Sleep disorders are main clinical symptoms in perimenopausal women. The symptoms are difficulty in initiating or maintaining sleep, early morning awakening and so on. These sleep problems had made serious im-pacts on the quality of women's life during menopausal periods. Menopausal sleep disorders can be caused by changes of serum estrogen levels, vasomotor symptoms, mood disorders and patient's state of disease. This paper presented an overview of inducing factors and current situation of menopausal disorder treatment.

Objective To study the clinical characteristics, diagnosis and treatment of childhood visceral leishmaniasis. Methods A retrospective analysis of 46 cases of children with visceral leishmaniasis was performed, epidemiological and clinical characteristics, laboratory and special inspection, diagnosis and treatment were analyzed. Results All 46 cases (27 males and 19 females) were mostly from the epidemic area and sporadically happened in spring, autumn, and winter, lacking of specific symptoms. Clinical features include long-term and repeated fever, anemia, abdominal distention and respiratory infection, the most common feature is fever accompanied with liver, spleen and lymph node enlargement. Laboratory tests were mainly found with anemia, pancytopenia in peripheral blood , damaged liver function, and elevated CRP. Bone marrow cytology smear found duchenne body- in 26 cases, the positive rate was 61.36%. Visceral leishmaniasis serological antibody were positive in 28 patients, positive rate was 96.29% (28/30). 30 cases were treated with meglumine antimoniate, 26 cases were cured, 3 cases died, and 1 case were not cured and discharged from the hospital. The treatment effective rate was 86.67%. Conclusions Childhood visceral leishmaniasis is still popular in recent years lacking of characteristic clinical symptom. Therefore, for the patients in the epidemic area who presented with long-term fever, anemia, or pancytopenia in peripheral blood, hepatosplenomegaly (especially the splenomegaly), and liver function damage, bone marrow puncture, visceral leishmaniasis antibodies and/or RK39 dipstick test and relevant auxiliary examination should be considered for early diagnosis and treatment, thus to reduce the complications and to improve the cure rate.

Objective: To investigate the impact of levosimendan on mortality in patients with severe heart failure (HF) by Meta-analysis. Methods: We search the PubMed, EMBASE and Cochrane Central Registry of cardiovascular disease to identify all randomized impact of levosimendan vs other medications. The document retrieval was from the establishment of each database until 2014-07. The literatures were taken based on Jadad scale standard and the qualified control study was used without dose and time restrictions by Rev Man 5.2 soft ware, and a total of 37 articles with 4470 patients were finally enrolled for Meta-analysis. Results: Compared with controlling medications, levosimendan could decrease the mortality in patients with cardiac disease caused severe HF (RR: 0.85; 95% CI 0.75-0.97;P=0.02), and cardiac surgery caused severe HF (RR: 0.49; 95% CI 0.28-0.85;P=0.01). Compared with dobutamine, levosimendan could reduce the mortality in patients with severe HF (RR: 0.84; 95% CI 0.73-0.99;P=0.02) and severe ischemic HF (RR: 0.85; 95% CI 0.73-0.99;P=0.04). Conclusion: Levosimendan may reduce the mortality in patients with severe HF caused by cardiac disease, cardiac surgery and ischemic cardiac injury.

Objective To study the psychological intervention of patients with schizophrenia using olanzapine treatment. Methods A total of 336 patients with schizophrenia from February 2015 to October 2016 were selected as the study subjects,randomly divided into the control group and the experimental group,each with 168 patients.Control group patients with olanzapine treatment,on the basis of the experimental group patients given psychological intervention,pay attention to the psychological state of the patients,strengthen the communication and exchanges with the patient,the treatment of patients with increased confidence and treatment compliance.The therapeutic effect of the experimental group and the control group was compared. Results After the corresponding treatment,7 patients in the experimental group showed a nursing dispute,which was 4.17％. In the control group, 46 patients had nursing disputes, accounting for 27.38％.However,the probability of nursing dispute in the experimental group was significantly lower than that in the control group,with statistical difference(P<0.05).The effective number of patients in the experimental group was 168.The effective number of patients in the control group was 120,and the proportion was 71.43％,with statistical difference(P<0.05). Control patients satisfaction with nursing cases for 130 cases,38 cases of patients are not satisfied,70 patients were generally satisfied with,is satisfied with 60 patients,patient satisfaction and nursing satisfaction rate of 77.38％ significantly lower than that of the experimental group(100％),with statistical difference(P<0.05). Conclusion Psychological intervention with olanzapine in the treatment of refractory schizophrenia better clinical effect, can significantly improve the treatment efficiency,further promotion of clinical significance.

