Some immune responses induced by atherosclerosis may aggravate the state of the illness by inducing persistent arterial inflammation, whereas others may inhibit arterial inflammation and atherosclerosis.Thus, the prevention and treatment of atherosclerosis may be achieved by the method of immunomodulation, by which selectively suppress proatherogenic immune responses or activate antiatherogenic ones.

Objective To investigate nursing cooperation during gastric coronary vein embolization procedure with NBCA glue. Methods Perioperative nuusing experience in 36 hepatic cirrhosis patients complicated with esophagus-gastric fundus vein varicose rupture and hemorrhea who had undergone gastric coronary vein embolization procedure was retrospectively summarized. Results Operations in all 36 cases had been successful,with no severe complication due to inadequate nursing cooperation. Conclusions Gastric coronary vein embolization operation utilizing NBCA glue is a safe and effective method of hemostasis,skilled and elaborative nursing cooperation is an important component ensuring a smooth and effective operation.

Objective To evaluate lactulose breath hydrogen test in diagnosis of small intestinal bacterial overgrowth in children.Me-thods Thirty-five children,aged 3 to 13 years old with mean (7.72?3.06) years old presented with chronic diarrhea,abdominal pain and flatulence.Among them,18 cases were male and 17 cases were female.Breath hydrogen test was carried out after the administration of a 0.5 g?kg-1 or 10 g lactulose,which was 100 g?L-1 solution.After the ingestion of lactulose,the breath hydrogen was collected in every 20 minutes and up to 3 hours.Small intestinal bacterial overgrowth was diagnosed as positive when the hydrogen concentration increased more than 20 mg?m-3 in a sample.Results Eighteen cases in 35 (51.43%) children were positive and 17 cases were negative.Among these positive cases,8 cases in 18 (22.86%) patients were typically double peaks in breath hydrogen concentrations.Among the causes of small intestinal bacterial overgrowth in children,4 cases with chronic superficial gastritis,4 cases with chronic bacterial enteritis,2 cases with malnutrition and anorexia,1 case with volvulus of stomach,1 case with massive resection of small intestine,1 case with steatorrhea and 5 cases with unknown causes.Four cases of chronic bacterial enteritis were gained remission by antimicrobial agents.The other cases were treated by lac-to-and bifidbacteria probiotics.Seven patients of the clinical systems were controlled,3 cases were improved remarkably and 4 cases were failed.Conclusions The lactulose breath hydrogen test is validated as screening test in the diagnosis of small intestinal bacterial overgrowth in children.Antimicrobial agents and bifidbacteria probiotics is effective in treatment of small intestinal bacterial overgrowth.

Benzene ; analysis ; Chemical Industry ; Chromatography, Gas ; methods ; Humans ; Occupational Exposure ; analysis ; Toluene ; analysis ; Trichloroethylene ; analysis ; Xylenes ; analysis

Background Stickler syndrome is a genetic connective tissue disorder that affects the ocular,skeletal,orofacial and auditory systems.To determine the gene mutation loci can offer a basis for genetic diagnosis and management of Stickler syndrome.Objective The aim of this study was to research the clinical characteristics of a pedigree with Stickler syndrome and identify the disease-causing gene mutation.Methods This study was approved by Ethic Committee of Peking Union Medical College Hospital.The clinical study and pedigree analysis were performed in one family with Stickler syndrome type Ⅰ (STL Ⅰ).Nine family members were examined with informed consent.The entire coding regions of COL2A1 gene with flanking intronic regions were amplified by PCR and directly sequenced.The detected sequence change was confirmed to be mutationloci by examining whether they existed in normal control individuals.Mutant proteins were predicted with online software.Results There were 4 generations and 11 members in this family,and 2 members died,including 1 patient.Three patients were found in 9living families.Inheritance of this family complicd with an autosomal dominant inheritance mode.All affected individuals showed the consistent phenotypes with STL Ⅰ,including high myopia,membranous vitreous anomaly and surface central flat,short nose,palatoschisis,etc.Mutation screening of COL2A1 gene revealed that the first base of intron 12 was deleted(IVS12+1G del).Nucleotide sequence analysis showed that this mutation led to the functional abnormal of this gene by forming termination cordon in advance.This mutation occurred in all affected individuals,however,no mutation was observed in any unaffected member or 100 normal unrelated individuals.Conclusions This study identifies a novel splice-site mutation(IVS12+ 1G del)in COL2A1 gene in a Chinese STL Ⅰ pedigree.This is the first report on a mutation in a Chinese STL Ⅰ family.

