1.Clinical Significance of M K and VEGF Expression in Recurrent Gastric Carcinoma
Zhong JIA ; Guanghua FENG ; Qi XIE ; Xin FANG
Journal of Medical Research 2006;0(05):-
Objective To investigate the correlation between midkine(MK) and VEGF expression with angiogenesis and biological features in recurrent gastric carcinoma.Methods The expression of MK was examined using immunohistochemistry in 9 cases of gastric carcinoma and 9 cases of adjacent normal tissues.Microvessel density(MVD)was determined by VEGF immunohistochemical staining.Results Majority of recurrent gastric carcinoma tissues showed positive reaction to immunostaining,but no specific positivity was detected in adjacent normal tissue.Values of MVD and MK positive group(88.8%,79.75) was higher than those in the negative group(15.0%)(P
2.Exploration of PBL in medical microbiology teaching
Fang FANG ; Xin LIU ; Lan LIU ; Dongmei CHEN ; Lan WANG ; Yi WU ; Jia XU
Chinese Journal of Medical Education Research 2011;10(9):1059-1061
The PBL education about the chapter of TB in microbiology for medical English-class students was conducted.The results was analyzed by questionaire,showing that PBL helps to stimulate students' learning enthusiasm and improve the comprehensive ability and enhance the teachers' awareness of the responsibility.
4.Host-schistosome interactions mediated by cross-species microRNA
ZHANG Jing⁃yu ; QIN Ming ; FANG Jing-jing ; LI Jia-yi ; ZHANG Xin-xin ; ZHOU Fang-bin ; HE Xing
China Tropical Medicine 2022;22(12):1201-
Abstract: Schistosomiasis is a serious major parasitic disease that threatens human life and health. A better understanding
of the mechanism of host-schistosome interactions is the key to designing new prevention and control strategies. MicroRNAs
(miRNAs) are endogenous small non-coding RNA molecules, which lead to the degradation of the target messenger RNA
(mRNA) or inhibition of its translation in a sequence-specific manner. Both schistosome and its host produce miRNAs, which
can be secreted by extracellular vesicles (EVs). There is accumulating evidence that miRNAs from schistosome can be taken
up by host cells, and finely manipulate the phenotype of host cells for their survival or pathogenesis in a cross-species manner,
even inhibiting the growth and metastases of hepatoma cells. It is still unknown whether host free miRNAs can be taken up by
schistosome, but this phenomenon is highly probable. miRNA-mediated cross-species regulation has emerged as a novel
mechanism for host-schistosome interactions, and this review summarizes the advances in this regard.
5.Recent advances in small-molecule inhibitors targeting influenza virus glycoproteins
Yuan-min JIANG ; Ji-wei ZHANG ; Rui-fang JIA ; Hui-nan JIA ; Ying ZHANG ; Xin-yong LIU ; Peng ZHAN
Acta Pharmaceutica Sinica 2023;57(11):3254-3269
Hemagglutinin and neuraminidase, two important glycoproteins on the surface of influenza virus, play a considerable role in the entry and release stage of the viral life cycle, respectively. With in-depth investigation of influenza virus glycoproteins and the continuous innovation of drug discovery strategies, a new generation of glycoproteins inhibitors have been continuously discovered. From the point of view of medicinal chemistry, this review summarizes the current advances in seeking small-molecule inhibitors targeting influenza virus glycoproteins, hoping to provide valuable guidance for future development of novel antiviral drugs.
6.Hypoxic preconditioning up-regulates the activity and gene expressions of glucose transporters (GLUT1 and GLUT3) in cultured rat hippocampal neurons and astrocytes under anoxic condition
Erfu WANG ; Xin LI ; Chunsong JIA ; Guangwei LIU ; Haixia FANG ; Shun YU
Basic & Clinical Medicine 2009;29(12):1273-1276
Objective To explore the effect of hypoxic preconditioning on the activity and gene expressions of glu-cose transporters in the cultured rat hippocampal neurons and astrocytes under anoxic condition. Methods The cultured rat hippocampal neurons and astrocytes were treated for 6 days by intermittently exposing to hypoxic gas mixture (1% O_2, 10% CO_2, 89% N_2) for20 min each day. 24 h after the last hypoxic exposure, the cells were exposed to anoxic gas mixture (10% CO_2, 90% N_2) for 6 h, and the uptake rate of [~3H]-2-deoxyglucose (2-DG), the levels of glucose transporter GLUT1 and GLUT3 mRNAs and the cell survival rate were examined im-mediately after anoxic exposure. Results Neurons and astrocytes preconditioned with hypoxia showed higher 2-DG uptake rates and increased expressions of GLUT 1 mRNA in the astrocytes and GLUT 1 and GLUT 3 mRNA in the neurons. The preconditioned neurons also showed an increased tolerance to anoxia. Conclusion Hypoxic precon-ditioning up-regulates the activity and gene expressions of glucose transporters of hippocampal neurons and astro-cytes under anoxic condition.
7.The effects of balloon dilatation on swallowing dysfunction in patients with dysphagia
Wei-Hong QIU ; Zu-Lin DOU ; Gui-Fang WAN ; Jia-Xuan LIN ; Jie-Xin LIN ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(12):-
Objective To study the effect of balloon dilatation therapy on dysphagia caused by cricopharyn- geal achalasia.Methods Ten cases of dysphagia were diagnosed as cricopharyngeal achalasia by videofluoroscopic swallowing study(VFSS).A 14~* urethral catheter was inserted into the esophagus and an amount of water was injec- ted into the balloon of the urethral catheter to make it turgid.Then the catheter was pulled upwards and passed through the stricture of esophagus to dilatate the cricopbarygeus muscle.Meanwhile,low frequency electrical stimula- tion was used and combined with functional training of the organs related to deglutition and ingestion.The results be- fore and after the treatment were evaluated.Results After 19.7 times of dilatation therapy,the content of water in- jected into the balloon was increased from 2.65?0.91 ml to 8.20?0.92 ml.Cricopharyngeal achalasia was alle- viated significantly(P
9.Melanotic oncocytic metaplasia of the nasopharynx.
Bao-cheng DONG ; Hao TIAN ; Xin-qi JIA ; Gelin LI ; Cheng LU ; Yankun ZHANG ; Xiaoming ZHU ; Fang YU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(7):549-550
Aged
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Female
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Humans
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Male
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Melanosomes
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Metaplasia
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Middle Aged
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Nasopharynx
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cytology
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pathology
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Oxyphil Cells
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cytology
10.Isolation and identification of triterpenoids from root of Achyranthes bidentata in Henan.
Shu-ping JIA ; Zhi-yong YU ; Zhi-fang HAO ; Jian-xin LI
China Journal of Chinese Materia Medica 2006;31(15):1244-1247
OBJECTIVETo investigate the triterpenoids from root of Achyranthes bidentata in Henan.
METHODSephadex, normal-and reversed-phase column chromatographies were applied for the isolation and purification. The structure determinations were performed by means of physiochemical properties, MS and NMR data analyses.
RESULTSeven compounds were isolated from the water soluble fraction in root of A. bidentata, and determined as achyranthoside A (1), achyranthoside E (2), momordin Ib (3), chikusetsusaponin IVa (4), chikusetsusaponin IVa methyl ester (5), chikusetsusaponin V (6), chikusetsusaponin V methyl ester (7).
CONCLUSIONCompounds 1 and 2 were isolated from the natural resources for the first time.
Achyranthes ; chemistry ; Molecular Conformation ; Molecular Structure ; Oleanolic Acid ; analogs & derivatives ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Saponins ; chemistry ; isolation & purification