Objective To explore the effects of electrical stimulation below the contraction threshold on apoptosis and the expression of caspase-3 in cardiomyocytes after myocardial infarction in rats.Methods Thirty-two rats were randomly divided into 4 groups:a sham-operated group,a myocardial infarction group,an ischemic electrical stimulation group and a non-ischemic electrical stimulation group.TdT-mediated X-dUTP nick end labeling (TUNEL)was used to investigate apoptosis of cardiomyocytes.Reverse transcription-polymerase chain reaction(RTPCR)and immunohistochemical techniques were used to detect the expression of caspase-3.Results ①Compared with the sham-operated group,the amount of apoptotic cardiomyocytes increased significantly in the two electrical stimulation groups and the ischemic myocardium group.Compared with the ischemic myocardium group,apoptotic cardiomyocytes decreased significantly in the two electrical stimulation groups.There was no significant difference between the two electrical stimulation groups.②Compared with the sham-operated group,in the ischemic myocardium group and the two electrical stimulation groups,expression of caspase-3 increased significantly.Compared with the ischemic myocardium group,expression of caspase-3 decreased significantly in the two electrical stimulation groups.There was again no significant difference between the two electrical stimulation groups.Conclusions Electrical stimulation below the contraction threshold can reduce ischemic cardiomyocyte apoptosis in rats.The mechanisms may be associated with down-regulation of the expression of caspase-3.Electrical stimulation below the contraction threshold of the myocardium can also reduce cardiomyocyte apoptosis in rats.

Antigens, Neoplasm ; genetics ; metabolism ; Breast Neoplasms ; enzymology ; genetics ; metabolism ; DNA Topoisomerases, Type II ; genetics ; metabolism ; DNA-Binding Proteins ; genetics ; metabolism ; Female ; Gene Expression Regulation, Neoplastic ; physiology ; Humans

Cell Movement ; drug effects ; Chemokine CXCL12 ; genetics ; metabolism ; pharmacology ; physiology ; Humans ; Neoplasm Invasiveness ; physiopathology ; Neoplasm Metastasis ; physiopathology ; Neoplasms ; metabolism ; Neovascularization, Pathologic ; physiopathology

Carcinoma ; drug therapy ; genetics ; metabolism ; pathology ; radiotherapy ; Desmoplastic Small Round Cell Tumor ; metabolism ; pathology ; Diagnosis, Differential ; Gene Rearrangement ; Head and Neck Neoplasms ; drug therapy ; genetics ; metabolism ; radiotherapy ; Humans ; Keratin-20 ; metabolism ; Keratin-7 ; metabolism ; Lymphatic Metastasis ; Male ; Mediastinal Neoplasms ; drug therapy ; genetics ; metabolism ; radiotherapy ; Melanoma ; metabolism ; pathology ; Neuroectodermal Tumors, Primitive ; metabolism ; pathology ; Nuclear Proteins ; genetics ; metabolism ; Oncogene Proteins ; genetics ; metabolism ; Oncogene Proteins, Fusion ; genetics ; metabolism ; Rhabdomyosarcoma ; metabolism ; pathology ; Thymus Neoplasms ; drug therapy ; genetics ; metabolism ; radiotherapy

Aged, 80 and over ; Amyloid beta-Peptides ; metabolism ; Brain ; pathology ; Cerebral Amyloid Angiopathy ; complications ; metabolism ; pathology ; Cerebral Hemorrhage ; etiology ; pathology ; Consciousness Disorders ; etiology ; pathology ; Humans ; Male

Objective To explore the value of liver biopsy in diagnosis of the severity of chronic hepatitis B (CHB).Methods The liver biopsy, laboratory examination and imaging were performed in 176 CHB patients. The clinical and histological diagnoses were compared. Biochemical indexes of liver function were also compared between patients with histological inflammation activity ＜G2 and≥G2 or the patients with fibrosis score ＜S2 and ≥S2. Rank sum test and χ2 test were used in univariate analysis. Binary Logistic regression was used in multivariate analysis. Results Among 176patients, the clinical diagnosis of CHB and cirrhosis were established in 171 patients and five patients,respectively. Among 171 CHB patients, the clinical diagnosis was consistent with histological diagnosis in 102 (59.6%) patients. The clinical diagnosis was more severe than histological diagnosis in 60 (35.1%) patients and the clinical diagnosis was less severe than histological diagnosis in nine (5.3%) patients. Among 44 patients with histological grade G2, 41 (93.2 %) patients had alanine aminotransferase (ALT)≥2 × upper limits of normal (ULN). According multiple Logistic regression analysis, age, overt symptom, ALT level, cholinesterase (CHE), albumin (Alb), total bile acid (TBA) and ultrasonic appearance of non-smooth liver surface were independent risk factors for the diagnosis of fibrosis score ≥S2. Conclusions The CHB diagnosis based on clinical evidences doesn't show acceptable consistency with the diagnosis based on histological evidences. G2 and S2 may be more reliable criteria for diagnosing moderate and severe CHB.