AlM: To study the effects of different concentrations of Coix seed oil on human retinal capillary endothelial cells ( HRCECs ) proliferation and vascular endothelial growth factor ( VEGF) expression in high glucose environment.
METHODS: HRCECs extracted from human fresher eyeball and cultured in vitro, and ultimately used in the experiment were the growth of 3rd ~ 4th cells, the experimental were divided into blank control group, low glucose control group, high glucose control group, high glucose + ( 50ü L/mL, 100ü L/mL, 200ü L/mL ) different concentrations Coix seed oil group. Detecting the multiplication of HRCECs by MTT, the immunocytochemical method was employed to detect the each group HRCECs of VEGF expression.
RESULTS:MTT assay results showed that: different concentrations of coix seed oil acted at HRCECs for 48h, inhibition of cell proliferation was significant difference compared with high glucose control group (P<0. 05). Within 48h showed concentration dependence. There was no statistical difference between the low glucose group and high glucose control group (P>0. 05). lmmunocytochemical assay showed that:50ü L/mL, 100ü L/mL, 200ü L/mL Coix seed oil acted at HRCECs 48h, the expression of VEGF decreased significantly compared with the high glucose control group ( P< 0. 05 ), and in a dose- dependent manner. However, in high glucose control group, the expression of VEGF was obvious higher than that of low glucose control group (P<0. 05).
CONCLUSlON:Coix seed oil can inhibit the HRCECs proliferation and suppress the VEGF expression in high glucose environment.

AIM and METHODS: Rat myocardial mRNA was extracted and mitochondrial sarcomeric creatine kinase (sMiMi-CK) was used as labled probed in vitro myocardial ischemia/reperfusion rats. Northern blot was performed to determine the changes of sMiMi-CK mRNA in different periods of ischemia/reperfusion and the protective effect of phosphocreatine (PCr).RESULTS:During early ischemial (10 min) mRNA was decreased about 18 24%. After reperfusion mRNA decreased again. PCr effectively prevented the decrease of mRNA expression of sMiMi-CK. CONCLUSION:The results indicated that PCr may possess protective effect on anoxic and reoxygenated myocardium.

BACKGROUND:Polyvinyl alcohol(PVA)has similar water content as human tissues,good biocompatibility and high mechanical strength,thereby being utilized as tissue-engineered matrix material.However,the modification in cellular affinity benefits the function as tissue regeneration scaffold materials.OBJECTIVE:To prepare chitosan(CS)/PVA composite scaffold and explore the feasibility of serving as a scaffold for tissue engineering.METHODS:Scaffolds of CS/PVA was fabricated by blending PVA-124 with various amounts of CS using film forming,particle forming,and free-drying methods.Water content,light transmittance,and swelling ratio were tested.Scanning electron microscope was used to observe the configuration of the cross section.RESULTS AND CONCLUSION:A total of 7 different proportional scaffolds were obtained with different PVA and CS dosage.The composite film showed light transmittance between 70%-80%,high water content between 121.2%-162.5% and swelling ratio between 60.3%-133.7%.Composite particle and sponge obtained water content between 82.0%-461.2% and 280.8%-1 939.0%.Composite scaffold with the amount ratio of PVA 0.75 g/CS 0.15 g exhibited the best performance.Scanning electron microscope showed that at a mass ratio of PVA 0.75 g/CS 0.15 g,the scaffold prepared by freeze-drying method had regular,fluffy inner structure,with good mechanics and high water content.

BACKGROUND: Polyvinyl alcohol (PVA) has similar water content as human tissues, good biocompatibility and high mechanical strength, thereby being utilized as tissue-engineered matrix material. However, the modification in cellular affinity benefits the function as tissue regeneration scaffold materials. OBJECTIVE: To prepare chitosan (CS) / PVA composite scaffold and explore the feasibility of serving as a scaffold for tissue engineering. METHODS: Scaffolds of CS/PVA was fabricated by blending PVA-124 with various amounts of CS using film forming, particle forming, and free-drying methods. Water content, light transmittance, and swelling ratio were tested. Scanning electron microscope was used to observe the configuration of the cress section. RESULTS AND CONCLUSION: A total of 7 different proportional scaffolds were obtained with different PVA and CS dosage. The composite film showed light transmittance between 70%-80%, high water content between 121.2%-162.5% and swelling ratio between 60.3%-133.7%.Composita particle and sponge obtained water content between 82.0%-461.2% and 280.8%-1 939.0%. Composite scaffold with the amount ratio of PVA 0.75 g/CS 0.15 g exhibited the best performance. Scanning electron microscope showed that at a mass ratio of PVA 0.75 g/CS 0.15 g, the scaffold prepared by freeze-drying method had regular, tluffy inner structure, with good mechanics and high water content.

Objective To study the molecular mechanism of the remission in children with acute lymphocyte leukemia(ALL),as well as the expression of thyroid hormone receptor interacting protein 3(TRIP3) gene in children with ALL and explore the relationship between TRIP3 and ALL.Methods Fasting venous blood 2-4 mL was collected,anticoagulanted with ethylenediamine tetraacetic acid(EDTA),then perpheral blood mononuclear cells was collected by Ficoll density gradient centrifugation,total RNA was extracted by Trizol one step method.Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect TRIP3 expression in peripheral blood lymphocytes in 73 ALL children of different stages and 20 normal children.The relationship between TRIR3 expression in children and ALL release was analyzed.Results 1.Expression of TRIP3 was significantly lower both after initial treatment and during recrudescence than that in normal children(Pa0.05).3.In children with ALL after initial treatment,the remission rate was significantly higher in TRIP3 positive patients than in TRIP3 negative ones(remission rate discern 25.0% vs 84.2%,P