1.Clinical observation on focused ultrasound in the treatment of cervical erosion
Chinese Journal of Primary Medicine and Pharmacy 2013;20(4):511-512
Objective To investigate the clinical effects of focused ultrasound in the treatment of cervical erosion.Methods 417 patients with cervical erosion were treated with focused ultrasound(Haijixing treatment instrument),and followed up for 3 months to observe the efficacy and adverse reactions.Results In total 417 cases,321cases(76.98%) were cured,and there were effective in 95 cases(22.78%),ineffective in 1 case(0.24%),and the total effective rate was 99.76%.No serious adverse reactions were founded.Conclusion Focused ultrasound(Haijixing treatment instrument) in the treatment of cervical erosion has obvious effect and mild adverse reactions.
2.Efficacy and safety of low-dose mitomycin-C in the treatment of pterygium
International Eye Science 2008;8(11):2178-2181
·AIM:To observe the efficacy and the safety of low-dose mitomycin-C (MMC) in the treatment of pterygium.·METHODS:In a prospective,randomized and controlled clinical trial,a total of 550 eyes of 400 patients diagnosed with pterygium were randomly divided into the four groups-group 1:130 eyes of 100 patients,without MMC;group 2:140 eyes of 100 patients,with 0.2g/L MMC;group 3:150 eyes of 100 patients,with 0.3g/L MMC;group 4:130 eyes of 100 patients,with 0.4g/L MMC.They underwent the excision of primary pterygium barely the sclera.Visual acuity,intraocular pressure (IOP),extraocular movement,iridocyclitis,scarring, symptoms (tearing,photophobia,foreign body sensation) and signs (conjun-ctival hyperemia,ulcer of sclera and cornea,perforation of sclera) of subjects,were recorded on the day of enrollment,and after the surgery,on 2 weeks,2 months,6 months,and 1 year.·RESULTS:In group 3 and group 4,the recurrence of pterygium after the excision were less than that in group 1 and group 2 ( P < 0.01 ).In group 4,after the surgery,there were more probability of raising lOP,iridocyclitis,symptoms and signs than that in group 1,2,and 3.·CONCLUSION:Low-dose MMC is effective in the treatment of preventing the recurrence of primary pterygium.Its efficacy rises along with its density.And meantime,the safety of the treatment of low-dose MMC declines.
3.Relationship between Barrett's oesophagus and Helicobacter pylori infection
Journal of Third Military Medical University 1983;0(04):-
005) The prevalence of Hp infection in antrum was significantly lower in patients with GERD (16/50) and BE (33/106) than that in control subjects (30/50,P
4.Effect of Pingchuanning on the Levels of LTB4 and sICAM-1 of Guinea Pigs with Asthma
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To observe the effect of Pingchuanning on the levels of LTB4 and sICAM-1 of guinea pigs with asthma. Methods The model guinea pigs of asthma were sensitized with ovalbumin (OVA),the changes of the delitescence of asthma was observed. The levels of LTB4 in serum and sICAM-1 in bronchoalveolar lavage fluid (BALF) were also observed by means of enzyme-linked immunosorbent (ELISA). Result Comparing with the model group,the delitescence of asthma was obviously prolonged in guinea pigs treated by Pingchuanning. The levels of LTB4 and sICAM-1 of guinea pigs with asthma treated by Pingchuanning were significantly lowered (P
5.Use of Xingnaojing Injection in Medical Insurance Inpatients in Beijing:Survey and Analysis
China Pharmacy 1991;0(02):-
OBJECTIVE: To evaluate the use of Xingnaojing Injection in medical insurance inpatients in Beijing city so as to provide reference for rational drug use and medical insurance billing.METHODS: Inpatient cases using Xingnaojing Injection in 5 Beijing hospitals of different levels were randomly sampled from the 2004 Beijing Medical Insurance Database,and analyzed in terms of indication, dosage, treatment course and ADR.RESULTS: A total of 676 cases were sampled with more than 25 kinds of indications,and among the top 10 indications, 18.0% were inconsistent with the instruction,and 17.5% were in lack of evidence support such as randomized controlled trials.Individual dose of Xingnaojing Injection was(27.9?9.0)mL, which was higher than the usual dose described by the instructions and clinical trials.The mean treatment course of Xingnaojing Injeciton was 14.6 days,with 20.9% exceeding 20 days.Drugs used in combination with Xingnaojing Injection included anti - thrombosis agents( 13.3% ) and traditional Chinese medicines with the action of promoting blood circulation and removing blood stasis (12.7%) .CONCLUSION: The use of Xingnaojing injection in medical insurance patients was not consistant with the instruction or evidence supported by clinical trials.
