Autophagy is a physiological process which delivers the mutant cytoplasmic proteins and dysfunctional subcellular organs into lysosomes for degradation to generate fuel in the deficiency conditions. It is mainly classified into macroautophagy, microautophagy and chaperon-mediated autophagy (CMA), as well as the selective autophagy such as mitophagy and aggrephagy. This review mainly introduces the key molecular markers of macroautophagy, CMA and mitophagy.
Autophagy ; Humans ; Lysosomes ; Mitochondrial Degradation ; Molecular Chaperones

Autophagy is a physiological process which delivers the mutant cytoplasmic proteins and dysfunctional subcellular organs into lysosomes for degradation to generate fuel in the deficiency conditions. It is mainly classified into macroautophagy, microautophagy and chaperon-mediated autophagy (CMA), as well as the selective autophagy such as mitophagy and aggrephagy. This review mainly introduces the key molecular markers of macroautophagy, CMA and mitophagy.

The paper described the role of big data for health economy development and the present data mining and integration of health economy management.It pointed out the importance of overall data criteria management,IT system integration management,healthcare data collection,and normalization of data mining and integration process.The authors also recommended new concepts,technology reserve and institutional development,to provide powerful technical support for the macro decision making,budget allocation,performance analysis,and performance appraisal of the health economy.

In this article we reviewed Gastrointestinal disorders (FGIDs) complicated with Psychological problems and proposed the strategies for the intervention and management of FGIDs with Psychological problems,including awareness-cultivating,skill-learning,treatment and follow-up in clinical practice.

OBJECTIVE To study the classification and distribution of the ?-lactamases produced by Escherichia coli in diabetic patients.METHODS To detect the ?-lactamases produced by E.coli in diabetic patients with a multi-disk synergy test.RESULTS Forty-nine strains of 60 strains in our study produced the ?-lactamases with total positive rate of 81.67%,From them 5 strains were producing penicillinases,17 strains producing BSBLs(28.33%),and 27 strains were producing ESBLs(45.00%),no carbapenem-hydrolyzing ?-lactamases were detected.In 27 strains producing ESBLs,the sensitivities to the ceftazidime,aztreonam and cefotaxime were similar.CONCLUSIONS The types of ?-lactamases produced by E.coli in diabetic patients are mainly BSBLs and ESBLs,without carbapenem-hydrolyzing ?-lactamases;the sensitivities to the ceftazidime,aztreonam and cefotaxime are similar.

OBJECTIVE:To investigate the clinical etiology,serum type and drug resistance of lung cancer complicated with pulmonary Haemophilus influenzae (Hi) infection,in order to provide reference for infection prevention and rational drug use. METHODS:Clinical data of 8025 inpatients with lung cancer complicated with pulmonary infection in our hospital from Jan. 2009 to Jun. 2016 were collected,and the clinical etiology of pulmonary Hi infection was analyzed. The slide agglutination method was used for serotyping,nitrocefin slip method was used to detect β-lactamase,K-B method was used for drug sensitivity test,WHO-NET 5.6 software was used to deal with the results of drug sensitivity test. RESULTS:Eleven factors as age,clinical classifica-tion,invasive operation,surgery,radiotherapy and chemotherapy,hospitalization time,use of broad-spectrum antibiotics or hor-mones and other were closely related to pulmonary Hi infection in lung cancer patients(P<0.05 or P<0.01). Among 8025 speci-mens,104 strains of Hi were detected with detection rate of 1.30%. Serum type NTHi accounted for 44.23%. Separable strains were mainly b type(22.12%),followed by f type(17.31%)and a type(11.54%). Among 104 strains of Hi,56 strains of β-lac-tamase were detected with enzyme-producing rate of 53.85%. Drug resistance of 104 strains of Hi to ampicillin,amoxicillin,com-pound sulfamethoxazole and chloramphenicol were all higher than 35%;drug resistance of Hi toβ-lactamase inhibitors,cephalospo-rins,carbapenems,azithromycin and other antibiotics were all lower than 20%. Drug resistance of β-lactamase producing stains to ampicillin,amoxicillin,compound sulfamethoxazole and chloramphenicol were all higher than those of non-producing strains,with statistical significance (P<0.01). There was no statistical significance in drug resistance to other antibiotics between producing stains and non-producing strains(P>0.05). CONCLUSIONS:The clinical etiology of pulmonary Hi infection in patients with lung cancer is complicated. The serum type of the isolate is mainly NTHi;enzyme production situation is not optimistic,but Hi keeps a low rate of drug resistance to most antibiotics. β-lactamase inhibitors,cephalosporins,carbapenems and azithromycin are first choice for the treatment of Hi infections.

