BACKGROUND: At present, more and more people pay attention to the protection of sub-hypothermia on cerebral ischemic tissue and the effect of sub-hypothermia treatment on inflammatory reaction after cerebral ischemia-reperfusion.OBJECTIVE: To investigate the effect of sub-hypothermia on inflammatory cytokine in cerebral ischemic area of rats and protection on cerebral reperfusion injury.DESIGN: Randomized controlled study on the basis of animals.SETTLNG: People's Hospital of Hubei Province.MATERIALS: The experiment was completed at Laboratory Center of Renmin Hospital of Wuhan University from October 2004 to February 2005. Totally 50 healthy SD rats of clean grade were selected in this study.METHODS: Ten SD rats were divided randomly into normal group and sham operation group with 5 in each group. Other 40 rats were divided randomly into normal temperature cerebral ischemia group and sub-hypothermia cerebral ischemia group with 20 in each group. Rats except 5 in normal group were used to establish reversible ligation model of left middle cerebral artery (MCA) with Longa ligation method. Rats in sham operation group and normal temperature group were put in 20 ℃ room, and anus temperature was maintained at 37 ℃; rats in sub-hypothermia group were put in 4 ℃ room and anus temperature was maintained at (33.0±1.0) ℃.Rats in sub-hypothermia group were treated with sub-hypothermia on whole body, after ischemia, temperature was changed normally, and reperfusion was started 3 hours after cerebral ischemia. All modeling rats were scored with neurological defect: 0 point: none of neurological defect; 1 point: unable to unfold bilateral anterior claws; 2 points: cycling to hemiplegia side during walking; 3 points: falling to hemiplegia side; 4 points: unable to self-walk and with unconsciousness.MAIN OUTCOME MEASURES: Expression of tumor necrosis factor (TNF) andinterleukin-1 in cerebral tissue of ischemic rats, percentage of volume of cerebral infarction,and neurological functional scores.RESULTS: Fifteen rats were excluded because of intracranial hemorrhage,anesthetic accident and unqualified neurological functional scores. Other 15 rats with successful modeling were supplied randomly, and totally 50rats entered the final analysis. ① Number of TNF positive cell in immunohistochemical staining: There were no significant differences between normal group and sham operation group [(3.54±1.24, 3.71±1.50) /sight]; but numbers in sub-hypothermia group were lower than those in normal temperature group [(31.94±7.23, 69.20±9.43)/sight, F=179.16, P ＜ 0.001]. ②Numbers of interleukin-1 positive cells in immunohistochemical staining:There were not significant differences between normal group and sham operation group [(3.20±1.34, 3.89±2.08) /sight]; but numbers in sub-hypothermia group were lower than those in normal temperature group [(28.95±4.97, 55.79±7.93)/sight, F=174.95, P ＜ 0.001]. ③ Volume percentage of infarct focus: Percentage in sub-hypothermia group was lower than that in normal temperature group [(21.06±2.42)%, 30.32±2.71], F =374.87, P ＜ 0.001]. ④ Neurological functional scores: Percentage in subhypothermia group was lower than that in normal temperature group [(1.35±0.27)%, (2.04±0.34)%, F=117.17, P ＜ 0.001].CONCLUSION: ① Volume of cerebral infarction focus is lower in subhypothermia group than that in normal temperature group, this suggests that sub-hypothermia can protect ischemic neurons. ② Positive expressions of TNF and interleukin-1 are observed rarely in normal group and sham operation group, but more expression of positive cells are observed in cerebral ischemic area with 24-hour reperfusion after ischemia, this suggests that cerebral ischemia starts expression of inflammatory cytokine, evoke inflammatory cascade reaction, deteriorate cerebral ischemia-reperfusion injury. Therefore, inhibition of inflammatory cascade reaction is one of important mechanisms of protecting brain.

In recent years, many versions of international standard terminologies on TCM have been established and enacted by different authoritative organizations worldwide. However, infantile tuina acupoints have never been included in them. The article illustrated the necessity of including infantile tuina acupoints in the international standard terminologies on TCM from the aspects of history, present state and specificity of these points. It also pointed out some problems of infantile tuina acupoints, such as different records of acupoints numbers, applications in the clinic and strategies applied in their English translation, and suggestions were provided accordingly, hoping that the infantile tuina acupoints can be included in the further revised versions of international standard terminologies on TCM.

Allograft rejection after xero organ transplantation rejection, especially acute rejection is still the major reason of failure and death. Active T cell play key roles in allograft rejection. It has been showed that the expressions of costimulatory molecules are associated with xero organ transplantation rejection. The pathways of CD28/CTLA-4 and CD40/CD40L are important costimulatory pathways that cause T cell activation.The article emphasizes on the role of CD28/CTLA-4-B7 pathway in allograft rejection.

