This article discussed the cultivation of Double Qualified Teacher for prosthetics and orthopedics education.

Objective To investigate the value of combined detection of GGT,blood lipid and hs-CRP in patients with coronary heart disease.Methods Totally 134 patients with coronary heart disease and 38 patients without coronary heart disease were selected after coronary angiography.Coronary heart disease patients were divided into mild stenosis group (group A,n =40),Moderate stenosis group (group B,n =38) and severe stenosis group (group C,n =56) according to Gensini score.The age,sex,GGT,blood lipid and hs-CRP levels were detected in all groups,and t-he statistical analysis was performed by using SPSS 22.0 software.Results The level of GGT,TC,TG,LDL and hs-CRP in CHD group were all higher than those in non CHD group (P ＜ 0.05).The level of HDL was lower than that in the non CHD group (P ＜ 0.05).The level of GGT,TC,TG,LDL,hs-CRP in group B and C were higher than group A (P ＜0.05).The level of HDL in group B and C were lower than group A(P ＜0.05).The level of GGT,TC,TG,LDL,hs-CRP in group C were higher than group B (P ＜ 0.05).Logistic regression analysis showed that serum GGT,TG,hs-CRP were independent risk factors for coronary heart disease (P ＜ 0.05),HDL was an independent protective factor for coronary heart disease (P ＜ 0.05).Conclusion The combined detection of GGT,blood lipid and hs-CRP has important value in the diagnosis of coronary heart disease.

Diabetes mellitus is a common disease of serious harmful to human health,but its pathogenesis is not entirely clear.Mitochondria are the important organelles to generate energy in eukaryocytes,and play a pivotal role in the regulation of reactive oxygen species generation,intracellular calcium homeostasis,and apoptosis signal transduction.The possible causes of mitochondrial dysfunction include oxidative stress,Ca2+ disturbances,reduction of mitochondrial biosynthesis,opening of mitochondrial permeability transition pore,mitochondrial DNA mutations,and etc..Many studies demonstrate that mitochondrial dysfunction is associated with β-cell dysfunction of type 1 diabetes mellitus and insulin resistance of type 2 diabetes mellitus.Moreover,mitochondrial dysfunction plays important roles in the development of diabetic cardiomyopathy.This present article reviewed the current status of studies on the relationship of mitochondrial dysfunction and diabetes mellitus and diabetic cardiomyopathy.It is very important to understand and study mitochondrial dysfunction and its important roles in diabetes mellitus and diabetic cardiomyopathy in order to clarify the pathogenesis of diabetes mellitus and explore new approaches of prevention and treatment for diabetes mellitus.

Spermatogenesis is a complex process. Current knowledge about human spermatogenesis is mainly based on the mouse model while little is known about the initial stage of this fundamental process in humans. The establishment of the model of spermatogenesis in vitro may contribute to an overall understanding of male germ cell development, an insight into the mechanisms of infertility, and clinical management of male infertility. This review summarizes current knowledge about the generation of germ cell-like cells from induced pluripotent stem cells (iPSCs) in vitro and discusses the potential application of iPSCs in the treatment of male infertility.
Animals ; Cell Differentiation ; Germ Cells ; Humans ; Induced Pluripotent Stem Cells ; Infertility, Male ; therapy ; Male ; Mice ; Spermatogenesis

Objective To proceed deep data mining in large database obtained by Cancer Genome Anatomy Project and to distinguish the difference expression genes of gastric carcinoma.Methods The 300,783 serial analysis of gene expression records from 4 libraries of gastric cancer and 2 libraries of normal gastric tissue in database of the Cancer Genome Anatomy Project were analyzed by digital gene expression displayer.Results The 201 difference expression tags represented 136 genes(54 up-regulated and 82 down-regulated) and 65 ESTs(24 up-regulated and 41 down-regulated) were distinguished,and these genes were involved in biological process and signal transduction pathways,such as cell cycle regulation,cell proliferation and apoptosis.The gene expression profile in whole body normal and cancer tissues of PTMA were constructed by digital Northern based on serial analysis of gene expression database and by virtual Northern based on cDNA database,respectively.Conclusion The difference expression genes of gastric cancer can be distinguished effectively with bioinformatics,and the results will guide our further molecular biology research.If validated by molecular biology experiment,the difference expression genes will be used as molecular targets of gastric carcinoma.

