The mechanism of antiarrhythmic effects of palmatine( Pal), an benzoquinolizine compound were studied in experimental animals.In the isolated guinea pig papillary muscles, Pal 50 ?mol/L markedly prolonged the functional refractory period(FRP)and decreased the automaticity induced by adrenaline, but exerted no effect on excitabj- iity and contractility of the muscles.Pal(10 ?mol/L ) prolonged both APD20 and APD90 of the action potential of guinoa pig papillary muscles. The Vmax was decreased at 100 ?mol/L Pal, but the action potential amplitude( APA ) and resting potential ( RP ) were unaltered.The antiarrhythmic action of Pal may be due to its ability to decrease automaticity and to proloagate FRP and APD of the myocardium.

Genome replication of reovirus occurs in cytoplasmic inclusion bodies called viral factories or viroplasms. The viral nonstructural protein μNS, encoded by genome segment M3, is not a component of mature virions, but is expressed to high levels in infected cells and is concentrated in the infected cell factory matrix. Recent studies have demonstrated that μNS plays a central role in forming the matrix of these structures, as well as in recruiting other components to them for putative roles in genome replication and particle assembly.

Female ; Humans ; Adenomyosis/surgery* ; Endometriosis/diagnosis* ; Early Diagnosis ; Attention

CD3 Complex ; metabolism ; CD5 Antigens ; metabolism ; Diagnosis, Differential ; Female ; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor ; Humans ; Leukocyte Common Antigens ; metabolism ; Leukosialin ; metabolism ; Lymphoma, T-Cell, Cutaneous ; metabolism ; pathology ; Middle Aged ; Skin Diseases ; pathology

ObjectiveTo observe the therapeutic efficacy ofacupoint thread embedding in treating hyperlipidemia, and to explore a treatment method with valid efficacy and few side effects.MethodSixty patients with hyperlipidemia were randomized into a thread embedding group and a medication group, 30 cases ineach group. The thread embedding group was intervened by thread embedding at Zhongwan (CV12), Tianshu (ST25), Fenglong (ST40), and Zusanli (ST36), once a week; the medication group was by taking Xue Zhi Kang capsules, two capsules each time, twice a day. The two groups both received treatment for 8 weeks. The clinical efficacy, total cholesterol (Chol), and triglyceride levels were observed before and after treatment.ResultThe total effective rate was 86.7% in the thread embedding group versus 83.3% in the medication group, and the difference was statistically insignificant (P>0.05). After 4-week treatment, the transaminase level increased in 3.3% of the patients in the medication group, while no one showed an increased transaminase level in the thread embedding group. After 8-week treatment, the observed indexes were increased in the medication group, while those in the thread embedding group were still stable.ConclusionAcupoint thread embedding is effective in treating hyperlipidemia, and the effect is durable with few side effects.

Jat(lOmg/kg iv) administered 5 min prior to ligation of left anterior descending coronary artery significantly decreased the incidence of arrhy-thmias(VT and VF)and mortality of rats during ischaemia and reper-fusion.It also delayed the onset and shortened the duration of arrhythmias. The size of myocardial infarction caused by coronary artery ligation was decreased by Jat ( 0.75mg/kg iv. 1 h before and 6 h after operation) in rabbits.

