1.The effects of subthreshold electrical stimulation on apoptosis and caspase-3 expression of ischemic cardiomyocytes
Chinese Journal of Physical Medicine and Rehabilitation 2011;33(11):808-811
Objective To explore the effects of electrical stimulation below the contraction threshold on apoptosis and the expression of caspase-3 in cardiomyocytes after myocardial infarction in rats.Methods Thirty-two rats were randomly divided into 4 groups:a sham-operated group,a myocardial infarction group,an ischemic electrical stimulation group and a non-ischemic electrical stimulation group.TdT-mediated X-dUTP nick end labeling (TUNEL)was used to investigate apoptosis of cardiomyocytes.Reverse transcription-polymerase chain reaction(RTPCR)and immunohistochemical techniques were used to detect the expression of caspase-3.Results ①Compared with the sham-operated group,the amount of apoptotic cardiomyocytes increased significantly in the two electrical stimulation groups and the ischemic myocardium group.Compared with the ischemic myocardium group,apoptotic cardiomyocytes decreased significantly in the two electrical stimulation groups.There was no significant difference between the two electrical stimulation groups.②Compared with the sham-operated group,in the ischemic myocardium group and the two electrical stimulation groups,expression of caspase-3 increased significantly.Compared with the ischemic myocardium group,expression of caspase-3 decreased significantly in the two electrical stimulation groups.There was again no significant difference between the two electrical stimulation groups.Conclusions Electrical stimulation below the contraction threshold can reduce ischemic cardiomyocyte apoptosis in rats.The mechanisms may be associated with down-regulation of the expression of caspase-3.Electrical stimulation below the contraction threshold of the myocardium can also reduce cardiomyocyte apoptosis in rats.
2.Effect of CXCL12 in tumor microenvironment.
Fang-Fang LIU ; Jia WEI ; Li FU
Chinese Journal of Pathology 2008;37(3):193-196
3.Topo II alpha in breast cancer: an update.
Jia WEI ; Fang-fang LIU ; Li FU
Chinese Journal of Pathology 2008;37(2):132-135
4.Midline carcinoma with rearrangement of nuclear protein in testis gene.
Chinese Journal of Pathology 2011;40(3):209-212
Carcinoma
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drug therapy
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genetics
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metabolism
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pathology
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radiotherapy
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Desmoplastic Small Round Cell Tumor
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metabolism
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pathology
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Diagnosis, Differential
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Gene Rearrangement
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Head and Neck Neoplasms
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drug therapy
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genetics
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metabolism
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radiotherapy
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Humans
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Keratin-20
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metabolism
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Keratin-7
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metabolism
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Lymphatic Metastasis
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Male
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Mediastinal Neoplasms
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drug therapy
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genetics
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metabolism
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radiotherapy
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Melanoma
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metabolism
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pathology
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Neuroectodermal Tumors, Primitive
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metabolism
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pathology
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Nuclear Proteins
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genetics
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metabolism
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Oncogene Proteins
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genetics
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metabolism
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Oncogene Proteins, Fusion
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genetics
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metabolism
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Rhabdomyosarcoma
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metabolism
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pathology
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Thymus Neoplasms
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drug therapy
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genetics
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metabolism
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radiotherapy
5.Sudden consciousness disorders.
Wei ZHANG ; Fang LIU ; Dong-ge LIU
Chinese Journal of Pathology 2011;40(9):632-634
6.Application of MRI cranial surface locating device in localization of small intracranial space-occupying lesions
Youpei WEI ; Xiguang LIU ; Fang LI
The Journal of Practical Medicine 2014;(7):1027-1029
Objective To explore the feasibility of applying MRI cranial surface locating device in localization of small intracranial space-occupying lesions before the surgeries. Methods Thirty patients with small intracranial space-occupying lesion were localized by MRI cranial surface locating device before the surgeries. The clinical accuracy was verified during the surgeries and the results were compared with cases without using the device. Results According to the verifications during the surgeries , 30 cases which used MRI cranial surface locating device showed much higher accuracy than that using the traditional method. Conclusions MRI cranial surface locating device can precisely delineate the surgical target area. The device is easy to operate and also can help to design the rational surgical approach with minimum injuries , significantly improve accuracy of locating small intracranial space-occupying lesions and it is safe , effective and worthy of widely used clinically.