Objective:To investigate the effect of Ginkgo leaf extract and dipyridamole injection on serum tumor necrosis factor-α, procalcitonin, lipopolysaccharide and 28-day mortality in patients with sepsis.Methods:Ninety patients with sepsis who received treatment in Ningbo Yinzhou People's Hospital from February 2018 to February 2019 were included in this study. They were randomly assigned to receive routine treatment (control group, n = 45) or Ginkgo leaf extract and dipyridamole injection treatment combined with routine treatment (treatment group, n = 45). Clinical efficacy, preoperative and postoperative Acute Physiology and Chronic Health Evaluation II score, Sequential Organ Failure Assessment score, serum tumor necrosis factor-α, procalcitonin, bacterial lipopolysaccharide levels as well as 28-day mortality were compared between the control and treatment groups. Results:There was significant difference in total effective rate between the treatment and control groups [91.11% (41/45) vs. 75.56% (34/45), χ 2 = 6.690, P = 0.039]. After treatment, Acute Physiology and Chronic Health Evaluation II score and Sequential Organ Failure Assessment score in the treatment group were (15.93 ± 1.01) points and (6.25 ± 1.83) points, respectively, which were significantly lower than those in the control group [(18.62 ± 1.75) points, (8.54 ± 2.19) points, t = 8.931, 5.383, all P < 0.001]. After treatment, serum tumor necrosis factor-α, procalcitonin and lipopolysaccharide levels in the treatment group were (43.62 ± 3.39) ng/L, (1.46 ± 0.79) μg/L, (23.62 ± 7.19) ng/L, respectively, which were significantly lower than those in the control group [(56.28 ± 4.54) ng/L, (2.12 ± 1.03) ng/L, (33.75 ± 8.67) ng/L, t = 14.989, 3.411, 6.033, P < 0.001, P = 0.001, P < 0.001]. The 28-day mortality in the treatment group was significantly lower than that in the control group [6.67%(3/45) vs. 22.22%(10/45), χ 2 = 8.160, P = 0.017]. Conclusion:Based on routine treatment, Ginkgo leaf extract and dipyridamole injection can improve the clinical symptoms of patients with sepsis, reduce inflammatory reaction and decrease mortality.

Objective To discuss the clinical applied value of MSCT MPR in displaying the relation between periampullary diverticula(PAD) and pancreaticobiliary duct.Methods There were 32 patients with PAD found through the volume scans by MSCT, and proved by endosope or GI. The axial source images after being reconstructed were transmitted to Vitrea workstation for observation, and the images which were valid to show the relation between PAD and pancreaticobiliary duct were get.Results In 32 patient,34 PADs were found,22PADs located above the papilla , 7 located in the neck edge of diverticula , 4 were papilla in diverticula and 1 located below papilla .Conclusion MPR can clearly display PAD and pancreaticobiliary duct, their relation and pancreaticobiliary disease.

Objective To search for clinical appearance and fungal resistance of candidemia in ICU.Methods Thirty-four cases of candidemia in ICU during 2003and 2006 were analyzed retrospectively.Results Twelve of 34 cases of candidemia were C.albicans,and 22 of 34 cases were non-C.albicans,which were 64.71% in candida monilia.Fever and chill of candidemia were 100% and 70.59% differently.Conclusion If there is high risk factor of fungous infection in clinic,at the same time fever and chill appear,and antibiotics treat is inefficacy not effective,it is possible to consider candidemia.

Objective To compare the effect of electroacupuncture at head points and body points on glucose metabolism of the cerebral motor function regions in normal subjects.Methods To observe the change of glucose metabolism of cerebral motor area in normal subjects between before and after acupuncture during the movement by PET.Talairach coordinates(Atlas of brain) and statistical parametric mapping(SPM) software were used to deal with the acquired imaging data.Results ① Acupuncture at Baihui(GV20) and left Qubin(GB7) could increase metabolism of glucose in bilateral Lps and precuneus,the activation of left area in the brain being more significant.②Acupuncture at right Quchi(LI11) and Zusanli(ST36) change metabolism of glucose in left gyrus precentralis,right loblus paracentralis,right gyrus frontalis medialis,both cerebellums and both putarnens.Conclusion All acupoints can change glucose metabolism in cerebral structures related to motor function in the bilateral cerebral hemispheres,different acupoints active different motor areas.The function of acupuncture is a kind of comprehensive regulative process.