Objective To investigate the role of sICAM-1 and TNF in the acute rejection of renal graft. Methods Serum sICAM-1 and TNF were sequentially determined in 41 patients with renal graft by ELISA and L_(929) target cell methods. Results The change of serum sICAM-1 was same as TNF, there was no significant difference in serum sICAM-1 and TNF among healthy controls, uremic patients, CsA toxioosis and stable transplantation. During acute rejection(AR) and infection episode, both serum sICAM-1 and TNF were obviously elevated. While the serum creatinine had no significant changes during infection, the serum sICAM-1 and TNF were elevated as early as 1～3 days before the rise of serum creatinine. Conclusion The serial assay of serum sICAM-1 and TNF would be helpful in the early diagnosis and differential diagnosis of AR.

BACKGROUND: Rhodiola rosco L acts on anti-aging,anti-fatigue and improving immunity in human body and improves angina pectoris.OBJECTIVE: To study the effect of rhodiola root on heart hemodynamics and myocardial contractility and probe into its mechanism.DESIGN: Observational and controlled based on the experimental animals.SETTING: Department of physiology in a medical university.MATERIALS: The experiment was performed in Laboratory of Cardiac Vessels of Department of Physiology of Guangxi Medical University,from January to April 2001.Thirty New Zealand white rabbits of either sex,weighted varied from 1.9 to 2.3 kg,were randomized into two groups,named as the control(physiological saline 4 mL/kg) and rhodiola sacra SH Fu (nuodikang) group(0.5 g/4 mL · kg-1),15 rabbits in each group. A hundred common wild frogs were employed,weighted varied from 120 to 160 g,randomized into 4 groups, named as the control group of physiological saline,nuodikang of small dosage group(blood concentration 209 mg/L),nuodikang of moderate dosage group(blood concentration 418 mg/L) and nuodikang of large dosage group(blood concentration 627 mg/L),25 frogs in each group.METHODS: ms2000 multiple medium biological signal analysis system was applied,by Straub method,frog heart perfusion specimen in vitro was prepared and the cardiac activity was assayed. The relevant indexes of ventricle activity in rabbits were determined with hemodynamics.MAIN OUTCOME MEASURES: Comparison of hemodynamics changes in rabbits of two groups and the comparison of effects of nuodikang at various dosages on frog heart work in vitro.RESULTS: Myocardial contractility in every nuodikang group was strengthened remarkably compared with the control(F = 8.939, P<0.01 ), the myocardial contractility in nuodikang groups at the dosages of 209 mg/L,418 mg/L and 627 mg/L was in the tendency of increasing,strengthened by 52. 3%,70. 1% and 86.4% respectively. But the significant difference was not apparent in the comparison among experimental groups(P>0.05). The significant difference in frog heart rates among groups was not apparent(P>0.05). Concerning to hemodynamics in rabbits,in nuodikang groups,the maximum increasing rate of pressure in the left ventricle was increased by 22.1% (F = 6.259,P<0.05),the mean pressure of artery was decreased by 8.2% (F = 5.688, P<0.05) . The significant difference in other indexes was not apparent in the comparison between the control and nuodikang groups.CONCLUSION: Nuodikang strengthens myocardial contractility and speeds up myocardial contraction, but does not present remarkable influence on myocardial dilation; simultaneously it reduces the mean pressure of artery.

Objective To observe the effect of modified electroconvulsive therapy combined with venlafaxine on cognitive function and its efficacy in patients with depression.Methods 60 patients with depressive disorder were selected,and they were randomly divided into study group and control group according to the random number table method,30 cases in each group.The patients in the study group accepted modified electroconvulsive therapy for 10 times combined with venlafaxine sustained-release tablets treatment,while the patients in the control group were only treated with venlafaxine.Both two groups were treated for 6 weeks.The cognitive function was assessed by the Repeat able Battery for the Assessment of Neuropsycholgical Status (RBANS) and the therapeutic effect was evaluated by Hamilton Depression(HAMD) Scale before and after treatment.Results The scores of immediate memory,delayed memory,speech function,visual breadth and attention of both two groups after treatment were significantly higher than those before treatment(all P ＜ 0.05),and the attention score of the study group was (109.96 ± 19.01) points,which was higher than (100.32 ± 17.13)points of the control group (t =2.062,P ＜ 0.05).The HAMD scores after treatment in both two groups were significantly lower compared with those before treatment (all P ＜ 0.05),and the HAMD score of the study group was (7.23 ± 4.19)points,which was lower than (10.27 ± 5.99)points of the control group (t =2.273,P ＜ 0.05).The total effective rate of the study group(96.7％) was significantly higher than that of the control group (86.7 ％) (x2 =6.546,P ＜ 0.05),and the cure rate of the study group (60.0％) was also higher than that of the control group (36.7％) (x2 =10.901,P ＜ 0.05).Conclusion Modified electroconvulsive therapy combined with venlafaxine treatment can effectively improve the cognitive function of depressive patients,and its effect is significantly better than monotherapy.