BACKGROUND:The methods to remove the smear layer mainly include mechanical, Carisolv chemomechanical, laser, ozone methods and so on. But studies after caries removal are limited. At present, there is no research for the observation of the smear layer after caries removal using Er:YAG laser, Carisolv chemomechanical and traditional dental turbine.
OBJECTIVE:To observe the morphologic changes of the dentin smear layer after treated with the three methods of Er:YAG laser, Carisolv chemomechanical and traditional high speed dental turbine under a scanning electron microscope.
METHODS:Thirty newly removed premolars or molars with moderate caries were divided into three groups, 10 teeth in each group. The bottom surface of the tooth cavity was observed with the naked eyes after caries removal with Er:YAG laser, Carisolv chemomechanical and traditional dental turbine, respectively. Then, the surface was observed using the scanning electron microscope (magnification ×1 000 and ×2 000).
RESULTS AND CONCLUSION:The texture of the tooth cavity was hard in the three groups. After caries removal, the dentin surface of Er:YAG laser group was rough and uneven, and showed a peak-like shape;The dentin surface of Carisolv chemomechanical group was dark and the bottom was flat;The dentin surface of traditional dental turbine group was smooth and bright, and there was an obvious cutting trace. It was found that the surface of Er:YAG laser group did not have the smear layer, and the dentinal tubule was clearly visible. The most dentinal tubules were visible in the Carisolv chemomechanical group, and the surface was covered with a less smear layer. The surface of the traditional dental turbine group was covered with a thick smear layer, and the dentinal tubule was unseen. The differences in dentin smear layer cleanliness were significant between the three groups. These result indicate that Er:YAG laser can effectively remove the smear layer, which is better than Carisolv chemomechanical method and traditional dental turbine. Traditional dental turbine group is the worst among the three methods.

Müller cells, as the special radial glial cells in the retina, span the entire retina, contact with neurons, microvessels and processes in the retina and play a significant role in protecting retinal structure and function.Diabetic retinopathy (DR) is a major ocular complication of diabetic patients.In the progression of DR, diabetic macular edema (DME) is the main cause of vision loss.During the occurrence of DME, the morphological and structural changes of Müller cells including severer swelling and vacuolation of cell bodies, increased apoptosis, and abnormal secretion of cytokines, etc.damage the blood-retinal barrier (BRB), increase the permeability of BRB and accelerate the exudation of subretinal fluid.In addition, Müller cells can disrupt the regulation of K + and water transportation, obstruct the fluid absorption in the subretinal space and promote the formation of DME.However, in the early stage of DR, neurotrophic factors secreted by Müller cells can protect the retina by alleviating retinal edema and protecting optic ganglion cells, suggesting that Müller cells can be used as targets for DME treatment.Therefore, new strategies can be provided by fully exploring the role and mechanism of Müller cells in the formation of DME.In this paper, the mechanism of Müller cells in DME and its protective role in the progression of DME were reviewed.

AIM: To explore the possible anti apoptotic mechanism of ginsenoside Rg1 on MPP + induced cellular apoptosis. METHODS: The apoptosis of SHSY5Y induced by MPP + was observed by AO EB staining. Flow cytometry was used to quantitate mitochondrial transmembrane potential (△?m) and reactive oxygen species (ROS) . Western Blot was used to detect the expression of bcl 2 and bax proteins in SHSY5Y cells. RESULTS: MPP + induced apoptosis in SHSY5Y cells was obviously inhibited by pretreatment with 10 ?mol?L -1 Rg1. Although there was no difference of mitochondrial transmembrane potential between Rg1 pretreated groups and MPP + groups, the level of ROS in Rg1 pretreated groups decreased, the expression of bcl 2 protein increased and expression of bax protein decreased. CONCLUSION: Rg1 protects against MPP + induced apoptosis in SHSY5Y cells and the effect may be attributed to its remove of ROS and its regulation of expression of bcl 2 and bax proteins.