Objective To observe the effect of asarone injection on terms of airway inflammation factor in asthmatic children.(Metho)ds Sixty asthmatic children were chosen and divided randomly into methotrexate group and control group.In the methotrexate group,intravenous injection with 2 times per day was given.The concentration of the injection solution was 0.01%,which was mixed by(0.5 mg/kg) asarone injection and 10% glucose injection.In control group,intravenous injection once per day was given.The solution consisted of 4-6 mg/kg aminophyline injection and 100 mL 5% glucose injection.Every 7 continuous days was taken as one process stage.Two process stages were observed during the experiments.Results After two weeks,the eosinophils and the macrophage were changed significantly(P

Cysts ; Humans ; Laryngeal Diseases ; Male ; Middle Aged

Objective To investigate the positive end-expiratory pressure (PEEP) ventilation in the treatment of neurogenic pulmonary edema. Method 64 patients with neurogenic pulmonary edema were treated with the timely use of positive end-expiratory pressure(PEEP) ventilation. Then the curative effect was observed. Result The symptoms of hypoxia, pulmonary edema were had a rapid improvement in ventilation with PEEP. the improvementof heart rate, respiratory rate, oxygen saturation, partial pressure of oxygen, carbon dioxide partial pressure had significant difference compared with that before treatment(P ＜ 0.01). Conclusion Neurogenic pulmonary edema patients treated with PEEP therapy had goved clinical effect.

Objective To explore the effect of music therapy on post-stroke depression (PSD) with left basal ganglia aphasia. Methods From April, 2013 to July, 2016, 60 patients with PSD and left basal ganglia aphasia were selected as control group (n=30) and research group (n=30). Both groups accepted routine medicine and rehabilitation, and the research group accepted music therapy in addition, for three months. They were assessed with modified Stroke Aphasic Depression Questionnaire Hospital Version (SADQ-H) and China Rehabilitation Research Center Aphasia Examination (CRRCAE) before and after treatment. Results The incidence of serious depression was less in the re-search group than in the control group after treatment, according to the score of SADQ-H (χ2=8.926, P<0.05). The research group improved more in listening comprehension, repetition and name scores than those of the control group (t>5.707, P<0.001). Conclusion Music therapy helps to relief depression and improve speech in patients with PSD and basal ganglia aphasia.

Objective To explore the effects of health education combined with WeChat′s public platform in the management of risk factors in patients with coronary heart disease. Methods A total of 100 coronary heart disease patients were randomly divided into intervention group and control group and there were 50 cases in each group by the random number table. The intervention group received health education about risk factors combined with WeChat′s public platform for 6 months. The control group received routine health education. Evaluate the control rate of blood pressure, blood glucose and blood lipid and the score of coronary heart disease self-management scale of the two groups before and after the intervention. Results At the 6th month after intervention, the control rate of blood pressure of the intervention group was 88.0％(44/50) and it of the case group was 70.0％(35/50).The difference between the 2 groups was statistically significant (χ2=4.882, P < 0.05). The score of coronary heart disease self-management scale of the intervention group was (102.44±8.22) points and it of the case group was (89.82±8.01) points . The difference between the 2 groups was statistically significant(t=7.776, P < 0.05). Conclusions The health education combined with WeChat′ s public platform contributed to help coronary heart disease patients to establish good self-management behavior and it can be widely used in the management of coronary heart disease risk factors.

Objective To study the efficacy of fixation with knee arthroscopy as adjuvant therapy for lateral tibial plateau fracture. Methods 72 cases with lateral tibial plateau fracture of our hospital from September 2011 to September 2014 were divided into experimental group (36 cases) and control group (36 cases), according to different operation method, the experimental group using arthroscopic, while the control group using traditional incision surgery. Then the incision length, operative time, hospital stay, wound healing, bone healing time were compared. Complications, knee function and reduction of the fracture were compared. Results The incision length of experimental group was significantly lower than the control group, the difference was statistically significant (P <0.05). Operative time, hospital stay, wound healing time, the bone healing time were significantly lower than the control group, the differences were statistically significant (P < 0.05). Delay healing, joint stiffness and traumatic arthritis were significantly lower than the control group, the differences were statistically significant (P < 0.05). RASMUSSEN function score and radiographic scores in the experimental group were significantly higher than the control group, the differences were statistically significant (P < 0.05). Conclusion Fixation with knee arthroscopy as adjuvant therapy for lateral tibial plateau fracture has advantages of smaller incision, shorter operative time and hospital stay and healing time, lower complication rate while better reduction of the fracture and function than conventional methods.