91.3?6.1)(P

Objective To investigate the change of heart rate variability(HRV) in patients with white coat hypertension (WCH).Methods Participants were classified as essential hypertension (EH) group (n=35),white coat hypertension (WCH) group (n=35),normotension (NT) group (n=35) based on clinic and 24 h ambulatory blood pressure monitoring.The time and frequency-domain measures of HRV were measured by 24 h ambulatory electrocardiographic recording.Results Compared with NT controls,the standard deviation of normal-to-normal intervals (SDNN),rate-mean-square of the difference of successive RR intervals (RMSSD) and high frequence (HF) in the patients with EH and WCH were significantly decreased[SDNN:NT(132.2?21.5)vs EH(115.9?18.9)vs WCH(120.6?17.5)ms;RMSSD:NT(36.8?9.3)vs EH(28.6?8.9)vs WCH(27.5?8.5)ms;HF:NT(206.8?99.7)vs EH(159.5?86.6)vs WCH(161.1?84.5)ms2/Hz;all P0.05).Conclusion These changes in the parameters of HRV showed cardiac autonomic nerve dysfunction in white coat hypertensive patients.

Objective To investigate the role of glycogen synthase kinase-3β (GSK-3β) in the trafficking of NR1-containing N-methyl-D-aspartate receptor (NMDAR) and NR2B-containing NMDAR in the spinal cord in rats with incisional pain (IP) and remifentanil-induced hyperalgesia.Methods Twenty-four male Sprague-Dawley rats,aged 2-3 months,weighing 240-260 g,were randomly divided into 3 groups ( n =8 each):control group (group C),remifentanil group (group R) and GSK-3β inhibitor TDZD-8 group (group TDZD-8).A 1-cm longitudinal incision was made in the plantar surface of the left hindpaw in anesthetized rats.In group C,dimethyl sulfoxide (DMSO) 2 ml/kg was injected and then the normal saline (equal to the volume of remifentanil) was infused for 60 min via the caudal vein.In group R,DMSO 2 ml/kg was injected before IP was made and then remifentanil was infused at a rate of 1.2 μg· kg- 1 ·min- 1 for 60 min.In group TDZD-8,TDZD-8 2 ml/kg was injected before IP was made and then remifentanil was infused at a rate of 1.2 μg·kg-1 ·min-1 for 60 min.Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were measured 24 h before infusion of normal saline or remifentanil and at 2,6,24 and 48 h after intravenous injection.The rats were sacrificed after the last measurement of the pain threshold and L4-6 segment of the spinal cord was removed to determine the expression of NMDAR NR1 and NR2B subunits in cell membrane (s) and cytoplasm (i) in the spinal cord by Western blot analysis.The ratios of sNR1/iNR1 and sNR2B/iNR2B were calculated.Results Compared with group C,PWT was significantly decreased and PWL was significantly shortened,the expression of sNR1 and sNR2B was up-regulated,the expression of iNR1 and iNR2B was down-regulated,and the ratios of sNR1/iNR1 and sNR2B/iNR2B were significantly increased in groups R and TDZD-8 (P ＜ 0.05).Compared with group R,PWT was significantly increased and PWL was significantly prolonged,the expression of sNR1 and sNR2B was down-regulated,the expression of iNR1 and iNR2B was up-regulated,and the ratios of sNR1/iNR1 and sNR2B/iNR2B were significantly decreased in group TDZD-8 ( P ＜ 0.05).Conclusion GSK-3β is involved in the regulation of trafficking of NR1-containing NMDAR and NR2B-containing NMDAR from cytoplasm to cell membrane in the spinal cord in rats with IP and hyperalgesia induced by remifentanil.

Acanthosis Nigricans ; diagnosis ; genetics ; pathology ; Adolescent ; Antigens, CD ; genetics ; Biomarkers ; blood ; DNA Mutational Analysis ; Donohue Syndrome ; diagnosis ; genetics ; pathology ; Humans ; Insulin Resistance ; genetics ; Male ; Mutation ; genetics ; Polymerase Chain Reaction ; Receptor, Insulin ; genetics ; Sequence Analysis, DNA

Actins ; metabolism ; Adult ; Diagnosis, Differential ; Endometrial Neoplasms ; metabolism ; pathology ; surgery ; Endometrial Stromal Tumors ; metabolism ; pathology ; surgery ; Female ; Humans ; Hysterectomy ; Leiomyoma, Epithelioid ; metabolism ; pathology ; Melanoma-Specific Antigens ; metabolism ; Perivascular Epithelioid Cell Neoplasms ; metabolism ; pathology ; surgery ; Receptors, Progesterone ; metabolism ; Sarcoma, Clear Cell ; metabolism ; pathology ; Uterine Neoplasms ; metabolism ; pathology ; surgery

Acupuncture Points ; Adult ; Electroacupuncture ; Female ; Head ; innervation ; Humans ; Neuritis ; therapy

Animals ; DNA Methylation ; Epigenesis, Genetic ; Histones ; metabolism ; Humans ; Neoplasms ; genetics ; pathology ; Neoplastic Stem Cells ; metabolism ; pathology ; RNA, Untranslated

Background:Small bowel Crohn's disease (CD) is a chronic nonspecific inflammatory disease with unknown etiology.There is no clear criteria for assessing the progression of CD, and the endoscopic index for assessing disease activity in CD patients is limited to a single parameter.Aims:To investigate the clinical value of endoscopic index in evaluating disease activity in patients with CD and the correlation between different parameters.Methods:Sixty cases of CD patients form Jan.2008 to Dec.2012 at Affiliated Hospital of Yanan Uuiversity were collected, 60 healthy adults were served as control group.CECDAI score, CDAI score and Lewis score were used to evaluate disease activity in patients with CD, and their relationship with CRP and ESR were analyzed.Results:Levels of CRP and ESR were significantly increased in CD patients than in controls (P<0.05).CDAI score and Lewis score were statistically different in different CECDAI score groups (F=5.53, P<0.05;F=22.64, P<0.05).CECDAI score was statistically different in different CDAI score groups (F=3.55, P<0.05), and was statistically different in different Lewis score groups (F=4.26, P<0.05).CECDAI score was correlated with CDAI score, Lewis score, CRP, ESR (P<0.05);CDAI score was correlated with CRP, ESR (P<0.05) and not correlated with Lewis score (P>0.05);Lewis score was not correlated with CRP, ESR (P>0.05) in CD patients.Conclusions:CECDAI score, CDAI score, Lewis score, CRP, ESR can reflect disease activity of CD, and can be used together to assess disease activity of CD patients.