Objective To observe the feasibility of phase-rectified signal averaging applied for the evaluation of automatic nervous system activity in general anesthesia.Methods Forty-seven fe-males and 63 males aged 18-65 years,ASA falling into category Ⅰ or Ⅱ,scheduled for oral maxillo-facial surgery under general anesthesia were selected.The heart rate variability(HRV)signal was re-corded by Powerlab throughout the operation,and then it was sectioned into three segments with 5 minutes:pre-anesthesia(T0 ),means the waking state before the operation;intra-anesthesia(T1 ),the period of operation in 25-30 min;post-anesthesia(T2 ),the consciousness recovery state in the end of the operation.All indicators of HRV analysis was calculated in the time domain.SpO 2 ,MAP and BIS were recorded synchronously in the three periods.Results Compared with T0 ,DC, RMSSD, SDNN,SD1,SD2,logTP,logLF,logHF were significantly lower at T1 and T2 (P <0.05).In con-trast to T1 ,HR,MAP and BIS were significantly higher at T2 (P <0.05 ),DC,RMSSD,SDNN, SD1,SD2,logTP,logLF,logHF were significantly increased at T2 (P <0.05 ).There was positive relation between DC and RMSSD,SD1,logHF (r =0.905,0.909,0.932,respectively,P <0.01). Conclusion As 5 min-DC could indicate the character of PRSA,especially its specificity in showing vagus nerves activities,it is feasible that DC is used to observe the autonomic nerve activity intra op-eration.

In the article the effect of thyroid hormones was reviewed on cardiac ion channels and transports, so as to explain the changes of the action current on cardiac myocyte. Based on the molecular biology, the hormonal regulation of cardiac and transports were discussed and explained.

Objective: To compare the diagnostic value of two-dimensional time-of-flight (2D TOF) magnetic resonance venography in the sagittal plane, 2D TOF in the coronal plane, and three-dimensional contrast-enhanced magnetic resonance venography (3D CE-MRV) for intracranial venous sinus disease. Methods: Thirteen patients were suspected of having intracranial venous system abnormalities in the Neuro-surgical Outpatient Department, Xuanwu Hospital, Capital Medical University were included from July 2011 to March 2012. 2D TOF and 3D CE-MRV scan in the sagittal and coronal planes were performed at the same time. Maximum intensity projection (MIP) was used for 3D reconstruction. The diagnostic results were compared with DSA. The sensitivity, specificity, positive predictive value, negative predictive value and Kappa value of the 8 main vein structures (superior sagittal sinus, straight sinus, bilateral transverse sinus, bilateral sigmoid sinus and bilateral transverse-sigmoid sinus junction) of the 2D TOF in the sagittal plane, 2D TOF in the coronal plane, and 2D TOF in the sagittal plane in combination with coronal plane were calculated. Results: Circled digit oneCompared to the diagnostic results of DSA, there was significant difference in using the diagnosis with TOF in the sagittal plane or coronal plane alone (all P <0.05), but there was no significant difference after combining the two methods (χu2 =1.500, P>0.05). Circled digit twoTaking DSA as a standard, the consistency of 3D CE-MRV and DSA conclusions was the highest (P<0.01, Kappa =0.933). The sensitivity, specificity, positive predictive value, and negative predicting value were 94.4%, 98.8%, 94.4%, and 98.8%, respectively. The consistency of the diagnostic results of 2D TOF in the coronal plane in combination with sagittal plane and the DSA conclusions were higher than that of 2D TOF in the coronal plane or 2D TOF in the sagittal plane alone (Kappa values were 0.815, 0.635, and 0.608, respectively). Conclusion: When it is difficult to diagnose intracranial venous sinus disease with 2D TOF in the coronal plane or 2D TOF in the sagittal plane alone, the 2D TOF sequence of other direction should be added, the collaborative diagnosis of both may improve the accuracy of diagnosis. The diagnosis of 3D CE-MRV and DSA showed a high degree of consistency. They may be used as a routine examination for the diagnosis of cerebral venous sinus disease.

Currently endoscopic retrograde cholangiopancreatograhp(ERCP) has been one of the major treatments to diagnose and cure pancreatic and choledochal diseases in many large and medium-sized hospitals.To meet the requirements of the army,ERCP treatment is practiced in field battle X-ray diagnosis vehicle(Model XCY2002-1/200) and has achieved satisfactory effects.The functions of field battle X-ray diagnosis vehicle in model of XCY2002-1/200 are expanded and new access are explored to cure patients with pancreatic and choledochal diseases in the field.

