Adaptation, Psychological ; Adolescent ; Adult ; Coal Mining ; Humans ; Male ; Mental Health ; Middle Aged ; Psychology, Industrial ; Young Adult

Objective To observe the distribution features of phosphorylated p 44/42 extracellular signal-regulated kinase(p-ERK) induced by affective pain in the anterior cingulate cortex (ACC) of the rat.Methods Twelve male SD rats were randomly divided into the control group (n=6) and spinal nerve ligation (SNL) group (n=6).The rats of the SNL group were exposed to unilateral L5 spinal nerve ligation (SNL) surgery.The behavior was examined by mechanical pain thresholds (MPTs) and the pain affect was measured using open-field test, elevated zero maze.The expressions of p-ERK in ACC (AP 3.2,2.7,2.2mm from Bregma) were detected with immunofluorescence test .Results In comparison with the control group , the unilateral mechanical pain threshold in the SNL group decreased significantly after operation . The operation also resulted in pronounced anxiety-like behavior .The p-ERK labelled cells in different levels of ACC ( AP 3.2,2.7,2.2mm from Bregma) of SNL rats were (11.89 ±2.57),(32 ±4.67 ) and (17.56 ±2.04 ), respectively, while the control group rats were (12.44 ±2.16),(10 ±0.87 ) and (10.11 ±1.36).These data indicated that compared with the control group, the expressions of p-ERK in ACC(AP 2.7,2.2mm from Bregma) increased significantly in the SNL group after operation(P<0.01), besides for the level of ACC(AP 3.2mm from Bregma) (P>0.05).Conclusion The results suggest that the neuropathic pain can result in anxiety-like behavior and increased expressions of p-ERK in ACC of the rat.This change may be closely related to the expressions of p-ERK in ACC(AP 2.7,2.2mm from Bregma), but not to the level of ACC(AP 3.2mm from Bregma).

Objective To explore the relationship between mechanical withdrawal threshold ( MWTs) and local cutaneous blood perfusion ( BP ) in rats with acute inflammation induced by carrageenan.Methods Twenty male Sprague-Dawley ( SD) rats were randomly divided into control and model groups.The acute inflammatory rat model was established by subcutaneously injecting with carrageenan into the left hindpaw.MWTs were measured by Dynamic Plantar Aesthesiometer 37450 before injection ( as base) of carrageenan and at 4 h, 24 h, 48 h, 72 h after carrageenan injection. The local cutaneous BP was detected by Pericam Perfusion Speckle Imager ( Pericam PSI) at the time after measuring of MWTs.The above two behaviors were compared and the relationship between them was analyzed.Results MWTs of the model group rats were decreased while BP significantly increased than that in the control group ( P<0.01 ) .The MWTs and BP in the model rats showed a negative correlation, especially the correlation index showed significant differences at 4 h and 72 h after carrageenan injection ( P<0.01) .Conclusions Carrageenan-induced acute inflammation in rats causes significant changes in mechanical pain threshold, which has a negative correlation with local cutaneous blood perfusion.

[Objective]To observe the intervention of Electroacupuncture at Point Jianyu(LI15) on Rabbit Model of Scapulohumeral Periarthritis for receiving possible mechanism.[Methods ]Thirty healthy rabbits(male or female) were randomly divided into three groups:scapulohumeral periarthritis model group,EA Jianyu group and Ibuprofen control group.Each group respectively were detected the concentrations of serum PGE2 before model and treatment and after treatment,and then killed them to achieve tendons of shoulder which were detected the concentrations of Hydroxyproline.And achieve tissues around shoulder which were studied by HE to investigate the changes of the histology.[Results]The concentrations of serum PGE2 had no notable difference between EA Jianyu and model group,Ibuprofen control group and model group after treatment.But it was lowered after treatment than before treatment by EA Jianyu.There were no significantly difference among the three groups about concentrations of Hydroxyproline in tendons,but Ibuprofen control group was higher than EA Jianyu group.Tissues of the shoulder were observed that EA Jianyu restrained reaction of inflammation and degenerescence of tissues,and promoted it better.[Conclusions] EA Jianyu has anti-inflammatory effect and cuts back on the degree of degenerescence of tissues on scapulohumeral periarthritis model in rabbit.

Objective To analyze the significance of the waistline (WC), body mass index (BMI) and hyperinsulinaemia (HIns) on evaluating the metabolic syndrome. Methods The data of routine clinical examination were collected from the mid-aged and senile individuals (middle-senile group) who received oral glucose tolerance test (OGTT) for diabetes screening, and from a group of subjects who received annual OGTT for diabetes screening (mature group). Data were collected by a specialized person, input into a special computer database, and then analyzed with SAS 5.0 software by specialized staffs. Abnormal WC and BMI were determined according to IDF and CDS criteria. HIns was determined if fasting insulin (FIns) ≥15mU/L and 2-hour insulin after glucose burdening (2hPIns) ≥80mU/L. Results In the middle-senile group, the abnormality frequencies of WC and HIns as well as overall insulin level were obviously higher than that in the mature young group. The abnormality frequency of BMI was higher in mature young group. A higher detection rate of HIns existed in intolerance glucose test (IGT) and normal glucose test (NGT). The coincidence of WC and BMI diagnostic criteria were 77.5% and 74.3%, respectively, on evaluating with or without obesity in the middle-senile group and mature young group. When only WC criterion was used for evaluating the existence of insulin resistance, there would be 28.2% of missed diagnosis rate for MS patients. Conclusion WC, BMI and HIns are all risk factors for abnormal glucose metabolism, hypertension and dyslipidemia. There is different prevalence among different populations. The combination of WC, BMI and HIns might be more helpful for identifying MS at early stage.

