Child ; Herpesviridae ; classification ; Herpesviridae Infections ; diagnosis ; Humans ; Paramyxoviridae ; classification ; Paramyxoviridae Infections ; diagnosis ; Parechovirus ; classification ; Pediatrics ; Picornaviridae ; classification ; Picornaviridae Infections ; diagnosis ; SARS Virus ; classification ; Severe Acute Respiratory Syndrome ; diagnosis ; Virus Diseases ; diagnosis ; Viruses ; classification

Objective To examine whether electroacupuncture at the Wangu acupoint (GB 12 ) , whose position is similar to the cerebellar fastigial nucleus ,can reduce inflammatory cytokines in the hippocampus of rats with vascular dementia (VD) to provide theoretical evidence for treating VD with electroacupuncture .Methods Healthy Sprague‐Dawley rats ( n=54 ,300 -450 g) were randomly divided into three groups :sham surgery group ,VD group ,and electroacupuncture group .The ethologic scores of VD rats were evaluated and the mRNA expressions of inflammatory cytokines (TNF‐α,IL‐6 and IL‐1β) in the hippocampus were assessed and the hippocampal tissues were observed by hematoxylin‐eosin (HE) staining .Results Compared with VD group ,in electroacupuncture group the rats' learning ability improved significantly and the mRNA expressions of TNF‐α, IL‐6 and IL‐1β decreased . Simultaneously ,the damage extent of nerve cells in the hippocampal tissues decreased , and their morphology recovered to nearly normal .Conclusion Electroacupuncture at the Wangu acupoint can decrease the level of inflammatory cytokines in the hippocampus and reduce the damage extent of nerve cells in the hippocampus ,thus providing a new neuroprotective method for VD .

Objective:To study the significance of QT dispersion(QTd) changes in electrocardiogram(ECG) after coronary artery bypass graft. Methods:Forty eight patients scheduled for elective coronary artery bypass graft were studied. The changes of QTd were monitored by 12 lead electrocardiogram system before surgery (as baseline values), at the end of surgery, and 1 4 d and 7 d after surgery.Results:The baseline values for QTd was (56.32?2.25) ms.QTd were significantly longer as compared to the baseline values ( P

Galanin is a peptide with wide-ranging effects,especially within the central,peripheral nervous system and endocrine system.Many tumors of neuroendocrine origin,and also an increasing number of non-neuroendocrine cancers,have been shown to express galanin and/or its receptors.Expression of galanin peptide has been detected in pheochromocytoma,squamous cell carcinoma,pituitary adenoma,gastrointestinal cancers and so on.Galanin peptide plays an important role in tumori-genesis and progression of many kinds of tumors.Therefore,studying the roles of galanin and its receptors are helpful for diagnosis and treatment of tumors.

Background Stickler syndrome is a genetic connective tissue disorder that affects the ocular,skeletal,orofacial and auditory systems.To determine the gene mutation loci can offer a basis for genetic diagnosis and management of Stickler syndrome.Objective The aim of this study was to research the clinical characteristics of a pedigree with Stickler syndrome and identify the disease-causing gene mutation.Methods This study was approved by Ethic Committee of Peking Union Medical College Hospital.The clinical study and pedigree analysis were performed in one family with Stickler syndrome type Ⅰ (STL Ⅰ).Nine family members were examined with informed consent.The entire coding regions of COL2A1 gene with flanking intronic regions were amplified by PCR and directly sequenced.The detected sequence change was confirmed to be mutationloci by examining whether they existed in normal control individuals.Mutant proteins were predicted with online software.Results There were 4 generations and 11 members in this family,and 2 members died,including 1 patient.Three patients were found in 9living families.Inheritance of this family complicd with an autosomal dominant inheritance mode.All affected individuals showed the consistent phenotypes with STL Ⅰ,including high myopia,membranous vitreous anomaly and surface central flat,short nose,palatoschisis,etc.Mutation screening of COL2A1 gene revealed that the first base of intron 12 was deleted(IVS12+1G del).Nucleotide sequence analysis showed that this mutation led to the functional abnormal of this gene by forming termination cordon in advance.This mutation occurred in all affected individuals,however,no mutation was observed in any unaffected member or 100 normal unrelated individuals.Conclusions This study identifies a novel splice-site mutation(IVS12+ 1G del)in COL2A1 gene in a Chinese STL Ⅰ pedigree.This is the first report on a mutation in a Chinese STL Ⅰ family.

Objective:To discuss the effect of individualized physical exercise prescription on patients’ nutrition status, physical ability and self-care ability in patients with intestinal fistula and severe malnutrition. Methods:Twenty-four patients were divided randomly into two groups, the experiment group exercised according to physical exercise prescription, control group exercised in a routine way. The patients’ weight, physical ability and self-care ability were measured on the day before exercise and the 28th day after exercise. Results:Patients in the exercise group had significant more exercise than those in the routine group, and the physical ability and self-care ability were significant better that those in the routine group. Conclusion:Individualized physical exercise prescription can accelerate the recovery of physical ability and self-care ability, it can be used safely and effectively in patients with intestinal fistula and severe malnutrition.

