OBJECTIVE:To investigate the factors influencing the drug release of atenolol nasal gel for references of optimization of its formulation.METHODS:The effects of carbopol 941(0.8%,1.0% and 1.2%)which used as the gel stroma,the amount of surface acting agent Tween-80(0,6% and 8%),and absorption enhancer(DM-?-CD vs.?-CD)on the in vitro release of atenolol nasal gel were investigated.RESULTS:All the above-mentioned 3 factors had marked effects on the in vitro release of atenolol nasal gel.The in vitro release of atenolol nasal gel was markedly enhanced by 0.8% carbopol 941,6% Tween-80 and 10% DM-?-CD absorption enhancer.CONCLUSION:The results in the study serve as references for the optimization of the formulation of atenolol nasal gel.

The paper researches the co-authorship situation in the field of foreign medical ethics through the social network analysis method,taking 9 foreign core journals of medical ethics included in the Journal Citation Report as the data source,it extracts and sorts out the information through BICOMB and UCINET software to generate the co-occurrence matrix of the author and draw the map,reveals the overall structural features and co-authorship situation of the co-authorship network in the field of foreign medical ethics.

Objective:To construct expression vector of human glucocorticoid receptor mutant.Methods:cDNA sequence of human glucocorticoid receptor mutant having no LBD was amplificated through RT-nested PCR,PCR product was directionally cloned to pEGFP-N2 plasmid vector,and recombinant plasmids were screened through sequencing.Expressions of recombinant plasmids in human macrophage cell line U937 were observed through fluorescence microscope.Transcriptional activation activity of variant was evaluated through methods of relative activity of luciferase.Results:The 1 601 bp length cDNA was obtained,and result of sequencing confirmed the cloned cDNA sequence is completely coincidence with that of glucocorticoid receptor in Genebank.Expression products of recombinant plasmid mainly located in nucleus.Transcriptional activation activity incresed after recombinant plasmid transfected.Conclusion:Expression vector of glucocorticoid receptor mutant was constructed successfully,which has transcriptional activation activity independent of glucocorticoid.

OBJECTIVE To determine the germicidal efficacy and corrosiveness of San Xiao pyrogen inactivator.METHODS Its germicidal efficacy was measured with carrier test.RESULTS The killing rate of Bacillus subtilis var.niger spores exposed to its solution containing available chlorine 750mg/L for 30 min attained 100%.Its solution containing available chlorine 500 mg/L with a 15 min contact time could destroy HBsAg antigenicity in suspension.Its germicidal efficacy decreased in presence of organic substance.Its water solution containing available chlorine 1000mg/L was not corrosive to stainless steel,moderately corrosive to carbon steel and severely corrosive to copper and aluminum.CONCLUSIONS San Xiao pyrogen inactivator is a kind of original disinfectant which has better germicidal efficacy and uses safely.

Humans ; Leukemia ; Neoplastic Stem Cells ; Stem Cell Research

TCM literature in Qing Dynasty has the characteristic of a huge quantity, a great variety, a complete taxon and an extensive spreading. Of TCM literature in other Dynasties, it has the most quantity and makes the later generation know the development of medicine in Qing Dynasty. TCM literature and development of TCM can promote each other, the development of TCM promote the publish of TCM books, on the other hand, the publish and spreading of TCM books promote the development of TCM.

Objective:To investigate functional changes of AMs to explore the mechanisms for weakened pulmonary local immune defense function in DM.Methods:Wistar rat model was established by intraperitoneal injection of streptozotocin at 60 mg/kg.The rats were performed BAL to collected AMs.Cock RBC phagotosis rate of AM was counted with HE method.AMs underwent in vitro incubation,and supernant TNF-? content was determined with ELISA method.Results:The phagotosis rate of 4 weeks DM group and 8 weeks DM group decreased significantly in statistics commpared with the control group(P

OBJECTIVE:To investigate the possible mechanism of the neuroprotective action of isatin(ISA).METHODS:A total of 40 C57BL/6J mice were divided into 4 groups:control group,model group,ISA(200 mg?kg-1 intragastrically) group,and selegiline(0.5 mg?kg-1 intraperitoneally) group.After pretreatment with drugs for 10 days,the latter 3 groups were given 1-methyl-4-phenyl-1,2,3,6-tetrahyrdropyridine(MPTP) intraperitoneally to establish Parkinson's disease(PD) model,then the mice were put to death for the determination of the activities of MAO-B,SOD and GSH-px in blood serum and brain tissue.RESULTS:As compared with blank control group,the activity of MAO-B in model group increased slightly,while the activities of SOD and GSH-px decreased(P

Objective To carry out investigation and analysis in present situation of vocational ethnics of clinical nursing teachers in order to supply reference for quality improvement of nursing staff. Methods Inter-views were carried out in 40 clinical nursing teachers in 3 teaching hospitals, Meanwhile, 700 nursing stu-dents who were in practice and probation were investigated by questionnaires about vocational ethnics of clinical nursing teachers. Results 91.5% students thought that clinical nursing teachers were uncorrupt-ed and self- disciplined, 93.0% of them considered that they could hold their ground. Most students gave positive evaluation about the teaching attitude, teaching ability and teaching practice. Conclusions We should further perfect the vocational ethnics criterion, improve cultivation system, strengthen the moral edu-cation in order to promote the vocational ethnics construction.

Tetanus is a potentially fatal but largely preventable disease through immunisation.Despite efforts by the World Health Organization to eradicate the disease,tetanus(particularly severe tetanus)remains a worldwide cause of morbidity and mortality.Timely diagnosis and appropriate intensive care management are essential to ensure survival.Recently,with progress in the treatment of severe tetanus,the mortality has been decreased significantly.