BACKGROUND: Oscillating field stimulation (OFS) is an emerging technology that has been used to treat spinal cord injury (SCI) with promising therapeutic effects. The application of electric current to the injured spinal cord is known to promote healing and tissue regeneration. However, the mechanisms by which OFS leads to SCI recovery are not yet fully understood. OBJECTIVE: To investigate whether OFS can promote the recovery of neurological function by reducing neuroinflammatory responses after SCI in rats. METHODS: Sixty-three female Sprague-Dawley rats were randomized into an experimental group, a SCI group and a sham operation group. Rats in the sham operation group only underwent laminectomy without damaging the spinal cord, and animal models of thoracic (T9-T10) SCI were established in the SCI and experimental groups. The OFS device was implanted into the injured site in the experimental group, followed by continuous electrical stimulation. Basso-BettieBresnahan score was used to evaluate the recovery of motor function after SCI in rats. In addition, hematoxylin-eosin staining was used to test tissue repair and inflammatory cell recruitment. The number of astrocytes and microglia was examined by immunofluorescence staining. The study protocol was approved by the Animal Ethics Committee of Anhui Medical University, with an approval No. LLSC20190736. RESULTS AND CONCLUSION: The Basso-Bettie-Bresnahan score of the experimental group was significantly higher than that of the SCI group. Hematoxylineosin staining results showed that the number of inflammatory cells in the injured site was considerably reduced in the experimental group compared with the SCI group. The results of immunofluorescence detection showed that the number of glial fibrillary acidic protein positive cells and microglia in the experimental group was dramatically lower than that in the SCI group at different time after surgery. These findings indicate that OFS can improve the motor function of lower limbs, promote tissue repair, reduce inflammatory cell recruitment, and inhibit the activation of astrocytes and microglia after spinal cord injury in rats. OFS can promote the recovery of neurological function by inhibiting inflammatory response after SCI.

Early diagnosis of hepatocellular carcinoma(HCC)is still a great challenge in clinical practice.Tumor markers such as alpha-fetoprotein(AFP),liver enzymes,cytokines,and some special glycoproteins,though helpful,are not sensitive and specific enough for early diagnosis of HCC.The establishment of several interesting predictive diagnostic models on liver fi- brosis/cirrhosis suggests that mathematic predictive model,which is developed based on large sample size and follow-up study, might be of higher sensitivity,specificity and feasibility in clinical application.Here we suggest that more attention should be paid to this kind of multi-parameter predictive diagnostic models clinically,so as to improve the early diagnosis of HCC in a more economical and feasible way.

Adenoma ; surgery ; Endoscopy ; Humans ; Pituitary Neoplasms ; surgery

Objective To study the joint effect of small dosages of Aspirin and compound Danshen dropping pills on type 2 diabetes(T2DM) patients'and impaired glucose tolerance(IGT) patients' hypercoagulation blood.Methods The study followed diagnostic standards for T2DM by World Health Organization (WHO) in 1999,and randomly selected two groups of clinical patients:one had 63 patients with IGT and the other contained 68 patients with T2DM.Both groups took compound Danshen dropping pills with the total dosage of 10 pills each time,3 times per day,for continuum of 3 months.After that both groups stopped medication for 7 days,then began taking Aspirin after meal,with the dosage of 100 mg per day,for a continuum of 3 month.After that both groups stopped the medication for 7 days,then both group continued to take same dosage of ASA with addition of compound Danshen dropping pills of total amount of 10 pills per each time,3 times per day,for acontinuum of 3 months.Each medication period (ev ery 3 months) was monitored for platelet count (PET),prothrombin time (PT),kaolin partial thromboplastin time (APPT),The effective rate of anticoagulation for both groups were then calculated.Results The difference of PT、APTT between before and after taking single dosage of ASA for patients with impaired glucose tolerance (IGT) was statistically significant (P＜0.05),while patients with T2DM had no significant difference before and after taking same single dosage of ASA.Similarly, both groups with single dosage of compound Danshen dropping pills had no significant difference between before and after taking the medication.However,the difference of PT、APTT between before and after taking the combination of Aspirin and Danshen dropping pills of both groups were statistically significant (P＜0.05).IGT group with single dosage of ASA had an effective rate of 71.4％ for anti coagulation,while T2DM group with the same dosage reached 23.8％.The IGT group with combinatory dosage of ASA and Danshen dropping pills reached an effective rate of 100％ for anticoagulation,while T2DM group with the same dosage was 86.7％.The difference between those effective rates of anticoagulation was statistically significant in different medicine takeways of two groups (P＜0.05).Conclusion The combinatory use of small dosage of Aspirin with compound Danshen dropping pills had evident effect to improve IGT and hypercoagulation blood of T2DM patients who had good control of their blood glucose.