Objective To determine the expression of Caspase 8 and phospho-Akt(p-Akt)in condyloma acuminatum(CA)lesions, and to evaluate their significance. Methods Skin lesion samples were collected from 30 patients with CA, cancer tissue samples from 20 with cervical cancer, and normal skin samples from 20 healthy controls. All the samples were subjected to paraffin embedding. An immunohistochemical study was conducted to determine the expression and distribution of Caspase 8 and p-Akt in the above samples. Results The expression rate of Caspase 8 was significantly lower in CA lesions (23.33%)than in normal skin samples(90%, P < 0.01)and cervical cancer lesions(80%, P < 0.001). Moreover, the expression rate of p-Akt in CA lesions(93.33%)was significantly higher than that in the normal skin samples(90%, P<0.001), but lower than that in the cervical cancer lesions(95%, P<0.001). No significant correlations were observed between the expression of Caspase 8 and p-Akt in either CA lesions or normal skin samples. However, the expression of Caspase 8 was positively correlated with the expression of p-Akt in cervical cancer lesions(r=0.369, P<0.05). Conclusion Both suppressed apoptosis initiation of Caspase 8 and anti-apoptotic effect of p-Akt may be involved in the occurrence and development of CA.

AIM:To observe the effect of levcromakalim(Lev) on endthelin-1(ET-1) induced proliferation and the expression of protein kinase C(PKC) in cultured rat vascular smooth muscle cells(VSMCs).METHODS: VSMCs were isolated from rat aorta and were cultured with different concentrations of Lev in vitro after stimulated to proliferation by ET-1(10~(-8)(mol?L~(-1))).Vitality of VSMCs was detected by MTT;DNA replication was evaluated by 3H-TdR method.Flow cytometry was used to analyze cell cycle and apoptosis.The expression of PKC? protein and mRNA were determined by Western blot and reverse transcription-polymerase chain reaction(RT-PCR).RESULTS:Vitality of VSMCs and 3H-TdR intermingle rate were decreased(P

Objective To observe the effect of β-amyloid42 (Aβ42) on stimulating the inflammatory factors production by BV-2 microglia, including interleukin-1β (IL-1β), IL-10 and nitric oxide (NO), and to contrast the inhibitory action of anti-Aβ42 antibody in serum of Alzheimer's patients and the artificially synthesized anti-Aβ42 antibody. Methods The anti-Aβ42 antibodies were extracted from the serum of Alzheimer's patients. And the BV-2 microglia cells in murine were cultured as in vitro cell model. The cells were stimulated by Aβ42 and the two different anti-Aβ42antibodies to analyze the impact of stimulants on the cell activity. The Aβ42 of 5 μmol/L was added to the culture separately or in mixture with each of the two different anti-Aβ42 antibodies. Each antibody was mixed with Aβ42 of 5 μmol/L at final anti-Aβ42 antibody titre of 5 μg/ml, 1 μg/ml and 0.2 μg/ml,respectively. Then clear supernatant was collected from each tube respectively at 6 h, 12 h, and 24 h after culture, and the concentrations of IL-1β, IL-10 and NO were determined. Results The Aβ42,artificially synthesized anti-Aβ42 antibody and anti-Aβ42 antibody from Alzheimer's patients had no effects on the activities of BV-2 cells, the cell survival rates were (98. 6±5.8)%, (101.9±2.8)%and (98. 4±6.0)%, with no significant differences as compared with normal control group (F=0. 407, P＞0. 05). The inflammatory factors releasing from BV-2 cells stimulated by Aβ42 reached the peak level at 12 h, the concentrations of IL-1β, IL-10 and NO were (69.0±12.7) pg/ml, (24.1 ±4. 0) pg/ml and (128. 2±8. 7) μmol/L, the concentrations of IL-1β and NO were significantly higher at 12 h than at 6 h and 24 h (F= 15. 470 and 242. 107, P＜0.05), there was no significant difference in the concentration of IL-10 among 12 h, 6 h and 24 h (F=1. 852, P＞0.05). The two different antiAβ42 antibodies of different titre remarkably inhibited Aβ42 stimulated BV-2 cells to release inflammatory factors. At high titre of 5 μg/ml, the two different antibodies showed no significant difference in the inhibitory effects (P＞0.05), while at the titre of 0. 2 μg/ml, anti-Aβ42 antibody from Alzheimer's patients showed a significantly lower inhibitory effect than artificially synthesized antibodies, the concentrations of NO were (35.4 ± 2. 5) μoml/L and ( 19. 2 ± 3.3) μoml/L,respectively (P ＜ 0.05). Conclusions The Aβ42 can stimulate BV-2 microglia cells to release inflammatory factors. Anti-Aβ42 antibody from Alzheimer's patients has a lower inhibitory effect on Aβ42 in stimulating microglia to release inflammatory factors.