Objective To investigate the prevalence of metabolic syndrome (MS) and its risk factors in the population of age between 21 to 78 years in Beijing, and to study the clinical significance of evaluation of MS and hyperinsulinaemia by the use of waist circumference and body mass index (BMI). Methods A standard questionnaire and 75g oral glucose tolerance test (OGTT) were used in the investigation. All the data, including plasma glucose (fasting blood glucose level and postprandial glucose level), blood levels of insulin, blood lipid, blood uric acid, and creatinine, as well as height, weight, waist circumference (WC), blood pressure, body mass, were determined or measured. Obesity was defined by WC and BMI value. The incidence of obesity complicated with two abnormal metabolic disorders (IDF), as well as hyperinsulinaemia in these subjects, were analyzed. Results The prevalence of obesity as determined by WC and BMI were 20.6% and 41.5%, respectively, in the said popalation of Beijing (P0.05) in subjects of obesity complicated with two other metabolic abnormality as defined by WC and BMI criteria, accounting for 1.37% and 3.13% of the whole investigated population (P

Objective To investigate the differences of coronary artery lesion between myocardial infarction patients with or without ST segment elevation. Methods This study included 126 confirmed myocardial infarction patients with acute ST segment elevation (STEMI) and 96 confirmed myocardial infarction patients with acute non-ST segment elevation (NSTEMI). All patients received coronary angiography and the results were compared between two groups. Results More myocardial patients were with single vessel disease in STEMI group as compared with NSTEMI group (44% vs 14%, P

[Objective]The intervention of electroacupuncture (EA) on chronic inflammation and cyclooxygenase-1/-2 mRNA expressions in rats with II collagen-induced arthritis was observed in vivo, so as to discuss the effect of EA on chronic inflammation and its pivotal mechanism. [Method]Wistar rats immunized intradermally with bovine type II collagen, were served as the animal models of chronic inflammation. EA stimulation, begun on day 11 after the injection, was applied at the acupoint equivalent to ST36 for continuous 10 times. Indomethacin and refecoxib were used respectively as the control treatments of NSAID inhibitor and COX-2 selective inhibitor. The arthritic index, swelling volume of paw and concentrations of prostaglandin E_2 (PGE_2) of local tissue were investigated. Furthermore, we investigated the impact of EA on the levels of COX-1 mRNA and COX-2 mRNA in synovial tissues and gastric mucosa of inflammatory rats by using reverse transcriptase-polymerase chain reaction (RT-PCR). [Results]All collagen-injected rats developed chronic inflammation of paws. EA remarkably reduced the paw swellings of rats on day 16 and day 21 and attenuated the severity of arthritis from the day 14 to day 21. The levels of PGE_2 in the inflammatory tissues of swollen paws were also lowered by EA. EA stimulation obviously down-regulated the expression of COX-2 mRNA in synovial tissue, but did not influence the level of COX-1 mRNA expressed in gastric mucosa. Indomethacin and refecoxib also strongly down-regulated COX-2 expression in synovial tissue. However, either indomethacin or refecoxib strongly affected the COX-1 mRNA expressions in gastric mucosa. [Conclusion] EA has a pronounced anti-inflammatory effect on collagen-induced arthritis, and the pivotal mechanism and also the advantage of its therapeutic result may be due to selective suppression of COX-2 gene activation, without destroying COX-1 activity in stomach.

In the present paper,the authors review progresses of researches on the protective effect of acupuncture in resisting acute myocardial ischemia-reperfusion injury in patients and animals from 1) resisting injury of oxygen-derived free radicals,2) reducing intracellular Ca2+ overload,3) relieving dysfunction of vascular endothelial cells of the myocardium,4) regulating immune functional activity and reducing inflammatory injury,5) reducing cardiocyte apoptosis,6) upregulating the expression of heat shock protein(HSP 70) in the myocardium,and 7) regulating activities of multiple cardiovascular protection factors and intracellular signaling pathways.The abovementioned results are mainly obtained from experimental animal studies.For this reason,the authors hold that the coming researches on acupuncture therapy for cardiovascular disorders should pay more attention to its clinical application,for example,controlled blood-pressure decline induced lower perfusion of the coronary artery,cardiac surgery(percutaneous tranluminal coronary angioplasty,intracardiac operation underdirect vision) etc.

Objective: To discuss the influence of comprehensive nursing intervention on patient’s oral mucositis with hematopoietic stem cell transplantation. Methods:One hundred and seventy four examples of patients with hematopoietic stem cell transplantation were equally and randomly divided into two groups, which are intervention group and control group. The usual care was given to the control group, while both the usual care and comprehensive nursing intervention were given to the intervention group. The comparative study of the occurrence and severity degree of oral mucositis in these two groups after one week pretreatment was conducted. Results:The occurrence rate of oral mucositis in intervention group and control group was 54.47%and 68.97%respectively. And there is no obvious significance of the occurrence rate before and after the intervention(x2=2.47, P>0.05). Nevertheless, the comprehensive nursing intervention could significantly lessen the severity of oral mucositis, and help to control the inflammation and promote healing. Conclusion:comprehensive nursing intervention could effectively reduce the occurrence degree of oral mucositis in the patients with hematopoietic stem cell transplantation.