7.An experimental study on the expression of heme oxygenase-2 mRNA in Hirschsprung's disease.
Min, ZHU ; Mingfa, WEI ; Fang, LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2002;22(2):152-4
In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the aganglionic and ganglionic segments of colon respectively from 15 cases of HD. The single-stranded cDNA of HO-2 was synthesized and further amplified by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HO-2 mRNA was normal in ganglionic segments, but absent in aganglionic segments. It is concluded that the absence of HO-2 mRNA expression may be an important mechanism responsible for HD.
Colon/enzymology
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Heme Oxygenase (Decyclizing)/biosynthesis
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Heme Oxygenase (Decyclizing)/*genetics
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Hirschsprung Disease/*enzymology
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Hirschsprung Disease/etiology
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Hirschsprung Disease/genetics
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RNA, Messenger/biosynthesis
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RNA, Messenger/genetics
8.Value of transvaginal ultrasonography and color Doppler ultrasonography in diagnosis of postmenopausal disorders in uterine cavity
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(09):-
0.05). The pathological findings indicated benign lesions in 63 patients(73%) and malignant lesions in 25 patients(27%)in group 1. All the lesions were found benign in group 2. There was significant difference between the two groups(P=0.0033). Conclusion Noninvasive and painless transvaginal two-dimensional ultrasonograpy and color Doppler ultrasonography may have important clinical application value in diagnosis of intrauterine anomaly in post-menopausal women.
9.Preventive effects of sodium butyrate on gastric epithelial cells damaged by N-methyl-N’-nitro-N-nitrosoguanidine
Wei LIU ; Bin ZHU ; Dianchu FANG
Journal of Third Military Medical University 2003;0(20):-
Objective To investigate the effects of sodium butyrate (SB) on fetal gastric epithelial cells (GECs) in vitro damaged by N-methyl-N’-nitro-N-nitrosoguanidine (MNNG). Methods The fetal gastric epithelial cells were isolated, cultured and identified. Then the cells of the second generation were cocultured with 10 -5 mol/L, 10 -6 mol/L, 10 -7 mol/L SB for 4 h, then added with 10 -5 mol/L MNNG culturing for another 24 h. After the treated cells were finally cultured in normal culture medium for 40 d, the unscheduled DNA synthesis (UDS), lipid peroxidation (LPO) and ras p21 were detected. The cells were only cultured with 10 -5 mol/L MNNG served as negative control and without MNNG or SB as normal control. Results The level of UDS, the contents of lipid peroxidation products and the concentrations of ras p21 in GECs treated with 10 -5 mol/L and 10 -6 mol/L SB decreased obviously, and had significant differences with that of GECs treated without SB. Conclusion SB can effectively prevent the damage of GECs induced from MNNG.
10.Research on electrochemical luminescence analyzer test anemia indicators of performance verification methodology
Wei YAO ; Aisheng LIU ; Duzhi FANG
China Medical Equipment 2016;13(1):74-77
Objective:To detect anaemia parameter methodology performance for validation of Roche Cobas E601 automatic electrochemical luminescence immunity analyzer.Methods:Recommended by the American association of clinical laboratory standardization (CLSI) method was developed for the determination of folic acid, iron, protein, and this precision, accuracy, linear range, sensitivity, biological reference range and carry pollution index, and validated.Results: Cohas E601 determination of folic acid, iron, protein and precision, the daytime in this batch variation coefficient were 3.03%~4.27% and 3.51%~4.68%. Relative bias must lean on(%) between -3.54%~4.46%. The scope of determination of linear range and the manufacturer to provide similar. Folic acid, iron, protein and numerical value with the determination of this instrument manufacturers provide reference interval coincidence rate were 90.0%, 85.0% and 90.0% respectivel. Instrument to detect carry pollution rate is 0.04%~0.16%. CohasE601 detection sensitivity were 0.23 ng/ml, 0.21 ng/ml and 0.19 pg/ml.Conclusion: Cobas E601 detect folic acid, iron, protein and good performance of this methodology, but manufacturers provide biological reference range is not suitable for the local crowd, should establish the corresponding normal reference range.