Medical nutrition therapy (MNT)is the basic and long-term treatment of diabetes patients. At present,the dietary structure of type 2 diabetes mellitus(T2DM)patients in China has yet to be further improved. This article focused on the effect,dietary structure,food selection and guarantee of compliance of MNT in T2DM.We recommended that the dietary pattern should be designed based on the traditional dietary habits around the country,combined with people living and working reality. The rational utilization of network platform can improve the efficiency and compliance of nutrition education.

Objective To investigate the expression and clinical significance of RhoC protein in breast cancer.Methods Expression of RhoC protein was detected by immunohistochemlstry in 112 cases of breast cancer,20 cases of adjscent non-cancerous normal tissues and 15 cases of normal mammary gland tissues.The relation between expression of RhoC protein and clinicopathological characteristics was analyzed.Expression of CD34 in breast cancer tissues was detected.Microvessel density(MVD)was calculated and its correlation with RhoC protein Was analyzed.Results RhoC had a positive expression rate of 40.2%(45/112)in breast cancer,while it was not expressed in adjacent non-cancerous tissues or normal mammary gland tissues.Rhoc expression in breast tissues was related to lymph node metastasis and pathological stages(P<0.05).MVD value in patients with positive RhoC expression was significantly higher than that in patients with negative RhoC expression(P<0.05).Conclusions The expression of RhoC protein is related to lymphatic metastasis,pathological stages and MVD value.The upregulation of RhoC protein expression may promote the metastasis and invasion of breast cancer.

Objective To study the effect of IL-6 and IL-8 on the MMP-9 secretion of endometrial stromal cells in women with endometriosis to explore the function of IL-6 and IL-8 in the adherence and planting process of ectopic endometrium. Methods The endometrial stromal cells were cultured in DMEM/F12 medium containing different concentrations of IL-6 or IL-8 for 24 hours, then the media were collected to measure the levels of MMP-9 by ELISA. Results The levels of MMP-9 in control stromal cells cultured with high concentration of IL-6 were significantly higher than those without IL-6 (P

Morphine antagonist naloxone (3mg/kgiv ) induced a rise in blood pressure,pulse pressure & respiratory rate in the rat under hemorrhagic shock. It also improved the survival rate. ?Receptor antagonist pheno-xybenzamine ( 1 mg/kg iv ) or adrenalectomy abolished the beneficial effects of naloxone. Reserpine ( 1 mg/kg ip for 5 d) which depleted catecholamines of periphal sympathetic nervous system could not abolish naloxone effects. Resrpine plus adrenalectomy abolished naloxone actions again. It is suggested that the effect of naloxone on the blood pressure is mainly due to release of catecholamine from adrenalmedu-llary. The results of isolated adrenal perfusion indicated that naloxone could not induce a rise in catecholamine concentration of perfused liquid. It is suggested that the action of naloxone on catecholamine release may not be a result of direct action upontbe chromaffin cells.

Objective To reproduce the human endometriosis (EMS) in nude mouse, and to asssess the effects of endostatin in this model. Methods Endometrium tissue was collected from four women undergoing hysterectomy for EMS, and it was transplanted into 30 female BALB/c nude mice by subcutaneous injection (n=20) and intraperitoneal injection (n=10) respectively. The development of endometriotic lesions in nude mice was observed three times every week. Two weeks later, the successful rate in the two groups was estimated. The model mice in subcutaneous injection group were then randomized into treatment group (n=8) and control group (n=7). The mice in treatment group were injected with human endostatin (2mg?kg -1 ?d -1 ), while the mice in control group received an equivalent volume of PBS. All mice were sacrificed 14 days after treatment. Immunohistochemistry was used to determine the expression of vascular endothelial growth factor (VEGF). Results Transplantation was successful in 7 nude mice in abdominal implantation group and 15 nude mice in subcutaneous injection group. The success rate was 75% in subcutaneous injection group and 70% in abdominal implantation group, no significant difference was found between two groups. The level of VEGF in the nude mice treated with endostatin was significantly lower than that in the control group (P