Objective To measure von Willebrand factor(vWF)concentrations and von Willebrand factor-cleaving protease(vWF-cp)activity in plasma of lung cancer patients and to evaluate their clinical significance.Methods The vWF:antigen(vWF:Ag)and vWF-cp activity were measured by using ELISA and residual-collagen binding assay(R-CBA),respectively in 78 patients with lung cancer.The serum and pleural fluid(PLF)of carcino-embryonic antigen(CEA)were measured by radioimmunity assay in 23 patients.Results (1)The level of vWF:Ag in lung cancer patients(107.7?43.7)%was significantly higher than that in benign diseases(71.3?49.5)% and normal controls(82.4?41.3)%(P

Objective To investigate the effects of sodium butyrate (SB) on fetal gastric epithelial cells (GECs) in vitro damaged by N-methyl-N’-nitro-N-nitrosoguanidine (MNNG). Methods The fetal gastric epithelial cells were isolated, cultured and identified. Then the cells of the second generation were cocultured with 10 -5 mol/L, 10 -6 mol/L, 10 -7 mol/L SB for 4 h, then added with 10 -5 mol/L MNNG culturing for another 24 h. After the treated cells were finally cultured in normal culture medium for 40 d, the unscheduled DNA synthesis (UDS), lipid peroxidation (LPO) and ras p21 were detected. The cells were only cultured with 10 -5 mol/L MNNG served as negative control and without MNNG or SB as normal control. Results The level of UDS, the contents of lipid peroxidation products and the concentrations of ras p21 in GECs treated with 10 -5 mol/L and 10 -6 mol/L SB decreased obviously, and had significant differences with that of GECs treated without SB. Conclusion SB can effectively prevent the damage of GECs induced from MNNG.

Objective To explore the division XL automatic urine analyzer reagent strips placed time impact on nitrite (NIT) test results,to ensure that provide accurate and reliable experimental data for clinical,and improve clinical diagnostic rate. Methods Used a new load reagent storehouse article in the new reagents respectively in <5,30 min;1,2,3,3.5,4,4.5,5, 5.5,6,6.5,7 and 8 h,in different period,16 0.9 g/dl saline NIT in the false positive rate,and carried on the comparison to different times NIT false-positive rate analysis.Results <3.5 h NIT false positive rate was 0% (0/16),4~4.5 h false pos-itive rate was 18.8% (3/16),5~5.5 h false positive rate was 43.8% (7/16),6~6.5 h was 62.5% (10/16),7~8 h was 87.5% (14/16).More than 4 h each time comparison between NIT false positive rate,differences were statistically signifi-cant (χ2 =11.7~59.2,all P <0.01).Conclusion Division XL automatic urine analyzer reagent strips placed after 4 h,NIT false-positive rate started to rise,NIT false-positive rate was significantly increased after 5 h.Therefore,strengthen the divi-sion XL automatic urine analyzer reagent strips placed reasonable time management,should be in the < 3.5 h advisable,it helps reduce the NIT false positive rate.

Objective To understand red blood cells glucose-6-phosphate dehydrogenase (G6PD)dificiency of pregnancy cou-ple in baoan district of Shenzhen,in order to draw the attention of society.Methods Collected 6 574 cases of couples of child-bearing age (3 192 males and 3 382 females),in Baoan District People’s Hospital,from February to November 2013. Venous blood was collected using EDTA anticoagulant blending respectively,the improvement of red bloodcells and glucose-6-phosphate dehydrogenase quantitative ratio method to determine anticoagulant G6PD/6 PGD ratio in the whole blood,with ratio <1.0 convicted of G6PD deficiency.Results 3 192 cases of male subjects,G6PD deficient 176 cases,3 382 cases of pregnant women client detection of 135 cases in the detection rate were 5.51% and 3.99%,respectively,the total detection rate was 4.73% (311/6 574),G6PD lack of rate was higher than that of pregnant women,men G6PD lack of male∶female was 1.30∶1 (176∶135),the difference was statistically significant between (χ2 =7.16,P <0.05).Conclusion The baoan district couple G6PD lack had a higher incidence of pregnancy,men were significantly higher than women,should arouse at-tention.Couples pregnancy test during pregnancy should be advocated the G6PD activity screening,the eugenics and mater-nal and child health care has important significance.