6.All-trans retinoic acid-induced nerve cell differentiation of rat bone marrow mesenchymal stem cells inhibits T lymphocyte proliferation
Chinese Journal of Tissue Engineering Research 2009;13(40):7889-7892
BACKGROUND:Several studies have demonstrated that bone marrow mesenchymal stem cells (BMSCs) inhibit the proliferation and activation of T lymphocytes, exerting a role of negative immunological regulation. OBJECTIVE: To induce rat BMSCs to differentiate into neuron-like cells in vitro using all-trans retinoic acid and to investigate the effects of differentiated cells on T lymphocyte proliferation.DESIGN, TIME AND SETTING: A cytological in vitro observation was performed in Guangdong Medical College in March 2008.MATERIALS: Two male 4-week-old Sprague Dawley rats were provided by the Laboratory Animal Center of Guangdong Medical College. Fresh healthy human blood was provided by the Department of Clinical Laboratory, Guangdong Medical College. METHODS: Rat BMSCs were isolated by adherent method and were passaged in vitro. Cells of passage 5 were pre-induced with Low glucose-dulbecco's modified eagle's medium (LG-DMEM) containing 0.3 mg/L all-trans retinoic acid and 10% fetal bovine serum. Twenty-four hours later, aforementioned LG-DMEM was discarded and LG-DMEM containing 0.6 mg/L all-trans retinoic acid was added to induce cells differentiation into neuron-like cells. Fresh healthy human blood was taken to prepare responding cells. Rat BMSCs, as stimulator cells, were included for one-way mixed lymphocyte reaction. Four groups were designated. Control group: 100 μL of responding cells at a concentration of 1×109/L; Experimental group 1:1×104 neuron-like cells + 100 μL of responding cells; Experimental group 2:1×105 neuron-like cells + 100 μL of responding cells; Experimental group 3:1×106 neuron-like cells + 100 μL of responding cells.MAIN OUTCOME MEASURES: Morphological observation of induce-differentiated cells, identification of nerve cells, and one-way mixed lymphocyte reaction results.RESULTS: Fifty minutes after addition of inducing medium, under the optical microscope, BMSCs exhibited a typical morphology of perikaryon. Three hours later, most of cells became into bipolar or multipolar neuron-like cell appearance with cell bodies and processes. Immunocytochemical staining results showed that majority of cells exhibited neuron specific enolase- and nestin-positive expression and glial fibrillary acidic protein-negative expression. Compared with the control group, radionuclide counts per minute were significantly reduced in each experimental group (P<0.05). There was significant difference in radionuclide counts per minute between experimental groups (P<0.05). With increasing BMSCs amount, the inhibitory effects on T lymphocyte proliferation were more evident.CONCLUSION: AII-trans retinoic acid can induce neuron-like cell differentiation of rat BMSCs in vitro. The neuron-like cells can inhibit human T lymphocyte proliferation in a dose-dependent manner.
7.CIinicaI anaIysis of 54 cases misdiagnosed as optic neuritis
International Eye Science 2015;(3):546-548
· AlM: To analyze the clinical data of 54 cases misdiagnosed as optic neuritis and to explore measures to reduce misdiagnosis
· METHODS: This retrospective study comprised 54 patients that had been misdiagnosed as optic neuritis from September 2000 to June 2013. The misdiagnosis features were summarized.