Objective To establish the suspension cell line of Atractylodes lancea and to study the effect of two endophytic fungal elicitors on its essential oil production.Methods The essential oil was extracted by using ultrasonic wave after suspension cell was treated with endophytic fungal elicitors.Then,the determination of four compounds(atractylone,hinesol,?-eudesmol,and atractylodin) was carried out by gas chromatography.Results By testing in various conditions,the suspension cell line with a rapid growth rate was established.Its highest biomass(6.95 g/L) was obtained on day 21.?-Eudesmol was the only detection in the control suspension cell,and its highest content(17.469 ?g/g) was also reached on day 21.The effect of crude elicitors of two endophytic fungi(belong to Cunninghamella sp.and Gilmaniella sp.respectively,named AL4 and AL12) on the cell growth and the production of essentia1 oil were investigated.Overall AL4 elicitor got better effect.When suspension cell of 14-day-old cultures was exposed to AL4 elicitor(carbohydrate 20 mg/L medium) for 7 d,the biomass increased 3.31% over the control,and the four compounds(atractylone: 14.715 ?g/g,hinesol: 28.395 ?g/g,?-eudesmol: 38.794 ?g/g,and atractylodin: 8.310 ?g/g) were all detected.Among them,the content of ?-eudesmol reached 2.22 times as much as the control.Conclusion The cell growth and the accumulation of essential oil of A.lancea could also be promoted by adding crude elicitors of the endophytic fungi AL4 and AL12.

In this paper the causes of deficiency of public welfare were analyzed in medical research,and some strategies were proposed to enhance the public welfare of public health care services according to the cardinal principles of new healthcare reform package in 2009.These were benefit to maintain the public welfare of public medical and health care services,and to reach the goal of medical research served as the clinic and the patients.

Objective　To study the effects of air disinfect ion by three methods. Methods　Air was disinfected by ultraviol et rays, pentadialdchyde and ozone, efficiency of disinfection was eval uated b y three different disinfection methods using synchronous sampling after and b efo re disinfected. Results　The peak of the bacteria background by precussion method was 10 o'clock, by the ballistic method was 9 o'clock and by the petri dish method was 10 o'clock before disinfected. Three disinfect ion methods were significantly different, efficiency of disi nfection of pentadialdchyde was the highest when it was evaluated by the precuss io n method, and recovery of bacteria was 43.4%～55.8% after disinfected 3 h our s; effect of pentadialdchyde was better than that of the ballistic method, and r ecovery of bacte ria was 21.8%～35.7% after disinfected 3 hours; effect of ultra violet ra ys was better than that of the petri dish method, and recovery of bacteria was 4 2.1%～60.6% after disinfected 3 hours. But their differences were not significan t. In t he course of experiment, temperature and relative humidity have a negative impac t on the ultraviolet ray's efficiency and almost have no impact on others. Conclusions　Disinfection method of the ultraviolet rays could be rep laced by disinfection method of pentadialdchyde and ozone.

Health Promotion ; Humans ; Occupational Health

BACKGROUND:Previous studies have found that the basement membrane matrix can induced the chondrogenic differentiation of bone marrow mesenchymal stem cels, but there is a wide gap between its mechanical properties and practical application, and further research is needed. OBJECTIVE:To prepare a suitable matrigel/chitosan scaffold that has appropriate mechanical properties and remarkable bioactivity for cartilage repair. METHODS: We selected genipin as cross-linking agent, and mixed Matrigel with cross-linked chitosan at different ratios (2:1, 1:1, 1:3). Then rat bone marrow mesenchymal stem cels were seeded on different scaffolds and cultured for 14 days. The mechanical properties of materials were measured by DMA. Cel counting kit-8, FDA staining, ELISA kits and Alcian blue staining were used to measure the bioactivity of materials. RESULTS AND CONCLUSION:The storage modulus of scaffolds was raised from 0.48 kPa to 1.78 kPa with increase ratio of chitosan. Cels spread wel in the early period on al scaffolds, and then the cels on the chitosan scaffold showed reduced chondrogenic activity, but cels on the scaffolds with matrigel could maintain chondrogenic differentiation. The matrigel/chitosan scaffold at a ratio of 1:1 had appropriate mechanical properties and higher levels of colagen II and colagen X at 14 days. The prepared matrigel/chitosan scaffold with decent mechanical performance can promote the differentiation of bone marrow mesenchymal stem cels into chondrogenic lineages, which can be used in cartilage tissue engineering.

0 05) between the two groups in the patient′s age, pregnancy age, parities, the cavity volume of uterine, hemoglobin and blood platelet count The bleeding volume was (51 6?17 2) ml for the treated group and (63 3?17 1) ml for the control group ( P 2 months It could decrease the volume of bleeding during the operation and benefit for patients recovering