Adaptation, Psychological ; Adolescent ; Adult ; Coal Mining ; Humans ; Male ; Mental Health ; Middle Aged ; Psychology, Industrial ; Young Adult

Objective To explore the relationship between mechanical withdrawal threshold ( MWTs) and local cutaneous blood perfusion ( BP ) in rats with acute inflammation induced by carrageenan.Methods Twenty male Sprague-Dawley ( SD) rats were randomly divided into control and model groups.The acute inflammatory rat model was established by subcutaneously injecting with carrageenan into the left hindpaw.MWTs were measured by Dynamic Plantar Aesthesiometer 37450 before injection ( as base) of carrageenan and at 4 h, 24 h, 48 h, 72 h after carrageenan injection. The local cutaneous BP was detected by Pericam Perfusion Speckle Imager ( Pericam PSI) at the time after measuring of MWTs.The above two behaviors were compared and the relationship between them was analyzed.Results MWTs of the model group rats were decreased while BP significantly increased than that in the control group ( P<0.01 ) .The MWTs and BP in the model rats showed a negative correlation, especially the correlation index showed significant differences at 4 h and 72 h after carrageenan injection ( P<0.01) .Conclusions Carrageenan-induced acute inflammation in rats causes significant changes in mechanical pain threshold, which has a negative correlation with local cutaneous blood perfusion.

Objective To observe the distribution features of phosphorylated p 44/42 extracellular signal-regulated kinase(p-ERK) induced by affective pain in the anterior cingulate cortex (ACC) of the rat.Methods Twelve male SD rats were randomly divided into the control group (n=6) and spinal nerve ligation (SNL) group (n=6).The rats of the SNL group were exposed to unilateral L5 spinal nerve ligation (SNL) surgery.The behavior was examined by mechanical pain thresholds (MPTs) and the pain affect was measured using open-field test, elevated zero maze.The expressions of p-ERK in ACC (AP 3.2,2.7,2.2mm from Bregma) were detected with immunofluorescence test .Results In comparison with the control group , the unilateral mechanical pain threshold in the SNL group decreased significantly after operation . The operation also resulted in pronounced anxiety-like behavior .The p-ERK labelled cells in different levels of ACC ( AP 3.2,2.7,2.2mm from Bregma) of SNL rats were (11.89 ±2.57),(32 ±4.67 ) and (17.56 ±2.04 ), respectively, while the control group rats were (12.44 ±2.16),(10 ±0.87 ) and (10.11 ±1.36).These data indicated that compared with the control group, the expressions of p-ERK in ACC(AP 2.7,2.2mm from Bregma) increased significantly in the SNL group after operation(P<0.01), besides for the level of ACC(AP 3.2mm from Bregma) (P>0.05).Conclusion The results suggest that the neuropathic pain can result in anxiety-like behavior and increased expressions of p-ERK in ACC of the rat.This change may be closely related to the expressions of p-ERK in ACC(AP 2.7,2.2mm from Bregma), but not to the level of ACC(AP 3.2mm from Bregma).

0.05);the elongation (%) 470?9.5,560?8.2 and 470?5.8 (SY-1 versus SY-2 or MDX-4-4210,P

0.999) and the limits of detection were 0.03-0.6 ng/ml. The average recoveries were 85.5%-113.1%, RSDs were 1.8%-4.6%. Conclusion With the accuracy, high sensitivity and reproducibility, the method is suitable for the determination of organochlorine pesticide residues in the honey.

Workplace

Objective To observe the clinical efficacy of acupuncture plus auricular point sticking in improving tobacco withdrawal symptoms after smoke cessation. Method Forty-eight patients with tobacco withdrawal symptoms were randomized into a treatment group and a control group, 24 in each group. The treatment group was intervened by acupuncture plus auricular point sticking, while the control group was by auricular point sticking. The daily consumption of tobacco, Fagerstrom Test of Nicotine Dependence (FTND), and Self-rating Scale of Tobacco Dependence (SSTD) were observed before and after intervention, and the smoke cessation rate was also compared. Result The smoke cessation rate was 83.3%in the treatment group versus 54.2%in the control group, and the difference was statistically significant (P<0.05). After intervention, the daily consumption of tobacco and SSTD score in the treatment group were significantly changed and markedly different from that in the control group (P<0.05). The FTND scores were significantly changed after intervention in both groups (P<0.05). There was a significant difference in comparing the FTND score between the two groups after intervention (P<0.05). Conclusion Acupuncture plus auricular point sticking can improve the tobacco withdrawal symptoms.

Objective To observe the clinical efficacy of needling the Chorea Trembling Control Area in treating Parkinson’s Disease (PD). Methods Sixty patients with PD were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by needling the Chorea Trembling Control Area plus oral administration of Levodopa and Benserazide Hydrochloride;while the control group was by oral administration of Levodopa and Benserazide Hydrochloride alone. The clinical efficacies were compared after 3 treatment courses. Results After intervention, the Webster scores were significantly changed in both groups (P<0.05). There was a significant difference in comparing Webster score between the two groups after intervention (P<0.05). The total effective rate was 93.0%in the treatment group versus 83.0%in the control group, and the difference was statistically significant (P<0.05). Conclusions Acupuncture at the Chorea Trembling Control Area plus medi-cation is an effective method in treating PD.

Objective: To discuss the influence of comprehensive nursing intervention on patient’s oral mucositis with hematopoietic stem cell transplantation. Methods:One hundred and seventy four examples of patients with hematopoietic stem cell transplantation were equally and randomly divided into two groups, which are intervention group and control group. The usual care was given to the control group, while both the usual care and comprehensive nursing intervention were given to the intervention group. The comparative study of the occurrence and severity degree of oral mucositis in these two groups after one week pretreatment was conducted. Results:The occurrence rate of oral mucositis in intervention group and control group was 54.47%and 68.97%respectively. And there is no obvious significance of the occurrence rate before and after the intervention(x2=2.47, P>0.05). Nevertheless, the comprehensive nursing intervention could significantly lessen the severity of oral mucositis, and help to control the inflammation and promote healing. Conclusion:comprehensive nursing intervention could effectively reduce the occurrence degree of oral mucositis in the patients with hematopoietic stem cell transplantation.

Objective To investigate the changes of serum myocardial enzyme in schizophrenia patients spectrum and its clinical significance.Methods 405 patients with schizophrenia hospitalized patients serum myocar-dial enzyme (CK,CK-MB,LDH,α-HBDH level,) on admission and 4 weeks after treatment the serum myocardial enzyme level,analyze its relationship with the disease, and 100 healthy people ( control group) were compared. Results the positive symptoms of schizophrenia patients before treatment,serum CK,CK-MB,LDH,α-HBDH were (1 286.52 ±714.38) U/L,(40.72 ±27.38) U/L,(143.06 ±33.24) U/L,(115.75 ±22.74) U/L,CK,CK-MB were higher than those in the control group (t=10.35,9.29,all P<0.01);after 4 weeks of treatment,CK,CK-MB, LDH,α-HBDH were (98.13 ±38.75)U/L,(12.87 ±5.73)U/L,(125.43 ±25.74)U/L,(102.15 ±26.86)U/L, compared with the control group,the differences were not statistically significant(all P>0.05).Conclusion Serum myocardial enzyme CK and CK-MB increased level was related with diseasein the patients with acute exacerbation of schizophrenia.