Objective To investigate the level of serum CA125 in patients with non-Hodgkin's lymphoma(NHL) and its clinical value.Methods All the data of this study was collected from the first affiliated hospital of China Medical University,during September 2004 to March 2006.Serum CA125 levels were measured in 42 patients with NHL.The association with gender,clinical stages,B symptoms,effusions,International Prognostic Score(IPI),serum LDH(lactate dehydrogenase),beta 2 microglobulin(?2-MG) levels and response to treatment was evaluated.Results High level of CA125 was found to be obviously correlated with B symptoms,effusions,IPI score,serum LDH(P

AIM:To study the pharmacological action of Zhikang Capsule (Herba et Radix, Paederiae scandentis, etc.) METHODS: To establish the model of anus injury in the rectal position on rabbit, anti-inflammation and analgesia effect in rats and mice were observed by carrageen and hot-plate method, and inflammatory mediator PGE_2 of rat paw edema was analysed. RESULTS: Zhikang Capsule had the obvious effect to help heal and recover the anus injury in the rectal position and it could inhibit rats’ thorax leukocytoplania and rat paw edema of Garrageenan-induced and cotton pellet-induced granuloma formation in mice. The exudation of blood capillaryies and the contents of inflammatory medium PGE_2 were lowered and improved in the pain threshold. CONCLUSION: Zhikang Capsule has the obvious effect on the healing and recovery of the anus injury in the rectal position, its anti-inflammiting effect is related to the decrease in the exudation of blood capillaries and the contents of inflammatory medium PGE_2.

Objective To invstigate the effect of high dose X-ray irradiation on the expression of multidrug resistance-1 (MDR1), Bcl-2, MMP7 genes. Methods A nasopharyngeal carcinoma cell line,CNE1, were irradiated with a total dose of 50 Gy. The resistance to the cisplatin of CNE1 cells and the irradiated CNE1 (CNE1 R) cells was detected by MTT. mRNAs expression of MDR1 , Bcl-2 and MMP7 was measured by quantitative RT-PCR. Results The expression of MDR1 increased in CNE1 R cells. The semiquantitative A value of MDR1 mRNA was 0.47 ±0.04, and the value of CNE1R cells (1st, 7th, 21st,28th, 35th, 42nd and 49th days after irradiation) were 0.67 ± 0. 06 (t = -5.44, P = 0. 003) ,0.70 ± 0. 01(t=-5.90,P=0. 002),0.73±0. 01(t= -6. 45,P=0. 001) ,0. 67 ± 0. 03 (t= -3.97,P=0.011),0.65 ±0.01(t = -4.43,P=0. 007),0. 62±0. 05(t= -2. 64,P=0.046) and 0.62 ±0.02(t = -3.34,P=0.021), respectively. Bcl-2 mRNA expression were 0.55 ±0.02 and 1.05 ±0.04(t = -9.93,P=0. 000) and MMP7 mRNA expression were 0.51 ±0.01 and 0.82 ±0.02(t = -8.51,P=0.000) in CNE1and CNE1 R cells. Conclusions The MDR1 expression was increased after a total dose of50 Gy irradiation,which may be related to the synchronous change of Bcl-2 and MMP7 genes.

Objective To observe the clinical efficacy of heat-clearing, dampness-removing, and Qi-blood-tonifying Chinese herbal medicine compound for the treatment of recurrent genital herpes(RGH), and to investigate the expression levels of Th17 cells and Treg cells in peripheral blood of patients with RGH as well as their role in the pathogenesis of RGH. Methods Sixty RGH patients were randomly divided into experimental group and control group, 30 cases in each group. The experimental group was treated with Chinese medicine decoction, and the control group was treated with Aciclovir tablets orally. The treatment course covered 6 months. The percentages of Th17 cells and Treg cells in the peripheral blood of the RGH patients were detected with flow cytometry. Results (1) After treatment for 3, 6 months, the total effective rate of the experimental group was 53.33%, 80.00%, and that of the control group was 23.33%, 46.67% respectively. There were significant difference between the two groups(P < 0.05).(2) After treatment, the percentage of Th17 cells and the ratio ofTh17/Treg cells were decreased while the percentage of Treg cells was increased in the two groups, and the differences before and after treatment were significant in the experimental group(P<0.05) while were insignificant in the control group (P > 0.05). The inter-group comparison showed the decrease of Th17 cell percentage and Th17/Treg cell ratio as well as the increase of Treg cell percentage in the experimental group was superior to that in the control group, the differences being significant (P<0.05). Conclusion The curative effect of Chinese herbal compound for RGH is better than that of the control group. Chinese herbal medicine compound can decrease the level of Th17 cells, increase the level of Treg cells, thus to decrease the ratio of Th17/Treg cells, indicating that heat-clearing, dampness-removing, and Qi-blood-tonifying Chinese herbal medicine compound can correct the imbalance of Th17/Treg cells, and then plays a role in anti-inflammation and immune regulation.