To explore the influence factors of hospital acquired pneumonia (HAP) in patients with cervical spinal cord injury(CSCI).Regression analysis the clinical data of 478 cases of patients with CSCI,the CSCI patients were divided into the infection group and non-infection group.Firstly,the single factor analysis was used to compare the difference of the influence factors between the two groups.And then,logistic regression analysis was used to explore the influence factors of HAP in patients with CSCI.122 suffered HAP in the 478 patients with CSCI,accounted for 25.52％.There's significant difference in injured segments,JOAS scores on admission,methylprednisolone treatment within 8 h after injury,tracheotomy,history of smoking and lung disease between the infection group and non-infection group(P ＜ 0.05).logistic regression analysis showed that the injured segments (OR =4.474),JOAS scores on admission (OR =3.856),tracheotomy (OR =2.452),history of lung disease (OR =1.589) were the independent influence factors of HAP in patients with CSCI.The incidence rate of HAP was high in patients with CSCI,there were a variety of influence factors for the HAP,and we should take targeted intervention measures so as to reduce the incidence of HAP.

[Objective] To observe the effect of Anxin Granules (mainly composed of Radix Ginseng, Ramulus Cinnamomi, Pericarpium Trichosanthis, Hinido, Poria, etc.) in improving the symptoms of angina pectoris (AP) and acute inflammatory reaction in coronary heart disease (CHD) patients with AP. [Methods] Eighty senile CHD patients with AP of blood-stasis type were allocated to groups A and B with random number table. Group A was treated with Anxin Granules 3.5 g, tid combined with routine drug treatment and group B with routine drug treatment only. Four weeks constituted one treatment course. The changes of symptoms, electrocardiogram (EGG) and serum hypersensitive C-reactive protein (Hs-CRP) level were observed before and after treatment. [Results] In relieving symptoms and decreasing Hs-CRP level, group A was better than group B ( P 0.05) . Serum Hs-CRP level decreased in groups A and B after treatment as compared with that before treatment (P

Objective To investigate the lymphocyte subsets,natural killer(NK) and soluble interleukin-2 receptors(SIL-2R) in syphilis patients with negative HIV and evaluate its clinical significance.Methods T-lymphocyte subsets,NK lymphocyte and SIL-2R were measured with flowcytometry(FCM) and enzyme linked immuno-sorbent assay(ELISA) from 60 cases of untreated group(28 cases),treated group(32 cases) and controls group(30 cases) in patients with latent syphilis.Results The percentage of CD_8 in untreated group(0.33?0.08) was markedly higher than controls group(0.25?0.04)(P

AIM:To analyse the risk factors of age, sex, course, best corrected visual acuity(BCVA),diopter and fundus features of high myopes with progressive high myopia. METHODS: A total of 167 patients with high myopes were categorized into four groups: group 1,age of 29 years or younger; group 2,between the age of 30 to 49 years; group 3,between the age of 50 to 69 years and group 4,age of 70 years or older. The refractive errors of all patients were measured without cycloplegia with an autorefractometer. Data of the spherical equivalent(SE) of the refractive errors in diopters (D)and fundus examed by direct ophthalmoscope were used in statistical analyses.RESULTS: The number of female was statistically larger than that of male(P＜0.01),also the disease course was correlated to the age. The visual acuity of high myopes significantly decreased as they grew older including the higher incidence of lacquer cracker, submacular hemorrhage, Fuchs spots, chorioretinal atrophy . CONCLUSION: Female maybe a risk factor of high myopia, advanced age is an important factor of visual acuity decrease. High myopes ought to be treated early to delay the progress of myopia and development of macular degeneration.