· RESULTS:Many diseases can easily be misdiagnosed as optic neuritis, including ischemic optic neuropathy, intracranial tumors, optic nerve vasculitis, myelinated nerve fibers, and so on.
· CONCLUSlON: The measures to reduce misdiagnosis consisted of detailed history collection, perfect physical examination and comprehensive expertise of fundus disease.
8.The clinical manifestations and genetic analysis of lisencephaly in children
Journal of Clinical Pediatrics 2017;35(7):519-521
Objective To explore clinical characteristics and pathogenic gene of lisencephaly. Methods The clinical manifestation, laboratory examination and gene detection results of lisencephaly in two children were analyzed retrospectively, and relevant literature were reviewed. Results Two male children with lisencephaly are at ages of 7 months, and 3 years and 4 months respectively. Both of them were admitted to hospital due to epilepsy and loss of consciousness at the time of attack. There was no obvious abnormality in laboratory examination. Both of their EEG indicated epileptic wave. Cranial MRI showed lissencelphaly. Gene analysis showed that there was a heterozygous mutation of IVS3-1G>A in PAFAH1B1 gene in a child, which resulted in the deletion of exon 4 in mRNA level by functional analysis. No mutations were found in the parents of the child. The other one had c.274A>G mutation (p.K92E) in PAFAH1B1 gene, which has not been reported before, and his parents were normal. Conclusion Patients with lissencelphaly may combine with epilepsy, and the PAFAH1B1 gene mutation is the common cause.
9.The effects of the treatment of early cracked teeth with vital pulp preservation
Journal of Practical Stomatology 2016;32(5):664-666
Objective:To investigate the effects of the treatment of early cracked teeth with vital pulp preservation.Methods:63 early cracked teeth with vital pulp in 56 patients were treated by light cured composite resin filling with or without MTA rebasing,and restored by precious metal crown.The patients were followed up for 2 years.Results:After 2 years follow-up,57 teeth were successfully treated and failure was found in 6 teeth,the effective rate was 90.4%.Conclusion:Light cured resin filling directly or with MTA rebasing for early cracked teeth with vital pulp,and restoration by precious metal crown are effective in the treatment of early cracked teeth with vital pulp.
10.Pharmaceutical Care for One Case of Multi-target Treatment for Refractory ClassⅣ+ⅤLupus Nephritis
China Pharmacy 2015;(29):4158-4159,4160
OBJECTIVE:To summarize the method and experience of clinical pharmacist participating in pharmaceutical care for a case of refractory class Ⅳ+Ⅴ lupus nephritis (LN). METHODS:Clinical pharmacists and physicians formulated individualized treatment together,i.e. prednisone 30 mg/d+MMF 0.5 g,q12 h+FK506 1 mg,q12 h;on the fifth day of treatment,the patient suf-fered from ardent fever and increase of blood pressure,and clinical pharmacist suggested using linezolid 0.6 g,ivdrip,q12 h+merope-nem1.0 g,ivdrip,q8 h to control pulmonary infection;using Amlodipine besylate tablet 5 mg,po,qd+one Valsartan hydrochlorothia-zide capsule, po,qd+Arotinolol hydrochlorid tablet 10 mg,po,bid to control blood pressure. RESULTS:Multi-target treatment for re-fractory class Ⅳ+Ⅴ lupus nephritis achieved efficacy:the patient got higher plasma albumin and lower 24 h urine protein quantity af-ter 14 days;the patient suffered from secondary pulmonary infection and high blood pressure;physicians adopted the pharmacist’s suggestion so that pulmonary infection and blood pressure had been controlled. CONCLUSIONS:To ensure the effectiveness and safe-ty of the multi-target treatment,clinical pharmacists should formulate individualized treatment,focus on the possible ADR and provide phamaceutical care in the field of blood pressure monitoring,infection control,etc. when participating in multi-target treatment.